Contact (Thousand Oaks (Ventura County, Calif.))最新文献

Interorganellar Communication Through Membrane Contact Sites in Toxoplasma Gondii. 弓形虫通过膜接触点进行细胞间通信

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-08-06 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231189064

Diego Huet, Silvia N J Moreno

{"title":"Interorganellar Communication Through Membrane Contact Sites in Toxoplasma Gondii.","authors":"Diego Huet, Silvia N J Moreno","doi":"10.1177/25152564231189064","DOIUrl":"10.1177/25152564231189064","url":null,"abstract":"Apicomplexan parasites are a group of protists that cause disease in humans and include pathogens like Plasmodium spp., the causative agent of malaria, and Toxoplasma gondii, the etiological agent of toxoplasmosis and one of the most ubiquitous human parasites in the world. Membrane contact sites (MCSs) are widespread structures within eukaryotic cells but their characterization in apicomplexan parasites is only in its very beginnings. Basic biological features of the T. gondii parasitic cycle support numerous organellar interactions, including the transfer of Ca2+ and metabolites between different compartments. In T. gondii, Ca2+ signals precede a series of interrelated molecular processes occurring in a coordinated manner that culminate in the stimulation of key steps of the parasite life cycle. Calcium transfer from the endoplasmic reticulum to other organelles via MCSs would explain the precision, speed, and efficiency that is needed during the lytic cycle of T. gondii. In this short review, we discuss the implications of these structures in cellular signaling, with an emphasis on their potential role in Ca2+ signaling.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231189064"},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/fd/10.1177_25152564231189064.PMC10408353.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10305082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial AsmA-Like Proteins: Bridging the Gap in Intermembrane Phospholipid Transport. 细菌 AsmA 样蛋白：弥补膜间磷脂运输的差距

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-07-12 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231185931

Sujeet Kumar, Natividad Ruiz

{"title":"Bacterial AsmA-Like Proteins: Bridging the Gap in Intermembrane Phospholipid Transport.","authors":"Sujeet Kumar, Natividad Ruiz","doi":"10.1177/25152564231185931","DOIUrl":"10.1177/25152564231185931","url":null,"abstract":"In eukaryotic cells, nonvesicular lipid transport between organelles is mediated by lipid-transfer proteins. Recently, a new class of these lipid transporters has been described to facilitate the bulk of inter-organelle lipid transport at contact sites by forming bridge-like structures with a hydrophobic groove through which lipids travel. Because their predicted structure is composed of repeating β-groove (RBG) domains, they have been named the RBG protein superfamily. Early studies on RBG proteins VPS13 and ATG2 recognized the resemblance of their predicted structures to that of the bacterial Lpt system, which transports newly synthesized lipopolysaccharides (LPS) between the inner and the outer membranes (IMs and OMs) of Gram-negative bacteria. In these didermic bacteria, the IMs and OMs are separated by an aqueous periplasmic compartment that is traversed by a bridge-like structure built with β-jelly roll domains from several Lpt proteins that provides a hydrophobic groove for LPS molecules to travel across the periplasm. Despite structural and functional similarities between RBG proteins and the Lpt system, the bacterial AsmA-like protein family has recently emerged as the likely ancestor of RBG proteins and long sought-after transporters that facilitate the transfer of phospholipids from the IM to the OM. Here, we review our current understanding of the structure and function of bacterial AsmA-like proteins, mainly focusing on recent studies that have led to the proposal that AsmA-like proteins mediate the bulk of phospholipid transfer between the IMs and OMs.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231185931"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/86/ae/10.1177_25152564231185931.PMC10345924.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10353105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bridge-Like Lipid Transfer Proteins (BLTPs) in C. elegans: From Genetics to Structures and Functions. 秀丽隐杆线虫桥状脂传递蛋白(BLTPs):从遗传学到结构和功能。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-07-12 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231186489

Taruna Pandey, Jianxiu Zhang, Bingying Wang, Dengke K Ma

{"title":"Bridge-Like Lipid Transfer Proteins (BLTPs) in C. elegans: From Genetics to Structures and Functions.","authors":"Taruna Pandey, Jianxiu Zhang, Bingying Wang, Dengke K Ma","doi":"10.1177/25152564231186489","DOIUrl":"10.1177/25152564231186489","url":null,"abstract":"In eukaryotic cells, lipid transfer can occur at membrane contact sites (MCS) to facilitate the exchange of various lipids between two adjacent cellular organelle membranes. Lipid transfer proteins (LTPs), including shuttle LTP or bridge-like LTP (BLTP), transport lipids at MCS and are critical for diverse cellular processes, including lipid metabolism, membrane trafficking, and cell signaling. BLTPs (BLTP1-5, including the ATG2 and VPS13 family proteins) contain lipid-accommodating hydrophobic repeating β-groove (RBG) domains that allow the bulk transfer of lipids through MCS. Compared with vesicular lipid transfer and shuttle LTP, BLTPs have been only recently identified. Their functions and regulatory mechanisms are currently being unraveled in various model organisms and by diverse approaches. In this review, we summarize the genetics, structural features, and biological functions of BLTP in the genetically tractable model organism C. elegans. We discuss our recent studies and findings on C. elegans LPD-3, a prototypical megaprotein ortholog of BLTP1, with identified lipid transfer functions that are evolutionarily conserved in multicellular organisms and in human cells. We also highlight areas for future research of BLTP using C. elegans and complementary model systems and approaches. Given the emerging links of BLTP to several human diseases, including Parkinson's disease and Alkuraya-Kučinskas syndrome, discovering evolutionarily conserved roles of BLTPs and their mechanisms of regulation and action should contribute to new advances in basic cell biology and potential therapeutic development for related human disorders.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231186489"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f0/3c/10.1177_25152564231186489.PMC10345909.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10353104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IP₃R at ER-Mitochondrial Contact Sites: Beyond the IP₃R-GRP75-VDAC1 Ca²⁺ Funnel. ER-线粒体接触点的 IP3R：超越 IP3R-GRP75-VDAC1 Ca2+ 漏斗。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-06-22 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231181020

Peace Atakpa-Adaji, Adelina Ivanova

{"title":"IP3R at ER-Mitochondrial Contact Sites: Beyond the IP3R-GRP75-VDAC1 Ca2+ Funnel.","authors":"Peace Atakpa-Adaji, Adelina Ivanova","doi":"10.1177/25152564231181020","DOIUrl":"10.1177/25152564231181020","url":null,"abstract":"Membrane contact sites (MCS) circumvent the topological constraints of functional coupling between different membrane-bound organelles by providing a means of communication and exchange of materials. One of the most characterised contact sites in the cell is that between the endoplasmic reticulum and the mitochondrial (ERMCS) whose function is to couple cellular Ca2+ homeostasis and mitochondrial function. Inositol 1,4,5-trisphosphate receptors (IP3Rs) on the ER, glucose-regulated protein 75 (GRP 75) and voltage-dependent anion channel 1 (VDAC1) on the outer mitochondrial membrane are the canonical component of the Ca2+ transfer unit at ERMCS. These are often reported to form a Ca2+ funnel that fuels the mitochondrial low-affinity Ca2+ uptake system. We assess the available evidence on the IP3R subtype selectivity at the ERMCS and consider if IP3Rs have other roles at the ERMCS beyond providing Ca2+. Growing evidence suggests that all three IP3R subtypes can localise and regulate Ca2+ signalling at ERMCS. Furthermore, IP3Rs may be structurally important for assembly of the ERMCS in addition to their role in providing Ca2+ at these sites. Evidence that various binding partners regulate the assembly and Ca2+ transfer at ERMCS populated by IP3R-GRP75-VDAC1, suggesting that cells have evolved mechanisms that stabilise these junctions forming a Ca2+ microdomain that is required to fuel mitochondrial Ca2+ uptake.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231181020"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10352546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Membrane Contact Sites Shape the Phagophore. 膜接触位点如何塑造Phagophore。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-05-09 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231162495

Cristina Capitanio, Anna Bieber, Florian Wilfling

引用次数: 2

VPS13 Forum Proceedings: XK, XK-Related and VPS13 Proteins in Membrane Lipid Dynamics. VPS13 论坛论文集：膜脂动态中的 XK、XK 相关蛋白和 VPS13 蛋白。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-02-23 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231156994

Kevin Peikert, Adrian Danek

引用次数: 0

An Expansion of the Endoplasmic Reticulum that Halts Autophagy is Permissive to Genome Instability. 阻止自噬的内质网扩张会导致基因组不稳定

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-02-22 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231157706

Eliana Lara-Barba, Alba Torán-Vilarrubias, María Moriel-Carretero

{"title":"An Expansion of the Endoplasmic Reticulum that Halts Autophagy is Permissive to Genome Instability.","authors":"Eliana Lara-Barba, Alba Torán-Vilarrubias, María Moriel-Carretero","doi":"10.1177/25152564231157706","DOIUrl":"10.1177/25152564231157706","url":null,"abstract":"The links between autophagy and genome stability, and whether they are important for lifespan and health, are not fully understood. We undertook a study to explore this notion at the molecular level using Saccharomyces cerevisiae. On the one hand, we triggered autophagy using rapamycin, to which we exposed mutants defective in preserving genome integrity, then assessed their viability, their ability to induce autophagy and the link between these two parameters. On the other hand, we searched for molecules derived from plant extracts known to have powerful benefits on human health to try to rescue the negative effects rapamycin had against some of these mutants. We uncover that autophagy execution is lethal for mutants unable to repair DNA double strand breaks, while the extract from Silybum marianum seeds induces an expansion of the endoplasmic reticulum (ER) that blocks autophagy and protects them. Our data uncover a connection between genome integrity and homeostasis of the ER whereby ER stress-like scenarios render cells tolerant to sub-optimal genome integrity conditions.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231157706"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/06/bc/10.1177_25152564231157706.PMC10243512.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10291750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E-Syt1 Regulates Neuronal Activity-Dependent Endoplasmic Reticulum-Plasma Membrane Junctions and Surface Expression of AMPA Receptors. E-Syt1调节神经元活动依赖的内质网-质膜连接和AMPA受体的表面表达。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564231185011

Ranran Mao, Chunfang Tong, Jia-Jia Liu

{"title":"E-Syt1 Regulates Neuronal Activity-Dependent Endoplasmic Reticulum-Plasma Membrane Junctions and Surface Expression of AMPA Receptors.","authors":"Ranran Mao, Chunfang Tong, Jia-Jia Liu","doi":"10.1177/25152564231185011","DOIUrl":"https://doi.org/10.1177/25152564231185011","url":null,"abstract":"Endoplasmic reticulum (ER)-plasma membrane (PM) contact sites/junctions play important roles in cell physiology including signal transduction, ion and lipid transfer, and membrane dynamics. However, little is known about the dynamic regulation and functional roles of ER-PM junctions in neurons. Using a split green fluorescent protein-based membrane contact probe, we find that the density of ER-PM contact sites changes dynamically in the dendrites of hippocampal neurons undergoing long-term synaptic potentiation (LTP). We show that the Ca2±-sensing membrane tethering protein Extended Synaptotagmin 1 (E-Syt1) mediates the formation of ER-PM contact sites during LTP. We also show that E-Syt1 is required for neuronal activity-dependent surface expression of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid-type glutamate receptors. These findings implicate ER-PM junctions in the regulation of neurotransmitter receptor trafficking and synaptic plasticity.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231185011"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/81/db/10.1177_25152564231185011.PMC10359807.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10665745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complementation Assay Using Fusion of Split-GFP and TurboID (CsFiND) Enables Simultaneous Visualization and Proximity Labeling of Organelle Contact Sites in Yeast. 利用Split-GFP和TurboID (CsFiND)的融合进行互补分析，可以同时可视化和接近标记酵母细胞器接触位点。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564231153621

Shintaro Fujimoto, Shinya Tashiro, Yasushi Tamura

引用次数: 0

Evolutionary History of Oxysterol-Binding Proteins Reveals Complex History of Duplication and Loss in Animals and Fungi. 动物和真菌中氧化甾醇结合蛋白的进化史揭示了复制和丢失的复杂历史。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564221150428

Rohan P Singh, Yu-Ping Poh, Savar D Sinha, Jeremy G Wideman

{"title":"Evolutionary History of Oxysterol-Binding Proteins Reveals Complex History of Duplication and Loss in Animals and Fungi.","authors":"Rohan P Singh, Yu-Ping Poh, Savar D Sinha, Jeremy G Wideman","doi":"10.1177/25152564221150428","DOIUrl":"https://doi.org/10.1177/25152564221150428","url":null,"abstract":"Cells maintain the specific lipid composition of distinct organelles by vesicular transport as well as non-vesicular lipid trafficking via lipid transport proteins. Oxysterol-binding proteins (OSBPs) are a family of lipid transport proteins that transfer lipids at various membrane contact sites (MCSs). OSBPs have been extensively investigated in human and yeast cells where 12 have been identified in Homo sapiens and 7 in Saccharomyces cerevisiae. The evolutionary relationship between these well-characterized OSBPs is still unclear. By reconstructing phylogenies of eukaryote OSBPs, we show that the ancestral Saccharomycotina had four OSBPs, the ancestral fungus had five OSBPs, and the ancestral animal had six OSBPs, whereas the shared ancestor of animals and fungi as well as the ancestral eukaryote had only three OSBPs. Our analyses identified three undescribed ancient OSBP orthologues, one fungal OSBP (Osh8) lost in the lineage leading to yeast, one animal OSBP (ORP12) lost in the lineage leading to vertebrates, and one eukaryotic OSBP (OshEu) lost in both the animal and fungal lineages.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564221150428"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0