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Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie最新文献

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[Stimulation by ionizing radiation of the proliferative potential of fibroblasts from children with del(13q14) retinoblastoma]. [电离辐射刺激del(13q14)视网膜母细胞瘤儿童成纤维细胞增殖潜能]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
C Diatloff-Zito, C Turleau, M O Cabanis, A Macieira-Coelho, J de Grouchy
{"title":"[Stimulation by ionizing radiation of the proliferative potential of fibroblasts from children with del(13q14) retinoblastoma].","authors":"C Diatloff-Zito,&nbsp;C Turleau,&nbsp;M O Cabanis,&nbsp;A Macieira-Coelho,&nbsp;J de Grouchy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Skin fibroblasts from normal children and two children with a 13q14 deletion retinoblastoma (Rb) were submitted to fractionated doses of gamma radiations. Irradiation reduced the population doublings in normal fibroblasts and the decline was inversely related to the dose. An increase in population doublings was obtained with one of the Rb cell lines. Foci appeared in the irradiated culture of the other Rb donor. It is suggested that fibroblasts from patients with Rb are able to express some phenotypical properties of transformed cells, perhaps related to factors rendering them more susceptible to carcinogens.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 8","pages":"431-4"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17478869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of thiocyanate ions in saliva by coupling high performance liquid chromatography with specific electrode]. 高效液相色谱-特定电极联用法测定唾液中硫氰酸盐离子
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
F Guyon, M T Couchard
{"title":"[Determination of thiocyanate ions in saliva by coupling high performance liquid chromatography with specific electrode].","authors":"F Guyon,&nbsp;M T Couchard","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An electrochemical method for determination of thiocyanide ions in saliva was established. The procedure involves coupling high performance liquid chromatography (HPLC) on bounded silica gel (in order to eliminate the interference of some ions) with specific electrode. This method is sensitive, precise, rapid and requires only few samples. It offers a great interest in toxicology for the detection of smoking exposure.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 5","pages":"237-40"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17478921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Transfection and expression of the S gene of hepatitis B virus in hepatocytes of adult rats in primary culture]. [原代培养成年大鼠肝细胞中乙型肝炎病毒S基因的转染及表达]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
J F Houssais, N Chenciner
{"title":"[Transfection and expression of the S gene of hepatitis B virus in hepatocytes of adult rats in primary culture].","authors":"J F Houssais,&nbsp;N Chenciner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The feasibility of gene transfer in differentiated mammalian cells in primary culture, has been worked out in isolated adult rat hepatocytes. The calcium phosphate technique has been applied to the hepatocytes after 3 to 5 days of culture. The DNA of a plasmid carrying the gene S of HBV has been used in the transfection experiments. The transcription of the gene S in the transfected hepatocytes, characterized by the synthesis of the gene product (the surface antigen HBs Ag) has been demonstrated by radioimmunoassay and cytologic visualization by immunofluorescence.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 10","pages":"497-500"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17478965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Computed cerebral tomography in vivo in Macaca irus I. Geof. on the neuro-ocular plane. Comparison with man]. 猕猴病毒体内的计算机脑断层扫描[j]。在神经眼平面上。与人比较]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
R Saban, M T Iba Zizen, J Rinjard, K Christov, L Strazielle, E A Cabanis
{"title":"[Computed cerebral tomography in vivo in Macaca irus I. Geof. on the neuro-ocular plane. Comparison with man].","authors":"R Saban,&nbsp;M T Iba Zizen,&nbsp;J Rinjard,&nbsp;K Christov,&nbsp;L Strazielle,&nbsp;E A Cabanis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>C.T. exploration by N.O.P. in vivo of a Macaca's head is compared with man. The N.O.P. was recognized from many works (E.A.C.). It permitted a biometric approach and very precise morphologic study of the brain and the face. For the first time, it allows comparisons between monkey and man, at different ages.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 3","pages":"131-6"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17478969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The neurotrophic factor nerve growth factor (NGF) synthetized by murine L-929 cells is not associated with alpha and gamma subunits]. [小鼠L-929细胞合成的神经营养因子神经生长因子(NGF)与α和γ亚基无关]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
E Dicou, D Wion, P Brachet
{"title":"[The neurotrophic factor nerve growth factor (NGF) synthetized by murine L-929 cells is not associated with alpha and gamma subunits].","authors":"E Dicou,&nbsp;D Wion,&nbsp;P Brachet","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>L-929 cells secrete a neurotrophic factor which is immunologically related to the beta NGF present in the male Mouse submaxillary gland. In order to determine whether L-929 cells also synthesize the alpha and gamma subunits which are associated with the beta NGF in the gland, immunoprecipitations were performed with appropriate antisera. Our results indicate the absence of alpha and gamma subunits in the extracellular medium or in the cell extracts and suggest that the synthesis and secretion of the beta NGF by the L cells does not require these two proteins.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 11","pages":"523-5"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17480939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Inhibition of the evoked liberation of (Met5)enkephalin from slices of globus pallidus in the presence of thiorphan and bestatin]. [硫索芬和贝司他汀对苍白球脑组织(Met5)脑啡肽释放的抑制作用]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
B Giros, S de la Baume, C Gros, J C Schwartz
{"title":"[Inhibition of the evoked liberation of (Met5)enkephalin from slices of globus pallidus in the presence of thiorphan and bestatin].","authors":"B Giros,&nbsp;S de la Baume,&nbsp;C Gros,&nbsp;J C Schwartz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>(Met5) enkephalin released from slices of Rat globus pallidus by 30 mM or 50 mM K+ is hydrolysed to the extent of 85% before reaching the incubation medium. When thiorphan and bestatin, two peptidase inhibitors, are added, this inactivation process is completely prevented but the peptide release from tissues is significantly diminished. The latter effect being reversed in the presence of an opioid antagonist, naloxone, in moderate concentration, the existence of an auto-inhibition process regulating enkephalin release is suggested.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 13","pages":"609-12"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17483499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Production of complement C 3 fraction by rat liver epithelial cells in culture. Stimulating effect of monocyte supernatants from human circulating blood]. [培养大鼠肝上皮细胞补体c3片段的产生。]人循环血液中单核细胞上清液的刺激作用[j]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
M Guiguet, G Mack, M F Exilie, R Truchot, P Padieu
{"title":"[Production of complement C 3 fraction by rat liver epithelial cells in culture. Stimulating effect of monocyte supernatants from human circulating blood].","authors":"M Guiguet,&nbsp;G Mack,&nbsp;M F Exilie,&nbsp;R Truchot,&nbsp;P Padieu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A noticeable increase has been observed in the production of the C 3 component of the complement by Rat liver epithelial cells cultured in vitro in the presence of small quantities of supernatant of human blood mononuclear cells cultured for 72 hrs. with phytohemagglutinin. The physicochemical properties suggest that the stimulating factor(s) is(are) protein(s).</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 12","pages":"547-52"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17483568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Light microscopy identification of alpha-melanotropin cells in the anterior lobe of the hypophysis in Landrace pigs]. [长白猪脑垂体前叶α -嗜黑素细胞的光镜鉴定]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
C Dessy, J Verstegen, M Herlant
{"title":"[Light microscopy identification of alpha-melanotropin cells in the anterior lobe of the hypophysis in Landrace pigs].","authors":"C Dessy,&nbsp;J Verstegen,&nbsp;M Herlant","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"296 17","pages":"819-22"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17373084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Release of hepatitis A virus into the culture medium, during its replication in PLC/PRF/5 cells]. [甲型肝炎病毒在PLC/PRF/5细胞中复制时释放到培养基中]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
J M Crance, R Deloince, C Lechevallier, D Crevat, H Laveran, R Fontanges
{"title":"[Release of hepatitis A virus into the culture medium, during its replication in PLC/PRF/5 cells].","authors":"J M Crance,&nbsp;R Deloince,&nbsp;C Lechevallier,&nbsp;D Crevat,&nbsp;H Laveran,&nbsp;R Fontanges","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hepatitis A virus (HAV), obtained from human feces collected 3 days after the onset of jaundice, was propagated in human hepatocarcinoma cells (PLC/PRF/5). In the course of 5 passages, it was released into the supernatant without cytopathic effect. Released HAV was propagated after inoculation into PLC/PRF/5 cells. Its biophysical properties (buoyant density in CsCl: 1, 33 g/cm3; sedimentation coefficient: 155 S) were similar to those of the infectious particle present in the cells or in the stools of patients.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 3","pages":"111-4"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17379826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Adaptation to diet of mRNA coding for pancreatic amylase and serine proteases in the rat]. [大鼠胰淀粉酶和丝氨酸蛋白酶mRNA编码对饮食的适应]。
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01
C Wicker, G Scheele, A Puigserver
{"title":"[Adaptation to diet of mRNA coding for pancreatic amylase and serine proteases in the rat].","authors":"C Wicker,&nbsp;G Scheele,&nbsp;A Puigserver","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Translation of total pancreatic RNA using the rabbit reticulocyte lysate system allowed us to estimate the relative levels of active mRNA coding for a number of secretory proteins in Rats fed high-carbohydrate or high-protein diets. Quantitative synthesis of secretory proteins in isolated pancreatic lobules was also achieved. Protein synthesis in lobules or as the result of active mRNA translation in a cell-free system was found to give similar results either on a qualitative or quantitative standpoint. We were also able to show that the levels of specific mRNA for pancreatic amylase and serine protease zymogens could directly be related to the amount of carbohydrate or protein in the diet. These findings indicate that nutritional regulation of pancreatic enzyme synthesis occurs through the modulation of levels of specific mRNAs in the acinar cell.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 6","pages":"281-4"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17263056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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