Clinical Microbiology and Infection最新文献

{"title":"Revolution in publication: if not now, when?","authors":"Jean-Philippe Lanoix, Asma Nasim, Elda Righi, Dafna Yahav","doi":"10.1016/j.cmi.2025.07.005","DOIUrl":"10.1016/j.cmi.2025.07.005","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to interpret MICs of amphotericin B, echinocandins and flucytosine against Candida auris (Candidozyma auris) according to the newly established European Committee for Antimicrobial Susceptibility Testing (EUCAST) breakpoints.","authors":"Maiken Cavling Arendrup, Jesus Guinea, Sevtap Arikan-Akdagli, Eelco F J Meijer, Jacques F Meis, Jochem B Buil, Eric Dannaoui, Christian G Giske, Pavlina Lyskova, Joseph Meletiadis","doi":"10.1016/j.cmi.2025.07.002","DOIUrl":"10.1016/j.cmi.2025.07.002","url":null,"abstract":"<p><strong>Background: </strong>Candida auris (Candidozyma auris) has emerged as an important pathogen across all continents, with clonal outbreaks and hospital transmissions. Most isolates are fluconazole resistant, and variable resistance rates are reported for amphotericin B and echinocandins.</p><p><strong>Objectives: </strong>This study aimed to present an overview of the newly established epidemiological cut-off values (ECOFFs) and antifungal breakpoints against C auris and the supporting evidence.</p><p><strong>Sources: </strong>This document is based on the recently updated European Committee for Antimicrobial Susceptibility Testing (EUCAST) rationale documents, clinical breakpoint, and ECOFF documents.</p><p><strong>Content: </strong>An alternative approach was adopted for ECOFF setting of C. auris to avoid MIC distributions dominated by isogenic outbreak strains. A carefully selected strain collection of 30 isolates from 11 countries, representing five clades and 21 unique genotypes, was shared among five individual laboratories. MICs were determined with the EUCAST E.Def 7.4 methodology, providing five non-clonal datasets well above the required ≥100 total MICs per drug. Available PK-PD and clinical data were reviewed.</p><p><strong>Implications: </strong>The following ECOFFs and breakpoints were established for C auris: amphotericin B: ECOFF: 2 mg/L, S: ≤0.001 mg/L, R: >2 mg/L; implying that the entire wild-type distribution is susceptible, increased exposure (I) (increased dose: 5 mg/kg liposomal amphotericin B daily); anidulafungin and micafungin: ECOFFs: 0.25 mg/L, S: ≤0.25; R: >0.25; rezafungin: ECOFF: 0.125 mg/L; and flucytosine: ECOFF: 0.5 mg/L. Importantly, notable MIC variations have been reported for C auris and some agents across commercial tests. Consequently, important detailed guidance is provided on how to validate your MIC test in-house before adopting the EUCAST breakpoints for MIC interpretation.</p>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected natural cefiderocol resistance in Stenotrophomonas maltophilia associated to the genogroup 4 genetic background","authors":"Céline Sakr ,&nbsp;Maxime Danjean ,&nbsp;Florence Cizeau ,&nbsp;David Ducellier ,&nbsp;Melissa N. Debi ,&nbsp;Guilhem Royer ,&nbsp;Laurent Poirel ,&nbsp;Jean-Winoc Decousser","doi":"10.1016/j.cmi.2025.07.001","DOIUrl":"10.1016/j.cmi.2025.07.001","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Stenotrophomonas maltophilia</em> (Sm) is responsible for infections in immunocompromised and hospitalized patients. Its genomic diversity has led to the description of a large complex including numerous genogroups. Regarding acquired resistances, cefiderocol (CeFiDeroCol, CFDC) is a promising therapeutic option. We aimed to evaluate the CFDC susceptibility of a large panel of Sm strains and explored the genetic support and background of resistance.</div></div><div><h3>Methods</h3><div>We prospectively collected 154 non-duplicated clinical and environmental strains from five university hospitals between January 2023 and April 2024. All the strains were whole-genome sequenced and their genetic background studied using multi-locus sequence typing , core genome multi-locus sequence typing, and genogroup determination. CFDC susceptibility was tested using a two-step approach with a marketed product (screening step) and the broth microdilution method (confirmation step). Strains exhibiting an MIC &gt;1 mg/L were considered as non-susceptible (ns). We used a national 2013 strain collection to confirm the resistance in a particular genogroup. The genetic support of CFDC non-susceptibility was studied according to a collection of previously selected CFDC mutants.</div></div><div><h3>Results</h3><div>Six of 154 Sm strains (4%) were non-susceptible to CFDC, including one environmental strain and five clinical strains. None of the patients was exposed to CFDC. The strains belonged to four different sequence type (ST) (ST87, <em>n</em> = 2; ST39, <em>n</em> = 2; ST18, <em>n</em> = 1; and unknown, <em>n</em> = 1) but to the same genogroup (genogroup 4). All seven 2013 genogroup 4 strains were also non-susceptible to CFDC. None of the previously published mutations/deletions were identified, but common non-synonymous mutations were found in <em>ton</em>B and <em>tol</em>Q; a common polymorphism was identified in the promoter region of <em>smet</em>.</div></div><div><h3>Discussion</h3><div>The natural intrinsic non-susceptibility of the genogroup 4 could hamper the contribution of CFDC for the empiric treatment of Sm infections.</div></div>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":"31 10","pages":"Pages 1691-1696"},"PeriodicalIF":8.5,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yellow fever in South America in 2025: a concerning increase, but a true resurgence?","authors":"Aleksandra Barac, Nitin Gupta, Pikka Jokelainen, Sotirios Tsiodras, Marta Mora-Rillo, Effrossyni Gkrania-Klotsas, José Ramón Paño-Pardo, Casandra Bulescu, Galadriel Pellejero-Sagastizabal, Abraham Goorhuis, Maria Paquita Garcia Mendoza, Jacob Van der Ende, François-Xavier Lescure, Martin P Grobusch, Jan Felix Drexler","doi":"10.1016/j.cmi.2025.06.036","DOIUrl":"10.1016/j.cmi.2025.06.036","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicry of malignancy: peritoneal nodule caused by ectopic Enterobius vermicularis.","authors":"Alicia Moreno-Sabater, Vénus Tostivint, Adrien Pecriaux, Yann Parc","doi":"10.1016/j.cmi.2025.06.034","DOIUrl":"10.1016/j.cmi.2025.06.034","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of long-term sequelae following respiratory virus infections","authors":"Sharon H. Saydah,&nbsp;Fatimah S. Dawood","doi":"10.1016/j.cmi.2025.06.035","DOIUrl":"10.1016/j.cmi.2025.06.035","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":"31 9","pages":"Pages 1440-1442"},"PeriodicalIF":8.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of a low- and middle-income country site in the Staphylococcus aureus Network Adaptive Platform trial: experiences from Johannesburg.","authors":"Tom Boyles, Asha C Bowen, Rispah Chomba, Jeremy Nel, Joshua S Davis, Steven Y C Tong","doi":"10.1016/j.cmi.2025.06.032","DOIUrl":"10.1016/j.cmi.2025.06.032","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scope and Aims of ESCMID Guidelines’ – Author’s reply","authors":"Luigia Scudeller ,&nbsp;Effrossyni Gkrania-Klotsas ,&nbsp;José Ramon Pano Pardo","doi":"10.1016/j.cmi.2025.06.023","DOIUrl":"10.1016/j.cmi.2025.06.023","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":"31 10","pages":"Pages 1756-1757"},"PeriodicalIF":8.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: 'Survival and quality-of-life in Mucormycosis' by Abdulkader et al.","authors":"Shouhui Yi","doi":"10.1016/j.cmi.2025.06.022","DOIUrl":"10.1016/j.cmi.2025.06.022","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the accuracy of a novel algorithm for group B Streptococcus detection in pregnant women and its impact on early-onset neonatal group B streptococcal infections","authors":"Laura Villa ,&nbsp;Belén Fernández-Colomer ,&nbsp;Javier Fernández ,&nbsp;José Adánez ,&nbsp;Cesar Acebes ,&nbsp;Montserrat Junco ,&nbsp;Fernando Vazquez","doi":"10.1016/j.cmi.2025.06.026","DOIUrl":"10.1016/j.cmi.2025.06.026","url":null,"abstract":"<div><h3>Objectives</h3><div>The objective of this study was to assess the accuracy of a novel algorithm—designed in accordance with guidelines from the American College of Obstetricians and Gynecologists and the American Society for Microbiology—for detecting group B <em>Streptococcus</em> (GBS) colonization in pregnant women. Additionally, the study aimed to determine the potential impact of this algorithm on early-onset neonatal group B streptococcal disease (GBS-EOD) cases.</div></div><div><h3>Methods</h3><div>From March 2021 to March 2024, 5034 vaginal-rectal samples were collected from pregnant women at 35 weeks to 37 weeks gestation using ESwab™ medium. Samples were cultured in Todd Hewitt broth, subcultured on Granada medium, and tested with the DiaSorin Simplexa™ GBS Direct Assay (positive if Cycle threshold ≤ 35). Antimicrobial susceptibility testing was conducted on GBS isolates from penicillin-allergic women. GBS-positive women received intrapartum antibiotic prophylaxis. The study also reviewed cases of GBS-EOD during the same period.</div></div><div><h3>Results</h3><div>In total, 938 (18.6%) pregnant women were colonized with GBS. Among these, 625 samples (66.6%) were culture-positive, whereas 313 (33.4%) were culture-negative, but broth-enriched nucleic acid amplification test (NAAT)-positive. No samples were broth-enriched NAAT-negative and culturepositive. The broth-enriched NAAT demonstrated a sensitivity of 100 (95% CI: 99—100) and a specificity of 93 (95% CI: 92—94), along with a positive predictive value of 67 (95% CI: 67—70) and a negative predictive value of 100 (95% CI: 100—100). There was substantial agreement between methods (Cohen's kappa = 0.76 [95% CI: 0.74—0.79]). All 45 GBS isolates from penicillin-allergic women were susceptible to penicillin, ampicillin, and vancomycin, whereas 44.4% (20/45) were resistant to erythromycin, and 40% (18/45) were resistant to clindamycin. No cases of GBS-EOD were detected among the 5563 live newborns during the study period (95% CI: 0—0.054%).</div></div><div><h3>Discussion</h3><div>The novel algorithm was rapid, sensitive, and specific, effectively identifying candidates for intrapartum antibiotic prophylaxis, potentially reducing newborn infection and lowering GBS-EOD incidence and neonatal mortality rates. Integrating broth-enriched NAATs to verify negative culture samples could enhance detection and prevention efforts.</div></div>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":"31 10","pages":"Pages 1679-1683"},"PeriodicalIF":8.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}