Current Pharmaceutical Analysis最新文献

{"title":"UV-Visible Spectroscopy: A Review on its Pharmaceutical and Bio-allied Sciences Applications","authors":"Abhinav Singhal, Urvashi Saini, Bhawna Chopra, Ashwani K. Dhingra, Akash Jain, Jasmine Chaudhary","doi":"10.2174/0115734129300562240408042614","DOIUrl":"https://doi.org/10.2174/0115734129300562240408042614","url":null,"abstract":": Ultraviolet-Visible (UV-Vis) spectroscopy has emerged as a powerful analytical tool with diverse applications in pharmaceutical and bio-allied sciences. This article provides a comprehensive overview of the extensive utility of UV-Vis spectroscopy, emphasizing its pivotal role in characterizing and analyzing various compounds critical for drug development and bio-allied research. In the pharmaceutical sector, UV-Vis spectroscopy is a fundamental technique for quantifying the concentrations of active pharmaceutical ingredients (APIs) in formulations. Its non-destructive nature and high sensitivity make it an indispensable tool for quality control, ensuring the consistency and potency of pharmaceutical products. Furthermore, this technique has been employed in the study of drug-receptor interactions to elucidate the molecular mechanisms underlying therapeutic effects. In bio-allied applications, UV-Vis spectroscopy is used to analyze biomolecules like proteins, nucleic acids, and enzymes. This technique allows for the study of protein conformational changes, DNA structure, and enzymatic activity, offering crucial insights into fundamental biological processes. Additionally, UV-Vis spectroscopy aids in determining biomarker concentrations, assisting in the early diagnosis and monitoring of various diseases. This article also explores recent advancements in UV-Vis spectroscopy, including the integration of nanomaterials and computational approaches to enhance sensitivity and selectivity. Moreover, it discusses the potential of UV-Vis spectroscopy in emerging areas such as personalized medicine and point-of-care diagnostics. As technology continues to evolve, UV-Visible spectroscopy is poised to significantly contribute to the ever-expanding landscape of pharmaceutical and bio-related research.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"57 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Salvia cadmica on Fibroblast Cells from t-bhp-induced Oxidative Damage","authors":"Ceylan Hepokur, Sema Misir, Mehmet Cicek, Solomon Habtemariam, Javad Sharifi-Rad","doi":"10.2174/0115734129293569240327093703","DOIUrl":"https://doi.org/10.2174/0115734129293569240327093703","url":null,"abstract":"Background:: Salvia species known as \"Sage\" are among the important aromatic plants used in the world. This study, it was investigated the antioxidant capacity of Salvia cadmica and to investigate its protective effect on oxidative damage in t-BHP-induced fibroblast cells. Methods:: Antioxidant activity and phenolic characterization of the extract were evaluated using DPPH, TPC, TFC, FRAP, and HPLC, respectively.TAS, TOS, MDA and 8-oxo-guanine, CAT, SOD, and GPx values were examined using enzyme-linked immunosorbent assay (ELISA). The antiproliferative and apoptosis effects of Salvia cadmica ethanolic extract were determined using MTT assay and fluorescent probes in fibroblast cells. Results:: As a result of GC-MS analysis of Salvia cadmica ethanolic extract, carvacrol content was found to be high. The IC50 value of the DPPH antioxidant assay of the Salvia cadmica ethanolic extract was 80 ± 0.51 μg/mL. TPC, TFC, and FRAP values were found to be 18.25 ± 0.64 (mg gallic acid/g powder), 1.691±0.314 (mg quercetin /g powder), 31.5 ± 0.10 (mg Trolox/g powder), respectively. Total antioxidant and TOS values were found to be 0.383±0.033 (mmol Trolox Equ L-1), 16.31±0.71 (μmol H2O2 L-1) for 0.25 mg/mL, and 0.725±0.05 (mmol Trolox Equ L-1), 12.02 ±0.56 (μmol H2O2 L-1) for 0.5 mg/mL. In addition, while CAT and GPx significantly decreased enzyme activities, no significant change was observed in SOD enzyme activity. Ethanolic Salvia cadmica extract exhibited apoptotic features compared to only the t-BHP group. Conclusion:: These results suggest that Salvica cadmica extract works through a free radical mechanism.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"121 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the NF-κB Inhibition Peptides in Asthma from Pheretima aspergillum Decoction and Formula Granules Using Molecular Docking and Dynamics Simulations","authors":"Xiaotong Xiao, Yaxiong Liu, Yayang Huang, Wenjie Zeng, Zhuoya Luo","doi":"10.2174/0115734129298587240322073956","DOIUrl":"https://doi.org/10.2174/0115734129298587240322073956","url":null,"abstract":"Background: The Pheretima aspergillum decoction is a traditional therapeutic form, while the formula granules are produced through traditional Chinese medicine decoctions. However, the active ingredients in Pheretima aspergillum have not been fully elucidated, and no published reports have investigated the differences between Pheretima aspergillum decoction and formula granules. Objective: The study aimed to explore the potential bioactive peptides in Pheretima aspergillum decoction and formula granules and investigate their potential pharmacological mechanisms in alleviating inflammation associated with asthma through interaction with the IκBβ/NF-κB p65 complex. Methods: μLC-Q Exactive MS combined with de novo sequencing technology was employed to identify potential bioactive peptides in Pheretima aspergillum decoction and formula granules. Deep learning models were utilized to evaluate the bioactivity and toxicity of these peptides. Further investigations included molecular docking studies aimed at uncovering the interactions between the selected peptides and the IκBβ/NF-κB p65 complex at affinity and critical residue sites. Molecular dynamics simulations were conducted to assess the stability of the peptide-receptor complexes. Results: A total of 2,235 peptides from the Pheretima aspergillum decoction and 1,424 peptides from the Pheretima aspergillum formula granules were identified. Deep learning models resulted in the identification of 298 bioactive and non-toxic peptides from the decoction and 145 from the formula granules. Molecular docking revealed that 160 peptides from the decoction and 63 from the formula granules exhibited a strong affinity for the IκBβ/NF-κB p65 complex. The results of molecular dynamics simulations supported the stability of the interactions involving the peptide EGPANFADLGK from the decoction and the peptide KAAVDFGVPGDAGALAHLK from the formula granules with the IκBβ/NF-κB p65 complex. In conclusion, potential bioactive peptides were identified in both Pheretima aspergillum decoction and formula granules. Conclusion: This study has investigated the potential pharmacological mechanisms of peptides derived from Pheretima aspergillum decoction and formula granules in alleviating inflammation associated with asthma through the interaction of the IκBβ/NF-κB p65 complex, providing a basis for elucidating the molecular mechanism of action for the treatment of asthma.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"6 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing Hair for Drug Traces: A Review of Electrochemical Approaches","authors":"Jia Du, Li Fu, Xiaozhu Liu, Hassan Karimi-Maleh","doi":"10.2174/0115734129293976240320090141","DOIUrl":"https://doi.org/10.2174/0115734129293976240320090141","url":null,"abstract":"Background:: Hair analysis plays a crucial role in forensic toxicology, offering a unique medium for long-term drug trace detection. This review emphasizes the evolution of electrochemical methods in analyzing hair for drug traces, underscoring their significance in forensic science. Methods:: We examined recent advancements in electrochemical techniques, including voltammetry, amperometry, and electrochemical impedance spectroscopy, and their application in drug trace analysis. The review also explores the development of novel electrode materials and surface modifications, which enhance the detection capabilities of these methods. Results:: Electrochemical methods have shown high sensitivity and specificity in detecting a range of drugs in hair. Innovations, like molecularly imprinted polymers and nanomaterials, have expanded the detectable substance range, offering more refined and accurate detection. Despite challenges, such as hair variability and external contamination, these methods have significantly improved the reliability of drug trace analysis. Conclusion:: Electrochemical approaches to hair analysis represent a significant advancement in forensic toxicology. Their ability to provide sensitive, specific, and non-invasive analysis makes them valuable tools. Future developments, including portable device creation and integration with other analytical techniques, hold promise for further enhancing the scope and accuracy of drug trace detection in hair.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"5 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140313882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on Working Principle and Advanced Applications of Fluorescence activated Cell Sorting Machine (FACS)","authors":"Gurisha Garg, Preeti Patel, Ghanshyam Das Gupta, Balak Das Kurmi","doi":"10.2174/0115734129301463240306064929","DOIUrl":"https://doi.org/10.2174/0115734129301463240306064929","url":null,"abstract":": Various technologies, like flow cytometry and cell sorting, have been established in fields of biomedical research. Fluorescence-activated cell sorting is one of the most powerful techniques to witness advancement in these years. This article aims to provide an in-depth overview of the FACS applications, along with regulatory considerations and qualification parameters for the instrument. Moreover, specifications of instruments from different brands with specialized features are mentioned. FACS is a cornerstone in clinical diagnostics. This review highlights the current advancements and versatility of this indispensable technology, and the said information would be a focal paradigm for the upcoming biomedical and pharmaceutical research.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"20 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140153723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioselective Recognition of Chiral Α-Phenylethanol by Β-Cyclodextrin and Characterization of its Inclusion Behaviour Based On 2D ROESY","authors":"Jian Chen, Qiulin Li, Yawen Yang, Liang Qi, Xiang Han, Nan Zhang, Bin Zheng, Fuxin Chen","doi":"10.2174/0115734129285978240222103045","DOIUrl":"https://doi.org/10.2174/0115734129285978240222103045","url":null,"abstract":"Background:: α-phenylethanol and its derivatives are important intermediates for the synthesis of a variety of chiral drugs. Method:: The interaction mechanism of the two enantiomers of α-phenylethanol with β-CD was investigated using 1H-NMR and ROESY. The loading of β-cyclodextrin (β-CD) as the host with the chiral drug intermediate α-phenylethanol as the guest was investigated using high-resolution NMR in D2O and quantum chemical calculations. Results:: The results showed that both α-phenylethanol enantiomers were able to enter into the hydrophobic cavity of β-CD and undergo enantiospecific interactions, while the combination of 2D ROESY and quantum chemical calculations showed that the benzene ring of both R and S α- phenylethanol was inserted into the β-CD cavity toward the small-port end. However, the most stable poses of the two enantiomers were different, so the benzene ring of the S-enantiomer was oriented more toward the small-port end of β-CD than that of the R-enantiomer, which was reflected in the signals of 2D ROESY, which were obviously different. Conclusion:: β-CD can enantioselectively recognize the α-phenylethanol enantiomers, and the 2D ROESY method is a very direct and powerful tool in the recognition process of chiral host and guest research.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"101 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140115382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bionanofactories for the Environmental Friendly Fabrication of Silver Nanoparticles: Application to the Analysis of Antimicrobial Agents","authors":"Shatrudhan Prajapati, Shikha Yadav, Javed Khan","doi":"10.2174/0115734129281373240214071815","DOIUrl":"https://doi.org/10.2174/0115734129281373240214071815","url":null,"abstract":": The green synthesis of silver nanoparticles has garnered significant interest because of the unique physicochemical and biological properties they possess. These nanoparticles could have applications in a wide variety of fields, including biomedicine, cellular imaging, cosmetics, healthcare tourism, food and agriculture. The formation of nanoparticles is facilitated by the use of bionanofactories, or green synthesis processes, which utilise living organisms, biomolecules, and plant-based materials as bio reductive or bio sealing agents. Green chemistry is cost-effective in addition to being environmentally friendly, non-toxic, and biodegradable. By considering the results of recent studies using techniques like scanning electron microscopy, transmission electron microscopy, atomic force microscopy, ultraviolet/visible spectrophotometry, Fourier transform infrared spectroscopy or X-ray diffraction, we illuminate the most recent advances in green synthesis and the physicochemical properties of green silver nanoparticles. We also discuss the properties of silver nanoparticles that make them effective against bacteria, fungi, and parasites.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"51 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140055902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Quantitation of Non-chromophoric Vigabatrin and Gabapentin by a Validated qNMR Method in Bulk Drug and Marketed Formulations","authors":"Pooja Bedage, Archana Sahu, Inder Pal Singh","doi":"10.2174/0115734129283110240131044647","DOIUrl":"https://doi.org/10.2174/0115734129283110240131044647","url":null,"abstract":"Background:: Vigabatrin and gabapentin, commonly used antiepileptic drugs in clinics, lack a UV active chromophore and, therefore, require cumbersome derivatization methods for analysis by HPLC using fluorescence detection. This study demonstrated the use of NMR for their quantitative determination in pure form and their pharmaceutical formulations. Objective:: To develop a validated qNMR method for non-chromophoric drugs Vigabatrin and Gabapentin. Methods:: The signal of methine proton of vigabatrin at 3.67 ppm relative to the signal of maleic acid at 6.17 ppm and the methylene signal of gabapentin at 2.88 ppm relative to the signal of caffeine at 7.75 ppm was used for qNMR. The developed method was validated with respect to linearity, limits of detection and quantitation, accuracy, precision, specificity and solution state stability. Results:: Linearity range and r2 were found to be from 2.66 to 42.11 mg/mL and 0.9999. The limit of detection and quantification were 0.0129 mg/mL and 0.0391 mg/mL, respectively, for vigabatrin. This method was found to be linear (0.9998) and specific within the gabapentin concentration range from 1.07 to 34.24 mg/mL of D2O. The limits of detection and quantification were 0.0248 mg/mL and 0.0751 mg/mL, respectively. Conclusion:: Both methods were highly precise, with a calculated RSD of 0.60 % and 0.76 %, respectively. The robustness of the methods was revealed by changing pre and post-processing NMR parameters. The developed methods provide a simple and straight approach for the absolute determination of gabapentin and vigabatrin in bulk drugs and their marketed formulations without any pre-procedures.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"29 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139948663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Absorption Scanning and Assay of Serotonin Reception Inhibitor Antidepressant Drugs","authors":"Amanda Santana Silva, Victor Hugo Abreu de Paula, Lucas Gabriel dos Santos Xavier, Ludimila Nascimento da Silva, Ana Carolyne Silva Lucas, Aléxia Mariany Lourenço Alves, Fernando Machado dos Santos","doi":"10.2174/0115734129282354231226192406","DOIUrl":"https://doi.org/10.2174/0115734129282354231226192406","url":null,"abstract":"Background:: Spectrophotometric techniques are based on the absorption of electromagnetic energy by the molecules of the substance analyzed and can be applied to drug analysis. Objective:: This study has been proposed to carry out molecular absorption scanning and assay in the ultraviolet region of generic antidepressant drugs of the serotonin reuptake inhibitor class produced by three Brazilian laboratories, addressing the importance of good manufacturing practices, drug quality control, and the use of qualitative and quantitative analytical techniques. Methods:: The physicochemical analyses were performed in a Marte Científica (580 UVP) UV-Vis spectrophotometer, and the result was evaluated according to the acceptance interval described in the Brazilian Pharmacopoeia. Results:: When calculating the dosage of the fluoxetine hydrochloride sample, the value of 90.95% was obtained and, therefore, the drug was approved for the dosage test. The dosage test for the drugs, citalopram hydrobromide and sertraline hydrochloride, was also approved, obtaining values of 102.4% and 98.2%, respectively. Conclusion:: The results presented provide future perspectives regarding the rigor of the need for good drug handling practices, maintenance and qualification of quality control laboratory equipment, and the guarantee of validation of analytical methodologies used in the pharmaceutical industries, contributing to the improvement of the quality of production and pharmaceutical products analysis.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"50 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Evaluation of Banlangen Granule Based on Bioassays of Anti-influenzal and Anti-inflammatory Effects","authors":"Xiu-yu Qian, Ming-lu Zhang, Yan-Lin Wu, Shuang-cheng Ma, Jin-mei Liu, Yan-hui Kang, Li-xing Nie","doi":"10.2174/0115734129285820240108113029","DOIUrl":"https://doi.org/10.2174/0115734129285820240108113029","url":null,"abstract":"Objective:: In order to control the quality better, this study aimed to develop two bioassay methods of Banlangen Granule (BLGG) based on its anti-influenza activity and antiinflammatory activity and to verify the necessity of established methods by relating the results tested by chemical methods. Method:: First, the bioassay methods for determining the biopotency of the anti-influenza effect and anti-inflammatory activity were established and applied, taking neuraminidase and cyclooxygenase- 2 as disease targets, respectively. Secondly, the ultra-high-performance liquid chromatography coupled photo-diode array detector (UPLC-PDA) technique was used to perform fingerprints and quantify chemical compounds. Finally, the correlation analysis was performed on the results of bioassay methods and chemical methods to assist in choosing the effective quality markers for the BLGG. Results:: Two accurate, stable, and repeatable bioassay methods were developed and applied to the determination of 57 batches of samples. The chemical fingerprints and contents of seven quality compounds were obtained based on UPLC-PDA methods. From the results of correlation analysis, the highest intensity correlation between these quality markers was medium with a r=0.495 (P<0.01), which indicates the need for establishing a bioassay method for BLGG. Conclusion:: This present work illuminated that bioassay methods can be a great means to evaluate the quality of BLGG effectively and also provided a paradigm case for the quality control of other traditional Chinese medicine preparations.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"70 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139689853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}