Crop Science最新文献_第9页

Optimized nitrogen application rate based on soil residual nitrogen significantly increased the yield and biological nitrogen fixation of fresh faba bean as vegetables 以土壤残氮为基础的优化施氮量显著提高了新鲜蔬菜蚕豆的产量和生物固氮能力

IF 2 3区农林科学

Crop Science Pub Date : 2025-03-06 DOI: 10.1002/csc2.70013

Yi Pu, Kuai Dai, Jiangzhou Li, Yan Wang, Shan Lin, Meiju Liu

{"title":"Optimized nitrogen application rate based on soil residual nitrogen significantly increased the yield and biological nitrogen fixation of fresh faba bean as vegetables","authors":"Yi Pu, Kuai Dai, Jiangzhou Li, Yan Wang, Shan Lin, Meiju Liu","doi":"10.1002/csc2.70013","DOIUrl":"https://doi.org/10.1002/csc2.70013","url":null,"abstract":"Optimizing nitrogen (N) application rate during faba bean (Vicia faba (L.) Linné) growth season might increase biological N fixation (BNF) and decrease soil residual N which will be benefit to the growth of following N sensitive crops. A 4-year field study was conducted with six N rates (0, 45, 90, 135, 180, and 225 kg N ha−1). Yield, agronomic traits, root and nodule characteristics, N uptake, and biological N fixation were measured. Our results showed that (1) pod and grain yield, nodule weight and count, and BNF of faba bean initially increased with N rates, peaking at 90 kg N ha−1, and then declined. (2) BNF measured by 15N natural abundance strongly correlated with 15N isotope dilution method. The maximum BNF of 61 kg N ha−1 occurred at the N rate of 90 kg N ha−1, which was significantly greater than that at the other N rates. (3) Root nodule dry weight and count were positively correlated with BNF, as were shoot dry weight and pod yield. (4) The N equation, which is based on the sum of N rate plus soil residual N before sowing, revealed that the pod yield and BNF peak when the total N is <200 kg N ha−1. In conclusion, to improve the yield and quality of the next N-sensitive crops and reduce environmental pollution risk, N rate for faba bean should be significantly reduced and adjusted on the basis of soil residual N before sowing. This maximizes the BNF potential of faba bean.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seedling emergence in winter and spring canola genotypes under salinity stress 盐胁迫下冬、春油菜基因型的出苗研究

IF 2 3区农林科学

Crop Science Pub Date : 2025-03-06 DOI: 10.1002/csc2.70011

Rajan Shrestha, Qingwu Xue, Andrea Leiva Soto, Girisha Ganjegunte, Santosh Subhash Palmate, V. N. Chaganti, S. Kumar, A. L. Ulery, R. P. Flynn, S. Zapata

{"title":"Seedling emergence in winter and spring canola genotypes under salinity stress","authors":"Rajan Shrestha, Qingwu Xue, Andrea Leiva Soto, Girisha Ganjegunte, Santosh Subhash Palmate, V. N. Chaganti, S. Kumar, A. L. Ulery, R. P. Flynn, S. Zapata","doi":"10.1002/csc2.70011","DOIUrl":"https://doi.org/10.1002/csc2.70011","url":null,"abstract":"In the worldwide context of rising salinity issues in agriculture, it is important to understand crop responses to salinity stress. Currently, standing as the second largest oilseed crop, canola (Brassica napus L.) entices continued research focus on such aspects. Thus, this study investigated the genotypic variation in seedling emergence characters under salinity stress. Two growth chamber experiments were conducted in diverse canola genotypes (10 each of winter and spring types) at six salinity levels (0, 2, 4, 6, 8, and 10 dS m−1 EC). Increasing salinity levels reduced the emergence indices (emergence percentage, emergence rate index, corrected emergence rate index, and emergence velocity) and salt tolerance index (STI). An approximate threshold salinity range of 6–8 dS m−1 ECs was determined. Importantly, salinity at ≥8 dS m−1 EC levels substantially reduced seedling emergence indices and delayed emergence by 3–7 days after seeding. Winter genotypes CP1022WC/Chinook and CP320WRR, and spring genotypes Monarch, PI597352, PI601200, and PI432395 had higher STI and emergence indices. Based on cluster analysis, genotype groups were classified as low (Athena, CP115W, Durola, Impress, and Gem), medium (Amanda, Ericka, CP320WRR, Salut, CP225WRR, Clearwater, and Wester), and high salt-tolerant types (CP1022WC/Chinook, Monarch, PI597352, and PI432395). All emergence indices showed high broad-sense heritability (H2 = 0.82–0.94). Between canola types, spring canola consistently showed greater genetic potential for salt tolerance than winter canola. The results of this study provided useful information for canola seedling establishment under salinity and for further genetic improvement of salt tolerance.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epichlöe festucae endophyte mediated maternal inheritance of dollar spot disease resistance in hard fescue Epichlöe羊茅内生菌介导的硬羊茅抗斑病母系遗传

IF 2 3区农林科学

Crop Science Pub Date : 2025-03-06 DOI: 10.1002/csc2.70012

Trent M. Tate, Ruying Wang, Stacy A. Bonos, Bruce B. Clarke, Alec R. Kowalewski, William A. Meyer

{"title":"Epichlöe festucae endophyte mediated maternal inheritance of dollar spot disease resistance in hard fescue","authors":"Trent M. Tate, Ruying Wang, Stacy A. Bonos, Bruce B. Clarke, Alec R. Kowalewski, William A. Meyer","doi":"10.1002/csc2.70012","DOIUrl":"https://doi.org/10.1002/csc2.70012","url":null,"abstract":"Hard fescue (Festuca brevipila) is a fine-leaved cool-season turfgrass and is well adapted for low-maintenance areas, such as home lawns, parks, and roadsides. Breeding for improved disease resistance is a major objective in utilizing hard fescue under low-input management. The Epichlöe festucae endophyte-mediated dollar spot (Clarireedia jacksonii) resistance and its mechanism have been well-documented in strong creeping red fescue (F. rubra subsp. rubra). However, little is known about dollar spot resistance in hard fescue. The objectives of this research were to (1) understand the inheritance of dollar spot resistance in hard fescue and determine the reciprocal effects by performing a diallel cross between three resistant endophyte-containing and three susceptible endophyte-free parents and (2) confirm the role of E. festucae endophyte in dollar spot resistance by artificially inoculating hard fescue with the endophyte (ME+) and comparing ME+ plants to their genetically identical counterparts without the endophyte (E‒) under dollar spot pressure. The highly significant maternal effect from the diallel cross experiment demonstrated that the dollar disease resistance is maternally inherited in hard fescue and linked with the maternal inheritance of the E. festucae endophyte. The greenhouse study further confirmed that the presence of E. festucae endophyte significantly reduced dollar spot in hard fescue. Understanding this endophyte effect on maternal inheritance of dollar spot resistance will enable more efficient breeding and selection of plant materials to use in the development of improved dollar spot resistant cultivars in hard fescue.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a greenbug resistance gene in a wheat breeding line BW 49921 小麦育种品系bw49921抗绿虫基因的鉴定

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-21 DOI: 10.1002/csc2.70019

Genqiao Li, Tezera W. Wolabu, Guihua Bai, Ruolin Bian, Amy Bernardo, Brett F. Carver, Yanqi Wu, Xiangyang Xu

{"title":"Characterization of a greenbug resistance gene in a wheat breeding line BW 49921","authors":"Genqiao Li, Tezera W. Wolabu, Guihua Bai, Ruolin Bian, Amy Bernardo, Brett F. Carver, Yanqi Wu, Xiangyang Xu","doi":"10.1002/csc2.70019","DOIUrl":"https://doi.org/10.1002/csc2.70019","url":null,"abstract":"Greenbug [Schizaphis graminum (Rondani)] is a major wheat (Triticum aestivum L.) pest that causes significant yield losses worldwide. Use of plant resistance is an effective and environmentally friendly strategy to manage greenbug. BW 49921 is a spring wheat breeding line showing resistance to major US greenbug biotypes. Genetic analysis of an F2:3 population from the cross BW 49921 × Breakthrough revealed a single dominant gene conditioning greenbug resistance in BW 49921, and selective genotyping identified a genomic region associated with the greenbug resistance in chromosome arm 7DL. Ten single nucleotide polymorphisms (SNPs) in the target genomic region were converted to kompetitive allele-specific PCR (KASP) markers to genotype the entire F2 population. Linkage analysis using F2:3 phenotypic data delimited the greenbug resistance gene in BW 49921, designated Gb49921, to a 0.5-Mb interval between Stars-KASP419 (598.8 Mb) and Stars-KASP1061 (599.3 Mb). Gb49921 was 1.0 cM distal to Stars-KASP419 and 0.6 cM proximal to Stars-KASP1061. Allelism tests indicated that Gb49921 is not allelic to either Gb3 or Gb8. Gb49921 confers high resistance to greenbug biotypes C, E, H, I, and K and moderate resistance to TX1. The KASP markers developed in this study can be used for marker-assisted selection of Gb49921 in wheat breeding programs.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yield variation, plasticity, adaptation, and performance ranking of winter wheat varieties across the environmental gradient of the US Pacific Northwest 美国太平洋西北部不同环境梯度冬小麦品种的产量变化、可塑性、适应性和性能排名

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-21 DOI: 10.1002/csc2.70018

Curtis B. Adams, Clark Neely, Ryan Graebner

{"title":"Yield variation, plasticity, adaptation, and performance ranking of winter wheat varieties across the environmental gradient of the US Pacific Northwest","authors":"Curtis B. Adams, Clark Neely, Ryan Graebner","doi":"10.1002/csc2.70018","DOIUrl":"https://doi.org/10.1002/csc2.70018","url":null,"abstract":"The US Pacific Northwest is a major wheat (Triticum aestivum L.) production region with widely ranging water availability across its dryland and irrigated cropping systems. There have been no evaluations of variation, plasticity, adaptation, and performance of wheat yield due to variety—collectively or individually—that consider the entire environmental gradient of the region, useful for yield gap analysis, informing breeding, and other applications. Our objectives were to provide these evaluations using a large variety trial dataset and an independent dataset. Using linear models covering site-averaged yields (× variable) from about 1.50 to 12.0 Mg ha−1, upper and lower yield boundaries due to differences in variety performance were characterized by the regression models y = 1.08x + 0.24 and y = 0.906x − 0.13, respectively. The accuracy and usefulness of these functions were validated by the close fit of an independent dataset. Yield plasticity and environmental adaptation of 45 individual wheat varieties were also evaluated using the Finlay–Wilkinson regression approach, which showed wide variation in varietal yield responses to environment and substantial differences in yield stability (i.e., variation around the yield trend). A limitation of this analytical approach has been that the regression coefficients are not directly useful for ranking varieties for yield performance across contrasting environments. A simple transformation procedure of the coefficients into a single metric was effective for rapidly providing such rankings. A limited analysis is presented quantifying the relative contributions of genotype (27%–58% within sites), environment (84% across sites), and their interaction (5% across sites) on regional yields, as well as yield analysis of club wheat. In summary, this work provides useful research tools and a region-wide perspective on yield traits of Pacific Northwest wheat varieties.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of QTLs for reproductive stage salinity tolerance in rice using a cross between CSR28 and BRRI dhan28 利用CSR28与BRRI dhan28杂交鉴定水稻生殖期耐盐性qtl

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-21 DOI: 10.1002/csc2.70014

Sejuti Mondal, Robert Vaughn, Endang M. Septiningsih, Rakesh K. Singh, Michael J. Thomson

{"title":"Identification of QTLs for reproductive stage salinity tolerance in rice using a cross between CSR28 and BRRI dhan28","authors":"Sejuti Mondal, Robert Vaughn, Endang M. Septiningsih, Rakesh K. Singh, Michael J. Thomson","doi":"10.1002/csc2.70014","DOIUrl":"https://doi.org/10.1002/csc2.70014","url":null,"abstract":"Salinity poses a serious threat to rice (Oryza sativa L.) production in coastal regions across the globe. Since salinity tolerance is a complex and polygenic trait, understanding the salt-tolerance mechanisms is key for rice breeding. Breeding efforts can be aided by quantitative trait locus (QTL) mapping for complementary salt tolerance mechanisms in plants. While numerous studies are available on salinity tolerance at the seedling stage, limited studies have been conducted on reproductive stage tolerance in rice due to the difficulty in achieving reliable stage-specific phenotyping techniques. In this study, a BC1F2 mapping population consisting of 435 individuals derived from a cross between a salt-tolerant Indian variety, CSR28, and a salt-sensitive Bangladeshi mega variety, BRRI dhan28, was evaluated for yield components after exposure to salinity stress of EC 10 dS/m during the reproductive stage. After selecting extremely tolerant and sensitive progenies, 190 individuals were genotyped by kompetitive allele-specific PCR (KASP) genotyping with 152 high-quality single nucleotide polymorphic (SNP) markers. Consequently, 15 QTLs were identified under reproductive stage salt stress, including plant height, panicle length, number of filled and unfilled spikelets, percent filled spikelets, grain yield, and the Na+-K+ ratio. Notably, three QTLs, one each for the number of filled spikelets (qNFS10.1), percent filled spikelets (qPFS10.1), and grain yield (qGY10.1), were mapped at the same position (75.9 cM) on chromosome 10. These promising QTLs can be examined further to dissect the molecular mechanisms underlying reproductive stage salinity tolerance in rice.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genetic loci associated with protein and fiber digestibility in alfalfa (Medicago sativa L.) 紫花苜蓿蛋白质和纤维消化率相关基因位点的鉴定

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-18 DOI: 10.1002/csc2.70004

Sen Lin, Cesar Augusto Medina, Smit Bipinchandra Patel, Zhanyou Xu, Geoffrey I. Zanton, David Combs, Guojie Wang, Glenn Shewmaker, Steve Fransen, Don Llewellyn, Steven Norberg, Long-Xi Yu

{"title":"Identification of genetic loci associated with protein and fiber digestibility in alfalfa (Medicago sativa L.)","authors":"Sen Lin, Cesar Augusto Medina, Smit Bipinchandra Patel, Zhanyou Xu, Geoffrey I. Zanton, David Combs, Guojie Wang, Glenn Shewmaker, Steve Fransen, Don Llewellyn, Steven Norberg, Long-Xi Yu","doi":"10.1002/csc2.70004","DOIUrl":"https://doi.org/10.1002/csc2.70004","url":null,"abstract":"Alfalfa (Medicago sativa L.), known as the queen of forages, is a versatile and valuable forage crop that holds significant importance in agriculture due to its myriad benefits for livestock. Ruminants benefit from alfalfa's digestible fiber and protein, contributing to improved feed efficiency and milk production. However, alfalfa protein is rapidly and extensively degraded in rumen, and it is a challenge to maximize the efficiency of the forage crude protein utilized as metabolizable protein by ruminant livestock. In this study, the phenotypic data of 14 traits related to forage digestibility were collected from 200 alfalfa accessions planted at three different locations for 2 years. The performance of these accessions showed dramatic variations by location, indicating that environmental factors play important roles in alfalfa digestibility. Twenty-two significant genetic markers associated with 12 traits related to forage digestibility were identified by genome-wide association study. Among them, seven markers were associated with more than one trait, although the significant markers varied by year and location. Putative candidate genes associated with these loci were also identified. The digestibility-related markers and associated genes identified in this study will help to better understand the genetic basis of forage digestibility and its interaction with environments. After validation, the closely linked markers and associated genes can be used for marker-assisted selection of alfalfa with improved forage quality.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A crop wild relative inventory for Brazil 巴西作物野生相关库存

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-17 DOI: 10.1002/csc2.70001

Monique Moreira Moulin, Talles de Oliveira Santos, Semiramis Rabelo Ramalho Ramos, Nigel Maxted, Joana Magos Brehm

{"title":"A crop wild relative inventory for Brazil","authors":"Monique Moreira Moulin, Talles de Oliveira Santos, Semiramis Rabelo Ramalho Ramos, Nigel Maxted, Joana Magos Brehm","doi":"10.1002/csc2.70001","DOIUrl":"https://doi.org/10.1002/csc2.70001","url":null,"abstract":"Crop wild relatives (CWRs) are defined as wild plant taxa that are closely related to agricultural crops. In the face of global pressures of climates change and biodiversity loss, CWR requires urgent conservation attention. As a first step toward a national strategy for the conservation of CWRs, we present an inventory of taxa occurring in Brazil, with suggested prioritization of species based on potential value in crop improvement. The CWR checklist comprised 3652 taxa among 112 genera, and after applying the prioritization criteria, 289 CWRs were prioritized. The prioritized CWRs are related to crops of regional, national, and global importance such as cassava (Manihot esculenta Crantz), sugarcane (Saccharum officinarum L.), coffee (Coffea arabica L. and Coffea canephora Pierre ex A. Froehner), cotton (Gossypium hirsutum L.), rice (Oryza sativa L.), orange (Citrus spp.), beans (Phaseolus vulgaris L.), tomato (Solanum lycopersicum L.), potato (Solanum tuberosum L.), and cocoa (Theobroma cacao L.). More than half of the prioritized CWRs (50.86%) are endemic, and 28.03% are nationally endangered. A subsequent prioritization was also carried out using the criteria of socioeconomic value of the related crop and endemism, with 95 taxa being prioritized. The production of the first CWR inventory for Brazil is an important tool for planning conservation actions as part of a national strategy to maintain food security.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-resolution genome-wide association study reveals two genes influencing dietary fiber content in sesame (Sesamum indicum L.) 高分辨率全基因组关联研究揭示两个影响芝麻膳食纤维含量的基因

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-13 DOI: 10.1002/csc2.21438

Guiting Li, Hengchun Cao, Weifei Yang, Ming Ju, Qin Ma, Cuiying Wang, Zhanyou Zhang, Qiuzhen Tian, Jiayuan Chen, Qingli Yuan, Hua Du, Hongmei Miao, Haiyang Zhang

{"title":"High-resolution genome-wide association study reveals two genes influencing dietary fiber content in sesame (Sesamum indicum L.)","authors":"Guiting Li, Hengchun Cao, Weifei Yang, Ming Ju, Qin Ma, Cuiying Wang, Zhanyou Zhang, Qiuzhen Tian, Jiayuan Chen, Qingli Yuan, Hua Du, Hongmei Miao, Haiyang Zhang","doi":"10.1002/csc2.21438","DOIUrl":"https://doi.org/10.1002/csc2.21438","url":null,"abstract":"Dietary fiber is widely recognized for its beneficial effects on human health, and sesame (Sesamum indicum L.) seeds are an important source of dietary fiber. However, efforts to improve sesame for higher fiber have been constrained by limited knowledge of the genetic factors underlying this trait. In this study, we identified a strong correlation between fiber and sugar content, both of which are significantly influenced by genetic factors. A large-scale analysis of 222 sesame germplasm resources revealed genome-wide single nucleotide polymorphism (n = 1,535,018), insertions and deletions (InDels; n = 325,371), and structure variations (SVs; n = 29,028). Linkage disequilibrium and genome-wide association studies identified an InDel quantitative trait locus targeting the SiLAC3 gene, which caused a gene frameshift mutation significantly associated with both sugar and dietary fiber traits. Furthermore, a 1201-bp deletion SV in the coding and 3′ untranslated regions of the SiXTH30 gene was associated with high fiber content. Notably, two accessions (CX150 and CX546) with pyramiding favorable alleles of these two mutated genes exhibited a significant increase in fiber content compared to accessions with only one favorable allele. We also found two candidate genes highly expressed during the early and middle stages of seed development, both of which have been previously reported to play important roles in cell wall fiber content. In conclusion, our findings highlight two key fiber-associated candidate genes, which could serve as valuable resources for molecular breeding aimed at increasing fiber content in sesame seeds.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study for traits related to cold tolerance and recovery during seedling stage in rice 水稻苗期抗寒与恢复相关性状的全基因组关联研究

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-13 DOI: 10.1002/csc2.70003

Khushboo Rastogi, Sumeet P. Mankar, Endang M. Septiningsih

{"title":"Genome-wide association study for traits related to cold tolerance and recovery during seedling stage in rice","authors":"Khushboo Rastogi, Sumeet P. Mankar, Endang M. Septiningsih","doi":"10.1002/csc2.70003","DOIUrl":"https://doi.org/10.1002/csc2.70003","url":null,"abstract":"Rice (Oryza sativa L.), due to its tropical and subtropical origin, is adversely affected by low temperatures below 15°C, restricting growth and yield. During the seedling and vegetative stage, cold stress causes curled leaves, fewer tillers, retarded growth, chlorosis, and necrosis. Due to the significance of this trait, exploring the wealth of genetic resources from a wider pool of germplasm to identify new sources of tolerance will be a crucial step. Toward this goal, we performed a genome-wide association study (GWAS) on a novel diversity panel of 238 rice accessions using the 7K SNP Cornell-IR LD Rice (C7AIR) single nucleotide polymorphism (SNP) array. The experiment was conducted in controlled growth conditions, and rice accessions were evaluated for seven traits and four indices, which were recorded at three different time points, that is, before cold treatment, after cold treatment, and post-recovery phase. A total of 77 significant GWAS-quantitative trait loci (QTLs) were identified for 21 cold tolerance and related traits of interest. Out of these, 34 were detected after cold stress, and 43 were detected during the post-recovery phase. A total of 42 QTL regions were significant at false discovery rate (FDR) < 0.001 and −log10(p-values) > 5. In addition, 31 novel GWAS-QTLs, three colocalized GWAS-QTL hotspots, and eight colocalized GWAS-QTLs for two traits sharing the same genomic location were also identified. The results of this study may help in further elucidating the molecular mechanisms underlying cold tolerance as well as aid in developing climate-smart rice varieties for chilling stress conditions.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0