Toxicology Research最新文献_第10页

Investigation of the toxicity and safety concerns of transgenic maize seeds expressing immunogenic F and HN protein genes against Newcastle disease virus. 调查表达抗新城疫病毒免疫原性 F 和 HN 蛋白基因的转基因玉米种子的毒性和安全问题。

IF 2.1 4区医学

Toxicology Research Pub Date : 2024-09-17 DOI: 10.1093/toxres/tfae143

Muhammad Saad Bhutta,Naila Shahid,Sara Ajmal,Sana Shakoor,Zainab Khursheed,Ibrahim B Salisu,Sheraz Ahmad,Saira Azam,Aneela Yasmeen,Ayesha Latif,Abdul Qayyum Rao

{"title":"Investigation of the toxicity and safety concerns of transgenic maize seeds expressing immunogenic F and HN protein genes against Newcastle disease virus.","authors":"Muhammad Saad Bhutta,Naila Shahid,Sara Ajmal,Sana Shakoor,Zainab Khursheed,Ibrahim B Salisu,Sheraz Ahmad,Saira Azam,Aneela Yasmeen,Ayesha Latif,Abdul Qayyum Rao","doi":"10.1093/toxres/tfae143","DOIUrl":"https://doi.org/10.1093/toxres/tfae143","url":null,"abstract":"IntroductionThe presented study investigated the potential toxicity and safety concerns associated with transgenic maize seeds expressing immunogenic F and HN protein genes against Newcastle disease virus (NDV).MethodologyThe experiment involved feeding Sprague-Dawley rats with transgenic maize seeds formulated into standard diets at levels of 30% (w/w) for a duration of 90 days. The rats were divided into three groups, with 10 rats per group. We assessed various parameters including overall appearance, feed consumption, body weight, organ weight, hematological parameters, serum chemistry, and histopathology.ResultsThe results of these assessments were compared between the control group and the treatment groups. The study findings revealed that there were no significant differences between the control and treatment groups in terms of overall appearance, feed consumption, body weight, organ weight, hematological parameters, serum chemistry, microscopic histopathology, and gross appearance of tissues. These observations suggest that the consumption of transgenic maize seeds did not lead to any treatment-related adverse effects or toxicological issues. Furthermore, the transgenic maize seeds were found to be nutritionally equivalent to their non-transgenic counterpart.ConclusionOverall, no physiological, pathological, or molecular toxicity was observed in the Rats fed with transgenic feed.However, it is important to note that this study focused specifically on the parameters measured and the outcomes observed in Sprague-Dawley rats, and further research and studies are necessary to fully evaluate the safety and potential applications of transgenic edible vaccines in humans or other animals.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"6 1","pages":"tfae143"},"PeriodicalIF":2.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterile inflammation induced by respirable micro and nano polystyrene particles in the pathogenesis of pulmonary diseases. 可吸入微型和纳米聚苯乙烯颗粒在肺部疾病发病机制中诱发的无菌炎症。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-09-02 eCollection Date: 2024-10-01 DOI: 10.1093/toxres/tfae138

Laganà Antonio, Giuseppa Visalli, Alessio Facciolà, Caterina Saija, Maria Paola Bertuccio, Barbara Baluce, Consuelo Celesti, Daniela Iannazzo, Angela Di Pietro

{"title":"Sterile inflammation induced by respirable micro and nano polystyrene particles in the pathogenesis of pulmonary diseases.","authors":"Laganà Antonio, Giuseppa Visalli, Alessio Facciolà, Caterina Saija, Maria Paola Bertuccio, Barbara Baluce, Consuelo Celesti, Daniela Iannazzo, Angela Di Pietro","doi":"10.1093/toxres/tfae138","DOIUrl":"10.1093/toxres/tfae138","url":null,"abstract":"Sterile inflammation is involved in the lung pathogenesis induced by respirable particles, including micro- and nanoplastics. Their increasing amounts in the ambient and in indoor air pose a risk to human health. In two human cell lines (A549 and THP-1) we assessed the proinflammatory behavior of polystyrene nanoplastics (nPS) and microplastics (mPS) (Ø 0.1 and 1 μm). Reproducing environmental aging, in addition to virgin, the cells were exposed to oxidized nPS/mPS. To study the response of the monocytes to the inflammatory signal transmitted by the A549 through the release of soluble factors (e.g. alarmins and cytokines), THP-1 cells were also exposed to the supernatants of previously nPS/mPS-treated A549. After dynamic-light-scattering (DLS) analysis and protein measurements for the assessment of protein corona in nPS/mPS, real-time PCR and enzyme-linked-immunosorbent (ELISA) assays were performed in exposed cells. The pro-inflammatory effects of v- and ox-nPS/mPS were attested by the imbalance of the Bax/Bcl-2 ratio in A549, which was able to trigger the inflammatory cascade, inhibiting the immunologically silent apoptosis. The involvement of NFkB was confirmed by the overexpression of p65 after exposure to ox-nPS and v- and ox-mPS. The fast and higher levels of IL-1β, only in THP-1 cells, underlined the NLPR3 inflammasome activation.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 5","pages":"tfae138"},"PeriodicalIF":2.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on the regulatory mechanism of cell senescence in arsenic toxicity: a systematic review. 砷中毒细胞衰老调控机制的研究进展：系统综述。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-22 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae136

Yun Gu, Ying Qiu, Yujian Li, Weihua Wen

引用次数: 0

Assessment of cytoprotective and genoprotective effects of Moringa oleifera and Tinospora cordifolia extracts in vitro. 在体外评估辣木和虎杖提取物的细胞保护和基因保护作用。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-22 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae133

Preeti Bagri, Vinod Kumar, Kanisht Batra

{"title":"Assessment of cytoprotective and genoprotective effects of Moringa oleifera and Tinospora cordifolia extracts in vitro.","authors":"Preeti Bagri, Vinod Kumar, Kanisht Batra","doi":"10.1093/toxres/tfae133","DOIUrl":"10.1093/toxres/tfae133","url":null,"abstract":"Background: Moringa oleifera and Tinospora cordifolia is extensively used as an ingredient of food and in traditional medicine for the management of a variety of diseases.Material and methods: The extracts of leaf of Moringa oleifera and stem of Tinospora cordifolia were assessed to examine their ability to inhibit the oxidative DNA damage (by DNA protection assay), cytoprotective and genoprotective potential (by Comet assay) in V79 cells individually and in combinations.Result: It was found that these extracts could significantly inhibit the OH-dependent damage of pUC18 plasmid DNA. M. oleifera extract (160 and 320 μg/mL) and Tinospora cordifolia extract (640, 1,280 and 2,560 μg/mL) individually showed higher DNA protection activity. M. oleifera (1,280 μg/mL) combined with Tinospora cordifolia (640 μg/mL) showed best cytoprotective and genoprotective activities among different concentration combinations and various concentrations of individual plants in V79 cell line against hydrogen peroxide induced cytotoxicity and genotoxicity.Conclusion: This study demonstrates the cytoprotective and genoprotective activity of M. oleifera and Tinospora cordifolia individually or in combination.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae133"},"PeriodicalIF":2.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11339162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low expression of Lnc-ENST00000535078 inhibits the migration, invasion, and enhances apoptosis of CTPE-induced malignantly transformed BEAS-2B cells. 低表达 Lnc-ENST00000535078 可抑制 CTPE 诱导的恶性转化 BEAS-2B 细胞的迁移、侵袭并增强其凋亡。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-21 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae121

Ping Lu, Liu Yang, Yanting Lei, Yuezeng Zhao, Zhihao Tang, Pingping Shang, Xiaolei Zhou, Pengpeng Wang, Wei Wang, Feifei Feng, Qiao Zhang

{"title":"Low expression of Lnc-ENST00000535078 inhibits the migration, invasion, and enhances apoptosis of CTPE-induced malignantly transformed BEAS-2B cells.","authors":"Ping Lu, Liu Yang, Yanting Lei, Yuezeng Zhao, Zhihao Tang, Pingping Shang, Xiaolei Zhou, Pengpeng Wang, Wei Wang, Feifei Feng, Qiao Zhang","doi":"10.1093/toxres/tfae121","DOIUrl":"10.1093/toxres/tfae121","url":null,"abstract":"Long non-coding RNA (LncRNA) plays an important role in malignant transformation of cells. This study aimed to explore the role of Lnc-ENST00000535078 in the malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) induced by coal tar pitch extract (CTPE). The malignant transformation model of BEAS-2B cells exposed to CTPE. Cell proliferation was examined by Cell counting kit-8 (CCK-8) assay. Colony formation assay was used to assess the colony of cells. Cell migration and invasion were detected by Transwell analysis. Cell cycle progression and apoptotic status were assessed by flow cytometry. Differentially expressed genes were screened by RNA sequencing. The results showed that Lnc-ENST00000535078 expression was highest in malignantly transformed BEAS-2B cells passaged to the 30th generation. Knockdown of Lnc-ENST00000535078 inhibited the migration, invasion and anti-apoptotic abilities of malignantly transformed BEAS-2B cells. Transcriptome analysis found 608 differential genes. CCND1 and FOS genes were screened out because of their levels were positive correlation with the expression of Lnc-ENST00000535078, which were consistent with the RNA sequencing results. In conclusion, Low expression of Lnc-ENST00000535078 inhibits the migration and invasion of malignant transformed BEAS-2B cells and promotes apoptosis in these cells. Lnc-ENST00000556926 might affect the malignant transformation of cells through the regulation of CCND1 and FOS. This study may provide a potential target for intervention in CTPE-induced lung cancer.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae121"},"PeriodicalIF":2.2,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11336064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative impacts of Sinapic acid against monosodium urate crystal-induced gouty arthritis and inflammation through different signaling pathways. 西那皮酸通过不同的信号通路对单钠尿酸盐晶体诱发的痛风性关节炎和炎症有改善作用。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-21 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae130

Muhammad Ishaq, Liang Zhao, Mohamed Mohamed Soliman, Saed Althobaiti, Helal F Al-Harthi, Shatha B Albattal, Wang Chengtao

{"title":"Ameliorative impacts of Sinapic acid against monosodium urate crystal-induced gouty arthritis and inflammation through different signaling pathways.","authors":"Muhammad Ishaq, Liang Zhao, Mohamed Mohamed Soliman, Saed Althobaiti, Helal F Al-Harthi, Shatha B Albattal, Wang Chengtao","doi":"10.1093/toxres/tfae130","DOIUrl":"10.1093/toxres/tfae130","url":null,"abstract":"As known, gout a metabolic disease due to the urate crystals deposition in the joints and affect human health and state. Humans are looking for safe natural remedies from plants with safe, low cost and high effect on their health. Sinapic acid (SA) is found in plants and used as phytoconstituent in human diets. SA has strong antioxidant activity, bone-regenerative, anti-cancer, anti-allergic, and antidiabetic effects. The current study was outlined to confirm the anti-gout potential of SA against monosodium urate crystals (MSU)-induced gouty arthritis in mice. Positive gouty arthritis was conducted by administration of colchicine and MSU in the hind paw. SA was orally administered to negative and positive MSU arthritic mice at 25 and 50 mg/kg, one-hour before MSU injection (100 μg/kg intra-articular). At the end of the experiment, sampling was done for serum, histopathology, oxidative stress and gene expression analysis. The results showed that SA significantly recovered the joint edema and recovered MSU crystals-showed histopathological changes. The production of cytokines, leukocyte recruitment, oxidative stress, and nucleotide-binding domain, leucinerich-containing family, pyrin domain-containing-3 (NLRP3)-inflammasome genes expressions were increased in positive arthritic mice and ameliorated significantly by SA administration. Moreover, SA showed ameliorative impacts on air pouch model of mice as reported by the down regulation in the expression of inflammation related blood cells, proinflammatory cytokines and other transcriptional genes. In conclusion, sinapic acid showed a potential therapeutic use against side effects accompanying gouty arthritis and is good as a supplement against inflammation associated disorders.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae130"},"PeriodicalIF":2.2,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11336067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orlistat and metformin combination ameliorates obesity-induced renal injury via suppressing renal oxidative stress in male rats. 奥利司他和二甲双胍复方制剂通过抑制雄性大鼠肾脏氧化应激改善肥胖引起的肾损伤

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-21 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae135

Khadeejah Alsolami, Reham Z Hamza

{"title":"Orlistat and metformin combination ameliorates obesity-induced renal injury via suppressing renal oxidative stress in male rats.","authors":"Khadeejah Alsolami, Reham Z Hamza","doi":"10.1093/toxres/tfae135","DOIUrl":"10.1093/toxres/tfae135","url":null,"abstract":"Background: Orlistat (ORS) and metformin (MEF) are robustly used as well-established clinical drugs for the treatment for both obesity and the consequences of diabetes mellitus. Additionally, no study has been conducted to explore the consequence of the combination of both ORS and MEF on the kidneys of rats with obesity-induced renal injury (OBS).Objectives: Therefore, the objective of the current research was designed to explore the possible ameliorative effects of either ORS and/or MEF or their combination against obesity (OBS) induced experimental renal oxidative stress.Methods: Renal oxidative stress was investigated at redox histopathological and immunohistological points in the kidney tissues.Results: The levels of urea, uric acid, and creatinine increased with the obesity effect; in addition, the myeloperoxidase (MPO) and xanthine oxidase (XO) activators were elevated significantly with the induction of OBS. The levels of non-enzymatic antioxidants (glutathione and thiol) declined sharply in OBS rats as compared to the normal group.Conclusion: The data displayed that the combination of both ORS and MEF declined the obesity effects significantly by reducing the level of peroxidation (MDA), and enhancement intracellular antioxidant enzymes. These biochemical findings were supported by histopathology, immunohistochemistry, and Masson-Trichrome evaluation, which showed minor morphological changes in the kidneys of rats.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae135"},"PeriodicalIF":2.2,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11336066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-oxidant activity of coenzyme Q10 against AlCl₃/D-galactose in albino rat induced cognitive dysfunctions: Behavioral, biochemical, and BACE-1/GSK-3β alterations. 辅酶Q10对AlCl3/D-半乳糖诱导的白化大鼠认知功能障碍的抗氧化活性：行为、生化和 BACE-1/GSK-3β 改变。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-19 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae131

Nagat Fawzy Nawar, Doha Mohammad Beltagy, Tarek Mostafa Mohamed, Ehab Mostafa Tousson, Mai Mahmoud El-Keey

{"title":"Anti-oxidant activity of coenzyme Q10 against AlCl3/D-galactose in albino rat induced cognitive dysfunctions: Behavioral, biochemical, and BACE-1/GSK-3β alterations.","authors":"Nagat Fawzy Nawar, Doha Mohammad Beltagy, Tarek Mostafa Mohamed, Ehab Mostafa Tousson, Mai Mahmoud El-Keey","doi":"10.1093/toxres/tfae131","DOIUrl":"10.1093/toxres/tfae131","url":null,"abstract":"The relationship between amyloid beta (Aβ) and oxidative stress (OS), both prominent factors in Alzheimer's disease-related neural degeneration, is deeply interconnected. The cleavage of the extracellular domain of Amyloid precursor protein (APP) and phosphorylating different substrates, respectively, the β-site amyloid precursor protein cleaving enzyme-1 (BACE-1) and Glycogen synthase kinase-3-beta (GSK-3β) enzymes initiate the synthesis of Aβ, which causes cognitive deficits in AD. This study aimed to explore the protective potential of Coenzyme Q10 (CoQ10). It also sought to uncover any synergistic effects when combined with donepezil, an acetylcholinesterase inhibitor, in treating Alzheimer's disease in male albino rats, focusing on the modulation of the BACE-1/GSK-3β pathway. The experiment involved 70 rats categorized into different groups: control, donepezil alone, CoQ10 alone, AD-model, donepezil co-treatment, CoQ10 co-treatment, and CoQ10 + donepezil combination. Various assessments, such as cholinesterase activity, oxidative stress, serum iron profile, Brain Derived Neurotrophic Factor (BDNF), Tau protein, β-site amyloid precursor protein cleaving enzyme-1 (BACE-1), phosphatase and tensin homolog (Pten), and Glycogen synthase kinase-3-beta (GSK-3β), were conducted on behavioral and biochemical aspects. CoQ10 treatment demonstrated memory improvement, enhanced locomotion, and increased neuronal differentiation, mainly through the inhibition of the dual BACE-1/GSK-3β. These findings were substantiated by histological and immunohistological examinations of the hippocampus.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae131"},"PeriodicalIF":2.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing CircHIPK3 improves sevoflurane-explore learning and memory dysfunction and nerve damage via enhancing miR-338-3p. 沉默CircHIPK3可通过增强miR-338-3p改善七氟醚探索中的学习和记忆功能障碍以及神经损伤。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-19 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae132

Xiuli Li, Xuefei Li, Yinan Liang

{"title":"Silencing CircHIPK3 improves sevoflurane-explore learning and memory dysfunction and nerve damage via enhancing miR-338-3p.","authors":"Xiuli Li, Xuefei Li, Yinan Liang","doi":"10.1093/toxres/tfae132","DOIUrl":"10.1093/toxres/tfae132","url":null,"abstract":"Background: Sevoflurane (Sev), a widely used volatile anesthetic, can cause neurotoxicity, and impair learning and memory.Objective: This study investigates the role and mechanisms of circHIPK3 in Sev-exposed neurotoxicity and learning and memory impairment.Methods: SD rats and hippocampal neuronal cells were exposed to Sev. RT-qPCR analysis of circHIPK3 and miR-338-3p levels. MWM test was performed to examine the behavioral changes in rats. The levels of circHIPK3 and miR-338-3p levels were investigated using RT-qPCR. ELISA assay to analyze the expression of pro-inflammatory factors. CCK-8, flow cytometry, and commercial ROS assay kits were analyzed to detect cell viability, apoptosis, and ROS production. DLR and RIP assays validate circHIPK3 binding to miR-338-3p.Results: Sev increased circHIPK3 expression in rat hippocampal tissue as well as in neuronal cells but decreased miR-338-3p levels compared to controls. circHIPK3 binding to miR-338-3p. Furthermore, silencing of circHIPK3 rats attenuated Sev-induced decline in learning and memory functions . silencing circHIPK3 also reduced Sev-induced secretion of inflammatory factors in rat and neuronal cells. Reducing circHIPK3 partially reversed the Sev-induced decrease in cell viability, increased apoptosis, and overproduction of ROS. However, the inhibitory effect of circHIPK3 on Sev neurotoxicity was restored upon downregulation of miR-338-3p.Conclusion: Collectively, silencing circHIPK3 alleviates Sev exposure-induced learning and memory deficits and neurotoxicity by enhancing miR-338-3p expression.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae132"},"PeriodicalIF":2.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnolin alleviates cyclophosphamide-induced oxidative stress, inflammation, and apoptosis via Nrf2/HO-1 signaling pathway. 木兰脂素可通过Nrf2/HO-1信号通路减轻环磷酰胺诱导的氧化应激、炎症和细胞凋亡。

IF 2.2 4区医学

Toxicology Research Pub Date : 2024-08-14 eCollection Date: 2024-08-01 DOI: 10.1093/toxres/tfae129

Sinan Ince, Hasan Huseyin Demirel, Ezgi Nur Demirkapi, Ismail Kucukkurt, Abdullah Eryavuz, Damla Arslan-Acaroz, Ulas Acaroz, Ali Tureyen

{"title":"Magnolin alleviates cyclophosphamide-induced oxidative stress, inflammation, and apoptosis via Nrf2/HO-1 signaling pathway.","authors":"Sinan Ince, Hasan Huseyin Demirel, Ezgi Nur Demirkapi, Ismail Kucukkurt, Abdullah Eryavuz, Damla Arslan-Acaroz, Ulas Acaroz, Ali Tureyen","doi":"10.1093/toxres/tfae129","DOIUrl":"10.1093/toxres/tfae129","url":null,"abstract":"In the present study, we investigated the protective effect of magnolin (MAG) against oxidative stress induced by cyclophosphamide (CP) and its role in the Nrf2/HO-1 signaling pathway. Rats were administered MAG (1 mg/kg, i.p.) for 14 days and CP (75 mg/kg, i.p.) on the 14th day. CP administration increased tissue damage, as evidenced by elevated levels of transaminases (aspartate and alanine), alkaline phosphatase, and renal parameters (blood urea nitrogen and creatinine). Additionally, 8-hydroxy-2'-deoxyguanosine and malondialdehyde levels were increased, whereas glutathione levels, along with catalase and superoxide dismutase activities, decreased in CP-treated rats. CP also down-regulated the expression of Bcl-2, HO-1, Nrf2, and NQO-1, while up-regulating Bax, Cas-3, TNF-α, Cox-2, iNOS, IL-6, IL-1β, and NFκB in liver and kidney tissues. In addition, CP treatment caused histopathological changes in heart, lung, liver, kidney, brain, and testis tissues. Treatment with MAG improved biochemical and oxidative stress parameters and prevented histopathological changes in CP-treated rats. Moreover, MAG suppressed the expression of inflammatory cytokines and apoptosis markers. In conclusion, MAG effectively prevented CP-induced toxicity by reducing oxidative stress, inflammation, and apoptosis, with its protective efficacy associated with the up-regulation of Nrf2/HO-1 signaling.","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":"13 4","pages":"tfae129"},"PeriodicalIF":2.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0