Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie最新文献_第2页

[Follicular maturation, dizygotic twin conceptions and maternal age]. [卵泡成熟，异卵双胞胎受孕和母亲年龄]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

P Lazar

引用次数: 0

[New hypothesis on the replication of centrioles and basal bodies]. [关于中心粒和基体复制的新假设]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

J P Mignot

引用次数: 0

Inhibition of rat cerebral mitochondrial respiration by cyclosporins A, D, and G and restoration with trimetazidine. 环孢素A、D和G对大鼠脑线粒体呼吸的抑制作用及曲美他嗪的恢复作用。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

R Zini, N Simon, C Morin, P d'Athis, J P Tillement

{"title":"Inhibition of rat cerebral mitochondrial respiration by cyclosporins A, D, and G and restoration with trimetazidine.","authors":"R Zini, N Simon, C Morin, P d'Athis, J P Tillement","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this work was to investigate possible inhibitory effects of Ca+ and different cyclosporins (Cs) on the rat brain mitochondrial respiratory control ratio (RCR) and whether or not these effects could be antagonized by trimetazidine (TMZ). The RCR was evaluated as the state 3/state 4 ratio of oxidative phosphorylation. CsA, D, and G inhibited about 10% of RCR in a concentration-dependent manner with EC50 of 57, 19 and 7 nM, respectively, whereas CsH did not modify RCR. TMZ was able to fully antagonize this inhibitory effect in a concentration-dependent manner with EC50 of 5,200, 180, and 1 nM, respectively. The Ca2+ added to the mitochondrial preparation decreased RCR in a concentration-dependent manner with a maximal effect of 46% obtained with 100 microM Ca2+. In the presence of TMZ (100 microM), the inhibitory effect of Ca2+ was partly reversed (9%). TMZ alone showed no inhibitory or stimulant effect on RCR. These results show that restoration of RCR by TMZ is due to a Ca(2+)-dependent mechanism, promoting Ca2+ efflux from the mitochondrial matrix. However, Ca2+ efflux is only partial in case of Ca2+ overload. These data suggest that TMZ may restore ATP synthesis in circumstances where neither Ca2+ overload, nor a prooxidant have generated a RCR decrease.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 12","pages":"1087-92"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20042038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory interactions controlling organizer activity in fish. 控制鱼类组织体活性的抑制相互作用。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

N Peyriéras, Y Lu, A Renucci, V Lemarchandel, F Rosa

引用次数: 0

Classifying individuals among infra-specific taxa using microsatellite data and neural networks. 利用微卫星数据和神经网络对亚种分类群进行个体分类。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

J M Cornuet, S Aulagnier, S Lek, S Franck, M Solignac

{"title":"Classifying individuals among infra-specific taxa using microsatellite data and neural networks.","authors":"J M Cornuet, S Aulagnier, S Lek, S Franck, M Solignac","doi":"","DOIUrl":"","url":null,"abstract":"The method of neural networks was tested for its ability to assign individuals on the basis of their multilocus genotypes, using a data collection of 430 honeybees and 8 microsatellite loci. This data set includes various taxonomical levels (populations within the same subspecies, various subspecies belonging to the same evolutionary lineage, and the 3 lineages of the species). Qualitative genotypic data have been submitted to 2 types of transformation (simple coding and coding plus factorial correspondence analysis), and they have been partitioned in 2 sets, a training set of 300 individuals and a testing set of 103 individuals. Two procedures (\"leave one out\" and \"hold out\") were applied to evaluate the quality of prediction. Compared to discriminant analysis, neural networks performed better in terms of correctly classified individuals at any taxonomical level. For instance, with the simple coding and the hold out procedure, the proportions of correctly assigned individuals from the testing set were 66.2%, 82.3% and 100% at the populations, subspecies and lineage level, respectively. The potential use of neural networks in populations genetics is discussed.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 12","pages":"1167-77"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20042315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The activities of enzymes associated with the intermediary and energy metabolism in hypogean and epigean crustaceans. 下生和上生甲壳类中与中间代谢和能量代谢有关的酶的活性。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

F Hervant

{"title":"The activities of enzymes associated with the intermediary and energy metabolism in hypogean and epigean crustaceans.","authors":"F Hervant","doi":"","DOIUrl":"","url":null,"abstract":"The activities of 18 enzymes involved in the intermediary and energy metabolism were measured in certain widely-spread peracarid crustaceans: 3 hypogean (Niphargus virei, Niphargus rhenorhodanensis and Stenasellus virei) and 2 epigean (Gammarus fossarum and Asellus aquaticus) ones. The activities of numerous enzymes were correlated with the known metabolic rates of the 5 species. Such rates are reduced in hypogean organisms: levels of enzymatic activity in subterranean species were 1.2 to 8.6 times lower than in epigean species for the main key regulatory enzymes involved in the Krebs cycle and glycolysis (phosphofructokinase, pyruvate kinase, hexokinase and citrate synthetase). The relative activities of phosphofructokinase, glycogen phosphorylase and hexokinase clearly indicated that glycogen was the main fuel oxidized in both epigean and hypogean organisms. A higher glycogen phosphorylase/hexokinase ratio in hypogean than in epigean crustaceans showed that subterranean species had a greater ability to function anaerobically. The presence of high activities of glutamate-pyruvate transaminase and lactate dehydrogenase in all species (and of malate dehydrogenase and fumarase in hypogean species) was indicative of a coupled fermentation of glycogen and glutamate during anaerobiosis, with lactate and alanine as end-products (as well as succinate in hypogean species). A low fructose-1,6-bisphosphatase/phosphofructokinase ratio, associated with a low level of phosphoenolpyruvate carboxykinase activity, indicated that the glycolytic pathway was active and that gluconeogenic ability was limited in epigean crustaceans. In contrast, in hypogean species, association of a higher ratio and a high level of phosphoenolpyruvate carboxykinase activity suggested a low glycolytic activity and a high gluconeogenic ability.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 12","pages":"1071-7"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20042036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Contribution to the study of the origin of cellulase activities of the gut in earthworm Eisenia fetida andrei]. [对蚯蚓肠道纤维素酶活性起源研究的贡献]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-12-01

M Vinceslas-Akpa, M Loquet

引用次数: 0

A lobster cysteine protease with immunoreactivity and activities of calcitonin and CGRP. 一种具有免疫反应性和降钙素和CGRP活性的龙虾半胱氨酸蛋白酶。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

Y Arlot-Bonnemains, G Milhaud, M Fouchereau-Péron

{"title":"A lobster cysteine protease with immunoreactivity and activities of calcitonin and CGRP.","authors":"Y Arlot-Bonnemains, G Milhaud, M Fouchereau-Péron","doi":"","DOIUrl":"","url":null,"abstract":"The high concentrations of molecules immunologically related to salmon calcitonin (CT) and/or to human calcitonin gene-related peptide (CGRP) in the oesophagus of the norway lobster Nephrops norvegicus have been examined. In the present study. We report the purification of these molecules by means of a specific radioimmunoassay for calcitonin and calcitonin gene related peptide. The immunoreactive molecules were tested for their functional similarities with CT and CGRP. This was investigated by measuring their ability to interact with CGRP and CT radioreceptor assays and to stimulate the adenylate cyclase activity in rat liver and kidney membranes, respectively. In addition, the purified product was injected in young rats in order to check for a CT-like biological activity of these molecules. The combination of these tests led us to purify a molecular form of 33 kDa. N-terminal sequence analysis of this protein revealed a considerable homology with the lobster cysteine proteases and the human cathepsin L. Control experiments performed with the highly purified American lobster cysteine protease I showed that crustacean cysteine proteases given in vivo to rats induce a fall in the plasma calcium and phosphate levels. This study therefore adds further documentation for a common ancestral origin of CT, CGRP and the much large cysteine proteases from invertebrates.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 11","pages":"975-82"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19992846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial purification of the pig kidney cystic fibrosis transmembrane regulator protein. 猪肾囊性纤维化跨膜调节蛋白的部分纯化。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

M Benharouga, J Fritsch, M Goossens, A Edelman

引用次数: 0

[Cloning, expression and characterization of HIV-1 gp60 partially or completely deleted in the V3 loop]. [HIV-1 gp60在V3环部分或完全缺失的克隆、表达和表征]。

Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1996-11-01

C Lavallée, L Thibodeau

{"title":"[Cloning, expression and characterization of HIV-1 gp60 partially or completely deleted in the V3 loop].","authors":"C Lavallée, L Thibodeau","doi":"","DOIUrl":"","url":null,"abstract":"In order to better understand the role of the immunodominant V3 loop in the type-specific immune response and also to determine if this sequence has a role in AIDS pathogenesis, notably in the induction of apoptosis in CD4+ cells, we have introduced 2 modifications in the env gene from pNL4-3: a partial deletion in the V3 loop, keeping only the conserved tip of the loop GPGRAF consensus sequence (env delta V3-GPGRAF) and, secondly, a complete deletion of V3 sequence plus 43 nucleotides in C3 (env delta V3+). These constructions as well as the non-modified env gene, were cloned and expressed in a baculovirus system. Western blot analysis has shown that both modified env gene products reacted with a reference anti-HIV-1 serum to the same extent as the non-modified gp 160. However, in contrast to the non-modified env-protein and to env delta V3-GPGRAF, the env delta V3+ protein failed to bind to CD4 molecule, although V3 is not directly involved in receptor binding. These modified and non-modified recombinant proteins will be very useful to determine the potential of the partially or totally V3-deleted gp 160 to induce broadly reactive neutralizing antibodies and also to determine if V3 has a role in certain aspects of HIV-induced pathogenesis, notably apoptosis.","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 11","pages":"983-90"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19992734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0