Current Medical Science最新文献_第9页

Rates, Risk Factors, and Outcomes of Nonvariceal Upper Gastrointestinal Bleeding in Patients Hospitalized for COVID-19 in the United States. 美国 COVID-19 住院患者非静脉性上消化道出血的发生率、风险因素和结果。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-12-14 DOI: 10.1007/s11596-024-2838-6

Mohammad Aldiabat, Wesam Aleyadeh, Taimur Muzammil, Kemi Adewuyi, Majd Alahmad, Ahmad Jabri, Laith Alhuneafat, Yassine Kilani, Saqr Alsakarneh, Mohammad Bilal

{"title":"Rates, Risk Factors, and Outcomes of Nonvariceal Upper Gastrointestinal Bleeding in Patients Hospitalized for COVID-19 in the United States.","authors":"Mohammad Aldiabat, Wesam Aleyadeh, Taimur Muzammil, Kemi Adewuyi, Majd Alahmad, Ahmad Jabri, Laith Alhuneafat, Yassine Kilani, Saqr Alsakarneh, Mohammad Bilal","doi":"10.1007/s11596-024-2838-6","DOIUrl":"10.1007/s11596-024-2838-6","url":null,"abstract":"Objective: This study aimed to investigate the incidence and predictors of non-variceal upper gastrointestinal bleeding (NVUGIB) in hospitalized patients with coronavirus disease 2019 (COVID-19), as well as the inpatient outcomes associated with this complication.Methods: This was an analysis of the National Inpatient Sample Database from January to December 2020. Adult COVID-19 patients were categorized into two groups based on NVUGIB development during hospitalization. Multivariate logistic analysis was performed to identify predictors and outcomes associated with NVUGIB in hospitalized COVID-19 patients in the US, after adjusting for age, sex, race, and Charlson Comorbidity Index (CCI) score, using Stata/BE 17.0.Results: Among 1 050 045 hospitalized patients, 1.87% developed NVUGIB. Asian Americans had the highest risk, followed by Native Americans, Hispanics, and African Americans, with odds ratios (ORs) of 1.70, 1.59, 1.40, and 1.14, respectively. Patients with higher CCI scores were also at greater risk (with ORs of 1.47, 2.09, and 3.45 for CCI scores of 1, 2, and 3, respectively). COVID-19 patients with NVUGIB had a higher risk of inpatient mortality (OR=3.84), acute kidney injury (OR=3.12), hypovolemic shock (OR=13.7), blood transfusion (OR=7.02), and in-hospital cardiac arrest (OR=4.02).Conclusion: NVUGIB occurred in 1.87% of hospitalized COVID-19 patients and was associated with a threefold increase in mortality. Further research is necessary to identify strategies for reducing its incidence in COVID-19 patients with multiple risk factors.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1202-1209"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraperitoneal and Extraperitoneal Pringle Hepatic Hilar Occlusion in Laparoscopic Liver Resection: A Prospective Randomized Controlled Study. 腹腔镜肝切除术中的腹膜内和腹膜外普林格尔肝门闭塞术：前瞻性随机对照研究。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-11-20 DOI: 10.1007/s11596-024-2942-7

Liang He, Wei-Xiang Li, Da-Chen Zhou, Yong Wang, Xiao-Ping Geng, Min Yang, Jiong Gu, Hui Hou

{"title":"Intraperitoneal and Extraperitoneal Pringle Hepatic Hilar Occlusion in Laparoscopic Liver Resection: A Prospective Randomized Controlled Study.","authors":"Liang He, Wei-Xiang Li, Da-Chen Zhou, Yong Wang, Xiao-Ping Geng, Min Yang, Jiong Gu, Hui Hou","doi":"10.1007/s11596-024-2942-7","DOIUrl":"10.1007/s11596-024-2942-7","url":null,"abstract":"Objective: This prospective randomized controlled study was conducted to evaluate the safety and efficacy of the Pringle hepatic hilar occlusion with a bulldog clamp in laparoscopic liver resection.Methods: From March 1, 2020 to July 31, 2021, 80 patients were enrolled, including 40 undergoing intraperitoneal Pringle maneuver (IPM) and 40 extraperitoneal Pringle maneuver (EPM). The observation indices included basic preoperative clinical characteristics and intraoperative and postoperative liver function indices.Results: There were no significant differences in the basic characteristics or types of hepatectomy, intraoperative blood loss, intraoperative blood transfusion, or hepatectomy time between the IPM and EPM groups. However, the blocking and operation time in the IPM group was shorter than that in the EPM group. There were no significant differences in alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels on the first day after surgery or in total bilirubin (TBIL) or albumin (ALB) levels on the first, third, or fifth days after surgery. However, C-reactive protein (CRP) levels on the first and third days, ALT and AST levels on the third and fifth days were lower, and hospital stay after surgery was shorter in the IPM group than in the EPM group.Conclusion: IPM using bulldog clamps is simple, safe, and effective. The inflammatory reaction is less severe, the degree of liver function injury is lower, and recovery is faster.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1210-1216"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of ECT2 to Tubulointerstitial Fibrosis in the Progression of Chronic Kidney Disease. ECT2 在慢性肾病进展过程中对肾小管间质纤维化的贡献

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-26 DOI: 10.1007/s11596-024-2948-1

Kai-Xin Song, Hua Su

{"title":"Contribution of ECT2 to Tubulointerstitial Fibrosis in the Progression of Chronic Kidney Disease.","authors":"Kai-Xin Song, Hua Su","doi":"10.1007/s11596-024-2948-1","DOIUrl":"10.1007/s11596-024-2948-1","url":null,"abstract":"Objective: Chronic kidney disease (CKD) is a complex disorder resulting from a combination of various environmental and genetic factors. Considerable efforts have been dedicated to elucidating its etiological mechanisms. Nevertheless, the pathogenic mechanism of CKD remains poorly understood, which hinders the development of effective therapeutic strategies. In this study, we aimed to identify novel mediators that could contribute to the development of CKD.Methods: The ClinVar, STRING, MEME Suite, TRRUST, bedtools, GEO, and R Studio databases and software were used to analyze their common features and investigate potential CKD disease genes. Transcriptomic analysis, immunohistochemistry, qRT-PCR, and Western blotting were utilized to further validate the role of ECT2 in kidney fibrosis.Results: In total, 26 CKD disease genes were obtained from the ClinVar database, and the STRING, MEME Suite, TRRUST, bedtools, and GEO databases and software were used to analyze their common properties and explore potential CKD disease genes. Epithelial cell transforming sequence 2 (ECT2), cyclin B 1, caspase 7 and collagen alpha-1 (IV) were identified as potential candidates for CKD progression. Weighted correlation network analysis (WGCNA) subsequently revealed the relationships between potential genes and CKD. The results of the transcriptomic analysis further confirmed that ECT2 expression was greater in the kidney tissue of CKD patients than in that of healthy controls. Next, immunohistochemistry and Western blotting demonstrated that ECT2 was predominantly expressed in the renal tubules of a unilateral ureteral obstruction (UUO) mouse model. Consistently, in vitro experiments revealed that ECT2 was upregulated in TGF-β1-treated HK-2 cells. Moreover, ECT2 overexpression or knockdown in HK-2 cells altered the intensity of fibrosis markers.Conclusion: ECT2 significantly affects the development and progression of CKD, particularly in association with tubulointerstitial fibrosis.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1249-1258"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance Assessment of GPT 4.0 on the Japanese Medical Licensing Examination. 日本医师资格考试 GPT 4.0 的成绩评估。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-26 DOI: 10.1007/s11596-024-2932-9

Hong-Lin Wang, Hong Zhou, Jia-Yao Zhang, Yi Xie, Jia-Ming Yang, Ming-di Xue, Zi-Neng Yan, Wen Li, Xi-Bao Zhang, Yong Wu, Xiao-Ling Chen, Peng-Ran Liu, Lin Lu, Zhe-Wei Ye

{"title":"Performance Assessment of GPT 4.0 on the Japanese Medical Licensing Examination.","authors":"Hong-Lin Wang, Hong Zhou, Jia-Yao Zhang, Yi Xie, Jia-Ming Yang, Ming-di Xue, Zi-Neng Yan, Wen Li, Xi-Bao Zhang, Yong Wu, Xiao-Ling Chen, Peng-Ran Liu, Lin Lu, Zhe-Wei Ye","doi":"10.1007/s11596-024-2932-9","DOIUrl":"10.1007/s11596-024-2932-9","url":null,"abstract":"Objective: To evaluate the accuracy and parsing ability of GPT 4.0 for Japanese medical practitioner qualification examinations in a multidimensional way to investigate its response accuracy and comprehensiveness to medical knowledge.Methods: We evaluated the performance of the GPT 4.0 on Japanese Medical Licensing Examination (JMLE) questions (2021-2023). Questions are categorized by difficulty and type, with distinctions between general and clinical parts, as well as between single-choice (MCQ1) and multiple-choice (MCQ2) questions. Difficulty levels were determined on the basis of correct rates provided by the JMLE Preparatory School. The accuracy and quality of the GPT 4.0 responses were analyzed via an improved Global Qualily Scale (GQS) scores, considering both the chosen options and the accompanying analysis. Descriptive statistics and Pearson Chi-square tests were used to examine performance across exam years, question difficulty, type, and choice. GPT 4.0 ability was evaluated via the GQS, with comparisons made via the Mann-Whitney U or Kruskal-Wallis test.Results: The correct response rate and parsing ability of the GPT4.0 to the JMLE questions reached the qualification level (80.4%). In terms of the accuracy of the GPT4.0 response to the JMLE, we found significant differences in accuracy across both difficulty levels and option types. According to the GQS scores for the GPT 4.0 responses to all the JMLE questions, the performance of the questionnaire varied according to year and choice type.Conclusion: GTP4.0 performs well in providing basic support in medical education and medical research, but it also needs to input a large amount of medical-related data to train its model and improve the accuracy of its medical knowledge output. Further integration of ChatGPT with the medical field could open new opportunities for medicine.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1148-1154"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Intelligence in Medical Metaverse: Applications, Challenges, and Future Prospects. 人工智能在医疗领域的应用：应用、挑战和未来展望。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-12-14 DOI: 10.1007/s11596-024-2960-5

Jia-Ming Yang, Bao-Jun Chen, Rui-Yuan Li, Bi-Qiang Huang, Mo-Han Zhao, Peng-Ran Liu, Jia-Yao Zhang, Zhe-Wei Ye

{"title":"Artificial Intelligence in Medical Metaverse: Applications, Challenges, and Future Prospects.","authors":"Jia-Ming Yang, Bao-Jun Chen, Rui-Yuan Li, Bi-Qiang Huang, Mo-Han Zhao, Peng-Ran Liu, Jia-Yao Zhang, Zhe-Wei Ye","doi":"10.1007/s11596-024-2960-5","DOIUrl":"10.1007/s11596-024-2960-5","url":null,"abstract":"The medical metaverse is a combination of medicine, computer science, information technology and other cutting-edge technologies. It redefines the method of information interaction about doctor-patient communication, medical education and research through the integration of medical data, knowledge and services in a virtual environment. Artificial intelligence (AI) is a discipline that uses computer technology to study and develop human intelligence. AI has infiltrated every aspect of medical metaverse and is deeply integrated with the technologies that build medical metaverse, such as large language models (LLMs), digital twins, blockchain and extended reality (including VR/AR/XR). AI has become an integral part of the medical metaverse building process. Moreover, AI also provides richer medical metaverse functions, including diagnosis, education, and consulting. This paper aims to introduce how AI supports the development of medical metaverse, including its specific application scenarios, shortcomings and future development. Our goal is to contribute to the advancement of more sophisticated and intelligent medical methods.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1113-1122"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FKBP5 Regulates the Osteogenesis of Human Adipose-derived Mesenchymal Stem Cells. FKBP5 调控人脂肪间充质干细胞的成骨过程

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-11-26 DOI: 10.1007/s11596-024-2941-8

Xiao-Yu Tian, Biao Zhu, Wen-Can Fang, Xiang-Bin Zhou, Ning Wu, Hong Li, Ning Wen, Jin Li

{"title":"FKBP5 Regulates the Osteogenesis of Human Adipose-derived Mesenchymal Stem Cells.","authors":"Xiao-Yu Tian, Biao Zhu, Wen-Can Fang, Xiang-Bin Zhou, Ning Wu, Hong Li, Ning Wen, Jin Li","doi":"10.1007/s11596-024-2941-8","DOIUrl":"10.1007/s11596-024-2941-8","url":null,"abstract":"Objective: Human adipose-derived stem cells (ASCs) have shown considerable potential for tissue regeneration. FK506 binding protein (FKBP) 5 is a cochaperone of several proteins. The purpose of this work was to explore the function of FKBP5 in ASC osteogenesis.Methods: Lentivirus infection was used to overexpress or knock down FKBP5 in ASCs. To inhibit FKBP5, SAFit2, a specific inhibitor of FKBP5, was used. Next, the osteogenic capacity of ASCs was evaluated via alkaline phosphatase (ALP) staining, and extracellular calcium precipitation was detected via Alizarin red S staining. The binding proteins of FKBP5 were assessed via proteomics and validated via coimmunoprecipitation experiments.Results: Following osteogenic induction, FKBP5 expression increased at both the mRNA and protein levels. Interestingly, FKBP5 upregulation by lentivirus infection increased the ability of ASCs to differentiate into osteoblasts, as revealed by ALP staining, while ALP activity also increased. Moreover, increased extracellular calcium precipitation confirmed that FKBP5 overexpression promoted ASC osteogenesis into osteocytes. On the other hand, FKBP5 knockdown or functional suppression with SAFit2 decreased this process. Furthermore, the proteomics and coimmunoprecipitation data demonstrated that FKBP5 bound to a variety of proteins in ASCs. These proteins serve as the molecular chaperone base upon which the osteogenesis-regulating activity of FKBP5 rests.Conclusion: Our study revealed that FKBP5 enhances the osteogenesis of ASCs, providing a feasible method for clinical bone tissue engineering applications.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1270-1279"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain-computer Interaction in the Smart Era. 智能时代的脑机交互。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-09-30 DOI: 10.1007/s11596-024-2927-6

Zi-Neng Yan, Peng-Ran Liu, Hong Zhou, Jia-Yao Zhang, Song-Xiang Liu, Yi Xie, Hong-Lin Wang, Jin-Bo Yu, Yu Zhou, Chang-Mao Ni, Li Huang, Zhe-Wei Ye

{"title":"Brain-computer Interaction in the Smart Era.","authors":"Zi-Neng Yan, Peng-Ran Liu, Hong Zhou, Jia-Yao Zhang, Song-Xiang Liu, Yi Xie, Hong-Lin Wang, Jin-Bo Yu, Yu Zhou, Chang-Mao Ni, Li Huang, Zhe-Wei Ye","doi":"10.1007/s11596-024-2927-6","DOIUrl":"10.1007/s11596-024-2927-6","url":null,"abstract":"The brain-computer interface (BCI) system serves as a critical link between external output devices and the human brain. A monitored object's mental state, sensory cognition, and even higher cognition are reflected in its electroencephalography (EEG) signal. Nevertheless, unprocessed EEG signals are frequently contaminated with a variety of artifacts, rendering the analysis and elimination of impurities from the collected EEG data exceedingly challenging, not to mention the manual adjustment thereof. Over the last few decades, the rapid advancement of artificial intelligence (AI) technology has contributed to the development of BCI technology. Algorithms derived from AI and machine learning have significantly enhanced the ability to analyze and process EEG electrical signals, thereby expanding the range of potential interactions between the human brain and computers. As a result, the present BCI technology with the help of AI can assist physicians in gaining a more comprehensive understanding of their patients' physical and psychological status, thereby contributing to improvements in their health and quality of life.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1123-1131"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Combinatorial Therapeutic Aspects: The Future Prospect for Glioblastoma Treatment. 目前的组合治疗方面：胶质母细胞瘤治疗的未来展望。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-12-19 DOI: 10.1007/s11596-024-2950-7

Megha Gautam, Reema Gabrani

引用次数: 0

Advancing the Battle against Cystic Fibrosis: Stem Cell and Gene Therapy Insights. 推进囊性纤维化的防治工作：干细胞和基因疗法的启示。

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-12-16 DOI: 10.1007/s11596-024-2936-5

Disha D Shah, Mehul R Chorawala, Aanshi J Pandya, Nirjari Kothari, Bhupendra G Prajapati, Priyajeet S Parekh

{"title":"Advancing the Battle against Cystic Fibrosis: Stem Cell and Gene Therapy Insights.","authors":"Disha D Shah, Mehul R Chorawala, Aanshi J Pandya, Nirjari Kothari, Bhupendra G Prajapati, Priyajeet S Parekh","doi":"10.1007/s11596-024-2936-5","DOIUrl":"10.1007/s11596-024-2936-5","url":null,"abstract":"Cystic fibrosis (CF) is a hereditary disorder characterized by mutations in the CFTR gene, leading to impaired chloride ion transport and subsequent thickening of mucus in various organs, particularly the lungs. Despite significant progress in CF management, current treatments focus mainly on symptom relief and do not address the underlying genetic defects. Stem cell and gene therapies present promising avenues for tackling CF at its root cause. Stem cells, including embryonic, induced pluripotent, mesenchymal, hematopoietic, and lung progenitor cells, offer regenerative potential by differentiating into specialized cells and modulating immune responses. Similarly, gene therapy aims to correct CFTR gene mutations by delivering functional copies of the gene into affected cells. Various approaches, such as viral and nonviral vectors, gene editing with CRISPR-Cas9, small interfering RNA (siRNA) therapy, and mRNA therapy, are being explored to achieve gene correction. Despite their potential, challenges such as safety concerns, ethical considerations, delivery system optimization, and long-term efficacy remain. This review provides a comprehensive overview of the current understanding of CF pathophysiology, the rationale for exploring stem cell and gene therapies, the types of therapies available, their mechanisms of action, and the challenges and future directions in the field. By addressing these challenges, stem cell and gene therapies hold promise for transforming CF management and improving the quality of life of affected individuals.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1155-1174"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA H19 Activates the RAS-MAPK Signaling Pathway via miR-140-5p/SOS1 Axis in Malignant Liver Tumors. LncRNA H19 通过 miR-140-5p/SOS1 轴激活恶性肝肿瘤中的 RAS-MAPK 信号通路

IF 2 4区医学

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-11-20 DOI: 10.1007/s11596-024-2949-0

Ya-Qun Yu, Shu-Qun Li, Jun Weng, Bo Li, Li-Ling Qin, Jun Lv

{"title":"LncRNA H19 Activates the RAS-MAPK Signaling Pathway via miR-140-5p/SOS1 Axis in Malignant Liver Tumors.","authors":"Ya-Qun Yu, Shu-Qun Li, Jun Weng, Bo Li, Li-Ling Qin, Jun Lv","doi":"10.1007/s11596-024-2949-0","DOIUrl":"10.1007/s11596-024-2949-0","url":null,"abstract":"Objective: To study the influences of LncRNA H19 (H19) on malignant liver tumor cells and elucidate the underlying molecular mechanisms.Methods: H19 expression in liver tumor tissues, matched normal liver tissues, human liver malignant tumor cell lines and the human hepatocyte line LO2 was assessed via quantitative RT-PCR. Cell viability analysis and Matrigel invasion analysis were performed to evaluate the effects of H19 on cell proliferation and invasion. Luciferase reporter analysis was carried out to assess the interaction between miR-140-5p and SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1). The influence of H19 on the Ras-MAPK signalling pathway was evaluated by detecting key protein levels via active Ras pull-down analysis and Western blot analysis.Results: H19 expression was lower in liver cancer samples than in matched normal liver tissue samples. H19 overexpression enhanced the proliferation and invasion of HepG2 and SMMC-7721 cells. H19 overexpression increased the level of activated Ras. The expression levels of phosphorylated Raf, phosphorylated ERK and phosphorylated MEK were increased by H19 overexpression. H19 knockdown had the opposite effect. Treatment with a MAPK inhibitor significantly reversed the influence of H19 overexpression on liver malignant tumor cell growth and invasion. The MAPK activator reversed the opposing effects of H19 silencing. H19 overexpression increased the protein level of SOS1, and miR-140-5p directly targeted SOS1.Conclusion: H19 can activate the Ras-MAPK signalling pathway via the miR-140-5p/SOS1 axis in malignant liver tumour cells.","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1232-1240"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0