Current Pharmaceutical Analysis最新文献

{"title":"Robust U-HPLC Method Development of Desonide and its Application to In Vitro Release Testing (IVRT) of Topical Cream Products","authors":"Seelam Jayadev, Ismail Y","doi":"10.2174/0115734129298659240606103013","DOIUrl":"https://doi.org/10.2174/0115734129298659240606103013","url":null,"abstract":"Background: The study focuses on establishing In Vitro Release Testing (IVRT) parameters for Desonide cream, following the guidelines of the Topical Classification System (TCS), to assess the bioequivalence between the Reference Listed Drug (RLD) and test. Aim: This study aimed to develop a reliable IVRT method using Franz diffusion cells. An environmentally friendly U-HPLC method was created to analyze Desonide in the samples. objective: This includes assessing Linearity, robustness, precision, sensitivity, reproducibility, selectivity, specificity, and recovery. In the context of In-vitro Release Testing (IVRT), the concept of accuracy becomes irrelevant because there isn't a definitive "actual" release rate. This is due to the variability in API release rates for a specific semisolid formulation under different test conditions Objective: To evaluate the drug release in Desonide products in accordance with SUPAC guidance, quantify the drug concentration using an analytical method, as per bioanalytical method validation guidelines, and ensure that the results meet the acceptance criteria. Linearity was established from 0.50μg/mL to 40μg/mL with acceptable regression values. Precision was confirmed three times, with an average % RSD of below 15% for 3 sets of 6QC level sample preparations. Stability tests demonstrated Desonide stability in receptor fluid (LLOQ and ULOQ) for 72 hours at 2-8°C and 25°C. Autosampler stability at LQC and HQC levels was proven at 25°C for 72 hours. Additionally, the stock solution remained stable at both 25°C and 2-8°C for 72 hours. Methods: The study involved evaluating the dosing regimen, release medium, and membrane while optimizing the U-HPLC method based on three variables including column temperature, mobile phase composition, and flow rate. After experimentation, it was determined that Nylon membrane and 0.9% NaCl: Methanol release media (70:30 v/v) with 1000 mg dose were used to maximize the release profile of desonide. Results: The created explanatory strategy is precise, delicate, and exact for measuring Desonide, with satisfactory Limits of Location LOD and Lower Limits of Measurement LLOQ measured at 0.15 and 0.50 ng /mL, respectively. The Regression coefficient r2 was identified to be 0.9996. The degree of Desonide measurement lessening was considered palatable, basically since the recuperation was underneath 30.00, additionally due to the favourable linear relationship watched within the Desonide discharge rates amid the IVRT study. Conclusion: All three generic products analyzed were found to be equivalent to the RLD, meeting for \"sameness\" outlined in the FDA's SUPAC-SS guidance. A novel U-HPLC method was developed for Desonide, covering the range from 0.5 to 40 μg/ml, with intra and inter-day variability below 2% RSD. Additional characterizations were established, and the stability of Desonide was successfully determined.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"55 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141588433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Review of Drugs Determined by Spectrophotometry using Vanillin as a Chromogenic Reagent in the Past Decade","authors":"Swathi Naraparaju, Barla Karuna Devi, Soujanya Chaganti, Pani Kumar D Anumolu, Sruthi sunkara","doi":"10.2174/0115734129306714240610070448","DOIUrl":"https://doi.org/10.2174/0115734129306714240610070448","url":null,"abstract":": Many analytical techniques have been used in quality control, such as spectrophotometry, spectrofluorimetry, HPLC, and other hyphenated techniques. Among them, spectrophotometry is considered to be one of the most commonly used simple techniques. Drugs that lack chromogenic groups can be readily determined by using the chromogenic reagents, which react with the functional groups present in the drugs and produce a chromogenic group that can be detected in the visible region using a spectrophotometer. Chromogenic reagents play a vital role in the estimation of such types of drugs. Vanillin is one of the chromogenic reagents that possess a carbonyl group that reacts with the drugs that possess amine moiety and results in the formation of Schiff’s base, which is a yellow-colored compound that can be detected by spectrophotometry. The present review gives insights into the reaction conditions and applications of the drugs that are estimated by using vanillin as a chromogenic label.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"51 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronized Assessment of Lobeglitazone Sulfate and Metformin Hydrochloride in Tablet by Robust, High-performance Thin-layer Chromatographic Method","authors":"Dhanya B. Sen, Krunal Baldha, Ashim Kumar Sen, Rajesh A. Maheshwari, Aarti S. Zanwar, K. P. Greeshma, Prasanna Kumar Pradhan","doi":"10.2174/0115734129303749240607083542","DOIUrl":"https://doi.org/10.2174/0115734129303749240607083542","url":null,"abstract":"Background: A combination of fixed-doses containing 0.5 mg lobeglitazone sulfate and 1000 mg metformin hydrochloride has demonstrated efficacy in enhancing glycemic control in diabetes. Aim: The projected work aimed to establish and validate a high-performance thin-layer chromatographic methodology for the quantification of both drugs in tablet formulations. Objective: The task involves creating and validating a method in accordance with ICH guidelines to quantify two particular drugs in tablet formulations accurately. Methods: The high-performance thin-layer chromatographic analysis utilized aluminum plates layered with silica gel 60F254, and the solvent system consisted of acetonitrile, 1 M ammonium acetate (methanol), toluene, and triethyl amine (1.5:2.5:4:0.2 v/v/v/v), followed by densitometric scanning at 237 nm. Results: The methodology exhibited linearity in the range of 100-1500 ng/band for lobeglitazone sulfate and 1000-15000 ng/band for metformin hydrochloride, with correlation coefficients of 0.9991 and 0.9992, correspondingly. Exceptional sensitivity was observed, with detection limits of 8.17 ng/band for lobeglitazone sulfate and 271.34 ng/band for metformin hydrochloride, along with quantification limits of 24.75 ng/band for lobeglitazone sulfate and 822.24 ng/band for metformin hydrochloride. The method demonstrated precision (% relative standard deviation of peak area <360;2) and accuracy (recovery between 96 and 103%). Conclusion: The suggested methodology is fit for the concurrent quantification of both drugs in tablet formulations, making it applicable for routine quality control assessments in laboratories.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"95 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy Coupled with Multivariate Classification Chemometric Model for Routine Screening of Paracetamol, Ibuprofen, and Aspirin Adulteration in Herbal Products","authors":"Mario Theodore, Vorasit Vongsutilers","doi":"10.2174/0115734129295505240430092112","DOIUrl":"https://doi.org/10.2174/0115734129295505240430092112","url":null,"abstract":"Objective: The objective of this study is to develop and validate a routine screening test for the determination of three common antipyretic-analgesic synthetic drugs (paracetamol, ibuprofen, and aspirin) adulteration in herbal products using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) coupled with chemometric method. Method: ATR-FTIR spectra of sixteen testing sets of herbal product samples for pain and fever indications were used for multivariate chemometrics model construction. Linear Discriminant Analysis (LDA) was selected as a method for model construction with IBM SPSS for statistical analysis. Model development employed feature selection, such as the stepwise method for variable selection. The model with a high %correct classification and cross-validation was selected and was then validated with an independent testing data set with an auto-prediction test, confusion matrix, and Receiver Operating Characteristic (ROC) curve. To validate the developed test for routine use, the result from ATR-FTIR method was compared with the standard HPLC and TLC analyses used for adulteration screening. objective: Creating validated screening tools for herbal products adulterated with three common antipyretic-analgesic synthetic drugs (Paracetamol, Ibuprofen, and Aspirin) using ATR-FTIR couple with chemometric method Results: The selected model's overall %correct classification result was 97.7%, with a cross-validation of 93.8% rate in training set samples. External validation with an independent testing dataset gave an overall correct classification of 93.8%, with an area under the curve of ROC at 0.979. Comparative testing revealed that model performance was comparable with the HPLC and TLC methods, which routinely detect the presence of paracetamol, aspirin, and ibuprofen. The results of testing set samples classification were consistent with training set samples. Conclusion: Against the standard chromatographic methods, the multivariate chemometric model based on ATR-FTIR demonstrates comparable detection capability to determine adulteration of paracetamol, ibuprofen, and aspirin in herbal products.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"16 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140885047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application and Development of Targeted Fishing Technology in Natural Product Screening - A Simple Minireview","authors":"Yingying Su, Weiping Wang, Ying Wang, Chen Wang, Shuai Sun, Xianhong Zhu, Xiao Dai, Shiyu Li, Xun Gao, Kunming Qin","doi":"10.2174/0115734129301241240429114323","DOIUrl":"https://doi.org/10.2174/0115734129301241240429114323","url":null,"abstract":"Background: The screening of active ingredients in traditional Chinese medicine is an important task in the modernization of traditional Chinese medicine, and the commonly used analytical means in the past were mainly to screen the extracts of traditional Chinese medicine through pharmacological experiments, but the method has major defects. The target fishing strategy provides a new idea for the screening of active ingredients, and it has rapidly become a hot research direction, but there are many methods that need to be summarized and aggregated. Objective: It aims to provide readers with an understanding of the achievements, developments, and dilemmas of target fishing techniques over the past few years and to provide new ideas for subsequent research. Methods: Research articles in recent years using target fishing as an entry point are used as a basis to summarize the types of literature based on their principles and characteristics and to discuss the advantages and disadvantages of each method. Conclusion: This paper summarizes the classification and development of fishing techniques such as ultrafiltration, equilibrium dialysis, cell membrane chromatography, and immobilization of target molecules and target fishing and describes the principles and characteristics of these methods. The applications of these methods in the active ingredients of traditional Chinese medicine are summarized, and the problems and solutions of these methods are discussed.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"12 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Trends in the Detection of Alkaloids through Analytical, Bioanalytical, and Electrochemical Techniques : Analytical Techniques Used in Detection of Alkaloids","authors":"Bharat Sharma, Rohit Bhatia, Subrahmanya S. Ganti, Naresh Kumar Rangra","doi":"10.2174/0115734129307329240430071035","DOIUrl":"https://doi.org/10.2174/0115734129307329240430071035","url":null,"abstract":": Alkaloids provide significant health benefits in moderation, but excessive levels can pose health hazards. They play an important role in the creation of numerous pharmacological medications, acting as potent antispasmodics, analgesics, and even anti-cancer medicines. A detailed review of sensitive and accurate alkaloid analytical techniques can be used as a guide for future analyses of alkaloids in pertinent research. The main aim of this manuscript is to review the literature on the detection and separation of alkaloids by using various methods like analytical, bioanalytical, and electrochemical techniques, published during 2018-2023. An in-depth review of the literature was carried out using a variety of databases, including Web of Knowledge, PubMed, and Google Scholar. Consulting relevant published materials, including books, was another aspect of this research. The keywords used in the search were alkaloids, analytical techniques, bio-analytical techniques, electrochemical techniques, and biosensors. These were carefully examined in more detail, and significant data and findings were collected and presented using tables. The publication highlights the significance of advanced chromatographic techniques in the separation and isolation of alkaloids. It discusses several analytical, bio-analytical, and electrochemical analytical techniques, which include sensors and biosensors, and adds to the extensive review of alkaloid detection techniques. Recent advancements and methodologies for improving the knowledge of the detection and separation of alkaloids are presented in this article, which is beneficial for the researcher involved in developing analytical methods for alkaloid detection. Current efforts and advanced analytical approaches for alkaloid detection are given in this manuscript, which is crucial in favor of improving the health and wellness of society.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"25 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Diosmin on Pharmacokinetics and Pharmacodynamics of Rivaroxaban in Rats","authors":"Siwen Wang, Mingyu Cui, Fan Wu, Chao Yu, Yue Sui, Xueying Yan, Yingli Gai","doi":"10.2174/0115734129282400240417115747","DOIUrl":"https://doi.org/10.2174/0115734129282400240417115747","url":null,"abstract":"Background and Objective: Rivaroxaban, a direct oral anticoagulant, has become the first-line therapy medicine to prevent and treat Venous Thromboembolism (VTE). Patients with femoropopliteal venous thrombosis may use rivaroxaban along with diosmin. Rivaroxaban is the substrate of CYP3A4 and P-glycoprotein (P-gp), but diosmin is the inhibitor. The combination might lead to Drug-drug Interaction (DDI). The aim of this study was to assess the effect of diosmin on the pharmacokinetics and pharmacodynamics of rivaroxaban in rats. Methods: Plasma concentration of rivaroxaban in the absence or presence of diosmin groups was determined by High-performance Liquid Chromatography (HPLC). Pharmacokinetics parameters were calculated and used to evaluate pharmacokinetics interactions. Anticoagulation was investigated by Prothrombin Time (PT), International Normalized Ratio (INR), and Activated Partial Thromboplastin Time (APTT). Antithrombotic efficacy was investigated by the length of tail thrombosis, the content levels of Interleukin-1β (IL-1β) and D-dimer (D-D) in rats, and histopathological sections in the tail thrombosis model. Results: Maximum concentration (Cmax), 0-t Area Under the Curve (AUC0–t), 0-∞ Area Under the Curve (AUC0–∞) of rivaroxaban increased significantly in the combination group. PT, INR, and APPT in the combination group exhibited an increase compared to the Rivaroxaban group. Simultaneously, the length of tail thrombosis and levels of IL-1β and D-D were significantly reduced. Significant improvement of tissue histology in tail thrombosis could be observed. Conclusion: Taken together, diosmin could significantly affect the pharmacokinetics and pharmacodynamics of rivaroxaban, and enhance anticoagulant and antithrombotic efficacy in rats. More attention should be paid to avoid harmful DDI in the clinic.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"87 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140838907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality by Design-driven Analytical Method: A Quality Risk Management-Based Liquid Chromatography Method for Daclatasvir and Characterization of its Putative Degradants by LC-MS/MS","authors":"Prashant Chaturvedi, Shruti Chopra, Kalyani Joshi, Savita Tauro","doi":"10.2174/0115734129285465240408044841","DOIUrl":"https://doi.org/10.2174/0115734129285465240408044841","url":null,"abstract":"Background:: Antiviral drugs can cure more than 95 percent of people with hepatitis C, but the inaccessibility of quality affordable medicines and the lack of their uninterrupted supply pose a major challenge. Impurities in drugs have a significant impact on their quality and are one of the substantial causes of drug recalls, ultimately leading to the unavailability of the drug in the market. Hence, there is a need for a robust, quality risk management and quality by design-driven analytical method that can detect the antiviral drug, Daclatasvir dihydrochloride, in the presence of its probable impurities. Objective:: This study aimed to develop a Quality by Design-driven stability- indicating liquid chromatography method for Daclatasvir dihydrochloride and the characterization of its putative degradants by LC-MS. Method:: The fishbone diagram and quality risk assessment investigated twenty-four process parameters and concluded that three risk parameters, i.e., flow rate, buffer pH, and stationary phase type, were the critical process parameters. The critical quality attributes viz. resolution between impurity 6 and DCV and impurity 2 & 3 (Rs˃1.5), the shape of the peak of DCV which is decided by the Number of Theoretical Plates (NTP˃5000), and the retention time of Daclatasvir (tR14-23 mins) were optimized using a two-level three-factor full factorial design with five center points. Results:: The optimized method is stability-indicating in its true sense as it can separate the sample with its degradants generated in basic (three), acidic (two), oxidative (H2O2: three, Azobisisobutyronitrile: one), photo (three), and dry heat (one) conditions. Degradants structures were elucidated, and degradation routes were established, using LC-MS and LC-MS/MS analyses. Conclusion:: The drug is highly susceptible to acid, base hydrolysis, and oxidation degradation conditions and poses a significant risk to the analytical method to fail in system suitability criteria. Hence, a robust and flexible chromatographic method with the capacity for continuous improvement was developed and successfully validated within the criteria of design space.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"60 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140838757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Development and Validation of Atoltivimab, Maftivimab and Odesivimab in Pharmaceutical Dosage form by using the RP-HPLC Method","authors":"Pallepogu Venkateswara Rao, Naidu Srinivasa Rao, Biswa Mohan Sahoo, Nayaka Raghavendra Babu","doi":"10.2174/0115734129300296240416070559","DOIUrl":"https://doi.org/10.2174/0115734129300296240416070559","url":null,"abstract":"Background: The reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed for the quantitative measurement of monoclonal antibodies (Maftivimab, Atoltivimab, and Odesivimab) in the pharmaceutical dosage form. The Food and Drug Administration (FDA) has approved these monoclonal antibodies for the treatment of Zaire ebolavirus infection in adults. Methods: Maftivimab, Atoltivimab, and Odesivimab were separated chromatographically on the Waters Alliance-e2695 platform using the Luna Phenyl Hexyl (250 x 4.6 mm, 5 μm) column and a mobile phase made up of Acetonitrile (ACN) and ortho-phosphoric acid (OPA) buffer in a ratio of 70:30 (v/v). Results: The flow rate was 1.0 ml/min, and a photodiode array (PDA) detector operating at room temperature was used to measure absorption at 282 nm. For Maftivimab, Atoltivimab, and Odesivimab, the theoretical plates were not less than 2000, and the tailing factor shouldn't be greater than 2, accordingly. All measurements have a constant relative standard deviation of peak areas that is less than 2.0. Conclusion: The suggested procedure was approved following the International Conference on Harmonisation (ICH) recommendations. When used for the quantitative analysis of Maftivimab, Atoltivimab, and Odesivimab, the approach was found to be straightforward, affordable, appropriate, exact, accurate, and robust.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"106 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140811612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Ketamine and Palmatine in Rat Plasma by UHPLC-MS/ MS and their Pharmacokinetic Interaction","authors":"Xueli Huang, Yizhe Ma, Ziyue Wang, Wanhang Wang, Congcong Wen, Xianqin Wang, Meiling Zhang","doi":"10.2174/0115734129304769240403075839","DOIUrl":"https://doi.org/10.2174/0115734129304769240403075839","url":null,"abstract":"Objective:: Ketamine, commonly known as “K-powder,” is increasingly being abused as a “prom drug.” Palmatine, a typical isoquinoline alkaloid, is mainly found in the roots and stems of natural Chinese herbal medicine plants such as Phellodendron chinense, Coptis chinensis, Sankezhen and Nantianzhu. Herein, we aim to establish a UHPLC-MS/MS method to determine ketamine and palmatine concentrations in rat plasma and investigate the pharmacokinetic interaction of ketamine and palmatine. Methods:: Three groups of eighteen rats each were assigned to ketamine, palmatine, ketamine and palmatine. The pharmacokinetic interaction between ketamine and palmatine was demonstrated using UHPLC-MS/MS. Results:: When ketamine was combined with palmatine, the mean residence time (MRT) was significantly different from that of the ketamine group. MRT decreased after combined use. The interaction showed that palmatine can influence the mean residence time of ketamine; no significant differences were observed for other pharmacokinetic parameters between the ketamine or palmatine group and the ketamine-palmatine group. Conclusion:: Palmatine may influence the mean residence time of ketamine.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"207 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}