[甲型肝炎病毒在PLC/PRF/5细胞中复制时释放到培养基中]。

Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01

J M Crance, R Deloince, C Lechevallier, D Crevat, H Laveran, R Fontanges

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引用次数: 0

摘要

从黄疸发病3天后收集的人类粪便中获得的甲型肝炎病毒(HAV)在人肝癌细胞(PLC/PRF/5)中繁殖。5代后释放到上清液中，无细胞病变作用。将释放的HAV接种到PLC/PRF/5细胞中进行增殖。其生物物理特性(CsCl中浮力密度:1.33 g/cm3;沉降系数:155 S)与细胞或患者粪便中存在的感染性颗粒相似。

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[Release of hepatitis A virus into the culture medium, during its replication in PLC/PRF/5 cells].

Hepatitis A virus (HAV), obtained from human feces collected 3 days after the onset of jaundice, was propagated in human hepatocarcinoma cells (PLC/PRF/5). In the course of 5 passages, it was released into the supernatant without cytopathic effect. Released HAV was propagated after inoculation into PLC/PRF/5 cells. Its biophysical properties (buoyant density in CsCl: 1, 33 g/cm3; sedimentation coefficient: 155 S) were similar to those of the infectious particle present in the cells or in the stools of patients.

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Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie

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