Gait assessment in a female rat Sprague Dawley model of disc-associated low back pain.

IF 2.8 4区 医学 Q3 CELL BIOLOGY
Fei San Lee,Carlos J Cruz,Kyle D Allen,Rebecca A Wachs
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引用次数: 0

Abstract

PURPOSE Gait disturbances are common in human low back pain (LBP) patients, suggesting potential applicability to rodent LBP models. This study aims to assess the influence of disc-associated LBP on gait in female Sprague Dawley rats and explore the utility of the open-source Gait Analysis Instrumentation and Technology Optimized for Rodents (GAITOR) suite as a potential alternative tool for spontaneous pain assessment in a previously established LBP model. MATERIALS AND METHODS Disc degeneration was surgically induced using a one-level disc scrape injury method, and microcomputed tomography was used to assess disc volume loss. After disc injury, axial hypersensitivity was evaluated using the grip strength assay, and an open field test was used to detect spontaneous pain-like behavior. RESULTS Results demonstrated that injured animals exhibit a significant loss in disc volume and reduced grip strength. Open field test did not detect significant differences in distance traveled between sham and injured animals. Concurrently, animals with injured discs did not display significant gait abnormalities in stance time imbalance, temporal symmetry, spatial symmetry, step width, stride length, and duty factor compared to sham. However, comparisons with reference values of normal gait reported in prior literature reveal that injured animals exhibit mild deviations in forelimb and hindlimb stance time imbalance, forelimb temporal symmetry, and hindlimb spatial symmetry at some time points. CONCLUSIONS This study concludes that the disc injury may have very mild effects on gait in female rats within 9 weeks post-injury and recommends future in depth dynamic gait analysis and longer studies beyond 9 weeks to potentially detect gait.
椎间盘相关性腰痛雌性大鼠 Sprague Dawley 模型的步态评估。
目的人类腰背痛(LBP)患者普遍存在步态障碍,这表明步态障碍有可能适用于啮齿动物腰背痛模型。本研究旨在评估椎间盘相关性腰背痛对雌性 Sprague Dawley 大鼠步态的影响,并探索开源啮齿动物优化步态分析仪器和技术(GAITOR)套件作为自发疼痛评估潜在替代工具在先前建立的腰背痛模型中的实用性。结果表明,受伤动物的椎间盘体积显著减小,握力下降。开阔地测试没有检测到假动物和受伤动物在行走距离上的显著差异。同时,与假动物相比,椎间盘受伤的动物在步态时间不平衡、时间对称性、空间对称性、步幅、步长和占位系数方面没有表现出明显的步态异常。然而,与之前文献报道的正常步态参考值进行比较后发现,受伤动物在某些时间点表现出前肢和后肢站立时间不平衡、前肢时间对称性和后肢空间对称性的轻微偏差。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Connective Tissue Research
Connective Tissue Research 生物-细胞生物学
CiteScore
6.60
自引率
3.40%
发文量
37
审稿时长
2 months
期刊介绍: The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.
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