Crop Journal最新文献

{"title":"R3-MYB proteins OsTCL1 and OsTCL2 modulate seed germination via dual pathways in rice","authors":"Yong Yi ,&nbsp;Chan Lin ,&nbsp;Xueyan Peng ,&nbsp;Meishan Zhang ,&nbsp;Jiaming Wu ,&nbsp;Chunmei Meng ,&nbsp;Shengchao Ge ,&nbsp;Yunfeng Liu ,&nbsp;Yuan Su","doi":"10.1016/j.cj.2023.10.004","DOIUrl":"10.1016/j.cj.2023.10.004","url":null,"abstract":"<div><p>Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination, potentially leading to significantly impaired growth and production in rice. Gaining a comprehensive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount importance. In the present study, we identified two R3-MYB genes in rice, namely <em>OsTCL1</em> and <em>OsTCL2</em>, and characterized their roles in regulating seed germination under salt and drought stress. Plants with <em>tcl1</em> and <em>tcl2</em> mutant alleles exhibited delayed seed germination, particularly under stress conditions. The <em>tcl1 tcl2</em> double mutant showed an even more pronounced reduction in germination during initial stages of germination, thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses. Furthermore, we demonstrated that the transcript levels of several phospholipase D (PLD) genes were downregulated in the <em>tcl1 tcl2</em> mutant, resulting in a decreased level of the phosphatidic acid (PA) product. Application of 1-butanol, a competitive substrate inhibitor of PLD-dependent production of PA, attenuated the stress response of the <em>tcl1 tcl2</em> mutant. This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway. Moreover, there were alterations in the expression of genes involved in abscisic acid (ABA) biosynthesis, metabolism and signaling transduction in the double mutant. These changes affected the endogenous ABA level and ABA response, thereby influencing seed germination. Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate (Na₂WO₄) nearly eliminated the differences in stress response between wild type and the <em>tcl1 tcl2</em> mutant. This indicates that OsTCL1 and OsTCL2 synergistically coordinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001411/pdfft?md5=4d062482c21f7efea550c48ea36d6a4f&pid=1-s2.0-S2214514123001411-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly and phylogenomic analysis of cotton mitochondrial genomes provide insights into the history of cotton evolution","authors":"Yanlei Feng ,&nbsp;Yukang Wang ,&nbsp;Hejun Lu ,&nbsp;Jun Li ,&nbsp;Delara Akhter ,&nbsp;Fang Liu ,&nbsp;Ting Zhao ,&nbsp;Xingxing Shen ,&nbsp;Xiaobo Li ,&nbsp;James Whelan ,&nbsp;Tianzhen Zhang ,&nbsp;Jianping Hu ,&nbsp;Ronghui Pan","doi":"10.1016/j.cj.2023.05.004","DOIUrl":"10.1016/j.cj.2023.05.004","url":null,"abstract":"<div><p>Cotton is a major crop that provides the most important renewable textile fibers in the world. Studies of the taxonomy and evolution of cotton species have received wide attentions, not only due to cotton’s economic value but also due to the fact that <em>Gossypium</em> is an ideal model system to study the origin, evolution, and cultivation of polyploid species. Previous studies suggested the involvement of mitochondrial genome editing sites and copy number as well as mitochondrial functions in cotton fiber elongation. Whereas, with only a few mitogenomes assembled in the cotton genus <em>Gossypium</em>, our knowledge about their roles in cotton evolution and speciation is still scarce. To close this gap, here we assembled 20 mitogenomes from 15 cotton species spanning all the cotton clades (A–G, K, and AD genomes) and 5 cotton relatives using short and long sequencing reads. Systematic analyses uncovered a high level of mitochondrial gene sequence conservation, abundant sequence repeats and many insertions of foreign sequences, as well as extensive structural variations in cotton mitogenomes. The sequence repeats and foreign sequences caused significant mitogenome size inflation in <em>Gossypium</em> and its close relative <em>Kokia</em> in general, while there is no significant difference between the lint and fuzz cotton mitogenomes in terms of gene content, RNA editing, and gene expression level. Interestingly, we further revealed the specific presence and expression of two novel mitochondrial open reading frames (ORFs) in lint-fiber cotton species. Finally, these structural features and novel ORFs help us gain valuable insights into the history of cotton evolution and polyploidization and the origin of species producing long lint fibers from a mitogenomic perspective.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123000752/pdfft?md5=3c2a66969442433e8a54dbea4fb9d85e&pid=1-s2.0-S2214514123000752-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133496766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miR5810/OsMRLP6 regulatory module affects rice seedling photosynthesis","authors":"Weiwei Gao ,&nbsp;Mingkang Li ,&nbsp;Huaping Cheng ,&nbsp;Kuaifei Xia ,&nbsp;Mingyong Zhang","doi":"10.1016/j.cj.2023.08.004","DOIUrl":"10.1016/j.cj.2023.08.004","url":null,"abstract":"<div><p>Photosynthesis affects crop growth and yield. The roles of microRNAs (miRNAs) in photosynthesis are little known. In the present study, the role of the OsNF-YB7<em>–OsMIR5810–OsMRLP6</em> regulatory module in photosynthesis was investigated. The malectin-like protein gene <em>OsMRLP6</em> was identified as a target gene of osa-miR5810 (miR5810). Overexpression in rice of miR5810 or down-expression of <em>OsMRLP6</em> resulted in reduced expression of genes involved in chloroplast development and photosynthesis and decreased net photosynthetic rate, finally leading to lower shoot biomass and grain yield. Down-expression of miR5810 and overexpression of <em>OsMRLP6</em> showed the opposite effect. Overexpression of transcription factor <em>OsNF-YB7</em> elevated expression of <em>OsMIR5810</em> in rice seedlings by binding to its promoter. The OsNF-YB7<em>–OsMIR5810–OsMRLP6</em> regulatory module affects photosynthesis to mediate growth and grain yield.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001149/pdfft?md5=8b8359e5a947d0689a88bde2a9209388&pid=1-s2.0-S2214514123001149-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135389975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of soybean stem growth habit: A ten-year progress report","authors":"Shang-Shang Xiong ,&nbsp;Dan-Dan Guo ,&nbsp;Zhao Wan ,&nbsp;Le Quan ,&nbsp;Wen-Tian Lu ,&nbsp;Yongguo Xue ,&nbsp;Baohui Liu ,&nbsp;Hong Zhai","doi":"10.1016/j.cj.2023.08.002","DOIUrl":"10.1016/j.cj.2023.08.002","url":null,"abstract":"<div><p>Stem growth habit dictates plant architecture and influences flowering and podding (seed setting), making it an essential morphological and breeding agronomic trait of soybean (<em>Glycine max</em>). Stem growth habit in soybean is affected by photoperiod and environment and is determined by genetic variation at major genes. Classical genetic analysis identified two critical loci, designated <em>Determinacy 1</em> (<em>Dt1</em>) and <em>Determinacy 2</em> (<em>Dt2</em>). <em>Dt1</em> is an ortholog of <em>Arabidopsis thaliana</em> TERMINAL FLOWER1 (TFL1) and specifies an indeterminate stem growth habit, whereas <em>Dt2</em> specifies a semi-determinate growth habit. MADS-box proteins, including Dt2, SUPPRESSOR OF OVEREXPRESSION OF CO1 (GmSOC1) and MADS-box genes downregulated by E1 (<em>GmMDE</em>), repress <em>Dt1</em> expression. Photoreceptors encoded by the <em>E3</em> and <em>E4</em> loci regulate the expression of soybean <em>FLOWERING LOCUS T</em> (<em>GmFT</em>) orthologs via circadian clock genes and E1, and GmFTs compete with Dt1 to regulate stem growth habit. Study of the molecular mechanism underlying the regulation of stem growth habit in soybean has focused on the repression of <em>Dt1</em> expression. Here we provide an overview of progress made in elucidating the genetic and molecular bases of stem growth habit in soybean, with emphasis on the molecular components responsible for integrating photoperiodic flowering and stem growth habit.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001125/pdfft?md5=ad0bdc36fcc037fe0ef2eff98376e7ba&pid=1-s2.0-S2214514123001125-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135248171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dynamic regulation of nitrogen on floret primordia development in wheat","authors":"Zhen Zhang, Yujing Li, Yuxun Wu, Xiaoyu Zheng, Xiaolei Guo, Wan Sun, Zhencai Sun, Zhimin Wang, Yinghua Zhang","doi":"10.1016/j.cj.2023.10.009","DOIUrl":"https://doi.org/10.1016/j.cj.2023.10.009","url":null,"abstract":"Nitrogen (N) fertilization is critical for spike and floret development, which affects the number of fertile florets per spike (NFFs). However, the physiological regulation of the floret development process by N fertilization is largely unknown. A high temporal-resolution investigation of floret primordia number and morphology, dry matter, and N availability was conducted under three N fertilization levels: 0 (N0), 120 (N1) and 240 (N2) kg ha−1. Interestingly, fertile florets at anthesis stage were determined by those floret primordia with meiotic ability at booting stage: meiotic ability was a threshold that predicted whether a floret primordium became fertile or abortive florets. Because the developmental rate of the 4th floret primordium in the central spikelet was accelerated and then they acquired meiotic ability, the NFFs increased gradually as N application increased, but the increase range decreased under N2. There were no differences in spike N concentration among treatments, but leaf N concentration was increased in the N1 and N2 treatments. Correspondingly, dry matter accumulation and N content of the leaf and spike in the N1 and N2 treatments was increased as compared to N0. Clearly, optimal N fertilization increased leaf N availability and transport of assimilates to spikes, and allowed more floret primordia to acquire meiotic ability and become fertile florets, finally increasing NFFs. There was no difference in leaf N concentration between N1 and N2 treatment, whereas soil N concentration at 0-60 cm soil layers was higher in N2 than in N1 treatment, implying that there was still some N fertilization that remained unused. Therefore, improving the leaf’s ability to further use N fertilizer is vital for greater NFFs.","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135566238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remote sensing of quality traits in cereal and arable production systems: A review","authors":"Zhenhai Li, Chengzhi Fan, Yu Zhao, Xiuliang Jin, Raffaele Casa, Wenjiang Huang, Xiaoyu Song, Gerald Blasch, Guijun Yang, James Taylor, Zhenhong Li","doi":"10.1016/j.cj.2023.10.005","DOIUrl":"https://doi.org/10.1016/j.cj.2023.10.005","url":null,"abstract":"Cereal is an essential source of calories and protein for the global population. Accurately predicting cereal quality before harvest is highly desirable in order to optimise management for farmers, grading harvest and categorised storage for enterprises, future trading prices, and policy planning. The use of remote sensing data with extensive spatial coverage demonstrates some potential in predicting crop quality traits. Many studies have also proposed models and methods for predicting such traits based on multi-platform remote sensing data. In this paper, the key quality traits that are of interest to producers and consumers are introduced. The literature related to grain quality prediction was analyzed in detail, and a review was conducted on remote sensing platforms, commonly used methods, potential gaps, and future trends in crop quality prediction. This review recommends new research directions that go beyond the traditional methods and discusses grain quality retrieval and the associated challenges from the perspective of remote sensing data.","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135713893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A telomere-to-telomere genome assembly of Zhonghuang 13, a widely-grown soybean variety from the original center of Glycine max","authors":"Anqi Zhang, Tangchao Kong, Baiquan Sun, Shizheng Qiu, Jiahe Guo, Shuyong Ruan, Yu Guo, Jirui Guo, Zhishuai Zhang, Yue Liu, Zheng Hu, Tao Jiang, Yadong Liu, Shuqi Cao, Shi Sun, Tingting Wu, Huilong Hong, Bingjun Jiang, Maoxiang Yang, Xiangyu Yao, Yang Hu, Bo Liu, Tianfu Han, Yadong Wang","doi":"10.1016/j.cj.2023.10.003","DOIUrl":"https://doi.org/10.1016/j.cj.2023.10.003","url":null,"abstract":"Soybean (Glycine max) stands as a globally significant agricultural crop, and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary history. Nevertheless, previous soybean genome assemblies have harbored gaps and incompleteness, which have constrained in-depth investigations into soybean. Here, we present Telomere-to-Telomere (T2T) assembly of the Chinese soybean cultivar Zhonghuang 13 (ZH13) genome, termed ZH13-T2T, utilizing PacBio Hifi and ONT ultralong reads. We employed a multi-assembler approach, integrating Hifiasm, NextDenovo, and Canu, to minimize biases and enhance assembly accuracy. The assembly spans 1,015,024,879 bp, effectively resolving all 393 gaps that previously plagued the reference genome. Our annotation efforts identified 50,564 high-confidence protein-coding genes, 707 of which are novel. ZH13-T2T revealed longer chromosomes, 421 not-aligned regions (NARs), 112 structure variations (SVs), and a substantial expansion of repetitive element compared to earlier assemblies. Specifically, we identified 25.67 Mb of tandem repeats, an enrichment of 5S and 48S rDNAs, and characterized their genotypic diversity. In summary, we deliver the first complete Chinese soybean cultivar T2T genome. The comprehensive annotation, along with precise centromere and telomere characterization, as well as insights into structural variations, further enhance our understanding of soybean genetics and evolution.","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135410126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice–wheat comparative genomics: Gains and gaps","authors":"Akila Wijerathna-Yapa, Ruchi Bishnoi, Buddhini Ranawaka, Manu Maya Magar, Hafeez Ur Rehman, Swati G. Bharad, Michal T. Lorenc, Vinita Ramtekey, Sasha Gohar, Charu Lata, Md. Harun-Or-Rashid, Maryam Razzaq, Muhammad Sajjad, Bhoja R. Basnet","doi":"10.1016/j.cj.2023.10.008","DOIUrl":"https://doi.org/10.1016/j.cj.2023.10.008","url":null,"abstract":"Rice and wheat provide nearly 40% of human calorie and protein requirements. They share a common ancestor and belong to the Poaceae (grass) family. Characterizing their genetic homology is crucial for developing new cultivars with enhanced traits. Several wheat genes and gene families have been characterized based on their rice orthologs. Rice–wheat orthology can identify genetic regions that regulate similar traits in both crops. Rice–wheat comparative genomics can identify candidate wheat genes in a genomic region identified by association or QTL mapping, deduce their putative functions and biochemical pathways, and develop molecular markers for marker-assisted breeding. A knowledge of gene homology facilitates the transfer between crops of genes or genomic regions associated with desirable traits by genetic engineering, gene editing, or wide crossing.","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135670216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SimET: An open-source tool for estimating crop evapotranspiration and soil water balance for plants with multiple growth cycles","authors":"Minguo Liu,&nbsp;Mei Yang,&nbsp;Huimin Yang","doi":"10.1016/j.cj.2023.03.008","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.008","url":null,"abstract":"<div><p>Accurate estimation of crop evapotranspiration (ETc) and soil water balance, which is vital for optimizing water management strategy in crop production, can be performed by simulation. But existing software has many deficiencies, including complex operation, limited scalability, lack of batch processing, and a single ETc model. Here we present simET, an open-source software package written in the R programming language. Many concepts involved in crop ETc simulation are condensed into functions in the package. It includes three widely used crop ETc models built on these functions: the single-crop coefficient, double-crop coefficient, and Shuttleworth–Wallace models, along with tools for preparing model data and comparing estimates. SimET supports ETc simulation in crops with repeated growth cycles such as alfalfa, a perennial forage crop that is cut multiple times annually.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49803754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC","authors":"Rongrong Chen ,&nbsp;Qianhan Wei ,&nbsp;Yan Liu ,&nbsp;Jiankun Li ,&nbsp;Xuemei Du ,&nbsp;Yan Chen ,&nbsp;Jianhua Wang ,&nbsp;Yunjun Liu","doi":"10.1016/j.cj.2023.03.004","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.004","url":null,"abstract":"<div><p>Although several pentatricopeptide repeat (PPR) proteins are involved in post-transcriptional processing of mitochondrial RNA, it is unclear which specific protein is involved in the RNA editing of <em>ccmC</em> in maize (<em>Zea mays</em>). Here we report the identification of the maize <em>empty pericarp 601</em> (<em>emp601</em>) mutant and the map-based cloning of the <em>Emp601</em> gene, which encodes an E2-type PPR protein that is targeted to mitochondria. A single-nucleotide deletion in the <em>emp601</em> mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex III as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. We determine that loss of EMP601 function prevents the C-to-U RNA editing of the mitochondrial transcript <em>ccmC</em> at position 358. EMP601 binds to the <em>ccmC</em> transcript and directly interacts with Multiple organellar RNA editing factor 8 and may be a component of the plant mitochondrial editosome. We conclude that EMP601 functions in RNA editing of mitochondrial <em>ccmC</em> transcripts and influences mitochondrial function and seed development.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49803781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}