Connective Tissue Research最新文献_第6页

Biomechanical assessment of gastrocnemii and Achilles tendon using MyotonPRO: in vivo measurements, and preliminary in situ measurements using formalin-fixed tissues. 使用MyotonPRO对腓肠肌和跟腱进行生物力学评估：体内测量和使用福尔马林固定组织的初步原位测量。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2024-01-01 Epub Date: 2024-02-01 DOI: 10.1080/03008207.2023.2267682

Xiyao Shan, Kanae Umemoto, Takuro Ishikawa, Kaori Fukushige, Takao Takeuchi, Munekazu Naito

{"title":"Biomechanical assessment of gastrocnemii and Achilles tendon using MyotonPRO: in vivo measurements, and preliminary in situ measurements using formalin-fixed tissues.","authors":"Xiyao Shan, Kanae Umemoto, Takuro Ishikawa, Kaori Fukushige, Takao Takeuchi, Munekazu Naito","doi":"10.1080/03008207.2023.2267682","DOIUrl":"10.1080/03008207.2023.2267682","url":null,"abstract":"Purpose: This study aims to evaluate the reliability and validity of using MyotonPRO to quantify the mechanical properties of the muscle-tendon unit through in vivo measurements and preliminary in situ measurements using formalin-fixed tissues.Materials and methods: The mechanical properties of gastrocnemii and the Achilles tendon of 12 healthy adults (six males and six females, 34.9 ± 5.8 years) were examined for in vivo test twice within a day and once post-24 hours using MyotonPRO, while nine human cadavers (formalin-fixed, 3 males and 6 females, 89.9 ± 5.1 years) were assessed for preliminary in situ test with identical time schedule to evaluate the within-day and inter-day reliability and validity.Results: In vivo tests had very high within-day (ICC: 0.96-0.99) and inter-day reliability (ICC: 0.83-0.96), while in situ tests (formalin-fixed tissues) showed high within-day (ICC: 0.87-0.99) and inter-day reliability (ICC: 0.76-0.98) for the results of tone and stiffness. There was no significant difference in the stiffness of the free part of the Achilles tendon between in vivo and in situ conditions. The stiffness of the lateral gastrocnemius (r = 0.55, p = 0.018), proximal part of the Achilles tendon (r = 0.56, p = 0.015), and free part of the Achilles tendon (r = 0.47, p = 0.048) before removing the skin was significantly correlated with that after removing the skin condition.Conclusions: The findings of the current study suggest that MyotonPRO is reliable and valid for evaluating tendon stiffness both in vivo and in situ (formalin-fixed tissues).","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"16-25"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41194065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of lncRNA SNHG1 in osteogenic differentiation via miR-497-5p/ HIF1AN axis. lncRNA SNHG1通过miR-497-5p/ HIF1AN轴参与成骨分化的机制。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2024-01-01 Epub Date: 2024-02-01 DOI: 10.1080/03008207.2023.2281321

Yuanyuan Lu, Kaihua Pan, Yunqing Zhang, Jiang Peng, Daning Cao, Xiaoming Li

{"title":"The mechanism of lncRNA SNHG1 in osteogenic differentiation via miR-497-5p/ HIF1AN axis.","authors":"Yuanyuan Lu, Kaihua Pan, Yunqing Zhang, Jiang Peng, Daning Cao, Xiaoming Li","doi":"10.1080/03008207.2023.2281321","DOIUrl":"10.1080/03008207.2023.2281321","url":null,"abstract":"The pivotal role of lncRNAs in osteoporosis progression and development necessitates a comprehensive exploration of the functional and precise molecular mechanisms underlying lncRNA SNHG1's regulation of osteoblast differentiation and calcification. The study involved inducing BMSCs cells to differentiate into osteoblasts, followed by transfections of miR-497-5p inhibitors, pcDNA3.1-SNHG1, sh-HIF1AN, miR-497-5p mimics, and respective negative controls into BMSCs. Quantitative PCR (qPCR) was employed to assess the expression of SNHG1 and miR-497-5p. Western Blotting was conducted to measure the levels of short stature-related transcription factor 2 (RUNX2), osteopontin (OPN), osteocalcin (OCN), and HIF1AN. Alkaline phosphatase (ALP) activity was determined using appropriate assay kits. Calcium nodule staining was performed through Alizarin red staining. Dual luciferase reporter gene assays were executed to validate the interaction between SNHG1 and miR-497-5p, as well as HIF1AN. Throughout osteogenic differentiation, there was a down-regulation of SNHG1 and HIF1AN, in contrast to an elevation in miR-497-5p levels. Direct interactions between miR-497-5p and both SNHG1 and HIF1AN were observed. Notably, SNHG1 exhibited the ability to modulate HIF1AN by influencing miR-497-5p, thereby inhibiting osteogenic differentiation. Functioning as a competitive endogenous RNA, lncRNA SNHG1 exerts an inhibitory influence on osteogenic differentiation via the miR-497-5p/HIF1AN axis. This highlights the potential for lncRNA SNHG1 to emerge as a promising therapeutic target for osteoporosis. The study's findings pave the way for a novel target strategy in the future treatment of osteoporosis.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"63-72"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

State transition and intercellular communication of synovial fibroblasts in response to chronic and acute shoulder injuries unveiled by single-cell transcriptomic analyses 通过单细胞转录组分析揭示滑膜成纤维细胞对慢性和急性肩关节损伤的状态转换和细胞间通信

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-12-13 DOI: 10.1080/03008207.2023.2295322

Jiabao Ju, Mingtai Ma, Yichong Zhang, Zhentao Ding, Jianhai Chen

引用次数: 0

Healing potential of curcumin nanomicelles in cutaneous burn wounds: an in vitro and in vivo study. 姜黄素纳米胶束在皮肤烧伤伤口中的愈合潜力：体外和体内研究。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-07-17 DOI: 10.1080/03008207.2023.2235007

Ahmad Oryan, Esmat Alemzadeh, Soroush Mohammadi

{"title":"Healing potential of curcumin nanomicelles in cutaneous burn wounds: an in vitro and in vivo study.","authors":"Ahmad Oryan, Esmat Alemzadeh, Soroush Mohammadi","doi":"10.1080/03008207.2023.2235007","DOIUrl":"10.1080/03008207.2023.2235007","url":null,"abstract":"Purpose/aim of the study: Curcumin is the active substance of turmeric and has been shown to enhance the healing potential of burn wounds. However, its high hydrophobicity and rapid degradability are great challenges for its clinical applications. The development of new curcumin formulations may provide a potential solution to these issues.Methods and results: In this study, we investigated the use of curcumin nanomicelles for wound dressing and evaluated their effects on fibroblast migration and proliferation in vitro. We found that the application of curcumin nanomicelles to the wounds significantly improved wound contraction and increased the expression of transforming growth factor-1 and basic fibroblast growth factor at day 14 of the healing process. Furthermore, curcumin nanomicelles reduced the expression of interleukin-1 at days 7 and 14 post-wounding. Histopathological analysis revealed that the curcumin nanomicelles-treated burn wounds exhibited more organized granulation tissue, improved angiogenesis, and enhanced re-epithelialization. Additionally, the curcumin treatment led to increased hydroxyproline content and enhanced TGF-β1 expression level in the wounds. The in vitro studies also demonstrated that the curcumin nanomicelles induced proliferation and migration of fibroblasts.Conclusion: Overall, our findings suggest that curcumin nanomicelles can be a promising candidate for the treatment of burn wounds.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"555-568"},"PeriodicalIF":2.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10202817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ultrashort wave diathermy on skin wounds in rabbit ears. 超短波透热疗法对兔耳皮肤损伤的影响。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-08-07 DOI: 10.1080/03008207.2023.2242655

Peng-Peng Huang, Rui Zhang, Xiao-Feng Zhang, Zhi-Tao Xu, Du-Chun Zeng, Feng-Bao Sun, Wen-Jie Zhang

{"title":"Effects of ultrashort wave diathermy on skin wounds in rabbit ears.","authors":"Peng-Peng Huang, Rui Zhang, Xiao-Feng Zhang, Zhi-Tao Xu, Du-Chun Zeng, Feng-Bao Sun, Wen-Jie Zhang","doi":"10.1080/03008207.2023.2242655","DOIUrl":"10.1080/03008207.2023.2242655","url":null,"abstract":"Purpose: Ultrashort wave diathermy (USWD) is commonly used in diseases associated with osteoarticular and soft tissue injuries. However, while accelerating wound healing and preventing joint stiffness, there have been few reports on whether it leads to excessive hypertrophic scarring. The aim was to investigate the effects of different doses of USWD on hypertrophic scars.Materials and methods: A rabbit model of hypertrophic scars was used to determine which dose of USWD reduced scar hyperplasia. The scar thickness was calculated using Sirius red staining. All protein expression levels were determined by western blotting, including fibrosis, collagen deposition, and neoangiogenesis related proteins. Subsequently, flow cytometry and ELISAs were used to determine the proportions of macrophage and inflammatory levels.Results: The wounds with USWD in histopathology showed the dermis was more markedly thickened in the 120 mA group, whereas the wounds with the 60 mA were less raised, comparing with the 0 mA; all detected protein levels were increased significantly, the 120 mA group comparing with the others, including heat shock, fibrosis, and neoangiogenesis, whereas the collagen deposition relative protein levels were decreased, the 60 mA group comparing with Sham group; Finally, in the proportion of macrophages and inflammatory levels the 120 mA group were the highest, and the group Sham was lower than group 60 mA.Conclusions: In hypertrophic scars, the 60 mA USWD could relieve scar formation and inflammatory reactions; however, higher doses could result in opposite consequences.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"569-578"},"PeriodicalIF":2.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10325970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of weight bearing after ACL reconstruction on joint contracture in rats. ACL重建后负重对大鼠关节挛缩的影响。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-07-05 DOI: 10.1080/03008207.2023.2232881

Akinori Kaneguchi, Momoka Hayakawa, Atsuhiro Shimoe, Akira Takahashi, Kaoru Yamaoka, Junya Ozawa

{"title":"The effects of weight bearing after ACL reconstruction on joint contracture in rats.","authors":"Akinori Kaneguchi, Momoka Hayakawa, Atsuhiro Shimoe, Akira Takahashi, Kaoru Yamaoka, Junya Ozawa","doi":"10.1080/03008207.2023.2232881","DOIUrl":"10.1080/03008207.2023.2232881","url":null,"abstract":"Purpose: Joint contractures after anterior cruciate ligament (ACL) reconstruction are a serious problem. Given the uncertain effects of weight bearing after ACL reconstruction on contractures, this study was conducted to examine such effects.Materials and methods: To control the amount of weight bearing, ACL-reconstructed rats were reared with either untreated (small weight bearing; weight bearing during locomotion was 54% of pre-surgery at minimum), hindlimb unloading (non-weight bearing), or sustained morphine administration (large weight bearing; weight bearing during locomotion was maintained at 80% or more of pre-surgery) conditions. Untreated rats were used as controls. Knee extension range of motions (ROMs) before (includes myogenic and arthrogenic factors) and after myotomy (includes arthrogenic factor only) and fibrotic reactions in the joint capsule were assessed 7 and 14 days post-surgery.Results: ACL reconstruction significantly reduced ROMs both before and after myotomy and induced fibrosis in the joint capsule accompanying upregulation of fibrosis-related genes (i.e., type I and III collagens and transforming growth factor-β1) at both time points. Morphine administration increased the ROM before myotomy, but not after myotomy 7 days post-surgery. Unloading after ACL reconstruction improved ROMs both before and after myotomy at both time points. In addition, unloading after ACL reconstruction attenuated fibrotic reactions in the joint capsule.Conclusions: Our results suggest that morphine administration improves myogenic contractures in parallel with an increase in the amount of weight bearing. Unloading after ACL reconstruction is effective in reducing both myogenic and arthrogenic contractures.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"543-554"},"PeriodicalIF":2.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9754277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of LncRNA DICER1-AS1 arrests the cell cycle, inhibits cell proliferation, and induces cell apoptosis by regulating CDC5L nuclear transfer in osteosarcoma. LncRNA DICER1-AS1的敲除通过调节骨肉瘤中CDC5L核转移来阻止细胞周期，抑制细胞增殖，并诱导细胞凋亡。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-06-13 DOI: 10.1080/03008207.2023.2223289

Laihua Fu, Songfeng Xu, Yang Zhou, Jingyang Huang, Jin Qiu, Pengzhou Huang

{"title":"Knockdown of LncRNA DICER1-AS1 arrests the cell cycle, inhibits cell proliferation, and induces cell apoptosis by regulating CDC5L nuclear transfer in osteosarcoma.","authors":"Laihua Fu, Songfeng Xu, Yang Zhou, Jingyang Huang, Jin Qiu, Pengzhou Huang","doi":"10.1080/03008207.2023.2223289","DOIUrl":"10.1080/03008207.2023.2223289","url":null,"abstract":"Background: DICER1-AS1 is reported to promote the progression and disturb the cell cycle in osteosarcoma; however, its mechanism has rarely been studied.Materials and methods: DICER1-AS1 expression levels were evaluated by qPCR and fluorescence in situ hybridization (FISH). The total, nuclear, and cytosolic levels of CDC5L were measured by western blotting and immunofluorescence (IF). Cell proliferation, apoptosis, and cell cycle analyses were conducted using the colony formation, CCK-8 assay, terminal transferase-mediated UTP nick end-labeling kit (TUNEL) assay, and flow cytometry. Levels of cell proliferation-, cell cycle-, and cell apoptosis-related proteins were determined by western blotting. RNA immunoprecipitation (RIP) and RNA pull-down assays were conducted to evaluate the relationship between DICER1-AS1 and CDC5L.Results: LncRNA DICER1-AS1 was highly expressed in samples of osteosarcoma tissue and in osteosarcoma cell lines. DICER1-AS1 knockdown inhibited cell proliferation, promoted cell apoptosis, and disturbed the cell cycle. Moreover, DICER1-AS1 was found to bind with CDC5L, and knockdown of DICER-AS1 inhibited the nuclear transfer of CDC5L. DICER1-AS1 knockdown also reversed the effects of CDC5L overexpression on cell proliferation, apoptosis, and the cell cycle. Moreover, CDC5L inhibition suppressed cell proliferation, promoted cell apoptosis, and disturbed the cell cycle, and those effects were further enhanced by DICER1-AS1 knockdown. Finally, DICER1-AS knockdown inhibited tumor growth and proliferation, and promoted cell apoptosis in vivo.Conclusion: LncRNA DICER1-AS1 knockdown inhibits the nuclear transfer of CDC5L protein, arrests the cell cycle, and induces apoptosis to suppress the development of osteosarcoma. Our results suggest a novel target (DICER1-AS1) for treatment of osteosarcoma.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"519-531"},"PeriodicalIF":2.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9622325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MetaLnc9 facilitates osteogenesis of human bone marrow mesenchymal stem cells by activating the AKT pathway. MetaLnc9通过激活AKT途径促进人骨髓间充质干细胞的成骨。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-07-10 DOI: 10.1080/03008207.2023.2232463

Sijia Chang, Ziyao Zhuang, Chanyuan Jin

{"title":"MetaLnc9 facilitates osteogenesis of human bone marrow mesenchymal stem cells by activating the AKT pathway.","authors":"Sijia Chang, Ziyao Zhuang, Chanyuan Jin","doi":"10.1080/03008207.2023.2232463","DOIUrl":"10.1080/03008207.2023.2232463","url":null,"abstract":"Aim of the study: To investigate the role of MetaLnc9 in the osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs).Materials and methods: We used lentiviruses to knockdown or overexpress MetaLnc9 in hBMSCs. qRT-PCR was employed to determine the mRNA levels of osteogenic-related genes in transfected cells. ALP staining and activity assay, ARS staining and quantification were used to identify the degree of osteogenic differentiation. Ectopic bone formation was conducted to examine the osteogenesis of transfected cells in vivo. AKT pathway activator SC-79 and inhibitor LY294002 were used to validate the relationship between MetaLnc9 and AKT signaling pathway.Results: The expression of MetaLnc9 was significantly upregulated in the osteogenic differentiation of hBMSCs. MetaLnc9 knockdown inhibited the osteogenesis of hBMSCs, whereas overexpression of it promoted the osteogenic differentiation both in vitro and in vivo. Taking a deeper insight, we found that MetaLnc9 enhanced the osteogenic differentiation by activating AKT signaling. The inhibitor of AKT signaling LY294002 could reverse the positive effect on osteogenesis brought by MetaLnc9 overexpression, whereas the activator of AKT signaling SC-79 could reverse the negative effect caused by MetaLnc9 knockdown.Conclusion: Our works uncovered a vital role of MetaLnc9 in osteogenesis via regulating the AKT signaling pathway. [Figure: see text].","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"532-542"},"PeriodicalIF":2.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9767081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-09-01 DOI: 10.1080/03008207.2021.2010945

引用次数: 0

The effect of extracorporeal shock wave on joint capsule fibrosis in rats with knee extension contracture: a preliminary study. 体外冲击波对膝关节挛缩大鼠关节囊纤维化影响的初步研究。

IF 2.9 4区医学

Connective Tissue Research Pub Date : 2023-09-01 DOI: 10.1080/03008207.2023.2217254

Chao Hu, Quan Bing Zhang, Feng Wang, Hua Wang, Yun Zhou

{"title":"The effect of extracorporeal shock wave on joint capsule fibrosis in rats with knee extension contracture: a preliminary study.","authors":"Chao Hu, Quan Bing Zhang, Feng Wang, Hua Wang, Yun Zhou","doi":"10.1080/03008207.2023.2217254","DOIUrl":"https://doi.org/10.1080/03008207.2023.2217254","url":null,"abstract":"The purpose of this study was to observe the therapeutic effect of extracorporeal shock wave (ESW) on extensional joint contracture of knee joint in rats and its mechanism on articular capsule fibrosis. Thirty-two SD rats were randomly divided into blank control, immobilization, natural recovery, and ESW intervention groups. Except for the control group, the left knee joints of other rats were fixed with external fixation brace for 4 weeks when they were fully extended to form joint contracture. The effect of intervention was assessed by evaluating joint contracture, total cell count and collagen deposition in joint capsule, and protein expression levels of TGF-β1, p-Smad2/3, Smad2/3, p-JNK, JNK, I and III collagen in joint capsule. ESW can effectively reduce arthrogenic contracture, improve the histopathological changes of anterior joint capsule, inhibit the high expression of target protein and the excessive activation of TGF-β1/Smad2/3/JNK signal pathway. Inhibition of excessive activation of TGF-β1/Smad2/3/JNK pathway may be one of the potential molecular mechanisms by which extracorporeal shock wave can play a role.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":"64 5","pages":"469-478"},"PeriodicalIF":2.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10429530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0