Clinical Microbiology and Infection最新文献

{"title":"Re: 'Microbes know no borders: importation of macrolide-resistant Bordetella pertussis into France in 2024' by Bouchez et al.","authors":"Siyu Chen, Leran He, Kaihu Yao","doi":"10.1016/j.cmi.2025.07.017","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.017","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond guidelines: what do I need to know when dealing with fungal diagnostics?","authors":"Cornelia Lass-Flörl","doi":"10.1016/j.cmi.2025.07.016","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.016","url":null,"abstract":"<p><strong>Background: </strong>Diagnosing invasive fungal infections (IFIs) is notoriously challenging. Test sensitivity and specificity vary with fungal burden, overlapping microscopic fungal appearances, lack of quantitative culture thresholds and, above all, the patient's immune status, specimen quality and prior antifungal exposure. These variables can mask or mimic disease, leading to delayed or erroneous treatment decisions.</p><p><strong>Objective: </strong>To distil the practical, \"beyond-guideline\" insights that clinicians and laboratorians need for reliable IFI diagnosis, and to outline a context-driven approach that complements existing recommendations.</p><p><strong>Sources: </strong>Narrative synthesis of PubMed-indexed literature on fungal diagnostics published chiefly between 2020 - 2025, augmented by current global guidelines from ECMM/ISHAM/ASM and selected landmark papers outside this window when foundational.</p><p><strong>Content: </strong>The review explains how (i) host immune state (neutropenia, AIDS, corticosteroid use) skews antigen-versus-antibody performance; (ii) specimen choice (BAL, blood, CSF, tissue) and site of disease dictate test yield; (iii) pre-emptive or empiric antifungals suppress culture and antigen signals yet may leave PCR positive (iv) cross-reactivity (e.g., β-D-glucan with bacteremia, galactomannan with Fusarium); and mixed infections cloud interpretation; and (v) colonisation must be separated from invasion through combined microbiology, and clinical risk assessment. Traditional microscopy/culture, antigen assays, PCR and emerging next-generation sequencing are compared across major pathogen groups, with tables summarising sensitivities, specificities and pitfalls.</p><p><strong>Implications: </strong>Applying an approach that layers multiple modalities according to patient risk and specimen type can shorten time-to-diagnosis, reduce false negatives/positives and enable earlier targeted therapy. Integrating these context-aware steps into routine practice and future guideline updates is likely to improve IFI outcomes and optimise antifungal stewardship.</p>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re: 'Diagnostic accuracy of self-collected tongue swabs for Mycobacterium tuberculosis complex detection in individuals being assessed for TB in South Africa using the Xpert MTB/RIF Ultra assay' by David et al.","authors":"Salvatore Rotundo, Francesca Serapide, Alessandro Russo","doi":"10.1016/j.cmi.2025.07.018","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.018","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":8.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated Mycobacterium avium intracellulare complex infection.","authors":"Ke Xiao","doi":"10.1016/j.cmi.2025.07.014","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.014","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diversity of carbapenemases in Pseudomonas aeruginosa: a global genomic epidemiology investigation.","authors":"Jiayang Li, Jiajie Wang, Wenqi Wu, Ze Li, Xiuwen Wu, Jianan Ren","doi":"10.1016/j.cmi.2025.07.015","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.015","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and tolerability of Linezolid use in 186 children: A single centre observational study.","authors":"Neal Russell, Lucy Jefferson, Orlagh McGarrity, James Hatcher, Seilesh Kadambari, Hanna Schmid","doi":"10.1016/j.cmi.2025.07.013","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.013","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicentre evaluation of a EUCAST-based agar-screening method for terbinafine and itraconazole susceptibility of Trichophyton spp.","authors":"Joseph Meletiadis, Maria Siopi, Karin Meinike Jørgensen, Pilar Escribano, Henrich van de Lee, Jochem B Buil, Nathalie Friberg, Jesus Guinea, Maiken Cavling Arendrup","doi":"10.1016/j.cmi.2025.07.012","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.012","url":null,"abstract":"<p><strong>Objectives: </strong>Resistance in Trichophyton species has become a global public health issue. Here, a 4-well agar-screening method based on the EUCAST E.Def 10.2 method was evaluated in five centres using a panel of terbinafine wildtype (WT) and non-WT Trichophyton isolates with the recently determined tentative ECOFFs of the EUCAST E.Def 11.0 method.</p><p><strong>Methods: </strong>Forty-two Trichophyton isolates, all WT to itraconazole, 17 molecularly characterised terbinafine non-WT and 25 WT (non-WT/WT: 11/9 T. rubrum, 5/6 T. indotineae, 1/6 T. interdigitale and 0/4 T. mentagrophytes) were tested in five centres using 4-well plates containing terbinafine 0.016 mg/L and 0.125 mg/L; itraconazole 1 mg/L and drug-free agar, respectively. Plates were inoculated (25 μL, 0.5 McFarland) and incubated for 5-7 days at 25-28 °C. Visual growth comparable to drug-free control ignoring faint growth/pinpoint colonies indicated non-WT phenotype. Sensitivity and specificity in detecting Trichophyton non-WT isolates were calculated.</p><p><strong>Results: </strong>Most isolates produced sufficient growth after 5d whereas 6-10/42 required 7d of incubation. All isolates were correctly classified as WT to itraconazole by all 5 centres. The sensitivity (median (range among centres)) in detecting terbinafine non-WT isolates was 94%-100% (95% confidence interval 79%-100%) whereas the specificity for detecting WT isolates was 100%. Sensitivity and specificity were high across different species. Among the discrepancies, 1 false WT was observed with a T. rubrum strong mutant in 1 centre. WT T. indotineae grew on terbinafine 0.016 mg/L.</p><p><strong>Conclusions: </strong>The multicentre evaluation confirmed that the agar-screening method was sensitive and specific for detecting terbinafine non-WT Trichophyton isolates and correctly identified itraconazole WT strains.</p>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining Bloodstream and Central Venous Catheter-Related Infections in Patients Following Haematopoietic Cell Transplantation: Position Paper of the EBMT Infectious Diseases Working Party and Practice Harmonization and Guidelines Committee.","authors":"Dina Averbuch, Malgorzata Mikulska, Jan Styczynski, Anne Bergeron, Simone Cesaro, Raffaella Greco, Dionysios Neofytos, Francesco Onida, José Luis Piñana, Isabel Sanchez-Ortega, Paul E Verweij, Ibrahim Yakoub-Agha, Rafael de la Camara, Per Ljungman","doi":"10.1016/j.cmi.2025.06.037","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.06.037","url":null,"abstract":"<p><strong>Scope: </strong>This position paper is intended for clinicians and data managers involved in the diagnosis, management, and reporting of bloodstream (BSI) and central venous catheter (CVC)-related BSI (CRBSI) and CVC-related local skin/soft-tissue infections (lSSTI) in haematopoietic cell transplant (HCT) recipients.</p><p><strong>Methods: </strong>The panel reviewed the relevant guidelines on BSI and CVC-related lSSTI definitions, and their applicability to HCT recipients. We developed practical recommendations aiming to establish their standardized reporting considering the unique features of HCT recipients.</p><p><strong>Questions addressed by the position paper: </strong>Primary BSI definitionsAdequate blood volume is crucial for correct sampling. Definite BSI is defined as non-commensal pathogen identified by one positive culture or non-culture-based test; or two separate positive blood cultures with commensal pathogen, accompanied by any clinical or inflammatory markers deterioration. One positive blood culture set with viridans-group Streptococci accompanied by clinical or inflammatory markers deterioration is defined as a probable BSI.Following the validity confirmation, each primary BSI should be assessed for being a CRBSI and/or mucosal barrier injury (MBI) BSI; this terminology is not exclusive. CVC is considered a definite BSI source when the same pathogen grows from the cultures obtained from the CVC tip/hub and peripheral blood; confirmed by the differential time-to-positivity test or quantitative criteria. MBI is considered a source when intestinal pathogen grows in a patient with appropriate clinical context.CVC-related lSSTI definitionsCVC-related lSSTI (e.g. exit site, tunnel infection) should be defined as clinically or microbiologically documented (or both).Definition of recurrence and attributable mortalityRelapse should be differentiated from reinfection when reporting recurrence.We propose reporting 30-days mortality after BSI. Attributable mortality is defined if death directly attributed to BSI by the treating physician assessment.Uniform definition and reporting of BSI types will improve the analysis of their rates, related outcomes, and efficacy of preventative and treatment measures.</p>","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"War on AMR: High MDR carriage rates among war-injured Ukrainian refugees.","authors":"Tuomas Aro, Tuuve A Häkkinen, Ville Holmberg, Mikael Kajova, Anu Kantele","doi":"10.1016/j.cmi.2025.07.010","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.010","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice bodies in Brucellar tenosynovitis: a case report.","authors":"Jiahao Hao, Yumei Guo, Fangyu Qian, Weili Gao, Guangxia Liu, Conghui Cai, Huifen Zuo, Minghui Song, Jiaqing Ye, Chenfeng Zhang, Zhenjun Zhao, Yali Xu, Lijie Zhang","doi":"10.1016/j.cmi.2025.07.011","DOIUrl":"https://doi.org/10.1016/j.cmi.2025.07.011","url":null,"abstract":"","PeriodicalId":10444,"journal":{"name":"Clinical Microbiology and Infection","volume":" ","pages":""},"PeriodicalIF":10.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}