{"title":"Optimization of Malabar Nut (Justicia adhatoda L.) Leaves’ Phenolic\u0000Compounds by Alginate Emulsion-gelation Approach using Response\u0000Surface Methodology","authors":"Afaf Chebout, M. Chaalal, S. Ydjedd, Louiza Himed","doi":"10.2174/0115734072297243240304113132","DOIUrl":"https://doi.org/10.2174/0115734072297243240304113132","url":null,"abstract":"\u0000\u0000The encapsulation technique is an effective method for coating bioactive\u0000molecules and protecting them against various technological treatment conditions during production processing.\u0000\u0000\u0000\u0000The aim of this study was to optimize the encapsulation conditions of phenolic compounds extracted from Malabar nut (Justicia adhatoda L.) leaves by alginate emulsion-gelation\u0000approach using response surface methodology\u0000\u0000\u0000\u0000The ionotropic gelation method was used to encapsulate the phenolic compounds of\u0000Malabar nut (Justicia adhatoda L.) leaves. The optimization of this phenolic compounds encapsulation was carried out using response surface methodology through Box˗Behnken design. Four\u0000parameters with three levels (-1, 0, +1) were chosen including sodium alginate concentration\u0000(2 – 3 - 4%), calcium chloride concentration (4 - 6 - 8%), plant extract-alginate solution ratio\u0000(0.02 – 0.11 - 0.2 mg/ml), and gelation time (5 – 12.5 - 20 min). of the total phenolic and flavonoid contents encapsulation efficiency was assessed. Likewise, the antioxidant activity was evaluated using ferric-reducing power (FRP) and free radical scavenging activity (DPPH).\u0000\u0000\u0000\u0000The results of response surface methodology analysis using Box˗Behnken design\u0000showed that the optimal encapsulation conditions were 3.11% for alginate concentration, 5.74%\u0000for calcium chloride concentration, 0.1 mg/ml for the plant extract-alginate solution ratio, and\u000010.80 min for gelation time. Under these conditions, the optimum values of total phenolic and\u0000flavonoid encapsulation efficiency were 86.17% and 75.69%, respectively, 126.75 mg AAE/100\u0000g for ferric reducing power and 97.29% for DPPH. The experimental and prediction results have\u0000expressed a high significant level for all responses.\u0000\u0000\u0000\u0000The method revealed the validity of elaborated models through response surface\u0000methodology optimization processes for phenolic antioxidants encapsulation of Malabar nut\u0000(Justicia adhatoda L.) leaves extract.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"7 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140248582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}