Clinical and laboratory haematology最新文献_第8页

Evaluation of the automated leucocyte count and differential from the Cell-Dyn 3500 in sickle cell disease. 镰状细胞病自动白细胞计数和cell - dyn3500鉴别的评价

Clinical and laboratory haematology Pub Date : 1995-12-01

R E Joyner, M J Brooks

{"title":"Evaluation of the automated leucocyte count and differential from the Cell-Dyn 3500 in sickle cell disease.","authors":"R E Joyner, M J Brooks","doi":"","DOIUrl":"","url":null,"abstract":"Erythrocytes resistant to standard lysing reagents are known to occur in sickle cell disease. These lyse-resistant erythrocytes can cause aberrant automated leucocyte counts and differentials. The ability of the Cell-Dyn 3500 automated haematology analyser to eliminate resistant erythrocytes and accurately count and differentiate leucocytes was evaluated. Samples were obtained from paediatric patients with sickle cell disease or haemoglobin SC disease. The Cell-Dyn 3500, using impedance and optical counting with a hypotonic salt \"extended lyse mode', was compared to the Cell-Dyn 3000, an optical analyser that also uses a hypotonic salt lyse, the Cell-Dyn 400, a \"hard detergent lyse' impedance counter, and a reference 400-cell manual white cell differential (National committee for Clinical Laboratory Standards [NCCLS] Approved Guideline H20-A). Seventy-five samples from patients with sickle cell disease or haemoglobin SC disease were evaluated for total leucocyte count, percentage of lymphocytes, percentage of neutrophils, and nucleated red blood cells (NRBC) flags. The Cell-Dyn 3500 correlated well with Cell-Dyn 400 leucocyte counts, with a correlation coefficient of 0.95. When compared to the manual differential, the correlation coefficient for lymphocytes was 0.93 and for neutrophils 0.95. The Cell-Dyn 3500 NRBC flag had a sensitivity of 47.7% and a specificity of 80.6%. The predictive value of a positive flag was 77.7%. The Cell-Dyn 3500's extended lyse mode clearly enhances the accuracy of leucocyte counts and differentials in patients with sickle cell disease.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"17 4","pages":"329-33"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-splenectomy lymphocytosis. Post-splenectomy淋巴球增多。

Clinical and laboratory haematology Pub Date : 1995-12-01

S Juneja, E Januszewicz, M Wolf, I Cooper

引用次数: 0

Pseudoleucocytosis due to incomplete erythrocyte lysis. 由于红细胞不完全溶解引起的假性白血球增多症。

Clinical and laboratory haematology Pub Date : 1995-12-01

I Mellors, P McArdle, D Bell

引用次数: 0

The value of gel test and ELAT in autoimmune haemolytic anaemia. 凝胶试验和ELAT在自身免疫性溶血性贫血中的价值。

Clinical and laboratory haematology Pub Date : 1995-12-01

J Fabijanska-Mitek, H Namirska-Krzton, H Seyfried

{"title":"The value of gel test and ELAT in autoimmune haemolytic anaemia.","authors":"J Fabijanska-Mitek, H Namirska-Krzton, H Seyfried","doi":"","DOIUrl":"","url":null,"abstract":"Three methods for detection of warm type IgG autoantibody were evaluated using 400 blood samples from 147 patients suspected of autoimmune haemolytic anaemia (AIHA). Three direct antiglobulin techniques (DAT) were used: conventional tube DAT, gel-DAT by micromethod and gel-DAT enzyme linked antiglobulin test (ELAT). Eluate examinations confirmed the presence of autoantibodies on red cells. These tests were compared directly using 126 selected blood samples from 85 patients with IgG molecules on their red cells detected by the gel test. In 106 of these samples, collected from 65 patients with clinical symptoms of AIHA, the presence of autoantibody was confirmed by acid elution. The ELAT was positive in 100 samples (94%), 87 samples for tube DAT (82%). The ELAT as well as the tube DAT was negative in 20 samples with non-reactive eluates by gel test. The gel-DAT was therefore not fully specific and detected IgG on red cells of patients with hypergammaglobulinaemia. However, due its higher sensitivity it proved useful as a screening test. The ELAT allowed changes in the number of IgG molecules per red cell to be monitored quantitatively. Both methods play a part in the diagnosis and monitoring patients with warm type IgG auto-antibody.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"17 4","pages":"311-6"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential thrombocythaemia followed by multiple myeloma in the same patient. 原发性血小板增多症并发多发性骨髓瘤。

Clinical and laboratory haematology Pub Date : 1995-12-01

P R Kelsey, K Patel

引用次数: 0

Validation of an algorithm for oral anticoagulant dosing and appointment scheduling. 一种口服抗凝剂给药和预约调度算法的验证。

Clinical and laboratory haematology Pub Date : 1995-12-01

B D Vadher, D L Patterson, M S Leaning

{"title":"Validation of an algorithm for oral anticoagulant dosing and appointment scheduling.","authors":"B D Vadher, D L Patterson, M S Leaning","doi":"","DOIUrl":"","url":null,"abstract":"Computer clinical decision-support systems require validation before clinical use. This study compared recommendations on warfarin dosage adjustment and timing of the next appointment made by an algorithm with those made by experienced and inexperienced clinicians. Data abstracted from the records of 125 patients seen regularly in the anticoagulant clinic were used. The algorithm recommended dose changes and next appointment for cases with INRs between 1.8 to 4.2 (therapeutic range 2.0-3.0) and between 2.3 to 5.3 (therapeutic range 3.0-4.5). Beyond these values the algorithm referred the cases to \"see doctor'. Compared to experienced clinicians, the algorithm was better at \"recognising' difficult patients than inexperienced clinicians (kappa = 0.43 and 0.32 respectively). There was no statistically significant difference between all decision makers in dosage recommendations for the non-difficult cases, but there was much more variation amongst the inexperienced clinicians. The interval recommendations were statistically different between and within the different decision-makers. The inexperienced clinicians tended to give relatively longer intervals for a given dose change. In conclusion, the algorithm performs better than inexperienced clinicians and as well as experienced clinicians for the non-difficult cases.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"17 4","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19670645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating CD20dim T-lymphocytes increase with age: evidence for a memory cytotoxic phenotype. 循环CD20dim t淋巴细胞随年龄增加:记忆细胞毒性表型的证据。

Clinical and laboratory haematology Pub Date : 1995-12-01

I Storie, G A Wilson, V Granger, D Barnett, J T Reilly

引用次数: 0

Letter from America: quis custodiet ipsos custodes? 美国来信：quis custodiet ipsos custodes？

Clinical and laboratory haematology Pub Date : 1995-12-01

S A Bentley

引用次数: 0

Enzyme-linked antiglobulin test for the evaluation of the amount of IgG autoantibodies on red blood cells. 酶联抗球蛋白试验用于评价红细胞上IgG自身抗体的数量。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01240.X

H. Namirska-Krztoń, J. Fabijańska‐Mitek, H. Seyfried