[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process].
{"title":"[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process].","authors":"B Vailhé, L Tranqui","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, on the in vitro angiogenesis process. Endothelial cells were plated on fibrin matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours later, with 10% human serum, the capillary-like network index (the percentage of the culture area covered by the capillary network) was about 50%. Under these culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like network index augmented and was nearing 75%. When the index is high, using confocal microscopy, we show that hollow capillaries are formed. Moreover, the addition of VEGF increased the kinetics of the capillary-like network formation. With 2% human serum, 48 h after seeding the capillary-like network index was about 75%. In this case, the addition of TGF-beta 1 decreased the network index, whereas the addition of VEGF increased the kinetics of its formation. These in vitro angiogenesis experiments show that the serological factors underline the 2 antagonist effects of TGF-beta 1, but have no detectable effects on the activator effects on the VEGF.</p>","PeriodicalId":10555,"journal":{"name":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","volume":"319 11","pages":"1003-10"},"PeriodicalIF":0.0000,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, on the in vitro angiogenesis process. Endothelial cells were plated on fibrin matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours later, with 10% human serum, the capillary-like network index (the percentage of the culture area covered by the capillary network) was about 50%. Under these culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like network index augmented and was nearing 75%. When the index is high, using confocal microscopy, we show that hollow capillaries are formed. Moreover, the addition of VEGF increased the kinetics of the capillary-like network formation. With 2% human serum, 48 h after seeding the capillary-like network index was about 75%. In this case, the addition of TGF-beta 1 decreased the network index, whereas the addition of VEGF increased the kinetics of its formation. These in vitro angiogenesis experiments show that the serological factors underline the 2 antagonist effects of TGF-beta 1, but have no detectable effects on the activator effects on the VEGF.