Yong Yi , Chan Lin , Xueyan Peng , Meishan Zhang , Jiaming Wu , Chunmei Meng , Shengchao Ge , Yunfeng Liu , Yuan Su
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The <em>tcl1 tcl2</em> double mutant showed an even more pronounced reduction in germination during initial stages of germination, thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses. Furthermore, we demonstrated that the transcript levels of several phospholipase D (PLD) genes were downregulated in the <em>tcl1 tcl2</em> mutant, resulting in a decreased level of the phosphatidic acid (PA) product. Application of 1-butanol, a competitive substrate inhibitor of PLD-dependent production of PA, attenuated the stress response of the <em>tcl1 tcl2</em> mutant. This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway. Moreover, there were alterations in the expression of genes involved in abscisic acid (ABA) biosynthesis, metabolism and signaling transduction in the double mutant. These changes affected the endogenous ABA level and ABA response, thereby influencing seed germination. Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate (Na<sub>2</sub>WO<sub>4</sub>) nearly eliminated the differences in stress response between wild type and the <em>tcl1 tcl2</em> mutant. 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Application of 1-butanol, a competitive substrate inhibitor of PLD-dependent production of PA, attenuated the stress response of the <em>tcl1 tcl2</em> mutant. This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway. Moreover, there were alterations in the expression of genes involved in abscisic acid (ABA) biosynthesis, metabolism and signaling transduction in the double mutant. These changes affected the endogenous ABA level and ABA response, thereby influencing seed germination. Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate (Na<sub>2</sub>WO<sub>4</sub>) nearly eliminated the differences in stress response between wild type and the <em>tcl1 tcl2</em> mutant. 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引用次数: 0
摘要
盐胁迫和干旱胁迫是对种子萌发产生不利影响的常见非生物因素,可能导致水稻生长和产量严重受损。全面了解种子在萌发过程中对非生物胁迫的分子反应至关重要。在本研究中,我们鉴定了水稻中的两个 R3-MYB 基因,即 OsTCL1 和 OsTCL2,并描述了它们在盐胁迫和干旱胁迫下调控种子萌发的作用。具有 tcl1 和 tcl2 突变等位基因的植株表现出种子萌发延迟,尤其是在胁迫条件下。tcl1 tcl2双突变体在萌发初期的萌发率下降更为明显,从而表明 OsTCL1 和 OsTCL2 在非生物胁迫下对种子萌发具有冗余调控功能。此外,我们还证明在 tcl1 tcl2 突变体中,几个磷脂酶 D(PLD)基因的转录水平下调,导致磷脂酸(PA)产物水平降低。应用 1-丁醇(PLD 依赖性 PA 生成的竞争性底物抑制剂)可减轻 tcl1 tcl2 突变体的胁迫反应。这表明 OsTCL1 和 OsTCL2 通过 PLD-PA 信号通路部分调节了种子萌发。此外,双突变体中参与脱落酸(ABA)生物合成、代谢和信号转导的基因表达发生了变化。这些变化影响了内源 ABA 水平和 ABA 反应,从而影响了种子萌发。应用 1-丁醇和 ABA 合成抑制剂钨酸钠(Na2WO4)几乎消除了野生型与 tcl1 tcl2 突变体之间在胁迫反应上的差异。这表明 OsTCL1 和 OsTCL2 通过 PLD-PA 信号转导和 ABA 介导的途径协同协调非生物胁迫下的种子萌发。
R3-MYB proteins OsTCL1 and OsTCL2 modulate seed germination via dual pathways in rice
Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination, potentially leading to significantly impaired growth and production in rice. Gaining a comprehensive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount importance. In the present study, we identified two R3-MYB genes in rice, namely OsTCL1 and OsTCL2, and characterized their roles in regulating seed germination under salt and drought stress. Plants with tcl1 and tcl2 mutant alleles exhibited delayed seed germination, particularly under stress conditions. The tcl1 tcl2 double mutant showed an even more pronounced reduction in germination during initial stages of germination, thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses. Furthermore, we demonstrated that the transcript levels of several phospholipase D (PLD) genes were downregulated in the tcl1 tcl2 mutant, resulting in a decreased level of the phosphatidic acid (PA) product. Application of 1-butanol, a competitive substrate inhibitor of PLD-dependent production of PA, attenuated the stress response of the tcl1 tcl2 mutant. This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway. Moreover, there were alterations in the expression of genes involved in abscisic acid (ABA) biosynthesis, metabolism and signaling transduction in the double mutant. These changes affected the endogenous ABA level and ABA response, thereby influencing seed germination. Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate (Na2WO4) nearly eliminated the differences in stress response between wild type and the tcl1 tcl2 mutant. This indicates that OsTCL1 and OsTCL2 synergistically coordinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.