The Plant Pathology Journal最新文献_第9页

Methylome Analysis of Two Xanthomonas spp. Using Single-Molecule Real-Time Sequencing 两种黄单胞菌单分子实时测序的甲基组分析

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.FT.10.2016.0216

H. Seong, Hye-Jee Park, Eunji Hong, Sung Chul Lee, W. Sul, Sang-Wook Han

{"title":"Methylome Analysis of Two Xanthomonas spp. Using Single-Molecule Real-Time Sequencing","authors":"H. Seong, Hye-Jee Park, Eunji Hong, Sung Chul Lee, W. Sul, Sang-Wook Han","doi":"10.5423/PPJ.FT.10.2016.0216","DOIUrl":"https://doi.org/10.5423/PPJ.FT.10.2016.0216","url":null,"abstract":"Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been documented. Here, we report the methylomes of Xanthomonas axonopodis pv. glycines (Xag) strain 8ra and X. campestris pv. vesicatoria (Xcv) strain 85-10. We identified N6-methyladenine (6mA) and N4-methylcytosine (4mC) modification in both genomes. In addition, we assigned putative DNA methylation motifs including previously unreported methylation motifs via REBASE and MotifMaker, and compared methylation patterns in both species. Although Xag and Xcv belong to the same genus, their methylation patterns were dramatically different. The number of 4mC DNA bases in Xag (66,682) was significantly higher (29 fold) than in Xcv (2,321). In contrast, the number of 6mA DNA bases (4,147) in Xag was comparable to the number in Xcv (5,491). Strikingly, there were no common or shared motifs in the 10 most frequently methylated motifs of both strains, indicating they possess unique species- or strain-specific methylation motifs. Among the 20 most frequent motifs from both strains, for 9 motifs at least 1% of the methylated bases were located in putative promoter regions. Methylome analysis by SMRT sequencing technology is the first step toward understanding the biology and functions of DNA methylation in this genus.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114605523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri 紫斑病病原菌Alternaria porri (Ellis) Ciferri的新抗性来源鉴定及基因型差异反应

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.02.2016.0034

S. Nanda, S. Chand, P. Mandal, P. Tripathy, R. Joshi

{"title":"Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri","authors":"S. Nanda, S. Chand, P. Mandal, P. Tripathy, R. Joshi","doi":"10.5423/PPJ.OA.02.2016.0034","DOIUrl":"https://doi.org/10.5423/PPJ.OA.02.2016.0034","url":null,"abstract":"Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession ‘CBT-Ac77’ and cultivar ‘Arka Kalyan’ were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A. porri, revealed that 7 days after inoculation was suitable to observe the disease severity. In vitro screening of 43 genotypes for resistance to A. porri revealed two resistant lines. An additional 14 genotypes showed consistent moderate resistance in the field as well as in vitro evaluations. Among the related Allium species, A. schoenoprasum and A. roylei showed the least disease index and can be used for interspecific hybridization with cultivated onion. Differential reaction analysis of three A. porri isolates (Apo-Chiplima, Apn-Nasik, Apg-Guntur) in 43 genotypes revealed significant variation among the evaluated Allium species (P = 0.001). All together, the present study suggest that, the newly identified resistance sources can be used as potential donors for ongoing purple blotch resistance breeding program in India.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"323 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132508168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium 一种抗真菌细菌噬菌体的分离与鉴定

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.NT.07.2016.0153

Aryan Rahimi-Midani, Kyoung-Ho Kim, Seon-Woo Lee, S. Jung, T. Choi

引用次数: 1

Outbreak and Spread of Bacterial Canker of Kiwifruit Caused by Pseudomonas syringae pv. actinidiae Biovar 3 in Korea 丁香假单胞菌引起的猕猴桃细菌性溃疡病的爆发与传播。韩国的actitindiae Biovar 3

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.05.2016.0122

Gyoung-Hee Kim, Kwang-Hyung Kim, Kyeong In Son, E. Choi, Young Sun Lee, J. Jung, Y. Koh

{"title":"Outbreak and Spread of Bacterial Canker of Kiwifruit Caused by Pseudomonas syringae pv. actinidiae Biovar 3 in Korea","authors":"Gyoung-Hee Kim, Kwang-Hyung Kim, Kyeong In Son, E. Choi, Young Sun Lee, J. Jung, Y. Koh","doi":"10.5423/PPJ.OA.05.2016.0122","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2016.0122","url":null,"abstract":"A bacterial pathogen, Pseudomonas syringae pv. actinidiae (Psa), is a causal agent of kiwifruit bacterial canker worldwide. Psa biovar 3 (Psa3) was first detected in 2011 at an orchard in Dodeok-myeon, Goheunggun, Jeonnam Province in Korea. In this study, we present the results of an epidemiological study regarding Psa3 occurrence on kiwifruit orchards in Korea for the period of 2013 to 2015. Since the first detection of Psa3 in 2011, there was no further case reported by 2013. However, Psa3 was rapidly spreading to 33 orchards in 2014; except for three orchards in Sacheonsi, Gyeongnam Province, most cases were reported in Jeju Island. Entering 2015, bacterial canker by Psa3 became a pandemic in Korea, spreading to 72 orchards in Jeju Island, Jeonnam, and Gyeongnam Provinces. Our epidemiological study indicated that the first Psa3 incidence in 2011 might result from an introduction of Psa3 through imported seedlings from China in 2006. Apart from this, it was estimated that most Psa3 outbreaks from 2014 to 2015 were caused by pollens imported from New Zealand and China for artificial pollination. Most kiwifruit cultivars growing in Korea were infected with Psa3; yellow-fleshed cultivars (Yellow-king, Hort16A, Enza-gold, Zecy-gold, and Haegeum), red-fleshed cultivars (Hongyang and Enza-Red), green-fleshed cultivars (Hayward and Daeheung), and even a kiwiberry (Skinny-green). However, susceptibility to canker differed among cultivars; yellow- and red-fleshed cultivars showed much more severe symptoms compared to the green-fleshed cultivars of kiwifruit and a kiwiberry.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116829502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis 葡萄农杆菌感染葡萄节间组织中疾病相关代谢物的分析

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.FT.08.2016.0163

S. Jung, Y. Hur, J. Preece, O. Fiehn, Young-Ho Kim

{"title":"Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis","authors":"S. Jung, Y. Hur, J. Preece, O. Fiehn, Young-Ho Kim","doi":"10.5423/PPJ.FT.08.2016.0163","DOIUrl":"https://doi.org/10.5423/PPJ.FT.08.2016.0163","url":null,"abstract":"Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses, respectively. In this, 4, 4, and 2 Vitis species were classified into RR, SR, and SS, respectively. Gas chromatography mass spectrometry (GC-MS) analysis of the grapevine stem metabolites with A. vitis infection showed 134 metabolites in various compound classes critically occurred, which were differentially clustered with the response types by the principal component analysis. Multivariate analysis of the metabolite profile revealed that 11 metabolites increased significantly in relation to the response types, mostly at post-inoculation stages, more prevalently (8 metabolites) at two days after inoculation than other stages, and more related to SS (7 metabolites) than RR (3 metabolites) or SR (one metabolite). This suggests most of the disease-related metabolites may be rarely pre-existing but mostly induced by pathogen infection largely for facilitating gall development except stilbene compound resveratrol, a phytoalexin that may be involved in the resistance response. All of these aspects may be used for the selection of resistant grapevine cultivars and their rootstocks for the control of the crown gall disease of the grapevine.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114187196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.NT.09.2015.0197

A. Mukherjee, P. Mukherjee, S. Kranthi

引用次数: 11

Effectiveness of Different Classes of Fungicides on Botrytis cinerea Causing Gray Mold on Fruit and Vegetables 不同种类杀菌剂对果蔬灰霉病灰霉病的防治效果

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.NT.05.2016.0114

Joon-Oh Kim, Jong-Hwan Shin, Adiyantara Gumilang, Keun Chung, K. Choi, Kyoung-Su Kim

引用次数: 19

Xanthomonas euvesicatoria Causes Bacterial Spot Disease on Pepper Plant in Korea 韩国辣椒黄单胞菌引起细菌性斑疹病

The Plant Pathology Journal Pub Date : 2016-10-01 DOI: 10.5423/PPJ.OA.01.2016.0016

Min-Seong Kyeon, Soo-Hyeong Son, Young-Hee Noh, Yong-Eon Kim, Hyok-In Lee, J. Cha

{"title":"Xanthomonas euvesicatoria Causes Bacterial Spot Disease on Pepper Plant in Korea","authors":"Min-Seong Kyeon, Soo-Hyeong Son, Young-Hee Noh, Yong-Eon Kim, Hyok-In Lee, J. Cha","doi":"10.5423/PPJ.OA.01.2016.0016","DOIUrl":"https://doi.org/10.5423/PPJ.OA.01.2016.0016","url":null,"abstract":"In 2004, bacterial spot-causing xanthomonads (BSX) were reclassified into 4 species—Xanthomonas euvesicatoria, X. vesicatoria, X. perforans, and X. gardneri. Bacterial spot disease on pepper plant in Korea is known to be caused by both X. axonopodis pv. vesicatoria and X. vesicatoria. Here, we reidentified the pathogen causing bacterial spots on pepper plant based on the new classification. Accordingly, 72 pathogenic isolates were obtained from the lesions on pepper plants at 42 different locations. All isolates were negative for pectolytic activity. Five isolates were positive for amylolytic activity. All of the Korean pepper isolates had a 32 kDa-protein unique to X. euvesicatoria and had the same band pattern of the rpoB gene as that of X. euvesicatoria and X. perforans as indicated by PCR-restriction fragment length polymorphism analysis. A phylogenetic tree of 16S rDNA sequences showed that all of the Korean pepper plant isolates fit into the same group as did all the reference strains of X. euvesicatoria and X. perforans. A phylogenetic tree of the nucleotide sequences of 3 housekeeping genes—gapA, gyrB, and lepA showed that all of the Korean pepper plant isolates fit into the same group as did all of the references strains of X. euvesicatoria. Based on the phenotypic and genotypic characteristics, we identified the pathogen as X. euvesicatoria. Neither X. vesicatoria, the known pathogen of pepper bacterial spot, nor X. perforans, the known pathogen of tomato plant, was isolated. Thus, we suggest that the pathogen causing bacterial spot disease of pepper plants in Korea is X. euvesicatoria.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132747466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Comparative Analyses of Tomato yellow leaf curl virus C4 Protein-Interacting Host Proteins in Healthy and Infected Tomato Tissues 番茄黄曲叶病毒C4蛋白与侵染番茄组织相互作用宿主蛋白的比较分析

The Plant Pathology Journal Pub Date : 2016-10-01 DOI: 10.5423/PPJ.FT.08.2016.0165

Namgyu Kim, Jinnyun Kim, Bongjun Bang, Inyoung Kim, Hyun-Hee Lee, Jungwook Park, Y. Seo

{"title":"Comparative Analyses of Tomato yellow leaf curl virus C4 Protein-Interacting Host Proteins in Healthy and Infected Tomato Tissues","authors":"Namgyu Kim, Jinnyun Kim, Bongjun Bang, Inyoung Kim, Hyun-Hee Lee, Jungwook Park, Y. Seo","doi":"10.5423/PPJ.FT.08.2016.0165","DOIUrl":"https://doi.org/10.5423/PPJ.FT.08.2016.0165","url":null,"abstract":"Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. TYLCV causes severe problems in sub-tropical and tropical countries, as well as in Korea. However, the mechanism of TYLCV infection remains unclear, although the function of each viral component has been identified. TYLCV C4 codes for a small protein involved in various cellular functions, including symptom determination, gene silencing, viral movement, and induction of the plant defense response. In this study, through yeast-two hybrid screenings, we identified TYLCV C4-interacting host proteins from both healthy and symptom-exhibiting tomato tissues, to determine the role of TYLCV C4 proteins in the infection processes. Comparative analyses of 28 proteins from healthy tissues and 36 from infected tissues showing interactions with TYLCV C4 indicated that TYLCV C4 mainly interacts with host proteins involved in translation, ubiquitination, and plant defense, and most interacting proteins differed between the two tissues but belong to similar molecular functional categories. Four proteins—two ribosomal proteins, S-adenosyl-L-homocysteine hydrolase, and 14-3-3 family protein—were detected in both tissues. Furthermore, the identified proteins in symptom-exhibiting tissues showed greater involvement in plant defenses. Some are key regulators, such as receptor-like kinases and pathogenesis-related proteins, of plant defenses. Thus, TYLCV C4 may contribute to the suppression of host defense during TYLCV infection and be involved in ubiquitination for viral infection.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127815986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli 大肠杆菌表达葡萄扇叶病毒运动蛋白多克隆抗体的制备

The Plant Pathology Journal Pub Date : 2016-10-01 DOI: 10.5423/PPJ.OA.01.2016.0031

D. Koolivand, N. S. Bashir, S. A. Behjatnia, R. Joozani

{"title":"Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli","authors":"D. Koolivand, N. S. Bashir, S. A. Behjatnia, R. Joozani","doi":"10.5423/PPJ.OA.01.2016.0031","DOIUrl":"https://doi.org/10.5423/PPJ.OA.01.2016.0031","url":null,"abstract":"The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting. The expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonal antibodies and application for the virus detection.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"71 s318","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113954174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12