Chromosoma最新文献_第7页

BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris. BAC-和oligo-FISH图谱揭示了角菖蒲、蹄菖蒲和菜豆的染色体进化。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-09-01 Epub Date: 2021-04-28 DOI: 10.1007/s00412-021-00758-9

Lívia do Vale Martins, Fernanda de Oliveira Bustamante, Ana Rafaela da Silva Oliveira, Antônio Félix da Costa, Lidiane de Lima Feitoza, Qihua Liang, Hainan Zhao, Ana Maria Benko-Iseppon, María Muñoz-Amatriaín, Andrea Pedrosa-Harand, Jiming Jiang, Ana Christina Brasileiro-Vidal

{"title":"BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris.","authors":"Lívia do Vale Martins, Fernanda de Oliveira Bustamante, Ana Rafaela da Silva Oliveira, Antônio Félix da Costa, Lidiane de Lima Feitoza, Qihua Liang, Hainan Zhao, Ana Maria Benko-Iseppon, María Muñoz-Amatriaín, Andrea Pedrosa-Harand, Jiming Jiang, Ana Christina Brasileiro-Vidal","doi":"10.1007/s00412-021-00758-9","DOIUrl":"https://doi.org/10.1007/s00412-021-00758-9","url":null,"abstract":"Cytogenomic resources have accelerated synteny and chromosome evolution studies in plant species, including legumes. Here, we established the first cytogenetic map of V. angularis (Va, subgenus Ceratotropis) and compared this new map with those of V. unguiculata (Vu, subgenus Vigna) and P. vulgaris (Pv) by BAC-FISH and oligopainting approaches. We mapped 19 Vu BACs and 35S rDNA probes to the 11 chromosome pairs of Va, Vu, and Pv. Vigna angularis shared a high degree of macrosynteny with Vu and Pv, with five conserved syntenic chromosomes. Additionally, we developed two oligo probes (Pv2 and Pv3) used to paint Vigna orthologous chromosomes. We confirmed two reciprocal translocations (chromosomes 2 and 3 and 1 and 8) that have occurred after the Vigna and Phaseolus divergence (~9.7 Mya). Besides, two inversions (2 and 4) and one translocation (1 and 5) have occurred after Vigna and Ceratotropis subgenera separation (~3.6 Mya). We also observed distinct oligopainting patterns for chromosomes 2 and 3 of Vigna species. Both Vigna species shared similar major rearrangements compared to Pv: one translocation (2 and 3) and one inversion (chromosome 3). The sequence synteny identified additional inversions and/or intrachromosomal translocations involving pericentromeric regions of both orthologous chromosomes. We propose chromosomes 2 and 3 as hotspots for chromosomal rearrangements and de novo centromere formation within and between Vigna and Phaseolus. Our BAC- and oligo-FISH mapping contributed to physically trace the chromosome evolution of Vigna and Phaseolus and its application in further studies of both genera.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 2-3","pages":"133-147"},"PeriodicalIF":1.6,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-021-00758-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38916377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Deletion of the XIST promoter from the human inactive X chromosome compromises polycomb heterochromatin maintenance. 从人类无活性X染色体上删除XIST启动子会损害多梳异染色质的维持。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-09-01 Epub Date: 2021-03-21 DOI: 10.1007/s00412-021-00754-z

Natalia Westervelt, Andrea Yoest, Sadia Sayed, Marina Von Zimmerman, Kelly Kaps, Brian P Chadwick

{"title":"Deletion of the XIST promoter from the human inactive X chromosome compromises polycomb heterochromatin maintenance.","authors":"Natalia Westervelt, Andrea Yoest, Sadia Sayed, Marina Von Zimmerman, Kelly Kaps, Brian P Chadwick","doi":"10.1007/s00412-021-00754-z","DOIUrl":"https://doi.org/10.1007/s00412-021-00754-z","url":null,"abstract":"Silencing most gene expression from all but one X chromosome in female mammals provides a means to overcome X-linked gene expression imbalances with males. Central to establishing gene silencing on the inactivated X chromosome are the actions of the long non-coding RNA XIST that triggers the repackaging of the chosen X into facultative heterochromatin. While understanding the mechanisms through which XIST expression is regulated and mediates its affects has been a major focus of research since its discovery, less is known about the role XIST plays in maintaining chromatin at the human inactive X chromosome (Xi). Here, we use genome engineering to delete the promoter of XIST to knockout expression from the Xi in non-cancerous diploid human somatic cells. Although some heterochromatin features exhibit limited change at the Xi, two of those assessed showed significant reductions including histone H2A monoubiquitylation at lysine 119 and histone H3 trimethylation at lysine 27, both of which are covalent histone modifications catalyzed by the polycomb repressive complexes 1 and 2 respectively. Coupled with these reductions, we observed an occasional gain of euchromatin signatures on Xp, but despite these signs of chromatin instability, we did not observe appreciable changes in the reactivation of genes from the Xi. Collectively, these data are consistent with maintenance of dosage compensation at the Xi involving multiple redundant layers of gene silencing.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 2-3","pages":"177-197"},"PeriodicalIF":1.6,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-021-00754-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25509164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Linear elements are stable structures along the chromosome axis in fission yeast meiosis. 在分裂酵母减数分裂中，线形因子是沿染色体轴的稳定结构。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-09-01 Epub Date: 2021-04-07 DOI: 10.1007/s00412-021-00757-w

Da-Qiao Ding, Atsushi Matsuda, Kasumi Okamasa, Yasushi Hiraoka

{"title":"Linear elements are stable structures along the chromosome axis in fission yeast meiosis.","authors":"Da-Qiao Ding, Atsushi Matsuda, Kasumi Okamasa, Yasushi Hiraoka","doi":"10.1007/s00412-021-00757-w","DOIUrl":"https://doi.org/10.1007/s00412-021-00757-w","url":null,"abstract":"The structure of chromosomes dramatically changes upon entering meiosis to ensure the successful progression of meiosis-specific events. During this process, a multilayer proteinaceous structure called a synaptonemal complex (SC) is formed in many eukaryotes. However, in the fission yeast Schizosaccharomyces pombe, linear elements (LinEs), which are structures related to axial elements of the SC, form on the meiotic cohesin-based chromosome axis. The structure of LinEs has been observed using silver-stained electron micrographs or in immunofluorescence-stained spread nuclei. However, the fine structure of LinEs and their dynamics in intact living cells remain to be elucidated. In this study, we performed live cell imaging with wide-field fluorescence microscopy as well as 3D structured illumination microscopy (3D-SIM) of the core components of LinEs (Rec10, Rec25, Rec27, Mug20) and a linE-binding protein Hop1. We found that LinEs form along the chromosome axis and elongate during meiotic prophase. 3D-SIM microscopy revealed that Rec10 localized to meiotic chromosomes in the absence of other LinE proteins, but shaped into LinEs only in the presence of all three other components, the Rec25, Rec27, and Mug20. Elongation of LinEs was impaired in double-strand break-defective rec12- cells. The structure of LinEs persisted after treatment with 1,6-hexanediol and showed slow fluorescence recovery from photobleaching. These results indicate that LinEs are stable structures resembling axial elements of the SC.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 2-3","pages":"149-162"},"PeriodicalIF":1.6,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-021-00757-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25567176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Chromosome Y pericentric heterochromatin is a primary target of HSF1 in male cells. Y染色体外中心异染色质是HSF1在男性细胞中的主要靶点。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2021-02-06 DOI: 10.1007/s00412-021-00751-2

Jessica Penin, Solenne Dufour, Virginie Faure, Sabrina Fritah, Daphné Seigneurin-Berny, Edwige Col, André Verdel, Claire Vourc'h

{"title":"Chromosome Y pericentric heterochromatin is a primary target of HSF1 in male cells.","authors":"Jessica Penin, Solenne Dufour, Virginie Faure, Sabrina Fritah, Daphné Seigneurin-Berny, Edwige Col, André Verdel, Claire Vourc'h","doi":"10.1007/s00412-021-00751-2","DOIUrl":"https://doi.org/10.1007/s00412-021-00751-2","url":null,"abstract":"The heat shock factor 1 (HSF1)-dependent transcriptional activation of human pericentric heterochromatin in heat-shocked cells is the most striking example of transcriptional activation of heterochromatin. Until now, pericentric heterochromatin of chromosome 9 has been identified as the primary target of HSF1, in both normal and tumor heat-shocked cells. Transcriptional awakening of this large genomic region results in the nuclear accumulation of satellite III (SATIII) noncoding RNAs (ncRNAs) and the formation in cis of specific structures known as nuclear stress bodies (nSBs). Here, we show that, in four different male cell lines, including primary human fibroblasts and amniocytes, pericentric heterochromatin of chromosome Y can also serve as a unique primary site of HSF1-dependent heterochromatin transcriptional activation, production of SATIII ncRNA, and nucleation of nuclear stress bodies (nSBs) upon heat shock. Our observation suggests that the chromosomal origin of SATIII transcripts in cells submitted to heat shock is not a determinant factor as such, but that transcription of SATIII repetitive units or the SATIII ncRNA molecules is the critical element of HSF1-dependent transcription activation of constitutive heterochromatin.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 1","pages":"53-60"},"PeriodicalIF":1.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-021-00751-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25337168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Imaging assay to probe the role of telomere length shortening on telomere-gene interactions in single cells. 成像分析，以探测端粒长度缩短在单细胞中端粒-基因相互作用中的作用。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2021-02-08 DOI: 10.1007/s00412-020-00747-4

Ning Zhang, Yanhui Li, Tsung-Po Lai, Jerry W Shay, Gaudenz Danuser

{"title":"Imaging assay to probe the role of telomere length shortening on telomere-gene interactions in single cells.","authors":"Ning Zhang, Yanhui Li, Tsung-Po Lai, Jerry W Shay, Gaudenz Danuser","doi":"10.1007/s00412-020-00747-4","DOIUrl":"10.1007/s00412-020-00747-4","url":null,"abstract":"Telomeres are repetitive non-coding nucleotide sequences (TTAGGGn) capping the ends of chromosomes. Progressive telomere shortening with increasing age has been associated with shifts in gene expression through models such as the telomere position effect (TPE), which suggests reduced interference of the telomere with transcriptional activity of increasingly more distant genes. A modification of the TPE model, referred to as Telomere Position Effects over Long Distance (TPE-OLD), explains why some genes 1-10 MB from a telomere are still affected by TPE, but genes closer to the telomere are not. Here, we describe an imaging approach to systematically examine the occurrence of TPE-OLD at the single cell level. Compared to existing methods, the pipeline allows rapid analysis of hundreds to thousands of cells, which is necessary to establish TPE-OLD as an acceptable mechanism of gene expression regulation. We examined two human genes, ISG15 and TERT, for which TPE-OLD has been described before. For both genes, we found less interaction with the telomere on the same chromosome in old cells compared to young cells; and experimentally elongated telomeres in old cells rescued the level of telomere interaction for both genes. However, the dependency of the interactions on the age progression from young to old cells varied. One model for the differences between ISG15 and TERT may relate to the markedly distinct interstitial telomeric sequence arrangement in the two genes. Overall, this provides a strong rationale for the role of telomere length shortening in the regulation of gene expression.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 1","pages":"61-73"},"PeriodicalIF":1.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25346099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects. 同中心 HSATII RNA 的异位表达会导致核 RNA 积累、MeCP2 募集和细胞分裂缺陷。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2021-02-13 DOI: 10.1007/s00412-021-00753-0

Catherine C Landers, Christina A Rabeler, Emily K Ferrari, Lia R D'Alessandro, Diana D Kang, Jessica Malisa, Safia M Bashir, Dawn M Carone

{"title":"Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects.","authors":"Catherine C Landers, Christina A Rabeler, Emily K Ferrari, Lia R D'Alessandro, Diana D Kang, Jessica Malisa, Safia M Bashir, Dawn M Carone","doi":"10.1007/s00412-021-00753-0","DOIUrl":"10.1007/s00412-021-00753-0","url":null,"abstract":"Within the pericentric regions of human chromosomes reside large arrays of tandemly repeated satellite sequences. Expression of the human pericentric satellite HSATII is prevented by extensive heterochromatin silencing in normal cells, yet in many cancer cells, HSATII RNA is aberrantly expressed and accumulates in large nuclear foci in cis. Expression and aggregation of HSATII RNA in cancer cells is concomitant with recruitment of key chromatin regulatory proteins including methyl-CpG binding protein 2 (MeCP2). While HSATII expression has been observed in a wide variety of cancer cell lines and tissues, the effect of its expression is unknown. We tested the effect of stable expression of HSATII RNA within cells that do not normally express HSATII. Ectopic HSATII expression in HeLa and primary fibroblast cells leads to focal accumulation of HSATII RNA in cis and triggers the accumulation of MeCP2 onto nuclear HSATII RNA bodies. Further, long-term expression of HSATII RNA leads to cell division defects including lagging chromosomes, chromatin bridges, and other chromatin defects. Thus, expression of HSATII RNA in normal cells phenocopies its nuclear accumulation in cancer cells and allows for the characterization of the cellular events triggered by aberrant expression of pericentric satellite RNA.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 1","pages":"75-90"},"PeriodicalIF":1.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25372872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

spn-A/rad51 mutant exhibits enhanced genomic damage, cell death and low temperature sensitivity in somatic tissues. spn-A/rad51突变体在体细胞组织中表现出增强的基因组损伤、细胞死亡和低温敏感性。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2020-11-22 DOI: 10.1007/s00412-020-00746-5

Chaitali Khan, Sonia Muliyil, Champakali Ayyub, B J Rao

{"title":"spn-A/rad51 mutant exhibits enhanced genomic damage, cell death and low temperature sensitivity in somatic tissues.","authors":"Chaitali Khan, Sonia Muliyil, Champakali Ayyub, B J Rao","doi":"10.1007/s00412-020-00746-5","DOIUrl":"https://doi.org/10.1007/s00412-020-00746-5","url":null,"abstract":"Homologous recombination (HR) is one of the key pathways to repair double-strand breaks (DSBs). Rad51 serves an important function of catalysing strand exchange between two homologous sequences in the HR pathway. In higher organisms, rad51 function is indispensable with its absence leading to early embryonic lethality, thus precluding any mechanistic probing of the system. In contrast, the absence of Drosophila rad51 (spn-A/rad51) has been associated with defects in the germline, without any reported detrimental consequences to Drosophila somatic tissues. In this study, we have performed a systematic analysis of developmental defects in somatic tissues of spn-A mutant flies by using genetic complementation between multiple spn-A alleles. Our current study, for the first time, uncovers a requirement for spn-A in somatic tissue maintenance during both larval and pupal stages. Also, we show that spn-A mutant exhibits patterning defects in abdominal cuticle in the stripes and bristles, while there appear to be only subtle defects in the adult wing and eye. Interestingly, spn-A mutant shows a discernible phenotype of low temperature sensitivity, suggesting a role of spn-A in temperature sensitive cellular processes. In summary, our study describes the important role played by spn-A/rad51 in Drosophila somatic tissues.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 1","pages":"3-14"},"PeriodicalIF":1.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-020-00746-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38632529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Julio S. Rufas: A true chromosome lover. 胡里奥·s·鲁法斯:一个真正的染色体爱好者。

IF 1.6 4区生物学

Chromosoma Pub Date : 2021-03-01 DOI: 10.1007/s00412-020-00748-3

Carlos García de la Vega, Rocío Gómez, Jesús Page, María Teresa Parra, Juan Luis Santos, José A Suja, Alberto Viera

引用次数: 0

A deformation energy model reveals sequence-dependent property of nucleosome positioning. 变形能模型揭示了核小体定位的序列依赖特性。

IF 2.5 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2021-01-16 DOI: 10.1007/s00412-020-00750-9

Guoqing Liu, Hongyu Zhao, Hu Meng, Yongqiang Xing, Lu Cai

引用次数: 0

Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds. 当前寡聚交叉荧光显微镜探针设计的局限性，以浮萍的染色体进化研究为例。

IF 2.5 4区生物学

Chromosoma Pub Date : 2021-03-01 Epub Date: 2021-01-14 DOI: 10.1007/s00412-020-00749-2

Phuong T N Hoang, Jean-Marie Rouillard, Jiří Macas, Ivona Kubalová, Veit Schubert, Ingo Schubert

{"title":"Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds.","authors":"Phuong T N Hoang, Jean-Marie Rouillard, Jiří Macas, Ivona Kubalová, Veit Schubert, Ingo Schubert","doi":"10.1007/s00412-020-00749-2","DOIUrl":"10.1007/s00412-020-00749-2","url":null,"abstract":"Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a). Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of oligo-probes to elucidate chromosome evolution across duckweed genera.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"130 1","pages":"15-25"},"PeriodicalIF":2.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38819323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0