Clinical Diagnostic Laboratory Immunology最新文献_第8页

Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus 细胞培养法和贝壳瓶直接免疫过氧化物酶染色法检测单纯疱疹病毒和细胞自旋直接免疫荧光法检测水痘-带状疱疹病毒的比较

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-09-01 DOI: 10.1128/CDLI.8.5.909-912.2001

E. Chan, K. Brandt, G. Horsman

{"title":"Comparison of Chemicon SimulFluor Direct Fluorescent Antibody Staining with Cell Culture and Shell Vial Direct Immunoperoxidase Staining for Detection of Herpes Simplex Virus and with Cytospin Direct Immunofluorescence Staining for Detection of Varicella-Zoster Virus","authors":"E. Chan, K. Brandt, G. Horsman","doi":"10.1128/CDLI.8.5.909-912.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.5.909-912.2001","url":null,"abstract":"ABSTRACT A new rapid direct immunofluorescence assay, the SimulFluor direct fluorescent-antibody (DFA) assay, which can simultaneously detect herpes simplex virus types 1 and 2 (HSV-1 and -2) and varicella-zoster virus (VZV), was evaluated in comparison with our current standard procedures of (i) shell vial direct immunoperoxidase (shell vial IP) staining and cell culture for detection of HSV and (ii) cytospin DFA staining for VZV detection. A total of 517 vesicular, oral, genital, and skin lesion specimens were tested by all three procedures. For HSV detection, the SimulFluor DFA assay had an overall sensitivity, specificity, positive predictive value, and negative predictive value of 80.0, 98.3, 92.3, and 95.1%, respectively, when compared to culture. Shell vial IP staining had a sensitivity, specificity, positive predictive value, and negative predictive value of 87.6, 100, 100, and 96.9%, respectively, when compared with cell culture. The SimulFluor DFA assay, however, offers same-day, 1.5-hours results versus a 1- to 2-day wait for shell vial IP staining results and a 1- to 6-day wait for culture results for HSV. For VZV detection SimulFluor DFA staining detected 27 positive specimens as compared to 31 by our standard cytospin DFA technique—a correlation of 87.1%. A positive SimulFluor reaction for VZV is indicated by yellow-gold fluorescence compared to the bright apple-green fluorescence observed by cytospin DFA staining. There is no difference in turnaround time between the two assays. The SimulFluor DFA assay is a rapid immunofluorescence assay that can detect 80% of the HSV-positive specimens and 87% of the VZV-positive specimens with a 1.5-h turnaround time.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"44 1","pages":"909 - 912"},"PeriodicalIF":0.0,"publicationDate":"2001-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87532979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

AhpC, AhpD, and a Secreted 14-Kilodalton Antigen fromMycobacterium avium subsp.paratuberculosis Distinguish between Paratuberculosis and Bovine Tuberculosis in an Enzyme-Linked Immunosorbent Assay AhpC、AhpD和禽分枝杆菌14千道尔顿分泌抗原。在酶联免疫吸附试验中区分副结核和牛结核

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.08.4.797-801.2001

I. Olsen, M. Tryland, H. Wiker, L. Reitan

{"title":"AhpC, AhpD, and a Secreted 14-Kilodalton Antigen fromMycobacterium avium subsp.paratuberculosis Distinguish between Paratuberculosis and Bovine Tuberculosis in an Enzyme-Linked Immunosorbent Assay","authors":"I. Olsen, M. Tryland, H. Wiker, L. Reitan","doi":"10.1128/CDLI.08.4.797-801.2001","DOIUrl":"https://doi.org/10.1128/CDLI.08.4.797-801.2001","url":null,"abstract":"ABSTRACT Sera from cattle naturally infected with Mycobacterium avium subsp. paratuberculosis(n = 56) and naturally (n = 4) and experimentally (n = 8) infected withMycobacterium bovis were tested for the presence of antibodies against paratuberculosis antigens. An enzyme-linked immunosorbent assay (ELISA) was established based on absorption ofM. avium subsp. paratuberculosis antigens on a hyperimmune antiserum against M. avium subsp.avium proteins in order to remove cross-reacting antigens. This absorbed-antigen ELISA recognized 66% of animals with paratuberculosis (37 of 56), while none of the animals with naturally occurring bovine tuberculosis (TB) had detectable antibodies. However, the animals with experimental bovine TB also responded in this ELISA. Similar results were found in a commercial ELISA, showing that neither of these tests was able to distinguish between paratuberculosis and bovine TB. The sera were further tested for antibody activities against purified AhpC and AhpD, which are proteins constitutively expressed byM. avium subsp. paratuberculosis, and against a secreted 14-kDa protein present in culture filtrates from theM. avium complex. Elevated antibody levels to AhpC, AhpD, and the 14-kDa antigen were found in 27% (13 of 48), 15% (7 of 48), and 27% (13 of 48), respectively, of the cattle with paratuberculosis. Together these ELISAs were positive with 35% (17 of 48) of the animals. None of the animals with bovine TB had detectable antibodies against any of the purified proteins despite their high levels of cross-reacting antibodies. These results show that purified specific antigens are needed to differentiate between paratuberculosis and bovine TB in ELISA.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"44 1","pages":"797 - 801"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73781660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Celiac Disease-Associated Autoimmune Endocrinopathies 乳糜泻相关的自身免疫性内分泌疾病

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.678-685.2001

Vijay Kumar, M. Rajadhyaksha, J. Wortsman

{"title":"Celiac Disease-Associated Autoimmune Endocrinopathies","authors":"Vijay Kumar, M. Rajadhyaksha, J. Wortsman","doi":"10.1128/CDLI.8.4.678-685.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.678-685.2001","url":null,"abstract":"ABSTRACT Celiac disease (CD) is an autoimmune disorder induced by gluten intake in genetically susceptible individuals. It is characterized by the presence of serum antibodies to endomysium, reticulin, gliadin, and tissue transglutaminase. The incidence of CD in various autoimmune disorders is increased 10- to 30-fold in comparison to the general population, although in many cases CD is clinically asymptomatic or silent. The identification of such cases with CD is important since it may help in the control of type I diabetes or endocrine functions in general, as well as in the prevention of long-term complications of CD, such as lymphoma. It is believed that CD may predispose an individual to other autoimmune disorders such as type I diabetes, autoimmune thyroid, and other endocrine diseases and that gluten may be a possible trigger. The onset of type I diabetes at an early age in patients with CD, compared to non-CD, and the prevention or delay in onset of diabetes by gluten-free diet in genetically predisposed individuals substantiates this antigen trigger hypothesis. Early identification of CD patients in highly susceptible population may result in the treatment of subclinical CD and improved control of associated disorders.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"63 1","pages":"678 - 685"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75022644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 94

Cross-Reactivity of Epstein-Barr Virus-Specific Immunoglobulin M Antibodies with Cytomegalovirus Antigens Containing Glycine Homopolymers eb病毒特异性免疫球蛋白M抗体与含甘氨酸均聚物巨细胞病毒抗原的交叉反应性

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.747-756.2001

D. Lang, R. Vornhagen, M. Rothe, W. Hinderer, H. Sonneborn, B. Plachter

{"title":"Cross-Reactivity of Epstein-Barr Virus-Specific Immunoglobulin M Antibodies with Cytomegalovirus Antigens Containing Glycine Homopolymers","authors":"D. Lang, R. Vornhagen, M. Rothe, W. Hinderer, H. Sonneborn, B. Plachter","doi":"10.1128/CDLI.8.4.747-756.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.747-756.2001","url":null,"abstract":"ABSTRACT Timely and reliable detection of acute primary human cytomegalovirus (HCMV) infection is important in prenatal screening programs and for differential diagnosis of infectious mononucleosis-like disease. Enzyme-linked immunosorbent assays (ELISAs) based on HCMV proteins enable the sensitive detection of immunoglobulin M (IgM) antibodies during primary infection. However, concerns have been raised about possible cross-reactivities of the HCMV antigens used for the design of such ELISAs with IgM antibodies induced by Epstein-Barr Virus (EBV). In this study we investigated whether IgM antibodies generated during acute EBV infection reacted with recombinant HCMV antigens. Serum samples from patients with primary EBV infection frequently scored positive when tested in different HCMV IgM ELISAs, irrespective of whether conventional or recombinant antigens were used for the design of the HCMV IgM assays. Such cross-reactive IgM antibodies were found to be directed against short glycine-rich motifs contained within the nonstructural HCMV proteins pUL44 and pUL57. Further analyses revealed that these glycine-rich motifs were major antigenic domains for IgM antibodies induced during HCMV infection. Their deletion from recombinant proteins abrogated reactivity with IgM synthesized during HCMV infection. EBV-induced IgM antibodies that reacted with HCMV antigens showed similar kinetics of reactivity in HCMV- or EBV-specific assays in the course of primary EBV infection, indicating that the two populations of antibodies were highly overlapping. The results demonstrate that primary EBV infection leads to the induction of IgM antibodies that specifically bind to widely used diagnostic antigens of HCMV. This has to be considered in the interpretation of HCMV-specific IgM assays.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"150 1","pages":"747 - 756"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76411457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Generation and Serological Characterization of Murine Monoclonal Antibodies against O Antigens from Acinetobacter Reference Strains 小鼠抗不动杆菌参考株O抗原单克隆抗体的制备及血清学特性研究

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.825-827.2001

R. Pantophlet, L. Brade, H. Brade

引用次数: 11

Comparison of Hepatitis C Viral Loads in Patients with or without Human Immunodeficiency Virus 携带或不携带人类免疫缺陷病毒的丙型肝炎病毒载量的比较

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.690-694.2001

J. Matthews-Greer, G. Caldito, Sharon Adley, R. Willis, Angela C. Mire, R. Jamison, K. McRae, J. King, Wun-ling Chang

{"title":"Comparison of Hepatitis C Viral Loads in Patients with or without Human Immunodeficiency Virus","authors":"J. Matthews-Greer, G. Caldito, Sharon Adley, R. Willis, Angela C. Mire, R. Jamison, K. McRae, J. King, Wun-ling Chang","doi":"10.1128/CDLI.8.4.690-694.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.690-694.2001","url":null,"abstract":"ABSTRACT A better understanding of how human immunodeficiency virus (HIV) coinfection affects the course of hepatitis C virus (HCV) infection is required to select patients with HIV who would benefit from current HCV therapy. Between June 1996 and March 2000, HCV RNA levels were quantified for 1,279 patients at the Louisiana State University Health Sciences Center; 28 of these patients were coinfected with HIV. HCV loads were quantified by the Bayer branched-DNA assay with a lower limit of detection of 0.2 Meq/ml. We compared the median HCV RNA levels of for patients coinfected with HIV and HCV and patients infected only with HCV who were in the same age range (23 to 55 years). The median HCV load for the 28 patients coinfected with HCV and HIV (17.8 Meq/ml) was significantly greater (P < 0.05) than that for similarly aged patients infected only with HCV (6.1 Meq/ml). The HCV load did not correlate with age or sex for either group of patients. A significant (R = −0.4; P < 0.05) negative correlation was observed between HCV load and CD4 count in the coinfected group, for whom the CD4 counts at the time of HCV load analysis ranged from 6 to 1,773/mm3. The increased HCV load in patients coinfected with HCV and HIV compared to that in patients infected only with HCV and the inverse relationship of the HCV load to the CD4 count indicate that immunosuppression results in decreased control of HCV replication. In addition, we report significantly higher HCV loads among coinfected African Americans than Caucasians.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"43 1","pages":"690 - 694"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74745275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

groEL Encodes a Highly Antigenic Protein in Burkholderia pseudomallei groEL编码假马氏伯克氏菌高抗原蛋白

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.832-836.2001

Patrick C Y Woo, P. K. Leung, S. Wong, P. Ho, K. Yuen

{"title":"groEL Encodes a Highly Antigenic Protein in Burkholderia pseudomallei","authors":"Patrick C Y Woo, P. K. Leung, S. Wong, P. Ho, K. Yuen","doi":"10.1128/CDLI.8.4.832-836.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.832-836.2001","url":null,"abstract":"ABSTRACT No recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of thegroEL gene, which encodes an immunogenic protein ofBurkholderia pseudomallei. Bidirectional DNA sequencing ofgroEL revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by groEL is homologous to the chaperonins encoded by the groEL genes of other bacteria. It has 98% amino acid identity with the GroEL ofBurkholderia cepacia, 98% amino acid identity with the GroEL of Burkholderia vietnamiensis, and 82% amino acid identity with the GroEL of Bordetella pertussis. Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"60 1","pages":"832 - 836"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73268008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Spinal Cord Involvement in Uncomplicated Herpes Zoster 单纯带状疱疹的脊髓受累

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.850-851.2001

I. Steiner, B. Steiner-Birmanns, N. Levin, K. Hershko, I. Korn‐Lubetzki, I. Biran

引用次数: 14

Helicobacter pylori Intrafamilial Infections: Change in Source of Infection of a Child from Father to Mother after Eradication Therapy 幽门螺杆菌家族内感染:根除治疗后儿童从父亲到母亲感染源的变化

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.731-739.2001

I. Taneike, Y. Tamura, Toshiaki Shimizu, Y. Yamashiro, Tatsuo Yamamoto

{"title":"Helicobacter pylori Intrafamilial Infections: Change in Source of Infection of a Child from Father to Mother after Eradication Therapy","authors":"I. Taneike, Y. Tamura, Toshiaki Shimizu, Y. Yamashiro, Tatsuo Yamamoto","doi":"10.1128/CDLI.8.4.731-739.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.731-739.2001","url":null,"abstract":"ABSTRACT Biopsy specimens of the antrum and corpus were obtained from fourHelicobacter pylori-infected members of a family and from the same boy (son 1) in whom the infection reappeared after simultaneous successful eradication treatment of three family members, excluding the mother. A total of 18 to 60 H. pyloriisolates were obtained from each specimen and subjected to rRNA gene restriction pattern analysis. The father's isolates and the initial isolates from son 1 showed the same HindIII type, which was divided into three HaeIII subtypes. Isolates from the mother and a brother (son 2) and posttreatment isolates from son 1 showed a distinct HindIII type (with one minor subtype), which was divided into six HaeIII subtypes. All subtypes of the initial isolates from son 1 were present in the father's isolates, and all subtypes of the posttreatment isolates from son 1 were present in the mother's isolates but not in son 2's. Electron microscopic analysis of the biopsy specimens demonstrated extremely high levels ofH. pylori colonization in the father's gastric mucosa.H. pylori adherence with a ruffle formation was also demonstrated. The findings suggest that son 1 was infected initially with the H. pylori strain of the father and son 2 was infected with the H. pylori strain of the mother and that after eradication therapy son 1 was reinfected with the H. pylori strain of the mother, who did not undergo eradication therapy.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"105 1","pages":"731 - 739"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80692212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Flow Cytometric Microsphere-Based Immunoassay: Analysis of Secreted Cytokines in Whole-Blood Samples from Asthmatics 基于流式细胞微球的免疫分析:哮喘患者全血样本中分泌细胞因子的分析

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.776-784.2001

C. Camilla, L. Mély, A. Magnan, B. Casano, S. Prato, S. Debono, Felix Montero, J. Defoort, Marie Martin, V. Fert

{"title":"Flow Cytometric Microsphere-Based Immunoassay: Analysis of Secreted Cytokines in Whole-Blood Samples from Asthmatics","authors":"C. Camilla, L. Mély, A. Magnan, B. Casano, S. Prato, S. Debono, Felix Montero, J. Defoort, Marie Martin, V. Fert","doi":"10.1128/CDLI.8.4.776-784.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.776-784.2001","url":null,"abstract":"ABSTRACT The ability of flow cytometry to resolve multiple parameters was used in a microsphere-based flow cytometric assay for the simultaneous determination of several cytokines in a sample. The flow cytometer microsphere-based assay (FMBA) for cytokines consists of reagents and dedicated software, specifically designed for the quantitative determination of cytokines. We have made several improvements in the multiplex assay: (i) dedicated software specific for the quantitative multiplex assay that processes data automatically, (ii) a stored master calibration curve with a two-point recalibration to adjust the stored curve periodically, and (iii) an internal standard to normalize the detection step in each sample. Overall analytical performance, including sensitivity, reproducibility, and dynamic range, was investigated for interleukin-4 (IL-4), IL-6, IL-10, IL-12, gamma interferon (IFN-γ), and tumor necrosis factor alpha. These assays were found to be reproducible and accurate, with a sensitivity in the picograms-per-milliliter range. Results obtained with FMBA correlate well with commercial enzyme-linked immunosorbent assay data (r > 0.98) for all cytokines assayed. This multiplex assay was applied to the determination of cytokine profiles in whole blood from atopic and nonatopic patients. Our results show that atopic subjects' blood produces more IL-4 (P = 0.003) and less IFN-γ (P = 0.04) than the blood of nonatopic subjects. However, atopic asthmatic subjects' blood produces significantly more IFN-γ than that of atopic nonasthmatic subjects (P = 0.03). The results obtained indicate that the FMBA technology constitutes a powerful system for the quantitative, simultaneous determination of secreted cytokines in immune diseases.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"36 1","pages":"776 - 784"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82827750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42