The Plant Pathology Journal最新文献_第8页

Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker 抑制柑橘黄单胞菌的合成抗菌肽的筛选。导致柑橘溃疡病

The Plant Pathology Journal Pub Date : 2017-02-01 DOI: 10.5423/PPJ.NT.09.2015.0188

Jeahyuk Choi, E. Park, Seweon Lee, J. Hyun, K. Baek

{"title":"Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker","authors":"Jeahyuk Choi, E. Park, Seweon Lee, J. Hyun, K. Baek","doi":"10.5423/PPJ.NT.09.2015.0188","DOIUrl":"https://doi.org/10.5423/PPJ.NT.09.2015.0188","url":null,"abstract":"Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fourteen hexapeptides were selected by using positional scanning of synthetic peptide combinatorial libraries. Each hexapeptide showed different antimicrobial spectrum against Bacillus, Pseudomonas, Xanthomonas, and Candida species. Intriguingly, BHC10 showed bactericidal activity exclusively on Xanthomonas citri subsp. citri (Xcc), while BHC7 was none-active exclusively against two Pseudomonas spp. at concentration of 100 μg/ml suggesting potential selectivity constrained in hexapeptide frame. Three hexapeptides, BHC02, 06 and 11, showed bactericidal activities against various Xcc strains at concentration of 10 μg/ml. When they were co-infiltrated with pathogens into citrus leaves the disease progress was suppressed significantly. Further study would be needed to confirm the actual disease control capacity of the selected hexapeptides.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125597696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Morphogenetic Alterations of Alternaria alternata Exposed to Dicarboximide Fungicide, Iprodione 杀真菌剂异丙二酮对互花霉形态发生的影响

The Plant Pathology Journal Pub Date : 2017-02-01 DOI: 10.5423/PPJ.NT.06.2016.0145

Eunji Kim, H. Lee, Young Ho Kim

{"title":"Morphogenetic Alterations of Alternaria alternata Exposed to Dicarboximide Fungicide, Iprodione","authors":"Eunji Kim, H. Lee, Young Ho Kim","doi":"10.5423/PPJ.NT.06.2016.0145","DOIUrl":"https://doi.org/10.5423/PPJ.NT.06.2016.0145","url":null,"abstract":"Fungicide-resistant Alternaria alternata impede the practical control of the Alternaria diseases in crop fields. This study aimed to investigate cytological fungicide resistance mechanisms of A. alternata against dicarboximide fungicide iprodione. A. alternata isolated from cactus brown spot was cultured on potato-dextrose agar (PDA) with or without iprodione, and the fungal cultures with different growth characteristics from no, initial and full growth were observed by light and electron microscopy. Mycelia began to grow from one day after incubation (DAI) and continued to be in full growth (control-growth, Con-G) on PDA without fungicide, while on PDA with iprodione, no fungal growth (iprodione-no growth, Ipr-N) occurred for the first 3 DAI, but once the initial growth (iprodione-initial growth, Ipr-I) began at 4–5 DAI, the colonies grew and expanded continuously to be in full growth (iprodione-growth, Ipr-G), suggesting Ipr-I may be a turning moment of the morphogenetic changes resisting fungicidal toxicity. Con-G formed multicellular conidia with cell walls and septa and intact dense cytoplasm. In Ipr-N, fungal sporulation was inhibited by forming mostly undeveloped unicellular conidia with degraded and necrotic cytoplasm. However, in Ipr-I, conspicuous cellular changes occurred during sporulation by forming multicellular conidia with double layered (thickened) cell walls and accumulation of proliferated lipid bodies in the conidial cytoplasm, which may inhibit the penetration of the fungicide into conidial cells, reducing fungicide-associated toxicity, and may be utilized as energy and nutritional sources, respectively, for the further fungal growth to form mature colonies as in Ipr-G that formed multicellular conidia with cell walls and intact cytoplasm with lipid bodies as in Con-G.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"225 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130754362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization 利用PCR和微管杂交技术构建消除炭疽病真菌潜伏感染的草莓苗圃生产体系

The Plant Pathology Journal Pub Date : 2017-02-01 DOI: 10.5423/PPJ.NT.05.2016.0132

K. Furuta, S. Nagashima, T. Inukai, C. Masuta

引用次数: 5

Effects of Soil Textures on Infectivity of Root-Knot Nematodes on Carrot 土壤质地对胡萝卜根结线虫侵染力的影响

The Plant Pathology Journal Pub Date : 2017-02-01 DOI: 10.5423/PPJ.OA.07.2016.0155

Eunji Kim, Y. Seo, Yong Su Kim, Yong-Keun Park, Young Ho Kim

{"title":"Effects of Soil Textures on Infectivity of Root-Knot Nematodes on Carrot","authors":"Eunji Kim, Y. Seo, Yong Su Kim, Yong-Keun Park, Young Ho Kim","doi":"10.5423/PPJ.OA.07.2016.0155","DOIUrl":"https://doi.org/10.5423/PPJ.OA.07.2016.0155","url":null,"abstract":"This study was conducted to examine infectivity (penetration and gall and egg-mass formations) of the root-knot nematodes, Meloidogyne incognita and M. hapla, on carrots grown in soil conditions of 5 different soil textures consisting of bed-soil (b) and sand (s) mixtures (b-s mixtures) at the ratios of 10:0, 7:3, 5:5, 3:7, and 0:10. For M. incognita, the nematode penetration rates in b-s of 0:10 (100% sand) were significantly higher than in the other b-s mixtures, more greatly at 2 and 5 days after inoculation than at 10 DAI, while no significant differences in the penetration rates were mostly shown for M. hapla at the above DAI. However, for both nematodes, gall and egg-mass formations were remarkably increased in the b-s mixture of 0:10, compared to the other b-s mixtures, which is coincided with the general aspects of severe nematode infestations in sandy soils. This suggests the increased gall and egg-mass formations of M. incognita should be derived from the increased penetration rates in the sandy soil conditions, which provide a sufficient aeration due to coarse soil nature for the nematodes, leading to their mobility increased for the enhanced root penetration. For M. hapla, it is suggested that the sandy soil conditions affect positively on the healthy plant growth with little accumulation of the inhibitory materials and sufficient aeration, enhancing the nematode growth and feeding activities. All of these aspects provide information reliable for the development screening techniques efficient for the evaluation of the nematode resistance in the breeding programs.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127140885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction 多重聚合酶链反应同时检测水稻三种细菌性种传病害

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.NT.05.2016.0118

I. Kang, Mi-Hyung Kang, T. Noh, H. Shim, D. Shin, Su-Jin Heu

引用次数: 8

Digital Infrared Thermal Imaging of Crape Myrtle Leaves Infested with Sooty Mold 黑霉侵染紫薇叶片的数字红外热成像研究

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.NT.04.2016.0112

Jiyeon Kim, Si-gyun Kweon, Junhyung Park, Harim Lee, Ki Woo Kim

引用次数: 6

Altering Conidial Dispersal of Alternaria solani by Modifying Microclimate in Tomato Crop Canopy 改变番茄作物冠层小气候对茄斑孢孢子扩散的影响

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.06.2015.0101

P. Jambhulkar, N. Jambhulkar, M. Meghwal, G. S. Ameta

{"title":"Altering Conidial Dispersal of Alternaria solani by Modifying Microclimate in Tomato Crop Canopy","authors":"P. Jambhulkar, N. Jambhulkar, M. Meghwal, G. S. Ameta","doi":"10.5423/PPJ.OA.06.2015.0101","DOIUrl":"https://doi.org/10.5423/PPJ.OA.06.2015.0101","url":null,"abstract":"Early blight of tomato caused by Alternaria solani, is responsible for severe yield losses in tomato. The conidia survive on soil surface and old dry lower leaves of the plant and spread when suitable climatic conditions are available. Macroclimatic study reveals that highest inoculum concentration of Alternaria spores appeared in May 2012 to 2013 and lowest concentration during January 2012 to 2013. High night temperature positively correlated and significantly (P < 0.01) involved in conidial spore dispersal and low relative humidity (RH) displayed significant (P < 0.05) but negative correlation with conidial dispersal. The objective of the study was to modify microclimatic conditions of tomato crop canopy which may hamper conidial dispersal and reduce disease severity. We evaluated effect of marigold intercropping and plastic mulching singly and in consortia on A. solani conidial density, tomato leaf damage and microclimatic parameters as compar to tomato alone (T). Tomato-marigold intercropping–plastic mulching treatment (T + M + P) showed 35–39% reduction in disease intensity as compared to tomato alone. When intercropped with tomato, marigold served as barrier to conidial movement and plastic mulching prevented evapotranspiration and reduced the canopy RH that resulted in less germination of A. solani spores. Marigold intercropping and plastic mulching served successfully as physical barrier against conidial dissemination to diminish significantly the tomato foliar damage produced by A. solani.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116699638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

D-PSA-K: A Model for Estimating the Accumulated Potential Damage on Kiwifruit Canes Caused by Bacterial Canker during the Growing and Overwintering Seasons D-PSA-K:猕猴桃生长期和越冬期细菌溃疡病对猕猴桃植株累积潜在危害的估计模型

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.03.2016.0068

Ki Seok Do, B. Chung, J. Joa

{"title":"D-PSA-K: A Model for Estimating the Accumulated Potential Damage on Kiwifruit Canes Caused by Bacterial Canker during the Growing and Overwintering Seasons","authors":"Ki Seok Do, B. Chung, J. Joa","doi":"10.5423/PPJ.OA.03.2016.0068","DOIUrl":"https://doi.org/10.5423/PPJ.OA.03.2016.0068","url":null,"abstract":"We developed a model, termed D-PSA-K, to estimate the accumulated potential damage on kiwifruit canes caused by bacterial canker during the growing and overwintering seasons. The model consisted of three parts including estimation of the amount of necrotic lesion in a non-frozen environment, the rate of necrosis increase in a freezing environment during the overwintering season, and the amount of necrotic lesion on kiwifruit canes caused by bacterial canker during the overwintering and growing seasons. We evaluated the model’s accuracy by comparing the observed maximum disease incidence on kiwifruit canes against the damage estimated using weather and disease data collected at Wando during 1994–1997 and at Seogwipo during 2014–2015. For the Hayward cultivar, D-PSA-K estimated the accumulated damage as approximately nine times the observed maximum disease incidence. For the Hort16A cultivar, the accumulated damage estimated by D-PSA-K was high when the observed disease incidence was high. D-PSA-K could assist kiwifruit growers in selecting optimal sites for kiwifruit cultivation and establishing improved production plans by predicting the loss in kiwifruit production due to bacterial canker, using past weather or future climate change data.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131558348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice 致病相关蛋白10的过表达增强了水稻的生物和非生物胁迫耐受性

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.06.2016.0141

Jingni Wu, Sang Gon Kim, K. Kang, Ju-Gon Kim, Sangryeol Park, Ravi Gupta, Yong Hwan Kim, Yiming Wang, S. Kim

{"title":"Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice","authors":"Jingni Wu, Sang Gon Kim, K. Kang, Ju-Gon Kim, Sangryeol Park, Ravi Gupta, Yong Hwan Kim, Yiming Wang, S. Kim","doi":"10.5423/PPJ.OA.06.2016.0141","DOIUrl":"https://doi.org/10.5423/PPJ.OA.06.2016.0141","url":null,"abstract":"Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named “jasmonic acid inducible pathogenesis-related class 10” (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122116305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 69

Management of Powdery Mildew in Squash by Plant and Alga Extract Biopesticides 植物和藻类提取物生物农药防治南瓜白粉病的研究

The Plant Pathology Journal Pub Date : 2016-12-01 DOI: 10.5423/PPJ.OA.05.2016.0131

Shouan Zhang, Z. Mersha, G. Vallad, Cheng-Hua Huang

{"title":"Management of Powdery Mildew in Squash by Plant and Alga Extract Biopesticides","authors":"Shouan Zhang, Z. Mersha, G. Vallad, Cheng-Hua Huang","doi":"10.5423/PPJ.OA.05.2016.0131","DOIUrl":"https://doi.org/10.5423/PPJ.OA.05.2016.0131","url":null,"abstract":"Although many fungicides are registered for use to control powdery mildew on cucurbits, management of resistance to fungicides in pathogen populations still remains a major challenge. Two biopesticides Regalia SC and HMO 736 were evaluated in the greenhouse and field for their efficacy against powdery mildew in squash. In greenhouses, Regalia SC alone significantly (P < 0.05) reduced powdery mildew compared to the nontreated control, and was as effective as the chemical standard Procure 480SC (triflumizole). In alternation with Procure 480SC, Regalia SC demonstrated greater or equivalent effects on reducing the disease. HMO 736 alone showed varying levels of disease control, but alternating with Procure 480SC significantly improved control efficacy. In addition, application of Regalia SC or HMO 736 each in alternation with Procure 480SC significantly increased the chlorophyll content in leaves and the total fresh weight of squash plants, when compared with the water control, Regalia SC and HMO 736 alone. In field trials, application of Regalia SC and HMO 736 each alone significantly reduced disease severity in one of two field trials during the early stage of disease development, but not during later stages when disease pressure became high. Both Regalia SC and HMO 736 each applied in alternation with Procure 480SC significantly improved the control efficacy compared to Procure 480SC alone. Results from this study demonstrated that an integrated management program can be developed for powdery mildew in squash by integrating the biopesticides Regalia SC, HMO 736 with the chemical fungicide Procure 480SC.","PeriodicalId":101515,"journal":{"name":"The Plant Pathology Journal","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129721465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19