Connective Tissue Research最新文献_第6页

Comparison of methods for the isolation and culture of Migratory chondroprogenitors from Human articular cartilage. 人关节软骨迁移软骨祖细胞分离与培养方法的比较。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 DOI: 10.1080/03008207.2023.2202266

Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, J Jeya Lisha, Alfred Job Daniel, Solomon Sathishkumar

{"title":"Comparison of methods for the isolation and culture of Migratory chondroprogenitors from Human articular cartilage.","authors":"Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, J Jeya Lisha, Alfred Job Daniel, Solomon Sathishkumar","doi":"10.1080/03008207.2023.2202266","DOIUrl":"https://doi.org/10.1080/03008207.2023.2202266","url":null,"abstract":"Purpose: Resident articular stem cells isolated using a migratory assay called Migratory Chondroprogenitors (MCPs) have emerged as a promising cellular therapeutic for the treatment of cartilage pathologies. In-vivo studies using MCPs report their superiority over bone-marrow mesenchymal stem cells and chondrocytes for treating chondral defects. However, there is no consensus on their isolation protocol. This study aimed to compare four reported isolation methods of MCPs and identify the optimal and feasible protocol for future translational work.Methods: Human MCPs isolated from osteoarthritic cartilage (n = 3) were divided into four groups: a) MCP1: 8-15 mm cartilage explants, b) MCP2: 8-10 mm explants digested in 0.1% collagenase for 2 hrs. and cultured c) MCP3: 1 mm cartilage explants and d) MCP 4: 25 mm explants with a X tear, 7-day culture, and trypsinization to release migrated cells. The MCPs were subjected to the following analysis: growth kinetics, surface marker expression, mRNA gene expression for markers of chondrogenesis and hypertrophy, and trilineage differentiation.Results: MCPs isolated via the four methods showed similar surface marker profiles, chondrogenic (SOX-9, ACAN, COL2A1) and hypertrophic (COL1, RUNX2) gene expression. The migration time for the MCP3 group was the longest. The MCP1, MCP2, and MCP4 groups produced MCPs with comparable cellular expansion feasibility.Conclusions: MCPs can be preferably isolated by the any of the three above methods based on the investigator's discretion. In the case of small cartilage samples similar to the MCP3 group, the isolation of MCP is plausible, keeping in mind the additional time required.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9748896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1: a potential new target for tendinopathy treatment. HMGB1：肌腱病治疗的潜在新靶点。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 Epub Date: 2023-04-09 DOI: 10.1080/03008207.2023.2199089

Panpan Lu, Yingjuan Li, Guangchun Dai, Yuanwei Zhang, Liu Shi, Ming Zhang, Hao Wang, Yunfeng Rui

引用次数: 0

Ovariectomy with simulated vaginal delivery to establish a rat model for pelvic organ prolapse. 卵巢切除模拟阴道分娩建立大鼠盆腔器官脱垂模型。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 DOI: 10.1080/03008207.2023.2199091

Tao Guo, Zhe Du, Xiu-Qi Wang, Jing-He Lang, Zhi-Jing Sun

{"title":"Ovariectomy with simulated vaginal delivery to establish a rat model for pelvic organ prolapse.","authors":"Tao Guo, Zhe Du, Xiu-Qi Wang, Jing-He Lang, Zhi-Jing Sun","doi":"10.1080/03008207.2023.2199091","DOIUrl":"https://doi.org/10.1080/03008207.2023.2199091","url":null,"abstract":"The widespread prevalence of Pelvic Organ Prolapse (POP) and the paucity of ongoing treatments prompted us to develop a unique rat model combining ovariectomy and simulated vaginal delivery. We hypothesized that the tissue changes caused by low hormone levels and mechanical stretch could complement each other. Thus, the combined model can potentially mimic the collagen metabolism of vaginal wall tissue as well as mechanical stretch properties to complement disease progression in POP. Ovariectomy with sequential simulated vaginal delivery was performed on rats in the modeling group. Sham surgeries were performed as control. At 2, 4, and 12 weeks after modeling, the vaginal tissues of rats were evaluated by Masson's trichrome staining, Picro-Sirius red staining, immunohistochemistry, western blotting, and uniaxial tensile tests. Compared to the control group, the vaginal tissues of the model rats showed an atrophic epithelial layer and loose collagen fibers. The smooth muscle fibers were ruptured, smaller in diameter, and disorganized. The ratio of collagen type I/III significantly increased, but the contents of both Collagen I and III decreased. The expression of metalloproteinases 2 and 9 in the tissues increased, and the expression of tissue inhibitors of metalloproteinases 1 and 2 decreased. The tangent modulus of the tissues was significantly increased in the model rats. We verified a novel method to establish a pelvic organ prolapse model in rats. This approach combined the advantages of low hormone levels and mechanical stretch effects.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10125886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tribute to doctor Arthur Veis. 向亚瑟-维斯医生致敬

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 Epub Date: 2023-06-11 DOI: 10.1080/03008207.2023.2213502

Elizabeth Lux

引用次数: 0

β-Arrestin 2 knockout prevents bone loss in response to continuous parathyroid hormone stimulation in male and female mice. 在雄性和雌性小鼠中，β-抑制蛋白2敲除可防止连续甲状旁腺激素刺激导致的骨质流失。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 DOI: 10.1080/03008207.2023.2199086

Gilberto Li Feng, Marc D Grynpas, Jane Mitchell

{"title":"β-Arrestin 2 knockout prevents bone loss in response to continuous parathyroid hormone stimulation in male and female mice.","authors":"Gilberto Li Feng, Marc D Grynpas, Jane Mitchell","doi":"10.1080/03008207.2023.2199086","DOIUrl":"https://doi.org/10.1080/03008207.2023.2199086","url":null,"abstract":"Background: β-Arrestin 2 (β-arr2) binds activated parathyroid hormone (PTH) receptors stimulating internalization. PTH stimulates both anabolic and catabolic effect on bone depending on the way it is administered. Intermittent PTH stimulation increases trabecular bone formation in mice, but this is decreased in mice lacking β-arr 2, suggesting a role for β-arr 2 in the anabolic effects of PTH. The role of β-arr 2 in the catabolic effects of continuous PTH (cPTH) treatment is not known.Objective: To assess the effects of cPTH administration on bone in mice lacking β-arr 2 compared to wild-type (WT).Methods: Groups of male and female WT or β-arr2 knockout (KO) mice were administered either PTH or phosphate-buffered saline by osmotic pumps for 2 weeks. Following treatment, serum calcium and phosphate levels were measured, bone structure and mineral density were measured by microcomputed tomography, and bone cells measured by static and dynamic histomorphometry.Results: β-arr2 KO had no effects on skeletal development in mice of either sex. PTH treatment caused hypercalcemia and hypophosphatemia and decreased trabecular and cortical bone only in male WT mice. β-arr2 KO in male mice completely abrogated the effects of PTH on bone, while in female β-arr2 KO mice, PTH treatment increased trabecular bone with no effects on cortical bone.Conclusions: These results demonstrate a profound sex effect on skeletal responses to cPTH treatment, suggesting a protective effect of estrogen on bone loss. β-arr2 plays a role in restraining the anabolic effects of PTH in both male and female mice.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9746416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periosteum-derived Micrografts for bone regeneration. 骨膜来源的骨再生微移植物。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-07-01 DOI: 10.1080/03008207.2023.2206489

Basel Mahardawi, Kevin A Tompkins, Nikos Mattheos, Sirida Arunjaroensuk, Atiphan Pimkhaokham

{"title":"Periosteum-derived Micrografts for bone regeneration.","authors":"Basel Mahardawi, Kevin A Tompkins, Nikos Mattheos, Sirida Arunjaroensuk, Atiphan Pimkhaokham","doi":"10.1080/03008207.2023.2206489","DOIUrl":"https://doi.org/10.1080/03008207.2023.2206489","url":null,"abstract":"Bone regeneration is currently one of the most widely researched topics in regenerative medicine. Several bone-grafting materials have been introduced and compared. However, the limitations of the currently available grafts have led researchers to investigate new materials to be used. In contrast, the periosteum performs endogenous bone regeneration as seen in physiological bone fracture repair, and transplanted periosteum has been used to induce bone regeneration in animal models. Although many of the introduced bone grafting materials have not been clinically evaluated, the use of the periosteum for bone regeneration has been documented in several clinical situations. Recently, the Micrograft concept, which was initially used to treat burn patients, where the tissue sample is cut into smaller pieces to expand the area that they can cover, has been applied to oral periosteal tissue for inclusion in scaffolds for bone defect healing, and was evaluated in various clinical bone augmentation procedures. This article first presents a brief overview of some of the commonly used bone grafts and their limitations. Next, it provides background information on the periosteum, including its histology and the cell biology and signaling involved in its osteogenic effect, periosteum-derived Micrografts, their osteogenic potential, and their recent clinical applications for bone augmentation.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9746456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mechanics and differential healing outcomes of small and large defect injuries of the tendon-bone attachment in the rat rotator cuff. 大鼠肩袖肌腱-骨附着体大小缺损损伤的力学及不同愈合效果。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-05-01 Epub Date: 2022-12-16 DOI: 10.1080/03008207.2022.2152334

Anna Lia Sullivan, Ryan C Locke, Rachel K Klink, Connor C Leek, James E Carpenter, Megan L Killian

{"title":"Mechanics and differential healing outcomes of small and large defect injuries of the tendon-bone attachment in the rat rotator cuff.","authors":"Anna Lia Sullivan, Ryan C Locke, Rachel K Klink, Connor C Leek, James E Carpenter, Megan L Killian","doi":"10.1080/03008207.2022.2152334","DOIUrl":"10.1080/03008207.2022.2152334","url":null,"abstract":"Introduction: Rotator cuff tear size affects clinical outcomes following rotator cuff repair and is correlated with the risk of recurrent tendon defects. This study aimed to understand if and how the initial defect size influences the structural and mechanical outcomes of the injured rotator cuff attachment in vivo.Methods: Full-thickness punch injuries of the infraspinatus tendon-bone attachment in Long Evans rats were created to compare differences in healing outcomes between small and large defects. Biomechanical properties, gross morphology, bone remodeling, and cell and tissue morphology were assessed at both 3- and 8-weeks of healing.Results: At the time of injury (no healing), large defects had decreased mechanical properties compared to small defects, and both defect sizes had decreased mechanical properties compared to intact attachments. However, the mechanical properties of the two defect groups were not significantly different from each other after 8-weeks of healing and significantly improved compared to no healing but failed to return to intact levels. Local bone volume at the defect site was higher in large compared to small defects on average and increased from 3- to 8-weeks. In contrast, bone quality decreased from 3- to 8-weeks of healing and these changes were not dependent on defect size. Qualitatively, large defects had increased collagen disorganization and neovascularization compared to small defects.Discussion: In this study, we showed that both large and small defects did not regenerate the mechanical and structural integrity of the intact rat rotator cuff attachment following healing in vivo after 8 weeks of healing.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9493382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teriparatide prevented synovial inflammation and cartilage destruction in mice with DMM. 特立帕肽可预防DMM小鼠滑膜炎症和软骨破坏。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-05-01 DOI: 10.1080/03008207.2022.2157723

Xu Liang, Sen-Rui Li, Xin-Xin Zhang, Shi-Hao He, Shan-Shan Li, Tian-Fang Li

{"title":"Teriparatide prevented synovial inflammation and cartilage destruction in mice with DMM.","authors":"Xu Liang, Sen-Rui Li, Xin-Xin Zhang, Shi-Hao He, Shan-Shan Li, Tian-Fang Li","doi":"10.1080/03008207.2022.2157723","DOIUrl":"https://doi.org/10.1080/03008207.2022.2157723","url":null,"abstract":"Aim: Emerging data have demonstrated that low-grade inflammation in osteoarthritis, a long-held degenerative disease. The inflamed synovium produces various cytokines that induce cartilage destruction and joint pain. A previous study showed that teriparatide, an FDA approved anti-osteoporotic drug, may enhance cartilage repair. Our study focuses on its role in OA synovitis.Materials and methods: Primary mouse articular chondrocytes were used to determine the most potent cytokines involved in OA inflammation and cartilage destruction. A destabilization of the medial meniscus mouse model was established to investigate the effect of teriparatide in OA, particularly, on synovial inflammation and cartilage degradation.Results: In vitro experiments showed that TNF-α was the most potent inducer of cartilage matrix-degrading enzymes, and that teriparatide antagonized the TNF-α of effect. Consistently, articular cartilage samples from TNF-α transgenic mice contained more MMP-13 positive chondrocytes than those from wild type mice. In addition, more type II collagen was cleaved in human OA cartilage than in normal cartilage samples.Conclusions: Teriparatide can prevent synovitis and cartilage degradation by suppressing TNF-α mediated MMP-13 overexpression. Together with its chondroregenerative capability, teriparatide may be the first effective disease modifying osteoarthritis drug.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9487618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Formyl peptide receptors in bone research. 骨研究中的甲酰肽受体。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-05-01 Epub Date: 2022-11-28 DOI: 10.1080/03008207.2022.2149397

Mark A Lantieri, Jose R Perdomo Trejo, Quang Le, Abhijit Dighe, Quanjun Cui, Xinlin Yang

{"title":"Formyl peptide receptors in bone research.","authors":"Mark A Lantieri, Jose R Perdomo Trejo, Quang Le, Abhijit Dighe, Quanjun Cui, Xinlin Yang","doi":"10.1080/03008207.2022.2149397","DOIUrl":"10.1080/03008207.2022.2149397","url":null,"abstract":"Purpose/aim of the study: The formyl peptide receptor (FPR) participates in the immune response, with roles in infection and inflammation. In this review article, we summarize the current literature on these roles before discussing the function of FPRs in the pathogenesis of musculoskeletal disorders including osteoarthritis (OA), degenerative disc disease (DDD), and rheumatoid arthritis (RA). Additionally, we discuss the potential diagnostic and therapeutic roles of FPRs in these domains.Methods: PubMed and Ovid MEDLINE searches were performed from 1965 through March 2022. Keywords included \"FPR, tissue expression, inflammation, infection, musculoskeletal disorder, bone, rheumatoid arthritis, osteoarthritis, degenerative disc disease, mitochondria.\"Results: Sixty-nine studies were included in this review article. FPRs appear to be ubiquitous in the pathogenesis, diagnosis, and treatment of common musculoskeletal disorders. They can potentially be utilized for the earlier diagnosis of OA and DDD. They may be employed with mesenchymal stem cells (MSCs) to reverse OA and DDD pathologies. With anti-inflammatory, anti-osteolytic, and pro-angiogenic functions, they may broaden treatment options in RA.Conclusions: FPRs appear to be heavily involved in the pathogenesis of common musculoskeletal conditions, including arthritis, degenerative disc disease, and rheumatoid arthritis. Furthermore, they demonstrate much promise in the diagnosis and treatment of these conditions. Their roles should continue to be explored.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9689821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of pro-inflammatory interleukins in osteoarthritis: a narrative review. 促炎白细胞介素在骨关节炎中的作用:综述。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-05-01 DOI: 10.1080/03008207.2022.2157270

Asma Inès Sana Jrad, Maha Trad, Wafaa Bzeih, Georges El Hasbani, Imad Uthman

{"title":"Role of pro-inflammatory interleukins in osteoarthritis: a narrative review.","authors":"Asma Inès Sana Jrad, Maha Trad, Wafaa Bzeih, Georges El Hasbani, Imad Uthman","doi":"10.1080/03008207.2022.2157270","DOIUrl":"https://doi.org/10.1080/03008207.2022.2157270","url":null,"abstract":"Purpose: This manuscript will summarize the role of pro-inflammatory cytokines and tackle newly discussed ones within the scope of OA pathogenesis as mentioned in the recent literature. This will allow for a better understanding of the mechanisms behind such a complicated disease.Material and methods: Relevant articles were obtained by searching key terms including \"pro-inflammatory cytokines,\" \"inflammation,\" \"pathophysiology,\" \"cartilage damage,\" and \"OA\" in PubMed and Google Scholar databases. The year ranges set for the selection of the articles was between 2015 -2021. Inclusion criteria was based on the relevance and contribution to the field of the study.Results: Osteoarthritis (OA) has a complex multifactorial pathophysiology which is attributed to molecular and biomechanical changes that disrupt the normal balance of synthesis and degradation of articular cartilage and subchondral bone. Pro-inflammatory cytokines, with their wide range of action and intricate signaling pathways, are the constant subject of new discoveries revolving around this inflammatory disease. The available literature indicates that some of these cytokines such as IL-33, IL-17, IL-6, and IL-22 have a direct relation to cartilage degradation, while others like IL-15, IL-1, IL-7, and IL-34 have an indirect one.Conclusions: Inflammation has an essential role in the manifestation of osteoarthritis clinical events. Specifically, certain cytokines exhibit pro-inflammatory properties that are markedly activated during the course of the disease and notably alter the homeostasis of the joint environment. However, clinical trials and observational studies remain insufficient to navigate the varying nature of this disease in humans.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9487619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2