Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie最新文献_第3页

Human erythroid spectrin alpha subunit and its SH3 domain are sensitive to acidic Plasmodium falciparum proteolytic activity. 人红细胞谱蛋白α亚基及其SH3结构域对酸性恶性疟原虫蛋白水解活性敏感。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

S L Le Bonniec, C Fournier, C Deregnaucourt, P Grellier, D Dhermy, M C Lecomte, J Schrevel

引用次数: 0

[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process]. [转化生长因子β 1 (tgf - β 1)和血管内皮生长因子(VEGF)在体外血管生成过程中的作用]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

B Vailhé, L Tranqui

{"title":"[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process].","authors":"B Vailhé, L Tranqui","doi":"","DOIUrl":"","url":null,"abstract":"We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, on the in vitro angiogenesis process. Endothelial cells were plated on fibrin matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours later, with 10% human serum, the capillary-like network index (the percentage of the culture area covered by the capillary network) was about 50%. Under these culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like network index augmented and was nearing 75%. When the index is high, using confocal microscopy, we show that hollow capillaries are formed. Moreover, the addition of VEGF increased the kinetics of the capillary-like network formation. With 2% human serum, 48 h after seeding the capillary-like network index was about 75%. In this case, the addition of TGF-beta 1 decreased the network index, whereas the addition of VEGF increased the kinetics of its formation. These in vitro angiogenesis experiments show that the serological factors underline the 2 antagonist effects of TGF-beta 1, but have no detectable effects on the activator effects on the VEGF.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 11","pages":"1003-10"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19992735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The applicability of hematoxylin-eosin staining plus fluorescence or confocal laser scanning microscopy to the study of elastic fibers in cartilages. 苏木精-伊红染色加荧光或共聚焦激光扫描显微镜在软骨弹性纤维研究中的适用性。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

H F de Carvalho, S R Taboga

引用次数: 0

[Recovery of ovarian function after radiation-induced menopause. Does follicle-stimulating hormone (FSH) have a definitive prognostic value?]. 放射绝经后卵巢功能的恢复。促卵泡激素(FSH)是否具有明确的预后价值?

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

H Letur-Könirsch, F Guis

{"title":"[Recovery of ovarian function after radiation-induced menopause. Does follicle-stimulating hormone (FSH) have a definitive prognostic value?].","authors":"H Letur-Könirsch, F Guis","doi":"","DOIUrl":"","url":null,"abstract":"Menopause, conventionally defined as the permanent cessation of menstruation as a result of loss of ovarian follicular activity, is biologically expressed by the collapse of plasma estradiol levels and increased plasma levels of the gonadotrophins FSH (follicle stimulating hormone) and LH (luteinizing hormone). At present, estimation of the ovarian follicle reserve is based on endocrine capacity tests of the ovaries, with increased FSH representing the first sign of exocrine ovarian failure. We report the case of one of our amenorrhoeic patients after chemotherapy, total body radiation and allogenic bone marrow transplantation for acute immunoblastic leukaemia. This patient was included in an in vitro fertilization with oocyte donation (IVF-OD) programme for iatrogenic premature ovarian failure with increased FSH levels. Instead of high levels of gonadotrophins, this young woman recovered spontaneous follicular development, benefited from standard IVF with her own oocytes and brought a twin pregnancy to term. This observation shows that a high FSH level is not a definitive prediction of ovarian exocrine capacity. In young women of child-bearing age such as these wanting a child and showing signs of endogenous estrogen impregnation, evaluation of the existence and quality of follicular development is an important factor.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 10","pages":"907-12"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19939556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Search for the candidate genes in dominant hereditary spherocytosis using linkage analysis. 利用连锁分析寻找显性遗传性球形红细胞增多症的候选基因。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

M Garbarz, D Bibas, T Cynober, C Galand, O Bournier, I Devaux, G Tchernia, D Dhermy

{"title":"Search for the candidate genes in dominant hereditary spherocytosis using linkage analysis.","authors":"M Garbarz, D Bibas, T Cynober, C Galand, O Bournier, I Devaux, G Tchernia, D Dhermy","doi":"","DOIUrl":"","url":null,"abstract":"Hereditary spherocytosis (HS) is an inherited hemolytic anemia characterized by the presence of dense spherocytic red cells. In HS patients, red cell membrane protein gel electrophoresis has identified different subsets of abnormalities: isolated spectrin deficiency, combined spectrin and ankyrin deficiency, band 3 deficiency. To direct the search for the molecular defect in 9 families with dominant HS, we developed microsatellite markers specific for the membrane protein encoding genes possibly involved in HS (alpha- and beta-spectrin, ankyrin and band 3 genes) and genotyped each family. In 5 families with isolated spectrin deficiency, the beta-spectrin gene was designated as candidate. In one family with combined spectrin/ankyrin deficiency, only the ankyrin gene was not excluded, whereas in the 3 HS families with band 3 deficiency, only the band 3 gene was not excluded. This work allowed development of a reliable methodology to search for candidate genes in HS and showed the frequent involvement of the beta-spectrin gene in HS with isolated spectrin deficiency.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 10","pages":"913-9"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19939557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Rapid identification of chromosomes by in situ hybridization of labelled oligonucleotides and comparison with the PRINS method]. [通过标记寡核苷酸原位杂交快速鉴定染色体并与PRINS方法比较]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

P Coullin, A Valent, I Barbounaki, J J Candelier, F Pellestor, A Bernheim

{"title":"[Rapid identification of chromosomes by in situ hybridization of labelled oligonucleotides and comparison with the PRINS method].","authors":"P Coullin, A Valent, I Barbounaki, J J Candelier, F Pellestor, A Bernheim","doi":"","DOIUrl":"","url":null,"abstract":"We propose a simple, fast and inexpensive method of identification of human centromeres on metaphasic chromosomes and interphasic nuclei. This is based on in situ hybridization of labelled oligonucleotides. The efficiency of the methodology was demonstrated on cytogenetic preparations from human heteroploid and human x hamster hybrid cell lines and also on frozen tissue sections using an oligonucleotide specific for the alpha-satellite DNA of chromosome 1. Three versions of this oligonucleotide respectively labelled with 1, 4 and 10 fluorescein molecules were synthesized. The signal intensity provided by the oligonucleotide coupled with 4 fluoresceins allowed unambiguously the detection of the chromosome and the establishment of its ploidy using a classical cytogenetic microscope without the need for an amplification procedure. The use of different fluorochromes and possibly combination with an unlabelled elongation in 3' of the oligonucleotides which stabilize its hybridization, lead to a simple multicolour method. Preliminary quantification of the signals obtained by in situ hybridization of labelled oligonucleotides and comparison with those obtained by primed in situ labelling (PRINS) using the same nucleotides as primers, suggest that the elongation generated by PRINS may be very short compared with a PCR in solution. This limited efficiency of the in situ elongation may reflect the present difficulties of PRINS and DISC PCR (direct in situ single copy polymerase chain reaction) with primers specific for non-repetitive sequencies.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 10","pages":"901-6"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19939555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The APC gene product and colorectal carcinogenesis. APC基因产物与结直肠癌的发生。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

C Bonneton, L Larue, J P Thiéry

引用次数: 0

Apoptotic death in cortical neurons of mice subjected to focal ischemia. 局灶性缺血小鼠皮质神经元凋亡的研究。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

C Guégan, H Boutin, C Boudry, E T MacKenzie, B Sola

引用次数: 0

[Karyologic study of gill neoplasia in Macoma balthica (Mollusca, Bivalvia)]. [balthica Macoma(软体动物，Bivalvia)鳃肿瘤的核生物学研究]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

C Thiriot-Quiévreux, M Wolowicz

{"title":"[Karyologic study of gill neoplasia in Macoma balthica (Mollusca, Bivalvia)].","authors":"C Thiriot-Quiévreux, M Wolowicz","doi":"","DOIUrl":"","url":null,"abstract":"This paper reports the first karyological study of a gill neoplasia in the bivalve Macoma balthica from the Bay of Gdansk (Poland). Chromosomes were studied with an air-drying technique from gill tissue. Out of 47 specimens studied, 34 showed normal cells and a variable number of mitotic metaphases with a normal diploid chromosome number of 2n = 38, 6 had hypertrophied nuclei and a high number of mitoses with 70 to 98 chromosomes, and 7 specimens showed intermediate features. The karyotype of normal metaphases included 11 metacentric, 2 submetacentric and 6 subtelocentric chromosome pairs. The karyotype of abnormal metaphases, i.e. with a high number of chromosomes, revealed chromosomal aberrations inferring neoplastic disorders such as: different number of metacentric, submetacentric, subtelocentric and telocentric chromosome pairs than in the normal karyotype, increase of chromosome pairs especially in the small and medium-sized chromosomes, irregular monosomy and occurrence of microchromosomes. According to neoplasias recorded in bivalves species, the prevalence observed in this neoplasia is relatively high. As Macoma balthica inhabits the polluted Bay of Gdansk, the effects of environmental parameters should be elucidated.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 10","pages":"887-92"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19939554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation but not ras-transfection increases the expression of galectin-3 in human HOS cells. 转化而非ras转染可增加人HOS细胞中半乳糖凝集素-3的表达。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-10-01

E Hébert, A C Roche, M Nachtigal, M Monsigny

引用次数: 0