Tropical biomedicine最新文献_第6页

Development and validation of a PCR-based method to differentiate human sex in blood-fed Aedes aegypti (Diptera: Culicidae). 开发和验证基于 PCR 的方法，以区分血饲埃及伊蚊（双翅目：Culicidae）的人类性别。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.012

L Rathod, S Mishra, S Samuel, K Yadav, G Sharma, S Singh, M Kumar, R R Tiwari, S Shubham, D K Sarma

{"title":"Development and validation of a PCR-based method to differentiate human sex in blood-fed Aedes aegypti (Diptera: Culicidae).","authors":"L Rathod, S Mishra, S Samuel, K Yadav, G Sharma, S Singh, M Kumar, R R Tiwari, S Shubham, D K Sarma","doi":"10.47665/tb.41.2.012","DOIUrl":"https://doi.org/10.47665/tb.41.2.012","url":null,"abstract":"Monitoring mosquito host choice to identify high-risk groups for different vector-borne diseases is important to devise vector control strategies and disease management. The present study was conducted to develop and validate a PCR-based method to identify human sex in blood-fed Aedes aegypti mosquitoes. Several human genes present in both the X and Y chromosomes were screened and diagnostic PCR primers were successfully designed and amplified for the human STS gene. The limit of detection of this PCR assay was carried out on Ae. aegypti fed with human blood up to 5 days (120 hours) post blood-meal under laboratory condition. The efficiency of this PCR assay was evaluated in field-collected Ae. aegypti mosquitoes and compared with other existing methods. The developed PCR primers can successfully amplify and distinguish human sex in mosquitoes up to 72 hours after a blood meal, with an amplified product of 627bp and 298bp for male (XY) and 627bp for female (XX) blood-fed mosquitoes. Further, validation of this assay in field-collected Ae. aegypti mosquitoes revealed that this assay could detect human sex in mosquito blood meal substantially more efficiently (c2 = 4.5, p = 0.034) than other PCR based assay. The newly developed PCR assay highly specific to human DNA and can distinguish male and female DNA for up to 72 hours. This assay can be is used for identifying highrisk groups and extended to other medically important hematophagous insects to assess their role in disease transmission and epidemic preparedness.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"209-213"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Septicaemia due to Vibrio vulnificus: A tropical infection not to be taken lightly. 弧菌引起的败血症：不可掉以轻心的热带传染病。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.004

C H Ding, W A Wahab, M S Saaid, M N Mokhtar, F A Abdullah, I Azaharuddin

引用次数: 0

Inhibition of Plasmodium knowlesi merozoite invasion into human erythrocytes by antibodies raised against the parasite's secreted protein with altered thrombospondin repeat (SPATR). 针对寄生虫具有改变的凝血酶原重复序列分泌蛋白（SPATR）的抗体可抑制克雷疟原虫裂殖体侵入人体红细胞。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.009

U W Azlan, F W Cheong, Y L Lau, M Y Fong

{"title":"Inhibition of Plasmodium knowlesi merozoite invasion into human erythrocytes by antibodies raised against the parasite's secreted protein with altered thrombospondin repeat (SPATR).","authors":"U W Azlan, F W Cheong, Y L Lau, M Y Fong","doi":"10.47665/tb.41.2.009","DOIUrl":"10.47665/tb.41.2.009","url":null,"abstract":"The Plasmodium secreted protein with an altered thrombospondin repeat (SPATR) has been known to play an important role in the malaria parasite's invasion into host erythrocytes. This protein is immunogenic and has been considered as one of the potential vaccine candidates against malaria parasite infection. Thus far, only a handful immunological studies have been carried out on P. knowlesi SPATR (PkSPATR), and none of these studies investigated the immunoprotective properties of the protein. In the present study, the ability of anti-PkSPATR antibodies to inhibit invasion of human erythrocytes was assessed in an in vitro merozoite invasion inhibition assay. The antibodies were harvested from the serum of a rabbit which was immunised with recombinat PkSPATR. Results from the merozoite invasion inhibition assay revealed significant antibody invasion inhibitory activity in a concentration dependent manner (concentration range: 0.375 - 3.00 mg/ml) with inhibition rate ranging from 20% to 32%. Future studies, such as anti-PkSPATR antibodies inhibitory effect on sporozoite invasion of human liver cells, need to be carried out to assess the potential of PkSPATR as a knowlesi malaria vaccine candidate.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"190-195"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tick-borne pathogens isolated from ticks, rodents, and a shrew in Gangwon and Gyeonggi provinces in the Republic of Korea. 从大韩民国江原道和京畿道的蜱、啮齿动物和鼩鼱中分离出的蜱媒病原体。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.007

Y J Choi, J Y Kim, T U Kang, H J Park, H C Kim, I Y Lee, S T Choong, D Y Song, T A Klien, J W Song, W J Jang

{"title":"Tick-borne pathogens isolated from ticks, rodents, and a shrew in Gangwon and Gyeonggi provinces in the Republic of Korea.","authors":"Y J Choi, J Y Kim, T U Kang, H J Park, H C Kim, I Y Lee, S T Choong, D Y Song, T A Klien, J W Song, W J Jang","doi":"10.47665/tb.41.2.007","DOIUrl":"https://doi.org/10.47665/tb.41.2.007","url":null,"abstract":"The prevalence of tick-borne pathogens (TBP), Orientia tsutsugamushi, Rickettsia and Borrelia spp. in wild small animals, namely wild rodents, is now widely investigated. This study is to present the prevalence and distribution of O. tsutsugamushi, Rickettsia and Borrelia spp. in wild small animals and ticks collected from Gyeonggi and Gangwon provinces, Republic of Korea (ROK) in 2014. A total of 131 wild small animals, rodents and shrews, and 2,954 ticks were collected from Gyeonggi and Gangwon provinces from May to November 2014. The wild small animals (KR1-9) and ticks (K1-17) were grouped in accordance with capture dates and locations. Among the wild small animals, a total of 393 tissues and blood samples were extracted from six selected small animal series (KR1-3, KR6-8). Also, each date and location-grouped ticks were identified for its species and pooled according to the stage of development. Molecular identification for Rickettsia, Orientia, and Borrelia species was performed using polymerase chain reaction (PCR). To detect TBPs among wild small animals and ticks, primer sets targeting the 56 kDa protein encoding gene of Orientia spp., outer membrane protein B gene (OmpB) of Rickettsia spp., and 5S-23S intergenic spacer region (IGS) gene of Borrelia spp. were used. Of the 393 wild small animals' blood and tissue samples, 199 (50.6%) were positive for Orientia spp., 158 (40.2%) were positive for Borrelia spp., and 55 (14.0%) were positive for Rickettsia spp. Moreover, a total of 14 tick pools (n = 377) was positive for Rickettsia spp. (n=128, 34.0%) and Borrelia spp. (n=33, 8.8%). High prevalence of Orientia spp. and Rickettsia spp. in rodents and shrews were observed. This study presents significant insights by presenting data collected in 2014 that the prevalence of TBP was already high in mid 2010s. This study highlights the sustainable routine surveillance model for TBP.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"176-182"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia. 马来西亚丁加奴医院相关和社区相关甲氧西林金黄色葡萄球菌临床分离物中毒力基因的分布。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.003

A M Che Hamzah, C C Yeo, S M Puah, K H Chua, A A Rahman, S Ismail, F H Abdullah, N Othman, C H Chew

{"title":"Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia.","authors":"A M Che Hamzah, C C Yeo, S M Puah, K H Chua, A A Rahman, S Ismail, F H Abdullah, N Othman, C H Chew","doi":"10.47665/tb.41.2.003","DOIUrl":"https://doi.org/10.47665/tb.41.2.003","url":null,"abstract":"Staphylococcus aureus is a common bacterial pathogen known to cause various kinds of infections due to its repertoire of virulence factors. This study aimed to investigate the distribution of 19 types of virulence genes among clinical isolates of methicillin-susceptible S. aureus (MSSA) using the polymerase chain reaction. A total of 109 MSSA isolates, i.e., 63 hospital-associated (HA) and 46 community-associated (CA) were collected from Hospital Sultanah Nur Zahirah, the main tertiary hospital in Terengganu, Malaysia, from July 2016 to June 2017. The most frequent virulence genes detected were hla (78.9%, n=86) and hld (78.0%, n=85) encoding hemolysins, lukED (56.9%, n=62) encoding leukotoxin ED, followed by seb (26.6%, n=29) and sea (24.8%, n=27) encoding enterotoxins. Among 34 (31.2%) isolates carrying six or more virulence genes, only five were multidrug resistant (MDR) while the remaining isolates were susceptible. Significant associations were discovered between the hld gene with CA-MSSA (p=0.016) and the seo gene with HA-MSSA (p=0.023). However, there is no significant association between virulence genes among the different types of infection. The clinical MSSA isolates in Terengganu showed high prevalence and high diversity of virulence gene carriage.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"149-156"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence analysis and molecular characterization of low pathogenic avian influenza H9N2 virus isolated from chickens in Sabah. 从沙巴州鸡群中分离出的低致病性禽流感 H9N2 病毒的序列分析和分子特征。

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.008

S A Shohaimi, B L Leow, F F Mohd Yusop, M R Sidik, Z Barker, F H Mohd Saeid

{"title":"Sequence analysis and molecular characterization of low pathogenic avian influenza H9N2 virus isolated from chickens in Sabah.","authors":"S A Shohaimi, B L Leow, F F Mohd Yusop, M R Sidik, Z Barker, F H Mohd Saeid","doi":"10.47665/tb.41.2.008","DOIUrl":"https://doi.org/10.47665/tb.41.2.008","url":null,"abstract":"Low pathogenic avian influenza (LPAI) subtype H9N2 is a causative agent that has raised increasing concern about its impact on poultry and potential public health threats. Even though H9N2 is endemic in Peninsular Malaysia, it was first reported in Sabah in August 2022, after an outbreak associated with high mortality in broiler chickens. In the present study, based on the hemagglutinin (HA) gene, we report the genetic variations and phylogenetic analysis of a H9N2 virus isolated from broiler chickens in Sabah. The sequence analysis of the HA gene revealed a 98% similarity to the H9N2 virus recently isolated from China in 2018. The amino acids in the HA cleavage site displayed a characteristic LPAI motif (PARSSR/ GLF). Notably, at position 226, the isolate had amino acid Leucine (L) demonstrating its ability to bind to the receptor of mammals, resulting in the potential risk of transmission to humans. In addition, the H9N2 isolate harboured seven potential N-glycosylation sites. The phylogenetic analysis revealed that the isolate belonged to clade h9.4.2.5 in the Y280 lineage, similar to previously reported in Malaysia. However, we observed that the isolate in this study falls in a different cluster compared with previous Malaysian isolates, suggesting different source of H9N2 introduction into the country. This prompts us to propose continuous and thorough surveillance of poultry across the country and the necessity of implementing farm biosecurity to minimize economic losses and potential threats to public health.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"183-189"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravaginally CpG-ODN and Salmonella enteritidis on TLR21, cytokines, and AvBD10 gene expressions in the reproductive tract of native chicken. 阴道内CpG-ODN和肠炎沙门氏菌对土鸡生殖道TLR21、细胞因子和AvBD10基因表达的影响

Tropical biomedicine Pub Date : 2024-06-01 DOI: 10.47665/tb.41.2.002

R R B Suryohastari, S H Sumarsono, E A Giri-Rachman, S P Edi

{"title":"Intravaginally CpG-ODN and Salmonella enteritidis on TLR21, cytokines, and AvBD10 gene expressions in the reproductive tract of native chicken.","authors":"R R B Suryohastari, S H Sumarsono, E A Giri-Rachman, S P Edi","doi":"10.47665/tb.41.2.002","DOIUrl":"10.47665/tb.41.2.002","url":null,"abstract":"Salmonella enterica subsp. enterica serovar Enteritidis (SE) is a global concern for the poultry industry due to its association with foodborne illnesses. The transmission occurs through the transovarial route which initiates from colonization in oviducts and ascending to ovaries. Though there are studies on cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) and the increase of innate immune response, there is limited research on the intravaginal treatment using CpG-ODN. Previous studies have shown that stimulating CpG-ODN can induce the production of antimicrobial peptide avian beta-defensins (AvBDs) in vaginal cell cultures, there is limited information on the use of intravaginal treatment to induce the innate immune system, particularly in the Kampung Unggul Balitbangtan (KUB-1) chickens (Gallus gallus domesticus). This study investigates the impact of intravaginal CpG-ODN stimulation on the innate immune response in KUB-1 chicken ovaries and oviducts when challenged to SE. A total of 39 KUB-1 chickens were divided into four groups namely T1 (treated with CpG-ODN, n=12), T2 (SE group, n=12), T3 (CpG-ODN and SE, n=12), and Control (without CpG-ODN and SE, n=3). Chickens were observed from day 1 to 4 post-intravaginal (PI) inoculation. The results suggest that intravaginal CpG-ODN treatment modulates AvBD10 production through toll-like receptor (TLR)21, with interleukin (IL)1B and IL10 playing reciprocal roles, providing insights into the potential of this treatment to prevent transovarial Salmonellosis in poultry. The novelty of this study adds valuable insights to the current body of knowledge.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 2","pages":"142-148"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A colorimetric reverse transcription-loop mediated isothermal amplification (RT-LAMP) method for the rapid detection of SARS-CoV-2 in Thailand. 用于快速检测泰国 SARS-CoV-2 的比色反转录环介导等温扩增（RT-LAMP）方法。

Tropical biomedicine Pub Date : 2024-03-01 DOI: 10.47665/tb.41.1.008

P Bhakdeenuan, S Bunchoo, K Klayut, S Srisungngam, O Suphan, I Kongthap, S Suphankong, B Phetsuksiri, B Uppapong, J Rudeeaneksin

{"title":"A colorimetric reverse transcription-loop mediated isothermal amplification (RT-LAMP) method for the rapid detection of SARS-CoV-2 in Thailand.","authors":"P Bhakdeenuan, S Bunchoo, K Klayut, S Srisungngam, O Suphan, I Kongthap, S Suphankong, B Phetsuksiri, B Uppapong, J Rudeeaneksin","doi":"10.47665/tb.41.1.008","DOIUrl":"10.47665/tb.41.1.008","url":null,"abstract":"COVID-19, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a global health threat. Timely identification of infected cases is important for appropriate patient management and the control of viral spread. Simple and cost-effective tests are required to increase access to testing and early case detection. Here, we describe a colorimetric reverse transcription-loop-mediated isothermal amplification (RT-LAMP) method to detect SARS-CoV-2. The RT-LAMP could amplify the orf1ab sequence detectable by visual color change within 45 min at 63 °C. The limit of detection (LoD) for SARS-CoV-2 RNA was less than 100 copies (13.36) per reaction with no cross-amplification with other related viruses. Clinical evaluation using leftover RNA samples extracted from 163 nasopharyngeal swab specimens showed perfect agreement in negative (n = 124) and positive samples with cycle thresholds (Ct) < 34 cycles (n = 33) detected by real-time reverse transcription-polymerase chain reaction (RT-PCR), targeting RdRp and N genes as a reference. Overall, the diagnostic accuracy, sensitivity, specificity, positive and negative predictive values of RT-LAMP in testing were 96.32% (95% CI: 92.16-98.64%), 84.62% (95% CI: 68.47-94.14%), 100% (95% CI: 97.07-100.0%), 100% (95% CI: 89.42-100.0%), and 95.38% (95% CI: 90.22-98.29), respectively. This RT-LAMP assay is simple and reliable, with the potential to be an alternative for the rapid detection of SAR-CoV-2 with minimal time and fewer resources compared to real-time RT-PCR.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 1","pages":"64-69"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and epidemiological study of some parasitic and viral enteric infections in dromedary camels in Al-Muthanna province. 穆萨纳省单峰骆驼肠道寄生虫和病毒感染的生理学和流行病学研究。

Tropical biomedicine Pub Date : 2024-03-01 DOI: 10.47665/tb.41.1.001

A M R Al-Yasari, N J Alsalih, M A Alsaadawi

{"title":"Physiological and epidemiological study of some parasitic and viral enteric infections in dromedary camels in Al-Muthanna province.","authors":"A M R Al-Yasari, N J Alsalih, M A Alsaadawi","doi":"10.47665/tb.41.1.001","DOIUrl":"10.47665/tb.41.1.001","url":null,"abstract":"Understanding the normal physiology of the body is the key to study the changes that occur due to any infection. It is known that enteric infections play a considerable role in affecting normal body status. Thus, this study was designed for investigating the enteric infections in Arabian camels in Al-Muthanna Province. In this investigation, 588 fecal and blood serum samples (for diarrheic camels only) were collected from the camels in different areas of Al-Muthanna Province, Iraq from both sexes of different ages during the period from October 2020 up to the end of August 2021. The samples were examined using routine microscopic examination techniques, hematological techniques, and ELISA for parasitic and viral identification. Eimeria rajasthani, Isospora orlovi were recorded for the first time in Iraqi camels with clinical signs of diarrhea, dehydration, and emaciation. The study recorded four types of protozoa: Eimeria spp., Isospora, Cryptosporidium and Balantidium coli. The recorded types of Eimeria were E. dromedarii, E. cameli, and E. rajasthani. There was a significant effect of age on infection rates with Eimeria spp. as the highest Eimeria ratio was in ages of less than two years animals. The infection rates were also affected with months which reached the highest ratios of Eimeria in October while the lowest ratio of Eimeria was recorded in July. BVDV infection rate was found in camels that suffered from diarrhea. There is no significant effect of sex on the onset of the viral disease in camels. For hematological parameters, there were significant differences in RBCs, WBCs, Hb, and PCV values in protozoal and BVDV infections. In conclusion, different kinds of protozoal and viral infections were recorded. Some of the recorded infections were associated with acute clinical signs and have zoonotic importance.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection, risk factors, and phylogenetic analysis of tick-borne pathogens in dogs from northern Vietnam. 越南北部犬只中蜱传病原体的分子检测、风险因素和系统发育分析。

Tropical biomedicine Pub Date : 2024-03-01 DOI: 10.47665/tb.41.1.007

T Do, K L Bui, I Zafar, T Inpankaew, M E Galon, P A Ta, K T Tran, T Hasan, J Shengwei, Z Ma, L Hang, M M Amer, Y Ma, K U Mohanta, A E S El Sayed, X Xuan

{"title":"Molecular detection, risk factors, and phylogenetic analysis of tick-borne pathogens in dogs from northern Vietnam.","authors":"T Do, K L Bui, I Zafar, T Inpankaew, M E Galon, P A Ta, K T Tran, T Hasan, J Shengwei, Z Ma, L Hang, M M Amer, Y Ma, K U Mohanta, A E S El Sayed, X Xuan","doi":"10.47665/tb.41.1.007","DOIUrl":"10.47665/tb.41.1.007","url":null,"abstract":"In tropical regions, numerous tick-borne pathogens (TBPs) play a crucial role as causative agents of infectious diseases in humans and animals. Recently, the population of companion and pet dogs has significantly increased in Vietnam; however, information on the occurrence of TBPs is still limited. The objectives of this investigation were to determine the occurrence rate, risk factors, and phylogenetic characteristics of TBPs in dogs from northern Vietnam. Of 341 blood samples tested by PCR, the total infection of TBPs was 73.9% (252/341). Babesia vogeli (18SrRNA gene - 30.5%) was detected most frequently in studied dogs followed by Rickettsia spp. (OmpA gene - 27%), Anaplasma platys (groEL gene - 22%), Bartonella spp. (16SrRNA - 18.8%), Mycoplasma haemocanis (16SrRNA - 9.4%) and Hepatozoon canis (18SrRNA gene - 1.2%), respectively. All samples were negative for Ehrlichia canis and Anaplasma phagocytophylum. Co-infection was detected in 31.4% of the samples (107/341) of which, A. platys/Bartonella spp. (34/94,10%), Rickettsia spp./B. vogeli (19/94, 5.6%), and M. haemocanis/B. vogeli (19/94, 5.6%) were recorded as the three most frequent two species of co-infection types. Statistical analysis revealed a significant correlation between TBP infection and several host variables regarding age, breed, and living area in the current study. The recent findings reported herein, for the first time in Vietnam, are essential for local veterinarians when considering the appropriate approaches for diagnosing these diseases. Furthermore, this data can be used to establish control measures for future surveillance and prevention strategies against canine TBPs in Vietnam.","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"41 1","pages":"52-63"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0