Cumhuriyet Science Journal最新文献_第4页

Spectrophotometric Determination of Atorvastatin Based on Charge Transfer Reaction with Quinalizarin 基于与喹哪嗪的电荷转移反应的阿托伐他汀分光光度测定法

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1327163

Fatih Yi̇ği̇t, Nurettin Tarkan, Mehmet Akdeni̇z, Figen Erek, I. Aydin

引用次数: 0

Evaluation of the Interactions of Cabozantinib with Topoisomerase Enzymes by in vitro Enzyme Activity Assays, and its Effects on Cancer Cell Proliferation 通过体外酶活性测定评估卡博替尼与拓扑异构酶的相互作用及其对癌细胞增殖的影响

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1376788

Feyza Oflaz, Naz Ünal, Burcin Gungor, Pakize Cantürk

{"title":"Evaluation of the Interactions of Cabozantinib with Topoisomerase Enzymes by in vitro Enzyme Activity Assays, and its Effects on Cancer Cell Proliferation","authors":"Feyza Oflaz, Naz Ünal, Burcin Gungor, Pakize Cantürk","doi":"10.17776/csj.1376788","DOIUrl":"https://doi.org/10.17776/csj.1376788","url":null,"abstract":"The discovery of many drugs in recent years provides a definitive solution in the treatment of various diseases, but today, despite the discovery of many effective anticancer drugs, there are various types of cancer that have limitations in treatment and are still not completely curable. Since most of these limitations are due to cancer cells gaining resistance or compounds only being effective in certain types of cancer cells, the search for more effective anticancer drugs that are also effective in these types of cancer is inevitable. Cabozantinib is in medical use as a highly effective anticancer drug in various types of cancer, such as medullary thyroid cancer and kidney cancer. The anticancer properties of the Cabozantinib compound have attracted more attention in recent years, however, more studies are needed to define the anticancer activities of this compound. In our study, the interactions of Cabozantinib with topoisomerase enzymes, were demonstrated through in vitro enzyme activity tests, and the anti-proliferative effect of Cabozantinib was studied on MCF7, A549 and PC3 cell lines. By analyzing the interactions of the Cabozantinib with topoisomerases, the action mechanisms of the compound at the molecular level was evaluated.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"16 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction 通过云点萃取同时对痕量水平的 B1、B9 和 B12 维生素进行高效液相色谱分析

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1342960

H. I. Ulusoy, İpek Nur Yi̇ği̇t, Ümmügülsüm Polat, Esra Durgun, Aslıhan Gürbüzer, Songül Ulusoy

{"title":"Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction","authors":"H. I. Ulusoy, İpek Nur Yi̇ği̇t, Ümmügülsüm Polat, Esra Durgun, Aslıhan Gürbüzer, Songül Ulusoy","doi":"10.17776/csj.1342960","DOIUrl":"https://doi.org/10.17776/csj.1342960","url":null,"abstract":"An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the HPLC analysis. All parameters affecting the CPE method such as pH, buffer volume, incubation time, surfactant and electrolyte concentration, solvent for the surfactant-rich phase and its amount have been individually studied and optimized step by step. After the optimization of all parameters of the CPE process, the detection limits of the developed method for B1, B9 and B12 vitamins were calculated as 1.42 ng mL-1, 7.14 ng mL-1 and 14.28 ng mL-1 , respectively. The linear working ranges for three vitamin molecules was obtained in the range of 5.0-500.0 ng mL-1. After CPE procedure, determination of vitamin molecules was carried out by using HPLC system with diode array detector(DAD) at 244 nm for vitamin B1, 285 nm for vitamin B9, and 361 nm for vitamin B12, respectively","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"47 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidroksiklorokin Prostat Kanseri Hücrelerinde m6A RNA Metilasyonunu Düzenler 羟氯喹可调控前列腺癌细胞中的 m6A RNA 甲基化

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1307100

Sevinc Yanar, Merve Gülsen BAL ALBAYRAK

{"title":"Hidroksiklorokin Prostat Kanseri Hücrelerinde m6A RNA Metilasyonunu Düzenler","authors":"Sevinc Yanar, Merve Gülsen BAL ALBAYRAK","doi":"10.17776/csj.1307100","DOIUrl":"https://doi.org/10.17776/csj.1307100","url":null,"abstract":"Prostate cancer ranks as the second most prevalent cancer in men globally. One of the evolving subjects of investigation in prostate cancer is the role of N6-methyladenosine (m6A) modifications. Hydroxychloroquine (HCQ), an autophagy inhibitor, was shown to be promising in enhancing the response to chemotherapy in prostate cancer. The interplay between autophagy and m6A is an emerging area of research. However, the relationship between m6A modifications and HCQ remains unclear. The objective of this study was to examine the effect of HCQ on the regulation of m6A methylation in prostate cancer. Initially, the cytotoxic effect of HCQ on LNCaP and PC3 cells was evaluated. The IC50 values for each cell were calculated. Finally, m6A levels in HCQ-treated and untreated cells were determined using m6A RNA methylation quantification kit. HCQ showed a significant dose- and time-dependent reduction in cell viability. Following HCQ treatment, a statistically significant decrease in m6A levels was observed: from 0.050±0.001% to 0.013±0.02% in PC3 cells and from 0.039±0.001% to 0.016±0.01% in LNCaP cells. The study unveils for the first time that HCQ affects m6A methylation in prostate cancer. The impact of autophagy inhibitor HCQ on m6A modifications introduces a novel dimension to its potential mechanisms of action.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"48 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Characterization of Tung Oil Loaded Melamine Formaldehyde Microcapsules 桐油载体三聚氰胺甲醛微胶囊的制备与表征

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1299101

Tülin GÜRKAN POLAT, Ahmet Gençer, Meltem Asi̇ltürk, Yılmaz Aksu

{"title":"Preparation and Characterization of Tung Oil Loaded Melamine Formaldehyde Microcapsules","authors":"Tülin GÜRKAN POLAT, Ahmet Gençer, Meltem Asi̇ltürk, Yılmaz Aksu","doi":"10.17776/csj.1299101","DOIUrl":"https://doi.org/10.17776/csj.1299101","url":null,"abstract":"In this study microcapsules were prepared by in-situ polymerization route with melamine formaldehyde as a shell material and tung oil as core material. Melamine formaldehyde (MF), a thermosetting polymer, is one of the most widely used monomers in microencapsulation due to its superior mechanical strength and thermal stability. Tung oil contains unsaturated double bonds that can be oxidized to form a film in air. Tung oil is fast drying and biodegradable, besides it is low cost and does not pollute the environment. Most importantly, tung oil is a versatile substance in industry. Therefore, tung oil is a good choice as core material. The chemical structure of microcapsules were characterized by Fourier Transform Infrared (FTIR) spectroscopy. The surface morphology and particle size and distribution were evaluated by Scanning Electron Microscopy (SEM). The thermal behavior of microcapsules and tung oil were studied by thermogravimetric analysis (TGA). The results showed that the spherical microcapsules (particle size of mostly 4-5 μm) were produced with a filling content of 15.64 wt.%, and a yield of 49.78 wt.%. The microcapsules exhibit a good thermal stability","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"276 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line 评估 PI3K/AKT/MTOR 通路抑制剂奥米帕利对伯基特淋巴瘤细胞系的抗肿瘤活性

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1344535

Zekeriya Keski̇n, Fatih Yulak, Hatice Terzi̇, M. İnanir

{"title":"Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line","authors":"Zekeriya Keski̇n, Fatih Yulak, Hatice Terzi̇, M. İnanir","doi":"10.17776/csj.1344535","DOIUrl":"https://doi.org/10.17776/csj.1344535","url":null,"abstract":"There are many challenges in the treatment of Burkitt lymphoma, especially in immunocompromised individuals, elderly patients, and patients with relapsed or refractory disease. Therefore, there is a need for new and less toxic therapeutic agents. The aim of this study was to determine the antitumoral activity of omipalisib, a PI3K/AKT/mTOR pathway inhibitor, in the Burkitt lymphoma. Raji cell line was used in the study. Omipalisib was administered to the cell line and then the cytotoxic effect of omipalisib on Raji cells was evaluated by the XTT test. The IC50 value was calculated according to the results of the XTT test. Apoptosis and cell cycle experiments were studied with the calculated IC50 value. The flow cytometric method was used to determine the effect of omipalisib on apoptosis and cell death. The results of the study showed a statistically significant cytotoxic effect of increasing concentrations of omipalisib on Raji cells. The apoptosis experiment performed revealed that omipalisib strongly induced apoptosis. The cell cycle experiment showed that omipalisib stimulated the cell cycle arrest at the G0/G1 phase. It was concluded that omipalisib exhibited antitumoral activity on Burkitt lymphoma cells with its cytotoxic effect and induced apoptosis and cell cycle arrest. Considering this effect, targeting the PI3K/AKT/mTOR pathway with omipalisib can be a new treatment option.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"266 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Triazolopyrimidinone Derivative: A Portable Electrochemical Approach to Investigate DNA Interactions 一种新型三唑嘧啶酮衍生物：研究 DNA 相互作用的便携式电化学方法

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1344756

Arif Engin Çeti̇n

{"title":"A Novel Triazolopyrimidinone Derivative: A Portable Electrochemical Approach to Investigate DNA Interactions","authors":"Arif Engin Çeti̇n","doi":"10.17776/csj.1344756","DOIUrl":"https://doi.org/10.17776/csj.1344756","url":null,"abstract":"In this study, a novel triazolopyrimidinone derivative, 2-(2-chlorophenyl)-5-(morpholinomethyl)-[1,2,4]triazolo[1,5-a]pyrimidin-7(3H)-one, abbreviated as CPD-1, was synthesized as a drug candidate. By employing electrochemical techniques, we analyzed the electrochemical behavior of this compound and its interactions with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Experimental parameters such as pH, concentration, scan rate, immobilization time were studied using Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV) to obtain the most precise analytical signals. We present an innovative approach to evaluate the toxicity effect of this drug candidate on DNA. We also propose a simplified equation to quantify toxicity effects based on changes in electrochemical signals, specifically peak current of guanine bases, before and after drug-DNA interactions. Our methodology contributes to the burgeoning field of electrochemical toxicity assessment and holds promise for advancing drug development and safety evaluation. Furthermore, stability tests for the drug candidate were conducted on different days. Notably, our investigation revealed significant alterations in guanine bases upon the interaction of CPD-1 with both ssDNA and dsDNA, underscoring the potential impact of these compounds on DNA structure. Based on our experimental data, we conclude that this molecule can be utilized as a drug due to its effects on DNA.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"224 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139153026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Methanol Electro-oxidation on Ni, S Dual Doped Reduced Graphene Layer Catalyst 在镍、S 双掺杂还原石墨烯层催化剂上实现高效甲醇电氧化

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1278386

Rukan Suna, Sedef Kaplan

引用次数: 0

Green Synthesis of CuO Nanoparticles Using Tragopogon porrifolius and Their Antioxidant and Photocatalytic Applications 利用猪笼草绿色合成氧化铜纳米粒子及其抗氧化和光催化应用

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1329389

Gamze TOPAL CANBAZ

引用次数: 0

MW Assisted Synthesis of New 4,6-diaryl-3,4-Didhydropyrimidines-2(1H)-thione Derivatives: Tyrosinase Inhibition, Antioxidant, and Molecular Docking Studies MW 辅助合成新的 4,6-二芳基-3,4-二氢嘧啶-2(1H)-硫酮衍生物：酪氨酸酶抑制、抗氧化和分子对接研究

Cumhuriyet Science Journal Pub Date : 2023-12-28 DOI: 10.17776/csj.1299843

S. Fandaklı, Tayyibe Beyza Yücel, Elif Öztürk, U. Uzuner, Burak Barut, Fatih Mehmet Ateş, N. Yaylı

{"title":"MW Assisted Synthesis of New 4,6-diaryl-3,4-Didhydropyrimidines-2(1H)-thione Derivatives: Tyrosinase Inhibition, Antioxidant, and Molecular Docking Studies","authors":"S. Fandaklı, Tayyibe Beyza Yücel, Elif Öztürk, U. Uzuner, Burak Barut, Fatih Mehmet Ateş, N. Yaylı","doi":"10.17776/csj.1299843","DOIUrl":"https://doi.org/10.17776/csj.1299843","url":null,"abstract":"A number of new methoxy-substituted 4,6-diaryl-3,4-dihydropyrimidine-2(1H)-thiones (DH-Pyr-S, 17-28) were designed and synthesized by the reaction of methoxy-substituted chalcones (1–14) with thiourea using solid-phase microwave method (MW) in view of the structural requirements as suggested in the pharmacophore model for tyrosinase inhibition (TI). Synthesized compounds were assessed for their in vitro TI potential and compounds 16, 17, and 21 exhibited notable tyrosinase inhibitory properties at the concentrations of 31.86 ± 2.45 µM, 44.58 ± 0.46 µM, and 48.47 ± 0.66 µM, respectively. Compounds (16, 17, and 21) were exhibited experimentally more potent TI than the standard used in terms of the IC50 value (Kojic acid, 55.38 ± 2.30 µM; p<0.0001). Additionally, DPPH activity of 15-28 were evaluated and compound 17 showed the moderate DPPH activity (45.64 ± 0.34%). Binding affinities of synthesized molecules to the tyrosinase catalytic core were further investigated through in silico molecular docking studies using AutoDock Vina (version 1.2.5), discovery studio accelyrs (BIOVIA, Dassault Systèmes) and predicting small-molecule pharmacokinetic properties using graph-based signatures (pkCSM) programs were used for ADMET calculations. Among synthesized compounds 15, 21, and 24 revealed high binding affinity to tyrosinase active site with lowest binding free energy (ΔG) values of -7.9 kcal/mol, thereby outperformed kojic acid affinity. In conclusion most modeling results were in agreement with their experimental data, suggesting the TI potential of lead compounds.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"15 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0