International Journal of High Dilution Research - ISSN 1982-6206最新文献

{"title":"Homeopathic potencies can regulate the growth and toxicity of Raphidiopsis raciborskii (cyanobacteria) and are traceably by solvatochromic dyes","authors":"Suhan Nowrooz Mohammad, Andreia Adelaide G Pinto, Rodrigo Augusto da Silva, Steven J. Cartwright, Alexander L Tournier, Ivana Barbosa Suffredini, João Sarkis Yunes, L. Bonamin","doi":"10.51910/ijhdr.v22i2.1302","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1302","url":null,"abstract":"Introduction: Cyanobacteria are found worldwide and can produce cyanotoxins. Since homeopathic potencies can modulate toxicity in different biological models, this study proposes to investigate whether cyanobacteria toxins might also be the case. Objectives: a) to investigate if homeopathic potencies can improve the resilience of Artemia franciscana (brine shrimp) embryos to saxitoxin (cyanotoxin) and to control the growth of Raphidiopsis raciborskii in vitro; b) to verify if physicochemical parameters can be used to track homeopathic potencies in seawater or cyanobacteria medium, with special focus on the interaction with solvatochromic dyes. Method: Artemia franciscana cysts were cultivated in seawater in 96-well plates to evaluate the hatching rate, vitality, and gene expression of heat shock proteins (Hsp), after being challenged with R. raciborskii extract containing 2.5 µg/liter of saxitoxin and treated with different homeopathic potencies chosen from a screening process. Cultures of R. raciborskii maintained in ASM-1 medium were equally treated with previously screened homeopathic potencies, and their growth was monitored as a function of time. The physicochemical properties of treated water were evaluated by interacting with solvatochromic dyes and measuring pH, conductivity, and temperature. Results: A lower rate of hatching of Artemia franciscana cysts was observed after treatment with Nitric acidum 6cH (p=0.03), and a complete toxicity reversal with increased Hsp 26 and p26 gene expression was seen after treatment with Isotherapic (R. raciborskii extract) 200cH (p≤0.02). Nitric acidum 200cH and Mercurius solubilis 30cH limited the exponential growth of cyanobacteria (p≤0.003) regardless of their concentration. Coumarin 7 was a marker for Nitric acidum 6cH and Isotherapic (R. raciborskii extract) 200cH, even when inserted into seawater. Nile red was a marker for Nitric acidum 200cH and Mercurius solubilis 30cH, even when inserted into ASM-1 medium (p≤0.05). An increase in pH and endo/exothermal effects of the ASM-1 medium was observed after these treatments (p≤0.007). The physicochemical analyzes were optimized after the previous submission of the samples to a constant unidirectional 2400 Gauss magnetic flow. Conclusion: The Isotherapic 200cH improved Artemia franciscana bioresilience to saxitoxin itself; Nitric acidum 200cH and Mercurius solubilis 30cH limited the growth of R. raciborskii. In all cases, those homeopathic potencies that showed biological effects were traceable in the aqueous medium using solvatochromic dyes, especially when the samples were subjected to the magnetic field.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"102 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Pfeiffer Circular Chromatography as a Qualitative Methodology of Viscum album Homeopathic Mother Tinctures: A Multicentric Collaborative Project Between Brazil and Switzerland","authors":"Cassiana Santos de Oliveira Pignaton, Marilisa Guimarães Lara, João Vitor da Costa Batista, C. Scherr, M. Kokornaczyk, Stephan Baumgartner, Carla Holandino, Osvaldo Freitas","doi":"10.51910/ijhdr.v22i2.1359","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1359","url":null,"abstract":"Viscum album (VA) is a peculiar semiparasitic plant with important role in cancer therapy. The differences in phytochemical composition as well as the influence of host tree in the metabolome of VA subspecies are already described1. Our goal is to use the Pfeiffer Circular Chromatography (PCC) as a qualitative methodology to differentiate V. album mother tinctures (VAMT). The assays were conducted in two countries: Brazil (São Paulo and Rio de Janeiro) and Switzerland (Arlesheim). Three VA subspecies (abietis, album, austriacum) and five host trees (Malus domestica, Quercus sp., Ulmus carpinifolia, Pinus sylvestris, Abies alba) were harvested in Switzerland in summer and winter. The VAMTs were prepared following the Brazilian Homeopathic Pharmacopoeia and the PCC experiments were performed as previously published2. The preliminary analysis and the reproducibility of the three independent assays performed in each experimental set are being evaluated by Image J software. The qualitative standard features will be used to the VAMT differentiation regarding the following PCC characteristics: inner and outer-zones, coloration, size, the presence of radial lines, among others. Besides, the High-Performance Thin Layer Chromatography (HPTLC) was performed as standard quality control methodology using the CAMAG instruments to compare the phytochemical profile of V. album mother tinctures described in the French and German Homeopathic Pharmacopoeias. The samples have been characterized by the compounds retention factor (Rf) compared to the chemical standards (chlorogenic acid, caffeic acid, hyperoside and rutin). They present similar chemical markers when both methodologies were compared but with different intensities between season and host trees. Based on the hypothesis that the PCC patterns could be correlated with the chemical composition of VAMT, this multicentric research project will be useful to propose a low-cost and an easy methodology focused on the quality control parameters of these herbal raw materials.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"107 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation of wild animals near the CRAS lake (Mato Grosso do Sul, Brazil) submitted to homeopathic treatment by a slow dispersion device: a possible indication of environmental balance.","authors":"Mônica Filomena Assis de Souza, Ana Paula Felício, Marco Antonio Cucco, Pedro Henrique De Souza Cucco, Cideli Coelho, L. Bonamin, Claudia Regina Almeida Medina de Araujo, Allysson Favero, Larissa Helen Alcantara da Silva, Karine Bonucielli Brum","doi":"10.51910/ijhdr.v22i2.1349","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1349","url":null,"abstract":"Abstract The environmental imbalance in all its parts (land, plant, animal, man) is one of the most significant challenges of modern times due to the difficulty of a comprehensive, effective, and non-aggressive corrective approach. Due to its biophysical qualities, water seems able to propagate physicochemical signals capable of producing effects on living systems, as recently demonstrated by the method of solvatochromic dyes. For this reason, a slow dispersion device containing a solid base soaked in a homeopathic ingredient was developed to treat animals living in defined ecosystems. This work aimed to evaluate the behavior and clinical status of tapirs (Tapirus terrestris) and capybaras (Hydrochoerus hydrochaeris) that inhabit the neighborhood of a lake subjected to the device's action as indicators of a possible balance of the entire system. The area under study was CRAS (Center for the Recovery of Wild Animals), inserted in an urban forest in Campo Grande - MS, Brazil. The anamnesis carried out with the park managers revealed the occurrence of attacks and mutilations among the animals due to stress and the vast infestation of ticks (Amblyomma cajennense) in wild mammals and their larvae in humans that visited the trails, which caused the closure of the park for school excursions given the risk of spreading zoonoses. The device contained a solid base of hydrocolloid and calcium carbonate soaked in a homeopathic formulation containing Chamomilla 12 cH, Artemisia absinthium 12 cH, Calcarea carbonica 30 cH, Psorinum 12 cH, Sulphur 12 cH, Artemisia lerchiana 12 cH, and Apis mellifica 12 cH, indicated for the treatment of animals whose signs of stress are an increase in ectoparasites and misbehavior. The devices were installed at three points: at the source, 500 meters away from the lake, at the watercourse, 200 meters from the lake, and at the lake itself on 10/08/2021, repeating the placement on 12/10/2021. The animals observed were four tapirs and three capybaras. Fifteen days after the date of the 1st placement of the devices, the manifestation of natural animal behavior was observed, such as teaching puppies to swim, indicating well-being. Twenty days after installation, a gradual decrease in teleogynes (adult forms) and larvae was observed, with the disappearance of ticks in all animals after six months of the device installation without chemical intervention to control the parasites. Park employees who frequent the trails and surroundings report that they have not suffered any more attacks from larvae (so-called “micuins”), even in times of greater occurrence, such as the dry seasons. Thus, it is suggested that installing slow dispersion devices in natural springs, dams, and lakes could be used as a resource for ecological balance, and the clinical observation of resident animals could be a possible indicator of environmental recovery. More studies using this approach are needed to consolidate these findings.   Keywords: Homeopathic complex","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"57 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recovery of fire-damaged “cerrado” area treated with homeopathic preparations in slow dispersion devices – a descriptive study.","authors":"Mônica Filomena Assis de Souza, Ana Paula Felício, Martha Gilka Gutierrez Carrijo, Raquel Garcia Machado, Claudia Regina Macedo Coutinho, Cideli Coelho, Leoni V. Bonamin, Marco Antonio Cucco, Pedro Henrique De Souza Cucco, Karine Bonucielli Brum","doi":"10.51910/ijhdr.v22i2.1351","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1351","url":null,"abstract":"Given the seasonal climatic characteristics, forest fires in “cerrado” areas in Central Brazil are common and challenging to prevent. Despite fire being a plant stimulation factor, such as breaking seed dormancy, when it covers large areas or occurs very frequently, it can cause irreparable damage to nature. Prevention is difficult and costly, and there are no corrective action options other than waiting for natural regeneration, which is usually slow and, not infrequently, with permanent damage. Due to its physicochemical qualities acting in biological regulation processes, water has been considered the primary vehicle for propagating signals from homeopathic ingredients, as suggested by previous studies carried out with solvatochromic dyes. Therefore, such inputs could, in theory, be inserted into watercourses to stimulate the regeneration of the biome destroyed by fire. This hypothesis motivated this case study. A slow dispersion device was developed aiming at promoting continuous environmental regeneration, containing hydrocolloid and calcium carbonate as a solid base soaked in a homeopathic complex specifically designed for this purpose, composed of Arsenicum album, Arnica montana, Staphysagria, Ignatia amara, and Phosphorus, all at 30cH. The case occurred in Nascentes do Rio Taquari Park, between Mato Grosso and Mato Grosso do Sul state, Brazil. It is a “cerrado” area, with multiple springs that feed the Paraguay River, occupying an area of 26,849 hectares over the Guarani and Bauru aquifers. After the fire in early September 2020, the devices were fixed at 9 strategic points in the park (P1 to P9) over 10 days, between September 29, and October 11, 2020, in water courses close to the main springs. To assess the restoration signs of the post-fire environment, the technicians responsible for monitoring the park made observations of flora and fauna recomposition in different locations close to four device-insertion points (P3, P5, P7, P8). Signs of recovery were observed 40 days after the fire was over. A rapid pioneer plant restructuring was noted, with a significant regrowth of grass, herbaceous and shrub species, such as Mutamba (Guazuma ulmifolia), Murici (Byrsonima spp.), Inga (Inga sp.), Brachiaria (Brachiaria sp.), Jaraguá grass (Hyparrhenia rufa), Colonião grass (Panicum maximum), Gabiroba (Campomanesia sp.), and Pixirica (Miconia sp.). Some species, such as Mimosa (Mimosa sp.), Colonião grass (Panicum maximum), and Jaraguá grass (Hyparrhenia rufa), were not detected in the area before the fire, probably by the seed bank stimulation caused by the heat. There was rapid forest regeneration (4 months after the fire) and restoration of most of the burned trees, both for resisting the fire and for being free of invasive species highly aggressive to native plants, which were controlled by the action of fire. Concerning the fauna, a vast animal population was detected, especially birds, highlighting the “Tuiuiú” (Jabiru mycteria) and “Socó” ","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"161 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Need for external replication trials in homeopathic basic research: presenting research on three duckweed test systems as an example","authors":"Annekathrin Ücker","doi":"10.51910/ijhdr.v22i2.1354","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1354","url":null,"abstract":"One of the main aims in homeopathic basic research is to find reliable test systems that are externally replicable. In face of the replication crisis in research in general and the specificities that are necessary to include in homeopathic basic research, it is of great importance to encourage high-quality replication trials. One way to achieve that is to build up a register or a data base for test systems in homeopathic basic research that are already developed to the point that external replication trials are possible. Such a register offers the opportunity to include not just the published information but also helpful insight such as material lists and time frames for easier planning and conduction of replication trials. Additionally, further information on critical environmental parameter, unpublished negative results, etc. could be included. The general frame work of such a register/ data base is demonstrated with the example of three different duckweed test systems developed in our working group since 2001. The growth rate of duckweed (Lemna gibba L., clone number 9352) was investigated in randomised and blinded settings, using either untreated plants or plants pre-treated with a stressor (arsenic solution or calcium deficiency in the growth medium). Within the original trials, potentised substances in the range of 14x-33x were compared to water control groups (succussed and unsuccussed water). The  stability of the test systems was investigated in parallel systematic negative control (SNC) experiments. All original trials revealed a significant influence of some potencies on the growth rate of duckweed and stable test systems as shown with SNC experiments. Four internal replication trials for two of the three test systems were conducted. The replication trials showed effects of homeopathic preparations with some differences in effect size and effect direction compared to original studies.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"180 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Is the Vital Force? Towards a Quantum Perspective on Vitalism and Possible Consequences for CAM.","authors":"Lionel Milgrom","doi":"10.51910/ijhdr.v22i2.1298","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1298","url":null,"abstract":"A brief history of vitalism is followed by a description of the scientific paradigm and the relationship of information to quantum theory. Quantum Bayesianism (QBism) is introduced as an alternative subjective interpretation of quantum theory, and a better descriptor of the therapeutic process (TP) than previous objective conventional quantum theoretical (CQT) interpretations. The vital force (Vf) is considered a system defined solely by observations of a patient’s (Px’s) secondary symptoms (S2) during case-taking, and changes to them (DS2) after treatment. This is combined with a previous gyroscopic metaphor of the Vf. According to QBism, wave functions generated by this metaphor depend on subjective degrees of belief in observations of S2 and DS2. As such, assessing a Px’s S2 and DS2 not only changes over time but may well differ from another’s assessment of the same Px. Conclusion: Treating the Vf as an analogue of the quantum state of a macroscopic system, i.e., Px, represents all that can possibly be known about a Px’s symptoms by observation, not the Vf’s presumed reality, separate from an observer. The Vf is simply a representation of knowledge about Px, suggesting how a subjective generally valid quantum metaphor for the TP might develop in future.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"25 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature and structure of NTA Traditional Homeopathically Manufactured Medicines.","authors":"M. Wassenhoven, Martine Goyens, Pierre Dorfman","doi":"10.51910/ijhdr.v22i2.1334","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1334","url":null,"abstract":"Background: Research using several different methods has demonstrated the materiality, including nanoparticles (NPs) and molecular shapes, in High Homeopathic Potentizations (HHPs). This presentation is a summary of all the evidence gathered by the DynHom research project. Methods: We used the Nuclear Magnetic Resonance (NMR), Nano Tracking Analyzer (NTA), Electronic microscopy (SEM-EDX), Infrared spectroscopy (FTIR), pH measurements to examine dilutions of commonly used homeopathic medicines and controls. The homeopathic medicines tested were specially prepared according to the European pharmacopoeia standards. We compared the homeopathic dilutions/dynamizations with simple dilutions and solvents controls. Results: We observed specific NMR profiles, the presence of particles with specific size distribution and electric fields, dry material (crystals) with a specific molecular structure even more obvious in HHPs. Conclusion: Homeopathic medicines do contain particles with a specific size distribution even in HHPs diluted beyond the Avogadro/Loschmidt limit. They also contain molecules in particular concentrations according to each source material. This specificity can be attributed to the manufacturing and potentization process. This material demonstrates that the step-by-step process (dynamized or not) does not match the theoretical expectations in a dilution process. The starting material, the type of containers and dilution/dynamization method influence the nature and concentration of these NPs.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"37 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homeopathy in the Treatment of Temporomandibular Disorders in Covid-19","authors":"R. Nardy, Vinícius Henrique Nascimento, Leonardo Dos Santos Barroso, Adriana Marques Nunes, Leila Dos Santos Mourão","doi":"10.51910/ijhdr.v22i2.1307","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1307","url":null,"abstract":"ABSTRACT The literature records the relevance of emotional and psychosocial status as triggering factors for sleep disorders and orofacial pain, including temporomandibular disorder. Homeopathy is a complementary treatment method for these conditions. SARS-CoV-2 is a virus that causes respiratory disease, COVID-19, identified in December 2019, in China the significant increase in changes in sleep and mood cycles as triggers of various forms of orofacial pain and bruxism, presented as oral-facial comorbidities resulting from the pandemic. The objective of this retrospective observational study was to analyze data from 129 records of patients seen between May 2020 and May 2021 diagnosed with Temporomandibular Disorder and seen at Clínica Núcleo Endo for dentistry and orofacial pain care. Methodology: Of these, 35 with a diagnosis of TMD and clinical follow-up by a Pittsburgh sleep quality questionnaire and questionnaire, received a prescription for the homeopathic medicine Chamomille 30CH (Matricaria chamomilla L.) drops at a dose of 5 drops daily to assess the effectiveness of the use of the drug in controlling pain and changes in sleep and mood, with and without the installation of the interocclusal plaque. Conclusion: Chamomilla 30 CH has been demonstrated to be effective as a treatment Complement the changes: Of pain, stress and anxiety and humor. Other clinical work are necessary for further elucidation About chamomilla's action mechanisms and its healing potential.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"452 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cantharis potency acts on heat exposed bovine serum albumin as revealed by FT-IR spectroscopy","authors":"N. Sukul, Raj Kumar Singh, N. Pande, A. Sukul","doi":"10.51910/ijhdr.v22i2.1343","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1343","url":null,"abstract":"In a series of experiments we have demonstrated that high dilution of homeopathic drugs produces distinct effects on plants and animals in-vivo through the medium of water. High dilutions (HDs) of a drug prepared by serial dilution with a solvent medium 1:100, followed by mechanical agitation in several steps are called potencies. Water molecules play an important role on protein structure and function. We focused our observation on water molecules associated with BSA. High dilution (HD) of a homeopathic drug is thought to be specific water structure which produces therapeutic effects on man. HDs also produce effects on animals, plants and even proteins in aqueous solution. What is the primary target of HDs on organisms or on protein? The present study addresses this question. Cantharis 30 cH, a homeopathic potency used for burn injuries, has been tested on bovine serum albumin (BSA) in-vitro. The protein has been exposed to heat at 750C for 10 mins, cooled down to room temperature and then treated with Cantharis 30 cH. The treatment was done by two methods, direct and indirect through water. The results from both the methods are almost similar.  The treated and untreated protein solutions were analysed by Fourier Transform Infra-Red (FT-IR) spectroscopy. The treatment effect was evident on water molecules associated with BSA. The results indicate that Cantharis 30 cH interacts with water molecules which in turn influence BSA. It is concluded that the homeopathic potency converts first water structure which then affects the protein. Based on the results on this experiment we can assumes that the primary target of all homeopathic potencies is water which constitutes the major component of all living organisms. All biomolecules are suspended in water.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"54 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of mastitis before and after homeopathic treatment in dairy cattle, in the state of Rio de Janeiro, Brazil.","authors":"Gleyce Moreno Barbosa, Rossiane De Moura Souza, Jeniffer Ferreira de Miranda, Guilherme Castro Luz da Silva, Thalisson Portela Xavier Medeiros","doi":"10.51910/ijhdr.v22i2.1363","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1363","url":null,"abstract":"Bovine mastitis is an infectious disease that impacts milk production, leads to economic losses and public health problems. It can present in subclinical and clinical forms, with subclinical being the most common. Currently, there is concern about the health of dairy cattle and alternatives are being sought for the treatment of bacterial infections, as those available may have low efficacy, promote antimicrobial resistance and/or have toxic effects. From a One Health perspective, multiple sectors must work together to control the rise in antimicrobial resistance. Regarding animal production, this implies changes in some traditional agricultural practices (WHO, 2022; OIE, 2022). According to Rabello et al. (2020), resistance to antimicrobials for Staphylococcus aureus isolated from bovine mastitis has recently increased compared to studies published in previous periods, in Brazil. In this context, homeopathy can represent an important therapy. In our study, two dairy herds in the state of Rio de Janeiro were evaluated, regarding the presence of mastitis, in two subsequent periods: 1- Control Stage (CS): using antibiotic therapy, and 2- Experimental Stage (ES): homeopathic treatment (Nosode 30CH). The CS took place from February to April 2022 and the ES from May to July 2022. For the analysis of the treatments, the animals were evaluated with the California Mastitis Test (CMT) and the milk from the positive mammary quarters, that presented mastitis, were collected and analyzed in the laboratory for bacterial isolation and identification. During the CS, 7 CMT were performed, and the same number in the ES. The occurrence of subclinical mastitis in the CS in 1681 analyzed quarters was 36.4% (n= 608), while in the ES, in 1290 analyzed quarters, subclinical mastitis was 26.5% (n= 335). There was a reduction (p<0.0001) in the occurrence of subclinical mastitis after homeopathic treatment. In both stages Staphylococcus spp. was the most prevalent microorganism.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"10 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}