[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process].

B Vailhé, L Tranqui
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引用次数: 0

Abstract

We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, on the in vitro angiogenesis process. Endothelial cells were plated on fibrin matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours later, with 10% human serum, the capillary-like network index (the percentage of the culture area covered by the capillary network) was about 50%. Under these culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like network index augmented and was nearing 75%. When the index is high, using confocal microscopy, we show that hollow capillaries are formed. Moreover, the addition of VEGF increased the kinetics of the capillary-like network formation. With 2% human serum, 48 h after seeding the capillary-like network index was about 75%. In this case, the addition of TGF-beta 1 decreased the network index, whereas the addition of VEGF increased the kinetics of its formation. These in vitro angiogenesis experiments show that the serological factors underline the 2 antagonist effects of TGF-beta 1, but have no detectable effects on the activator effects on the VEGF.

[转化生长因子β 1 (tgf - β 1)和血管内皮生长因子(VEGF)在体外血管生成过程中的作用]。
我们研究了2种外源性细胞因子TGF β 1和VEGF在体外血管生成过程中的作用。在199培养基中,内皮细胞分别在含2%或10%人血清的纤维蛋白基质上镀。48小时后,添加10%的人血清,毛细血管样网络指数(毛细血管网络覆盖的培养面积百分比)约为50%。在这些培养条件下,当添加tgf - β 1或VEGF时,毛细血管样网络指数增加,接近75%。当指数高时,使用共聚焦显微镜,我们发现空心毛细血管形成。此外,VEGF的加入增加了毛细血管样网络形成的动力学。在2%人血清的条件下,48 h后毛细血管样网络指数约为75%。在这种情况下,tgf - β 1的加入降低了网络指数,而VEGF的加入增加了其形成的动力学。这些体外血管生成实验表明,血清学因素强调tgf - β 1的2种拮抗剂作用,但对VEGF的激活剂作用没有可检测到的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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