Clinical Diagnostic Laboratory Immunology最新文献_第9页

Immune Function in Healthy Inner-City Children 健康城市儿童的免疫功能

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.740-746.2001

J. A. Bartlett, A. Goldklang, S. Schleifer, S. Keller

引用次数: 23

Cytopathic Changes in Rat Microglial Cells Induced by Pathogenic Acanthamoeba culbertsoni: Morphology and Cytokine Release 致病性卡伯氏棘阿米巴诱导大鼠小胶质细胞病变:形态学和细胞因子释放

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.837-840.2001

Ho-Joon Shin, M. Cho, Suk-Yul Jung, Hyung-Il Kim, Sun Park, J. Seo, Jung-Chil Yoo, K. Im

{"title":"Cytopathic Changes in Rat Microglial Cells Induced by Pathogenic Acanthamoeba culbertsoni: Morphology and Cytokine Release","authors":"Ho-Joon Shin, M. Cho, Suk-Yul Jung, Hyung-Il Kim, Sun Park, J. Seo, Jung-Chil Yoo, K. Im","doi":"10.1128/CDLI.8.4.837-840.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.837-840.2001","url":null,"abstract":"ABSTRACT To determine whether pathogenic Acanthamoeba culbertsoni trophozoites and lysate can induce cytopathic changes in primary-culture microglial cells, morphological changes were observed by transmission electron microscopy (TEM). In addition, the secretion of two kinds of cytokines, tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β), from microglial cells was observed. Trophozoites of pathogenic A. culbertsoni made contact with microglial cells and produced digipodia. TEM revealed that microglial cells cocultured with amoebic trophozoites underwent a necrotic process, accompanied by lysis of the cell membrane. TEM of microglial cells cocultured with amoebic lysate showed that the membranes of the small cytoplasmic vacuoles as well as the cell membrane were lysed. The amounts of TNF-α secreted from microglial cells cocultured with A. culbertsoni trophozoites or lysate increased at 6 h of incubation. The amounts of IL-1β secreted from microglial cells cocultured with A. culbertsonitrophozoites at 6 h of incubation was similar to those secreted from the control group, but the amounts decreased during cultivation with A. culbertsoni lysate. These results suggest that pathogenic A. culbertsoni induces the cytopathic effects in primary-culture rat microglial cells, with the effects characterized by necrosis of microglial cells and changes in levels of secretion of TNF-α and IL-1β from microglial cells.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"40 1","pages":"837 - 840"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80298288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Viral Load in Breast Milk Correlates with Transmission of Human Cytomegalovirus to Preterm Neonates, but Lactoferrin Concentrations Do Not 母乳中的病毒载量与人巨细胞病毒向早产儿的传播有关，但乳铁蛋白浓度无关

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.818-821.2001

B. V. D. van der Strate, M. Harmsen, P. Schäfer, P. Swart, T. The, G. Jahn, C. Speer, D. Meijer, K. Hamprecht

引用次数: 45

Biological Response Modifier Activity of an Exopolysaccharide from Paenibacillus jamilae CP-7 蚕豆芽孢杆菌CP-7胞外多糖生物反应调节剂活性的研究

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.706-710.2001

A. Ruiz-Bravo, M. Jiménez-Valera, E. Moreno, V. Guerra, A. Ramos‐Cormenzana

{"title":"Biological Response Modifier Activity of an Exopolysaccharide from Paenibacillus jamilae CP-7","authors":"A. Ruiz-Bravo, M. Jiménez-Valera, E. Moreno, V. Guerra, A. Ramos‐Cormenzana","doi":"10.1128/CDLI.8.4.706-710.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.706-710.2001","url":null,"abstract":"ABSTRACT An extracellular polysaccharide was purified from culture supernatants of Paenibacillus jamilae CP-7, a gram-positive bacillus that was isolated from compost prepared with olive mill wastewaters. The extracellular polysaccharide was produced under aerobic conditions in a medium containing olive mill wastewaters (80% [vol/vol]). This exopolymer had a low level of acute toxicity when it is administered to BALB/c mice by the intraperitoneal route. Interesting immunomodulatory effects were detected when mice were given 10 mg of exopolysaccharide per kg of body weight; the proliferative responses of splenocytes to B-cell and T-cell mitogens were suppressed, the in vitro levels of production of gamma interferon and granulocyte-macrophage colony-stimulating factor by unstimulated and lipopolysaccharide-stimulated splenocytes were enhanced, and the levels of resistance to the intracellular pathogen Listeria monocytogenes was increased in mice. Also, the exopolysaccharide was able to induce lymphocyte proliferation in vitro. We conclude thatP. jamilae produces an exopolysaccharide with interesting immunomodulatory properties.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"50 1","pages":"706 - 710"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82017731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Heartwater (Cowdria ruminantiumInfection) as a Cause of Postrestocking Mortality of Goats in Mozambique 心水(考德里亚反刍菌感染)是莫桑比克山羊放养后死亡的一个原因

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.843-846.2001

C. Bekker, D. Vink, Carlos M. Lopes Pereira, W. Wapenaar, A. Langa, F. Jongejan

引用次数: 23

Identification and Strain Differentiation of Vibrio cholerae by Using Polyclonal Antibodies against Outer Membrane Proteins 利用抗外膜蛋白多克隆抗体鉴定和分化霍乱弧菌

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.768-771.2001

A. Martínez-Govea, J. Ambrosio, L. Gutiérrez-Cogco, A. Flisser

引用次数: 27

Induction by a Lactic Acid Bacterium of a Population of CD4+ T Cells with Low Proliferative Capacity That Produce Transforming Growth Factor β and Interleukin-10 乳酸菌诱导低增殖能力的CD4+ T细胞产生转化生长因子β和白细胞介素-10

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.695-701.2001

T. von der Weid, C. Bulliard, E. Schiffrin

{"title":"Induction by a Lactic Acid Bacterium of a Population of CD4+ T Cells with Low Proliferative Capacity That Produce Transforming Growth Factor β and Interleukin-10","authors":"T. von der Weid, C. Bulliard, E. Schiffrin","doi":"10.1128/CDLI.8.4.695-701.2001","DOIUrl":"https://doi.org/10.1128/CDLI.8.4.695-701.2001","url":null,"abstract":"ABSTRACT We investigated whether certain strains of lactic acid bacteria (LAB) could antagonize specific T-helper functions in vitro and thus have the potential to prevent inflammatory intestinal immunopathologies. All strains tested induced various levels of both interleukin-12 (IL-12) and IL-10 in murine splenocytes. In particular,Lactobacillus paracasei (strain NCC2461) induced the highest levels of these cytokines. Since IL-12 and IL-10 have the potential to induce and suppress Th1 functions, respectively, we addressed the impact of this bacterium on the outcome of CD4+ T-cell differentiation. For this purpose, bacteria were added to mixed lymphocyte cultures where CD4+ T-cells from naive BALB/c mice were stimulated weekly in the presence of irradiated allogeneic splenocytes. In these cultures, L. paracasei NCC2461 strongly inhibited the proliferative activity of CD4+ T cells in a dose-dependent fashion. This was accompanied by a marked decrease of both Th1 and Th2 effector cytokines, including gamma interferon, IL-4, and IL-5. In contrast, IL-10 was maintained and transforming growth factor β (TGF-β) was markedly induced in a dose-dependent manner. The bacteria were not cytotoxic, because cell viability was not affected after two rounds of stimulation. Thus, unidentified bacterial components from L. paracasei NCC2461 induced the development of a population of CD4+ T cells with low proliferative capacity that produced TGF-β and IL-10, reminiscent of previously described subsets of regulatory cells implicated in oral tolerance and gut homeostasis.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"90 1","pages":"695 - 701"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82418202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 263

Enhancement of Splenic-Macrophage Fcγ Receptor Expression by Treatment with Estrogens 雌激素对脾巨噬细胞Fcγ受体表达的影响

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/cdli.8.4.806-810.2001

F. Gomez, P. Ruiz, J. A. Bernal, M. Escobar, A. Garcia-Egido, J. López-Sáez

{"title":"Enhancement of Splenic-Macrophage Fcγ Receptor Expression by Treatment with Estrogens","authors":"F. Gomez, P. Ruiz, J. A. Bernal, M. Escobar, A. Garcia-Egido, J. López-Sáez","doi":"10.1128/cdli.8.4.806-810.2001","DOIUrl":"https://doi.org/10.1128/cdli.8.4.806-810.2001","url":null,"abstract":"ABSTRACT Splenic-macrophage Fcγ receptors (FcγRs) participate in the pathophysiologies of immune-complex diseases and in host defense against infection. Modulation of macrophage FcγR expression is an immuno-therapeutic target. Glucocorticoids, sex steroids, and dopaminergic drugs modulate macrophage FcγR expression. Previous data indicate that estradiol increases macrophage FcγR expression. Nevertheless, the effects of clinically used estrogens upon macrophage FcγR expression are unknown. We assessed the effects of treatment with commonly used estrogens on the expression of macrophage FcγRs using a guinea pig experimental model. Six estrogens have been studied: ethynylestradiol (Et), mestranol (M), chlortianisene (Ct), promestriene, 17-epiestriol, and 17β-estradiol. Following in vivo treatment of guinea pigs, we determined the clearance of immunoglobulin G (IgG)-sensitized erythrocytes in vivo, the binding of IgG-sensitized erythrocytes by isolated splenic macrophages, and splenic-macrophage FcγR cell surface expression. Estrogens enhance the clearance of IgG-sensitized erythrocytes by increasing splenic-macrophage FcγR expression. Et, M, and Ct were more effective than the other estrogens. Flow cytometry and fluorescence microscopy with monoclonal antibodies demonstrated that estrogens increase the cell surface expression of FcγR1 and -2 more than that of FcγR2. These data indicate that treatment with commonly used estrogens enhances the clearance of IgG-sensitized cells by improving splenic-macrophage FcγR expression.","PeriodicalId":10395,"journal":{"name":"Clinical Diagnostic Laboratory Immunology","volume":"9 1","pages":"806 - 810"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87317521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Production of BartonellaGenus-Specific Monoclonal Antibodies 巴尔通体球菌特异性单克隆抗体的制备

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/cdli.8.4.847-849.2001

Zhongxing Liang, B. La Scola, H. Lepidi, D. Raoult

引用次数: 7

Real-Time PCR as a New Tool for QuantifyingLeishmania infantum in Liver in Infected Mice 实时荧光定量PCR检测幼年利什曼原虫感染小鼠肝脏的新方法

Clinical Diagnostic Laboratory Immunology Pub Date : 2001-07-01 DOI: 10.1128/CDLI.8.4.828-831.2001

S. Bretagne, R. Durand, M. Olivi, J. Garin, A. Sulahian, D. Rivollet, M. Vidaud, M. Deniau

引用次数: 119