Clinical and experimental immunology最新文献_第4页

The neutrophil extracellular traps in neurological diseases: an update. 神经系统疾病中的中性粒细胞胞外捕获器：最新进展。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-12 DOI: 10.1093/cei/uxae057

Xiaoping Yu, Zhaoyan Chen, Wei Bao, Yaqing Jiang, Fei Ruan, Di Wu, Kai Le

{"title":"The neutrophil extracellular traps in neurological diseases: an update.","authors":"Xiaoping Yu, Zhaoyan Chen, Wei Bao, Yaqing Jiang, Fei Ruan, Di Wu, Kai Le","doi":"10.1093/cei/uxae057","DOIUrl":"10.1093/cei/uxae057","url":null,"abstract":"Neutrophil extracellular traps (NETs) released by neutrophils are web-like DNA structures adhered to granulin proteins with bactericidal activity and can be an important mechanism for preventing pathogen dissemination or eliminating microorganisms. However, they also play important roles in diseases of other systems, such as the central nervous system. We tracked the latest advances and performed a review based on published original and review articles related to NETs and neurological diseases. Generally, neutrophils barely penetrate the blood-brain barrier into the brain parenchyma, but when pathological changes such as infection, trauma, or neurodegeneration occur, neutrophils rapidly infiltrate the central nervous system to exert their defensive effects. However, neutrophils may adversely affect the host when they uncontrollably release NETs upon persistent neuroinflammation. This review focused on recent advances in understanding the mechanisms and effects of NETs release in neurological diseases, and we also discuss the role of molecules that regulate NETs release in anticipation of clinical applications in neurological diseases.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":"264-274"},"PeriodicalIF":3.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating interferon type I responses in patients with suspected giant cell arteritis and polymyalgia rheumatica. 调查巨细胞动脉炎和多发性风湿痛疑似患者的 I 型干扰素反应。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-12 DOI: 10.1093/cei/uxae085

Marieke van Nieuwland, A H Leontine Mulder, Edgar M Colin, Celina Alves, Lenny van Bon, Elisabeth Brouwer

{"title":"Investigating interferon type I responses in patients with suspected giant cell arteritis and polymyalgia rheumatica.","authors":"Marieke van Nieuwland, A H Leontine Mulder, Edgar M Colin, Celina Alves, Lenny van Bon, Elisabeth Brouwer","doi":"10.1093/cei/uxae085","DOIUrl":"10.1093/cei/uxae085","url":null,"abstract":"Giant cell arteritis (GCA) and polymyalgia rheumatica (PMR) are closely related inflammatory disorders. Easily measurable biomarkers defining active disease and identifying patients in need of glucocorticoid sparing treatment options are highly desired. Interferon Type I (IFN-I) might be involved in disease pathology; however, evidence is limited. This study explores a systemic IFN-I signature and expression of IFN-I markers in GCA and PMR patients. Treatment naive GCA and PMR patients, and PMR patients with glucocorticoid treatment were included. Patients suspected of but not diagnosed with GCA were used as controls. Five relevant IFN-I-stimulated genes were identified in literature, and relative expression levels were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR) in peripheral blood mononuclear cells. An IFN-I score was generated. Serum levels of IFN-I induced C-X-C motif chemokine 10 (CXCL10) and Galectin-9 were determined by multiplex immunoassay. There were no differences in IFN-I scores between the groups. An IFN-I signature was observed in 0/9 controls, 2/11 GCA patients, 4/20 treatment naive PMR patients, and 2/10 PMR patients with treatment. Serum CXCL10 and Galectin-9 were not increased in GCA or PMR patients compared to control patients. Treated PMR patients had lower CXCL10 levels [423.2 pg/ml (375.1-491.1)] compared to treatment naive PMR patients [641.8 pg/ml (552.8-830.6)]. An IFN-I signature does not distinguish GCA and PMR patients from controls. Also, IFN-I-induced serum markers are not upregulated in GCA and PMR patients. Easily measurable IFN-I-induced serum markers will therefore probably not aid in diagnosis and additional treatment options in newly diagnosed GCA and PMR patients.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":"308-313"},"PeriodicalIF":3.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Cisplatin-mediated down-regulation of miR-145 contributes to up-regulation of PD-L1 via the c-Myc transcription factor in cisplatin-resistant ovarian carcinoma cells. 更正为顺铂介导的 miR-145 下调有助于顺铂耐药卵巢癌细胞通过 c-Myc 转录因子上调 PD-L1。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-07 DOI: 10.1093/cei/uxae091

引用次数: 0

Human FCHO1 deficiency - Review of the Literature and Additional Two Cases. 人类 FCHO1 缺乏症 - 文献综述和新增的两个病例。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-05 DOI: 10.1093/cei/uxae097

Cansu Özdemiral, Ismail Yaz, Saliha Esenboga, Nadira Nabiyeva Cevik, Hacer Neslihan Bildik, Mehmet Kilic, Ilhan Tezcan, Deniz Cagdas

{"title":"Human FCHO1 deficiency - Review of the Literature and Additional Two Cases.","authors":"Cansu Özdemiral, Ismail Yaz, Saliha Esenboga, Nadira Nabiyeva Cevik, Hacer Neslihan Bildik, Mehmet Kilic, Ilhan Tezcan, Deniz Cagdas","doi":"10.1093/cei/uxae097","DOIUrl":"https://doi.org/10.1093/cei/uxae097","url":null,"abstract":"F-BAR domain only protein 1(FCHO1) contributes as a critical component to an essential cellular process, clathrin-mediated endocytosis(CME). CME involves cellular membrane invagination followed by cargo protein recruitment and adaptor protein assembly to form endocytic vesicles, maintains several cellular functions, such as signaling, differentiation, nutrition, absorption, and secretion. We aimed to determine the clinical/immunological findings in FCHO1 deficiency to generate appropriate medical approach. We present clinical/immunological/genetic findings of two FCHO1 deficiency patients together with recently reported 17 patients. We found two different variants in the patients, one previously defined and one novel homozygous mutation(c.306C>A(p.Tyr102Ter)). Recurrent sinopulmonary infections occurred in all patients, with viral(63.1%) and fungal(52.6%) infections frequently reported. Lymphopenia and CD4+T cell lymphopenia are present in 77.7%(14/18) and 100% of patients, respectively. CD8+ T cell number is low in half. Hypogammaglobulinemia and low IgM are present in 83.3%(15/18) and 61.1%(11/18) of patients, respectively. Neurological disorders(Guillian-Barre Syndrome, Moya Moya disease, encephalitis, and cranial infarction) are common(n=6(31.5%)). Malignancy is present in four(21%) patients, three suffered from diffuse large B cell lymphoma and one developed Hodgkin lymphoma. Additional clinical and laboratory results from more patients helped to define the characteristics of FCHO1 deficiency. The early application of molecular genetic analysis in CID patients is crucial. Since all transplanted patients were alive, allogeneic hematopoietic stem cell transplantation emerged as a potential curative therapy.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The B-cells paradigm in Systemic Sclerosis: an update on pathophysiology and B-cell targeted therapies. 系统性硬化症中的 B 细胞范例：病理生理学和 B 细胞靶向疗法的最新进展。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-05 DOI: 10.1093/cei/uxae098

Cristina Scaletti, Sara Pratesi, Silvia Bellando Randone, Linda Di Pietro, Corrado Campochiaro, Francesco Annunziato, Marco Matucci Cerinic

{"title":"The B-cells paradigm in Systemic Sclerosis: an update on pathophysiology and B-cell targeted therapies.","authors":"Cristina Scaletti, Sara Pratesi, Silvia Bellando Randone, Linda Di Pietro, Corrado Campochiaro, Francesco Annunziato, Marco Matucci Cerinic","doi":"10.1093/cei/uxae098","DOIUrl":"https://doi.org/10.1093/cei/uxae098","url":null,"abstract":"Systemic sclerosis is considered a rare autoimmune disease in which there are alterations of both the innate and adaptive immune response resulting in the production of autoantibodies. Abnormalities of the immune system compromise the normal function of blood vessels leading to a vasculopathy manifested by Raynaud's phenomenon, an early sign of systemic sclerosis. As a consequence of this reactive picture, the disease can evolve leading to tissue fibrosis. Several systemic sclerosis-specific autoantibodies are currently known and are associated with specific clinical manifestations and prognosis. Although the pathogenetic role of these autoantibodies is still unclear, their production by B cells and plasma cells suggests the importance of these cells in the development of systemic sclerosis. This review narratively examines B cell dysfunctions and their role in the pathogenesis of systemic sclerosis and discusses B cell-targeted therapies currently used or potentially useful for the management of end-organ complications.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigen-specific T cell frequency and phenotype mirrors disease activity in DRB1*04:04+ rheumatoid arthritis patients. 抗原特异性 T 细胞频率和表型反映了 DRB1*04:04+ 类风湿性关节炎患者的疾病活动。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-04 DOI: 10.1093/cei/uxae102

Cliff Rims, Hannes Uchtenhagen, Kadin Brooks, Bernard Ng, Sylvia E Posso, Jeffrey Carlin, William W Kwok, Jane H Buckner, Eddie A James

{"title":"Antigen-specific T cell frequency and phenotype mirrors disease activity in DRB1*04:04+ rheumatoid arthritis patients.","authors":"Cliff Rims, Hannes Uchtenhagen, Kadin Brooks, Bernard Ng, Sylvia E Posso, Jeffrey Carlin, William W Kwok, Jane H Buckner, Eddie A James","doi":"10.1093/cei/uxae102","DOIUrl":"https://doi.org/10.1093/cei/uxae102","url":null,"abstract":"Rheumatoid arthritis (RA) is associated with high-risk HLA class II alleles known as the \"RA shared epitope.\" Among prevalent shared epitope alleles, study of DRB1*04:04 has been limited. To define relevant epitopes, we identified citrullinated peptide sequences from synovial antigens that were predicted to bind to HLA-DRB1*04:04 and utilized a systematic approach to confirm their binding and assess their recognition by CD4 T cells. After confirming the immunogenicity of 13 peptides derived from aggrecan, cartilage intermediate layer protein (CILP), α-enolase, vimentin, and fibrinogen, we assessed their recognition by T cells from a synovial tissue sample, observing measurable responses to 8 of the 13 peptides. We then implemented a multicolor tetramer panel to evaluate the frequency and phenotype of antigen-specific CD4 T cells in individuals with anti-citrullinated protein antibody (ACPA)-positive RA and controls. In subjects with RA, CILP-specific T cell frequencies were significantly higher than those of other antigens. The surface phenotypes exhibited by antigen-specific T cells were heterogeneous, but Th1-like and Th2-like cells predominated. Stratifying based on disease status and activity, antigen-specific T cells were more frequent and most strongly polarized in RA subjects with high disease activity. In total, these findings identify novel citrullinated epitopes that can be used to interrogate antigen-specific CD4 T cells and show that antigen-specific T cell frequency is elevated in subjects with high disease activity.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of vitamin D: a promising pathway to combat neuropsychiatric lupus disorders. 维生素 D 的作用：防治神经精神狼疮疾病的有效途径。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-11-04 DOI: 10.1093/cei/uxae099

Thaís Evelyn Karnopp, Gustavo Flores Chapacais, Maria Luísa Gasparini, Natália Garcia Dos Santos, Vinicius da Silva Freitas, Marina Piccoli, Andressa Leite Di Domenico, Lucas Denardi Doria, Nikolas Mateus Pereira de Souza, Alexandre Rieger, Eduarda Correa Freitas, Fernanda Visioli, Odirlei André Monticielo

{"title":"The role of vitamin D: a promising pathway to combat neuropsychiatric lupus disorders.","authors":"Thaís Evelyn Karnopp, Gustavo Flores Chapacais, Maria Luísa Gasparini, Natália Garcia Dos Santos, Vinicius da Silva Freitas, Marina Piccoli, Andressa Leite Di Domenico, Lucas Denardi Doria, Nikolas Mateus Pereira de Souza, Alexandre Rieger, Eduarda Correa Freitas, Fernanda Visioli, Odirlei André Monticielo","doi":"10.1093/cei/uxae099","DOIUrl":"https://doi.org/10.1093/cei/uxae099","url":null,"abstract":"To evaluate neuropsychiatric manifestations in the pristane-induced lupus (PIL) model, as well as to evaluate immunoregulatory effects of vitamin D (vit-D) in the brain of mice with PIL. Eighty female BALB/c mice were divided into six groups with 90 (3 months) and 180 (6 months) days of experimentation: CO3, CO6 (controls), PIL3, PIL6 (pristane-induced lupus), VD3 and VD6 (PIL supplemented with 1,25-dihydroxyvitamin D). Forced-swim, elevated plus maze and Barnes maze were the behavioral tests performed. Expression of pVDR was assessed by immunofluorescence. Brain IgM and IgG deposits were evaluated by double staining fluorescence. Serum IL-6 and IFN-α1 were quantified by ELISA. AUC-ROC curve was also performed for immunoglobulins. PIL and VD showed depressive-like behavior in the forced-swim test and anxious-like behavior in the elevated plus maze test. PIL also presented cognitive and memory impairment in the Barnes maze test. Additionally, PIL and VD presented higher levels of serum IFN-α1, but not IL-6. Mice supplemented with vit-D had reduced IgM and IgG deposits and increased pVDR expression in the brain after 180 days. The AUC-ROC curve demonstrated high sensitivity and specificity for IgM and IgG in the brain. We observed neuropsychiatric manifestations in this model of systemic lupus erythematosus (SLE), strongly corroborating to PIL model being suitable as a neuropsychiatric lupus (NPSLE) model. Vit-D was able to reduce immunoglobulin deposits in the brain and influenced the levels of serum IL-6 in the animals assessed. Also, it improved memory, but it had no effect on depressive and anxious-like behavior.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased expression of CXCL10 and CCL3 salivary gland chemokines in primary Sjögren's syndrome detected and systematically quantified using novel RNAscope® in situ hybridisation. 利用新型 RNAscope® 原位杂交技术检测并系统量化原发性斯约格伦综合征患者唾液腺趋化因子 CXCL10 和 CCL3 的表达。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-10-22 DOI: 10.1093/cei/uxae087

Hanne Borge, Ingrid Beate Ringstad, Lara A Aqrawi, Siren Fromreide, Harsh Nitin Dongre, Hilde Kanli Galtung, Janicke Liaaen Jensen, Kathrine Skarstein

{"title":"Increased expression of CXCL10 and CCL3 salivary gland chemokines in primary Sjögren's syndrome detected and systematically quantified using novel RNAscope® in situ hybridisation.","authors":"Hanne Borge, Ingrid Beate Ringstad, Lara A Aqrawi, Siren Fromreide, Harsh Nitin Dongre, Hilde Kanli Galtung, Janicke Liaaen Jensen, Kathrine Skarstein","doi":"10.1093/cei/uxae087","DOIUrl":"https://doi.org/10.1093/cei/uxae087","url":null,"abstract":"Primary Sjogren's syndrome is a chronic inflammatory disease characterised by the destruction of exocrine glands. We have previously shown significantly upregulated levels of CXCL10 and CCL3 chemokines in saliva from Sjogren's syndrome patients. In this study, we examined the expression pattern and localisation of these chemokines at the site of inflammation in patients' minor salivary glands using novel RNAscope® in situ hybridisation. Minor salivary glands from 33 primary Sjogren's syndrome patients and 22 non-Sjogren's syndrome sicca controls were included. The biopsies were formalin- fixed, paraffin-embedded and histopathologically evaluated. The CXCL10 and CCL3 mRNA expression in the glandular tissue was investigated using reverse transcription quantitative real-time polymerase chain reaction followed by RNAscope® in situ hybridisation. The mRNA expression of CXCL10 was higher than CCL3 in all patients. Significantly elevated expression of CXCL10 and CCL3 was detected in patients that also expressed autoantibody positivity and a positive biopsy for mononuclear cell infiltrates when compared to controls. CXCL10 was localised as clusters within focal infiltrates as well as adjacent to acinar and ductal epithelium, while CCL3 was expressed as scattered single mRNA molecules in focal infiltrates and in acinar cells. Our findings suggest CXCL10 as a possible disease biomarker in primary Sjogren's syndrome due to its upregulated expression in both saliva and minor salivary glands of patients and the localisation in the tissue. This should be re-assessed in a larger primary Sjogren's syndrome patient cohort, followed by additional functional studies to further validate its potential as a disease biomarker.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Accuracy of Saliva-based Testing as a Vibrio Cholerae Surveillance Tool among naturally-infected patients. 将唾液检测作为自然感染患者霍乱弧菌监测工具的诊断准确性。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-10-21 DOI: 10.1093/cei/uxae092

Caroline C Chisenga, Bernard Phiri, Harriet Ng'ombe, Mutinta Muchimba, Fraser Liswaniso, Biana Bernshtein, Adam F Cunningham, David Sack, Samuel Bosomprah

{"title":"Diagnostic Accuracy of Saliva-based Testing as a Vibrio Cholerae Surveillance Tool among naturally-infected patients.","authors":"Caroline C Chisenga, Bernard Phiri, Harriet Ng'ombe, Mutinta Muchimba, Fraser Liswaniso, Biana Bernshtein, Adam F Cunningham, David Sack, Samuel Bosomprah","doi":"10.1093/cei/uxae092","DOIUrl":"10.1093/cei/uxae092","url":null,"abstract":"Saliva, as a diagnostic medium, offers a promising alternative to blood by virtue of its non-invasive collection, which enhances patient compliance, especially in paediatric and geriatric populations. In this study, we assessed the utility of saliva as a non-invasive medium for measuring V. cholerae-specific serum antibodies in naturally infected individuals. We tested paired serum and saliva samples obtained from a total of 63 cholera patients enrolled in a cohort study. Vibriocidal antibodies assay (IgM/IgG) as markers for accurate determination was used to determine cholera specific antibody levels. Using receiver operating characteristics (ROC) curve, we found that the best cut-off that maximizes (sensitivity + specificity) is 10 titres. At this saliva titre, the sensitivity is 76.9% (95%CI: 60.9%, 87.7%) and specificity is 80.0% (95%CI: 56.6%, 92.5%). Using Spearman's correlation coefficient, we also found evidence of a positive correlation between Vibrio Cholerae saliva and serum antibodies (rho=0.66, p<0.001). In conclusion, saliva-based diagnostic cholera tests has high diagnostic accuracy, and would be advantageous, cheaper, and quicker for early diagnosis of severe cholera outcomes.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD8 cell-derived granzyme B may be a predictor for Coronary artery involvement and MACE in Takayasu arteritis patients. CD8细胞衍生的颗粒酶B可能是高安动脉炎患者冠状动脉受累和MACE的预测因子。

IF 3.4 3区医学

Clinical and experimental immunology Pub Date : 2024-10-21 DOI: 10.1093/cei/uxae095

Taotao Li, Na Gao, Juan Du, Limin Zhao, Shiyu Yang, Yaxin Zhang, Junming Zhu, Haiou Hu, Zhiyu Qiao, Wei Cui, Lili Pan

{"title":"CD8 cell-derived granzyme B may be a predictor for Coronary artery involvement and MACE in Takayasu arteritis patients.","authors":"Taotao Li, Na Gao, Juan Du, Limin Zhao, Shiyu Yang, Yaxin Zhang, Junming Zhu, Haiou Hu, Zhiyu Qiao, Wei Cui, Lili Pan","doi":"10.1093/cei/uxae095","DOIUrl":"https://doi.org/10.1093/cei/uxae095","url":null,"abstract":"Coronary artery involvement (CAI) is a special but not rare manifestation of Takayasu arteritis (TAK). Granzyme B (GzmB) is a multifunctional protease associated with the immune system and coronary artery disease. However, its role in patients with TAK and CAI remains unclear. This study investigates the role of GzmB+ cell subsets in TAK. The study included 105 TAK patients and 58 healthy controls. The percentages of different GzmB+ cells in blood samples were analyzed by flow cytometry. We found that age, age at onset, BMI, disease duration month, hypertension, and hyperlipidemia were significantly different between TAK patients with and without CAI (P=0.000, P=0.038, P=0.003, P=0.031, P=0.039, P=0.000). The proportions of CD3+CD8+cells (P=0.001) and CD3+CD4+cells (P=0.000) in GzmB+ cells were significantly increased, while the proportion of CD3-CD56+cells (P=0.001) in GzmB+ cells was decreased in TAK patients. The proportions of three types of GzmB+ subsets in lymphocytes (CD3+CD4+GzmB+, CD3+CD8+GzmB+, CD3+CD56+ GzmB+) were higher in TAK patients with CAI compared to those without CAI (P=0.021, P=0.007, P=0.007). The increased proportion of CD3+CD8+GzmB+cells/lymphocytes was an independent risk factor for coronary involvement in TAK (OR=4.990 [1.766-14.098], P=0.002). Additionally, patients with a high CD3+CD8+GzmB+cells/lymphocytes ratio had a higher MACE rate than those with a low ratio in TAK (P=0.019). Our results indicate that CD8 cell-derived Gzm B may be a predictor for CAI and MACE in TAK patients. Targeting CD3+CD8+GzmB+ lymphocytes or using GzmB inhibitors could be a potential therapeutic approach for the treatment of CAI in TAK.","PeriodicalId":10268,"journal":{"name":"Clinical and experimental immunology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0