Contact (Thousand Oaks (Ventura County, Calif.))最新文献_第2页

Extending the Contacts Breaks the Flow. 扩展联系人打破流程。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564221125045

Amado Carreras-Sureda, Christopher Henry, Nicolas Demaurex

引用次数: 0

The Organization and Function of the Phagophore-ER Membrane Contact Sites. 吞噬体-内质网膜接触位点的组织和功能。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564231183898

Prado Vargas Duarte, Fulvio Reggiori

{"title":"The Organization and Function of the Phagophore-ER Membrane Contact Sites.","authors":"Prado Vargas Duarte, Fulvio Reggiori","doi":"10.1177/25152564231183898","DOIUrl":"https://doi.org/10.1177/25152564231183898","url":null,"abstract":"Macroautophagy is characterized by the de novo formation of double-membrane vesicles termed autophagosomes. The precursor structure of autophagosomes is a membrane cistern called phagophore, which elongates through a massive acquisition of lipids until closure. The phagophore establishes membrane-contact sites (MCSs) with the endoplasmic reticulum (ER), where conserved ATG proteins belonging to the ATG9 lipid scramblase, ATG2 lipid transfer and Atg18/WIPI4 β-propeller families concentrate. Several recent in vivo and in vitro studies have uncovered the relevance of these proteins and MCSs in the lipid supply required for autophagosome formation. Although important conceptual advances have been reached, the functional interrelationship between ATG9, ATG2 and Atg18/WIPI4 proteins at the phagophore-ER MCSs and their role in the phagophore expansion are not completely understood. In this review, we describe the current knowledge about the structure, interactions, localizations, and molecular functions of these proteins, with a particular emphasis on the yeast Saccharomyces cerevisiae and mammalian systems.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231183898"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/f5/10.1177_25152564231183898.PMC10350784.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10648390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "VPS13A and VPS13C Influence Lipid Droplet Abundance". “VPS13A和VPS13C影响脂滴丰度”的勘误表。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564231175732

引用次数: 0

Stranger Twins: A Tale of Resemblance and Contrast Between VAP Proteins. 陌生的双胞胎:VAP蛋白之间的相似和对比。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-01-01 DOI: 10.1177/25152564231183897

Mélody Subra, Zoé Grimanelli, Romain Gautier, Bruno Mesmin

引用次数: 0

VPS13A and VPS13C Influence Lipid Droplet Abundance. VPS13A 和 VPS13C 影响脂滴丰度

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-09-13 eCollection Date: 2022-01-01 DOI: 10.1177/25152564221125613

Shuliang Chen, Melissa A Roberts, Chun-Yuan Chen, Sebastian Markmiller, Hong-Guang Wei, Gene W Yeo, James G Granneman, James A Olzmann, Susan Ferro-Novick

{"title":"VPS13A and VPS13C Influence Lipid Droplet Abundance.","authors":"Shuliang Chen, Melissa A Roberts, Chun-Yuan Chen, Sebastian Markmiller, Hong-Guang Wei, Gene W Yeo, James G Granneman, James A Olzmann, Susan Ferro-Novick","doi":"10.1177/25152564221125613","DOIUrl":"10.1177/25152564221125613","url":null,"abstract":"Lipid transfer proteins mediate the exchange of lipids between closely apposed membranes at organelle contact sites and play key roles in lipid metabolism, membrane homeostasis, and cellular signaling. A recently discovered novel family of lipid transfer proteins, which includes the VPS13 proteins (VPS13A-D), adopt a rod-like bridge conformation with an extended hydrophobic groove that enables the bulk transfer of membrane lipids for membrane growth. Loss of function mutations in VPS13A and VPS13C cause chorea acanthocytosis and Parkinson's disease, respectively. VPS13A and VPS13C localize to multiple organelle contact sites, including endoplasmic reticulum (ER) - lipid droplet (LD) contact sites, but the functional roles of these proteins in LD regulation remains mostly unexplored. Here we employ CRISPR-Cas9 genome editing to generate VPS13A and VPS13C knockout cell lines in U-2 OS cells via deletion of exon 2 and introduction of an early frameshift. Analysis of LD content in these cell lines revealed that loss of either VPS13A or VPS13C results in reduced LD abundance under oleate-stimulated conditions. These data implicate two lipid transfer proteins, VPS13A and VPS13C, in LD regulation.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"5 ","pages":"25152564221125613"},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9703031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Csf1: A Putative Lipid Transport Protein Required for Homeoviscous Adaptation of the Lipidome. Csf1：脂质体同黏适应所需的假定脂质运输蛋白

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-06-28 eCollection Date: 2022-01-01 DOI: 10.1177/25152564221101974

Arun T John Peter, Ngaam J Cheung, Benoît Kornmann

{"title":"Csf1: A Putative Lipid Transport Protein Required for Homeoviscous Adaptation of the Lipidome.","authors":"Arun T John Peter, Ngaam J Cheung, Benoît Kornmann","doi":"10.1177/25152564221101974","DOIUrl":"10.1177/25152564221101974","url":null,"abstract":"The non-vesicular transport of lipids between organelles mediated by lipid transport proteins (LTPs) is a key determinant of organelle biogenesis and function. Despite performing a vital function in organelle homeostasis, none of the LTP-encoding genes identified so far are truly essential, even in the simple genome of yeast, suggesting widespread redundancy. In line with this fact, it has been found that a number of LTPs have overlapping functions, making it challenging to assign unique roles for an individual LTP in lipid distribution. In our genetic screens under stringent conditions in which the distinct function of an LTP might become essential, we stumbled upon Csf1, a highly conserved protein with a Chorein-N motif found in other lipid transporters and unraveled a new function for Csf1 in lipid remodeling and homeoviscous adaptation of the lipidome. Here, we further speculate on the potential mechanisms of how the putative function of Csf1 in lipid transport could be intimately connected to its role in lipid remodeling across organelles.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"5 ","pages":"25152564221101974"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10298549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moving Lipids, by the Numbers. 移动的脂质，通过数字。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-01-01 DOI: 10.1177/25152564221103080

Pascal F Egea

引用次数: 1

The PH-like Domain of VPS13 Proteins - a Determinant of Localization to the Golgi Apparatus or to the Plasma Membrane. VPS13蛋白的ph样结构域-定位于高尔基体或质膜的决定因素。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-01-01 DOI: 10.1177/25152564221106024

Joanna Kaminska

引用次数: 0

ER membrane contact sites: key platforms for biogenesis of RNA-containing extracellular vesicles. ER膜接触位点：含有RNA的细胞外小泡生物发生的关键平台。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-01-01 Epub Date: 2022-09-06 DOI: 10.1177/25152564221121444

Bahnisikha Barman, T Renee Dawson, Alissa M Weaver

{"title":"ER membrane contact sites: key platforms for biogenesis of RNA-containing extracellular vesicles.","authors":"Bahnisikha Barman, T Renee Dawson, Alissa M Weaver","doi":"10.1177/25152564221121444","DOIUrl":"10.1177/25152564221121444","url":null,"abstract":"The mechanisms by which cytoplasmic cargoes such as RNAs are incorporated into extracellular vesicles (EVs) are poorly understood. In a recent article published in Developmental Cell, we describe a novel function of endoplasmic reticulum membrane contact sites (ER MCS) in regulating biogenesis of RNA-containing EVs (Barman et al., 2022). We identified the ER MCS tether protein VAP-A and the ceramide transporter CERT as key drivers of this process. VAP-A depletion and overexpression produced corresponding changes in the overall number and RNA content of secreted EVs. Further sub-fractionation of small EVs from VAP-A depleted cells revealed a distinct loss in a specific subset of dense, RNA-loaded small EVs that are critical for the transfer of miR-100 to recipient cells. Cell imaging data confirmed the loss of RNA and RNA binding proteins (RBPs) in VAP-A-knockdown multivesicular bodies. Lipid analysis of VAP-A-knockdown EVs revealed decreases in ceramides, which are known to affect EV biogenesis. Depletion of the ceramide transfer protein CERT, which interacts with its binding partner VAP-A at ER MCS, leads to similar defects in EV number and RNA content as VAP-A-knockdown. These data suggest a model for ER MCS as platforms for biogenesis of a key EV population via ceramide transfer and RNA loading.","PeriodicalId":10556,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"5 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/7f/10.1177_25152564221121444.PMC10065479.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9760084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sorting (Nexin-13) out Novel Insights into Endolysosomal Cholesterol Export. 分类(Nexin-13)对内溶酶体胆固醇输出的新见解。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2022-01-01 DOI: 10.1177/25152564221114513

Albert Lu

引用次数: 0