Cumhuriyet Science Journal最新文献

Synthesis of Isatin Monohydrazone, Spectroscopic Analysis, DFT Studies, and Molecular Docking Applications to MCF-7 Cell Line Isatin单腙的合成、光谱分析、DFT研究及其在MCF-7细胞系上的分子对接应用

Cumhuriyet Science Journal Pub Date : 2025-03-25 DOI: 10.17776/csj.1500189

Ceylan Alkaya Yıldız, Sultan Erkan

引用次数: 0

Multispectral Imaging and Single-Cell Analysis with Genetically Encoded Biosensors Unveil Complex Interactions between Extracellular ATP and Intracellular Calcium 多光谱成像和单细胞分析与基因编码的生物传感器揭示细胞外ATP和细胞内钙之间复杂的相互作用

Cumhuriyet Science Journal Pub Date : 2024-06-30 DOI: 10.17776/csj.1462654

Emrah Eroğlu

{"title":"Multispectral Imaging and Single-Cell Analysis with Genetically Encoded Biosensors Unveil Complex Interactions between Extracellular ATP and Intracellular Calcium","authors":"Emrah Eroğlu","doi":"10.17776/csj.1462654","DOIUrl":"https://doi.org/10.17776/csj.1462654","url":null,"abstract":"The dynamic interplay between extracellular ATP (eATP) and intracellular calcium ([Ca2+]i) serves as a pivotal signaling axis in cellular physiology, influencing a myriad of cellular processes. Traditionally recognized as an energy currency within the cell, ATP has emerged as a multifunctional signaling molecule that orchestrates diverse cellular responses through activation of purinergic receptors. The complex link between ATP signaling and calcium dynamics plays a central role in cellular communication and homeostasis. Advancements in imaging technologies such as development of genetically encoded biosensors have revolutionized the study of cellular signaling dynamics, enabling visualization of the spatiotemporal aspects of eATP and [Ca2+]i in real-time. The convergence of eATP signaling and [Ca2+]i dynamics serves as a central hub in cellular communication. In this study, utilizing bicistronic construct biosensors for multispectral imaging of [Ca2+]i responses to eATP, we show that distinct concentrations of eATP administration reveal complex intracellular [Ca2+]i responses, potentially attributed to receptor desensitization. Single-cell co-imaging uncovers [Ca2+]i heterogeneity, emphasizing the significance of individual cell dynamics in eATP-induced calcium signaling. Therefore, this study sheds light on the intricacies of eATP-induced calcium signaling, providing insights valuable for basic research and therapeutic applications.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"45 2","pages":"360-365"},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://csj.cumhuriyet.edu.tr/en/download/article-file/3837143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of Bromophenol Blue from Aqueous Solution Using Bentonite, Zeolite and Graphene Oxide 使用膨润土、沸石和氧化石墨烯去除水溶液中的溴酚蓝

Cumhuriyet Science Journal Pub Date : 2024-03-23 DOI: 10.17776/csj.1424592

Nuket Kartal Temel, İbrahim Gökçe Erdem

{"title":"Removal of Bromophenol Blue from Aqueous Solution Using Bentonite, Zeolite and Graphene Oxide","authors":"Nuket Kartal Temel, İbrahim Gökçe Erdem","doi":"10.17776/csj.1424592","DOIUrl":"https://doi.org/10.17776/csj.1424592","url":null,"abstract":"In this study, an anionic dyestuff bromophenol blue (BrPB) from the aqueous solution was removed by adsorption in the presence of bentonite (B), zeolite (Z) and graphene oxide (GO). The effects of pH, adsorbent amount, initial concentration of BrPB, temperature and time on adsorption were investigated. After the adsorption process, ultraviolet-visible region spectrophotometer (UV-VIS) was used to measure the amount of dyestuff remaining in the solution which is not adsorbed. It’s found that; the optimum values obtained for the adsorption process; the optimum pH value 5 for B and Z, 4 for GO; 0.03 g amount of adsorbent and 60 minutes for the equilibrium time. The compatibility of Langmiur and Freundlich isotherm models was found as Z> GO> B and GO> B> Z respectively. In terms of kinetics aspect, it was observed that all adsorbents were comply with pseudo-second order reaction kinetics. To be positive of ΔG° value for B and Z is of physical nature of adsorption, to be negative of ΔG° value for GO requires to be chemical nature of adsorption. When the obtained ΔH° values were examined, it was observed that the reaction was endothermic for B and Z and exothermic for GO. The negative value of ΔS° for B and Z adsorbent species indicates that the randomness between solid and liquid decreases, whereas the positive ΔS° value for GO indicates that the randomness between solid and liquid increases. The highest adsorption capacity value obtained after the measurements made under optimum conditions is 589 mg g-1 for GO and 15.7 and 19.8 mg g-1 for B and Z, respectively.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro 茜草提取物对人类结直肠癌细胞株（HT-22）的影响：体外研究

Cumhuriyet Science Journal Pub Date : 2024-03-15 DOI: 10.17776/csj.1419826

S. Soylu

引用次数: 0

Evaluation of Antibiofilm and Antimicrobial Activities of N-heterocyclic Carbene Complexes 评估 N-杂环羰基复合物的抗生物膜和抗菌活性

Cumhuriyet Science Journal Pub Date : 2024-03-12 DOI: 10.17776/csj.1390256

Uğur Tutar, Cem Çelik, M. Atas

{"title":"Evaluation of Antibiofilm and Antimicrobial Activities of N-heterocyclic Carbene Complexes","authors":"Uğur Tutar, Cem Çelik, M. Atas","doi":"10.17776/csj.1390256","DOIUrl":"https://doi.org/10.17776/csj.1390256","url":null,"abstract":"In recent years, resistance to antimicrobials has become a global problem. Despite the need for new antibiotics with the increase of resistant bacteria, developing new antimicrobials is problematic. Biofilms formed by microorganisms play an essential role in the development of resistance. We aimed to investigate the antimicrobial and antibiofilm activities of N-heterocyclic carbene (NHC) complexes. In this study, previously synthesized and characterized NHC complexes on standard bacterial and fungal strains were investigated. The minimal inhibition concentration (MIC) test was used to determine the antimicrobial activities of the compounds, and the biofilm inhibition concentration test was used to determine the anti-biofilm activities. Compounds 2b and 2c showed potent antimicrobial activity on microorganisms between <=1.9 and 7.8µg/mL. Antimicrobial activity in salts of compounds (1a-1c) was weaker than silver compounds (2a-2c). The antibiofilm activity was between 27 and 79%, especially in silver-bound compounds (2a-2c). Benzimidazole derivative NHC compounds that we evaluated in our study were found to have significant antimicrobial and antibiofilm effects on pathogenic microorganisms. These compounds, which we assessed in our study, may be antimicrobial drug candidates that can be used in different areas. It will be essential to conduct further in vitro and in vivo studies on this subject.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aloe vera Gel Extract Prolongs Lifespan in Caenorhabditis elegans 芦荟凝胶提取物能延长秀丽隐杆线虫的寿命

Cumhuriyet Science Journal Pub Date : 2024-03-12 DOI: 10.17776/csj.1400264

Ş. Berk

引用次数: 0

Asymptotic Relative Efficiency Comparison for some Fit Indices in Structural Equation Modeling 结构方程模型中一些拟合指标的渐近相对效率比较

Cumhuriyet Science Journal Pub Date : 2024-03-12 DOI: 10.17776/csj.1328880

İlkay Doğan, İ. Doğan, N. Doğan

引用次数: 0

Electrospun Poly(ϵ-caprolactone) Nanofibers Containing Pomegranate Peel Extract and Bioactive Glass as Potential Wound Dressings 含有石榴皮提取物和生物活性玻璃的电纺聚（ϵ-己内酯）纳米纤维可用作潜在的伤口敷料

Cumhuriyet Science Journal Pub Date : 2024-03-12 DOI: 10.17776/csj.1383556

Ayşen Aktürk

{"title":"Electrospun Poly(ϵ-caprolactone) Nanofibers Containing Pomegranate Peel Extract and Bioactive Glass as Potential Wound Dressings","authors":"Ayşen Aktürk","doi":"10.17776/csj.1383556","DOIUrl":"https://doi.org/10.17776/csj.1383556","url":null,"abstract":"This study focuses on the effect of pomegranate peel extract (PPE) as a natural medicinal substance and 45S5 bioglass (BG) particles as a bioactive material on the microstructure, antioxidant properties, and fibroblast cell cytotoxicity of biocompatible poly(ε-caprolactone) (PCL) nanofiber scaffolds. The hybrid nanofibers were fabricated via the electrospinning technique. The microstructure of nanofiber scaffolds was characterized by using scanning electron microscopy (SEM). The results indicated that the incorporation of PPE and BG particles did not change the morphology of the fibrous structure of the PCL nanofiber scaffolds. The DPPH analysis was performed to determine the antioxidant properties of nanofiber scaffolds and demonstrated that the incorporation of PPE improves the antioxidant properties of scaffolds. Cell cytotoxicity studies using fibroblast L929 cells also showed that high cell viability values were observed for hybrid PPE and BG loaded PCL nanofiber scaffolds. The findings proved that the integration of PPE and BG particles into PCL nanofibers yielded favorable characteristics suitable for wound dressing purposes, involving improved antioxidant capacity.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"39 S181","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, Cytotoxic Activity Evaluation and Molecular Docking Studies of Some Benzimidazole Derivatives 一些苯并咪唑衍生物的合成、细胞毒性活性评估和分子对接研究

Cumhuriyet Science Journal Pub Date : 2024-03-12 DOI: 10.17776/csj.1392037

Aybüke Züleyha Kaya, Derya Osmaniye, Asaf Evrim Evren, Leyla Yurttaş, Ş. Demirayak

引用次数: 0

Synthesis of Gum Arabic-Based Biopolymer Network and Determination of Its Toxicity Properties in In Vitro - In Vivo Model Systems 合成阿拉伯胶基生物聚合物网络并在体外-体内模型系统中确定其毒性特性

Cumhuriyet Science Journal Pub Date : 2024-03-11 DOI: 10.17776/csj.1385443

İdil Karaca Açarı, Duygu Özhan Turhan, Ali Kuruçay, Süleyman Köytepe, B. Ateş

{"title":"Synthesis of Gum Arabic-Based Biopolymer Network and Determination of Its Toxicity Properties in In Vitro - In Vivo Model Systems","authors":"İdil Karaca Açarı, Duygu Özhan Turhan, Ali Kuruçay, Süleyman Köytepe, B. Ateş","doi":"10.17776/csj.1385443","DOIUrl":"https://doi.org/10.17776/csj.1385443","url":null,"abstract":"In this study, gum arabic based network polymers were prepared using epoxy functional PEG structures. The basic physicochemical properties of these structures, their structural characterization, thermal properties and morphological properties were investigated. Toxicity properties of constructs synthesized on zebrafish (Danio rerio (Hamilton)) offspring were determined in vivo. In addition, in vitro toxicity tests were performed on L929 fibroblast cells. When the general properties of these structures were examined. Structural and thermal properties were better with increasing cross-linker rates ratios (1%, 3%, 5%). According to the toxicity test performed on zebrafish juveniles; GA-PEG-Epox (1%) constructs are non-toxic to zebrafish juveniles. The mortality rate of GA-PEG-Epox (3%) and GA-PEG-Epox (5%) structures was observed as 12.5% and 20.8%, respectively. It was observed that the structures were not toxic to zebrafish juveniles. MTT test performed on L929 fibroblast cells, high cell viability (>90%) was observed in all synthesized structures. These results are evaluated as Grade 1 according to ISO standards.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"23 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140396555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0