Tropical biomedicine最新文献

{"title":"A case investigation of mortality factors in Japanese Koi (Cyprinus carpio) from a recreational pond.","authors":"B Padilah, B C Kua, A H Noor Hanis, H M Gan","doi":"10.47665/tb.43.3.003","DOIUrl":"https://doi.org/10.47665/tb.43.3.003","url":null,"abstract":"<p><p>An investigation covering water quality, parasitology, bacteriology, and virology was conducted to address cumulative mortality rates exceeding 80% among Japanese koi (Cyprinus carpio) in a recreational pond at AkuaTAR, Fisheries Research Institute, Penang. Low dissolved oxygen levels (DO < 4 mg/L) were identified as a critical environmental stressor likely contributing to the high mortality rates. Gross examinations of the Japanese koi revealed generalised hemorrhages on the body, tail, and fin bases, alongside clinical signs of fin rot and scale loss. The parasite Argulus japonicus was identified based on 18S rRNA with a 100% prevalence and a mean intensity of 79 parasites per fish. Conventional plate culture of the fish and parasite's internal tissue resulted in the isolation of three distinct bacterial strains, identified as Aeromonas sobria, Aeromonas hydrophila, and Enterobacter aerogenes based on biochemical tests. However, further taxonomic validation using whole genome sequencing and in-silico genome-to-genome hybridization approach reclassified both A. sobria and A. hydrophila as Aeromonas jandaei. The presence of multiple bacterial species within the fish parasite indicates its potential role as a vector in disease transmission. The pathology observed in the affected Japanese koi was primarily driven by tissue injury from A. japonicus attachments and bites, and alongside generalised haemorrhage associated with secondary Aeromonas spp. infections. The high parasite burden and tissue damage at the infection site suggest that A. japonicus potentially impairs host defenses and facilitates secondary bacterial infections, ultimately contributing to the mortality event. The findings suggest that the high mortality rate was associated with a multifactorial event involving low dissolved oxygen levels, a severe A. japonicus infestation, and subsequent secondary bacterial infections.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 3","pages":"258-269"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening human brain targets for Japanese encephalitis virus NS5 via yeast two-hybrid assay.","authors":"M E Amir, S Ishak, A R Yasmin, K A Tijani, S S Arshad, S S Hassan, F Mustaffa-Kamal, K Kumar, N Mohamed Sohaimi, S H Nur-Fazila, Y Simonin, S K Loong","doi":"10.47665/tb.43.3.002","DOIUrl":"https://doi.org/10.47665/tb.43.3.002","url":null,"abstract":"<p><p>Japanese encephalitis (JE) is the most important vaccine-preventable acute meningoencephalitis in tropical and subtropical Asia with no known antiviral therapeutics. Available vaccines offer limited protection against emerging Japanese encephalitis virus (JEV) genotypes, posing a persistent public health threat. Disruption of the interactions between JEV non-structural protein 5 (NS5) protein and human brain cellular proteins could be explored to develop flavivirus druggable host targets. This study aimed to identify the human brain cellular proteins that interact with the JEV NS5 protein. A highthroughput yeast two-hybrid (Y2H) screen of a normalized human brain cDNA library was carried out to identify human brain proteins that physically interacted with the JEV NS5 protein. Colony polymerase chain reaction (colony PCR), sequencing and BLAST analysis were carried out to identify and confirm the positive clones. Of the 32 selected large blue colonies on the double dropout media (DDO), only 20 (62.5%) survived on the more stringent selection media (QDO) and retained their blue coloration. Colony PCR, sequencing and BLAST analysis showed that these colonies on a more stringent media carried human brain cDNAs including one uncharacterized cDNA as potential interactors of JEV NS5. Gene Ontology (GO) enrichment analysis identified chemical carcinogenesis-reactive oxygen species, Parkinson's disease, and necroptosis as important biological pathways for JEV neuropathogenesis. The identified JEV NS5-interacting human brain cDNAs highlight the role of NS5 protein and biological pathways such as oxidative stress, Parkinson's disease and necroptosis in JEV neuropathogenesis. These pathways represent potential targets for neuropathologic flavivirus infection.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 3","pages":"251-257"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melioidosis in Asia: Hidden burden, epidemiological distribution, vaccine and therapeutic prospects, and public health priorities.","authors":"M R Afnani, M F A Syahputra, H K Nariswari, R R Riswana, A N M Ansori, T Wibawa, A R Siregar","doi":"10.47665/tb.43.3.005","DOIUrl":"https://doi.org/10.47665/tb.43.3.005","url":null,"abstract":"<p><p>Melioidosis is an under-recognized tropical infectious disease caused by Burkholderia pseudomallei, an environmental pathogen that persists mainly in soil and surface water across tropical and subtropical regions. In Asia, the burden of melioidosis remains poorly defined because many cases are underdiagnosed, underreported, or clinically misclassified as tuberculosis, pneumonia, sepsis, or other bacterial infections. This narrative review examines the epidemiological distribution, transmission pathways, risk factors, vaccine and therapeutic developments, public health challenges, and future control strategies for melioidosis in Asia. Current evidence shows that Southeast Asia remains a major endemic region, while South Asia accounts for a substantial share of the modelled global burden of melioidosis. Reports from East Asia and other ecologically suitable areas suggest potential geographic expansion, particularly in relation to rainfall, flooding, extreme weather events, and climate change. Major risk factors include diabetes mellitus, chronic kidney disease, chronic lung disease, agricultural exposure, low socioeconomic status, and limited access to timely diagnosis and treatment. Although clinical management still depends on prolonged antimicrobial therapy, several vaccine candidates, including live-attenuated, recombinant, glycoconjugate, vesicle-based, and nanoparticle-based platforms, have shown protective effects in preclinical models. However, no licensed vaccine is currently available. Strengthening laboratory capacity, clinician awareness, notifiable disease surveillance, environmental monitoring, and integration with non-communicable disease programs is essential to improve detection and reduce mortality. An integrated One Health framework that links human, animal, and environmental health may support more effective surveillance, prevention, and control of melioidosis across Asia.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 3","pages":"275-286"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic yield of endotracheal aspirate and bronchoalveolar lavage in ventilator-associated pneumonia: A single-centre study.","authors":"M R A Lubis, S Bihar, O M F Harahap, T Ashar","doi":"10.47665/tb.43.3.001","DOIUrl":"https://doi.org/10.47665/tb.43.3.001","url":null,"abstract":"<p><p>Ventilator-associated pneumonia (VAP) remains a significant cause of morbidity and mortality in critically ill patients. This study aimed to compare the diagnostic accuracy of endotracheal aspirate (ETA) with bronchoalveolar lavage (BAL) culture, considered the reference standard, in identifying the causative pathogens of VAP. This study was conducted as a cross-sectional study at Haji Adam Malik General Hospital, Medan, Indonesia. A total of 95 samples from mechanically ventilated patients were analysed using both ETA and BAL in similar institutions. The results demonstrated that 49 samples were positive by both ETA and BAL, while 11 were BAL-positive but ETA-negative, and 31 were ETApositive but BAL-negative. The sensitivity of ETA culture was 81.67%, whereas specificity was 11.43%. The positive predictive value (PPV) was 61.25%, and the negative predictive value (NPV) was 26.67%, yielding an overall diagnostic accuracy of 55.79%. These findings highlight the moderate reliability of ETA in detecting VAP pathogens, with limitations in ruling out infection due to its low NPV. To conclude, the relatively high sensitivity and ease of use suggest that ETA remains a useful initial diagnostic tool, particularly in resource-limited settings, though confirmation with more definitive methods, such as BAL, is recommended to guide appropriate antimicrobial therapy.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 3","pages":"247-250"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of in vitro anthelminthic activity of Indian acalypha (Acalypha indica) root and Indian borage (Plectranthus amboinicus) extract against L3 stage strongyle larvae in sheep.","authors":"S Y Low, P KVasu, D Vellu, A A Nor-Azlina, K F Kasim, M B Sadiq, N S Sofian-Seng, K Juriah, S S Syed-Hussain","doi":"10.47665/tb.43.3.004","DOIUrl":"https://doi.org/10.47665/tb.43.3.004","url":null,"abstract":"<p><p>Gastrointestinal nematode (GIN) infection has been identified as the second most important cause of small ruminant mortality and morbidity in Malaysia. To control GIN infection, commercial anthelminthic drugs (AHD) are used. However, continuous unscrupulous usage of AHD has inevitably resulted in rampant anthelminthic resistance in numerous small ruminant farms in Malaysia. Hence, ethnoveterinary medicine is actively explored to find an alternative to commercial AHD. This study aims to evaluate the anthelminthic activity of Indian acalypha (Acalypha indica) and Indian borage (Plectranthus amboinicus) against third stage strongyle larvae (L3 larvae) in sheep. A total of 300 L3 larvae was assigned to 8 experimental groups (40, 50 and 60 mg/ml Indian Acalypha Root Methanolic Extract (IARME), 10, 30 and 50 mg/ml Indian borage extract (IBE), ivermectin and distilled water). The average percentage of L3 larvae mortality was recorded at time 0, 10 and 30 minutes, 1, 2, 3, 4 and 24 hours. The results revealed that 50 mg/ml IBE is the most efficient treatment, followed by 60 mg/ml IARME. Probit analysis revealed that both IARME and IBE are effective anthelmintic agents with time-dependent increases in efficacy. Therefore, this study showed that IARME might be optimal for rapid treatment, while IBE could be suitable for sustained anthelmintic treatment.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 3","pages":"270-274"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unmasking Bartonella henselae: A forgotten cause of hepatosplenic and marrow involvement.","authors":"U A Zainulabid, X K Cheong, N Kori, J W T Kueh, I Mohamed Rose, R Ramli, M Z Abdul Muien, P Periyasamy, S R Ramli","doi":"10.47665/tb.43.2.012","DOIUrl":"10.47665/tb.43.2.012","url":null,"abstract":"<p><p>Bartonella henselae is an uncommon but important cause of prolonged pyrexia of unknown origin (PUO) and hepatosplenomegaly. Conventional diagnostic tools often fail in disseminated disease, leading to delayed or missed diagnoses. We report a 60-year-old man with PUO, progressive weight loss, and hepatosplenomegaly. Clinical suspicion, guided by significant cat exposure, led to molecular confirmation of B. henselae infection after conventional workup proved inconclusive. Liver biopsy revealed pleomorphic rod-shaped organisms on Warthin-Starry staining, while real-time PCR targeting the rpoB gene and conventional PCR targeting the gltA gene performed on bone marrow aspirate confirmed disseminated bartonellosis. The patient responded well to prolonged doxycycline and rifampicin therapy, achieving near-complete recovery. This case underscores the importance of clinical vigilance, histological clues, and molecular diagnostics in identifying rare but treatable infections.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 2","pages":"219-222"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A soft tissue lesion on the scalp as a peculiar source of disseminated cryptococcosi.","authors":"C H Ding, A A Wahab, N A Abd Rahman, S Zaihan, N Abd Shukor, P Periyasamy, X K Cheong, M N Mokhtar","doi":"10.47665/tb.43.2.003","DOIUrl":"10.47665/tb.43.2.003","url":null,"abstract":"<p><p>Cryptococcosis is a potentially fatal invasive fungal infection with considerable health challenges. A seemingly immunocompetent middle-aged man with frequent exposure to pigeons and bird excreta presented with headaches, fever, gait instability and diplopia. Two years prior to his presentation, he sustained a blunt head trauma which resulted in sporadic headaches. On examination, a hard scalp nodule measuring approximately 3 x 3 cm was palpable on the topmost part of his head. Cryptococcus neoformans was cultured from his cerebrospinal fluid, blood and the biopsied scalp nodule. A diagnosis of disseminated cryptococcosis was made, with the scalp lesion being designated as the primary source of the infection. Following a protracted course of antifungal therapy lasting 10 weeks, the patient was asymptomatic without any neurological deficits when reviewed in the clinic at two months post discharge.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 2","pages":"159-162"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance profiling of Aedes aegypti using intensity concentrations enhances the evaluation of insecticide effectiveness for vector control.","authors":"R Rosilawati, M Norlaili, R Shazreena, A Farah Diana, K Nur Amalina, S Abdul Razak, K L Wan","doi":"10.47665/tb.43.2.014","DOIUrl":"https://doi.org/10.47665/tb.43.2.014","url":null,"abstract":"<p><p>The detection of insecticide resistance is pivotal for characterising the phenotypic resistance of field-collected Aedes aegypti populations, enabling the early identification of resistance trends and safeguarding the efficacy of chemical interventions in vector control programmes. Although the World Health Organization (WHO) adult tube test remains the standard screening method, its reliance on binary classifications of susceptibility and resistance limits the ability to compare the magnitude of resistance among different insecticides. To address this limitation, probit analysis was performed on adult mortality data obtained from a field-collected strain exposed to WHO-recommended discriminating and intensity concentrations. LC50 values were subsequently normalised using a concentration ratio (CR) framework, allowing resistance levels to be quantified and compared across insecticides. The relationship between the CR and slope parameters derived from probit analysis enabled the inference of resistance magnitude within field populations. The results demonstrated that pirimiphos-methyl retained high efficacy against the tested populations, whereas substantial intra-population variation in resistance intensity was observed among pyrethroids and malathion. Lambda-cyhalothrin exhibited the highest level of resistance, followed by permethrin and alpha-cypermethrin. These findings highlight the value of data-driven approaches for enhancing the interpretation of insecticide resistance profiles and providing a more comprehensive assessment of resistance status. By utilising mortality data generated from WHO-recommended discriminating and intensity bioassays, resistance can be evaluated beyond the binary outcomes of standard susceptibility tests, thereby supporting more informed decisions on insecticide rotation and resistance management in vector control programmes.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 2","pages":"231-237"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, risk factor, control and dwelling of Ascaridia galli in the gut of chickens.","authors":"N Khan, W Khan, W Ali, M Shoaib, Anisuzzaman, R Abebe, B S Chaves, V S Vasconcelos, R L R Paludo, Ramos D G S","doi":"10.47665/tb.43.2.002","DOIUrl":"https://doi.org/10.47665/tb.43.2.002","url":null,"abstract":"<p><p>Ascaridia galli infection compromises the health and productivity of chickens. this study aimed to determine the prevalence, pathology, and control of A. galli in backyard chickens. Four hundred and fifty-six faecal samples were tested using direct smear and concentration techniques. sixty chickens were grouped based on egg per gram into untreated control, mebendazole-treated, and derivative-treated. Key epidemiologic host based and environmental variables were recorded and analysed statistically (p < 0.05 significance level). Histopathological changes in the intestine were also assessed. Overall prevalence of A. galli was 72% (330/456). Infection was high in females (74%, 200/267) than in males (68%, 130/189). Adult chickens had a higher infection rate than young (64% vs 55%; p < 0.05). Weak chickens showed a markedly higher prevalence (96%, 150/156) than healthy chickens (60%, 180/300) (p < 0.05). Geographically, prevalence was highest in Swat (85.53%), followed by Lower Dir (72.3%) and Malakand (59.21%), with statistically significant differences (p < 0.05). Management system influenced infection: free range chickens had (63%) compared to semi-free range (37%), scavenging chickens were more infected (75%) compared to receiving supplemental feed (53%) (p < 0.05). The non-dewormed chickens had a significantly higher prevalence (92%) than the dewormed group (20%) (p < 0.05). Seasonal infection peaked during the rainy season (86%) compared to the dry season (44%) (p < 0.05). Intestinal histology revealed inflammation, epithelial necrosis, villous atrophy, and crypt hyperplasia. Treatment of the chickens nematodes using mebendazole resulted in 70% (14/20) of the treated eggs being egg-negative, and the comparison of treatment of the egg-negative derivative compound resulted in 80% (16/20) of the treated eggs being egg-negative. The A. galli infection are very common, and cause intestine damage. The mebendazole and its derivative decrease egg shedding significantly, Husbandry, management and treatment should be enhanced for effective control strategies.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 2","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune dysregulation in herpes zoster: A correlative study of TLR7 gene expression, IFN-a, and CD4/CD8 T-cell.","authors":"R N Alag, H A Al-Hmudi, M K Al-Mishry","doi":"10.47665/tb.43.2.013","DOIUrl":"https://doi.org/10.47665/tb.43.2.013","url":null,"abstract":"<p><p>Varicella-zoster virus (VZV) reactivation or herpes zoster (HZ) results from a decline in cell-mediated immunity. The precise immunological mechanisms driving this reactivation and determining its clinical severity remain unclear. To evaluate the expression of Toll-like receptor 7 (TLR7), serum levels of I interferon response (IFN-a), and the CD4/CD8 T-cell in patients with active HZ and to correlate these immunological markers with disease severity. A case control study was conducted with 50 patients with active HZ and 30 healthy controls. Whole blood and serum samples were collected. TLR7 gene expression was quantified using reverse-transcription quantitative real-time PCR (RT-qPCR), and the serum concentrations of IFN-a, soluble CD4 (sCD4) and soluble CD8 (sCD8) were quantitatively measured by sandwich enzyme-linked immunosorbent assay (ELISA). Patients with HZ exhibited significant upregulation of TLR7 gene expression (p=0.0102) and elevated serum IFN-a levels (p<0.0001) compared with controls. While IFN-a levels did not correlate with clinical severity, both CD4+ (p=0.018) and CD8+ (p=0.016) T cell levels increased significantly with greater disease severity. Sex-specific differences were observed, with males showing higher sCD4 levels and females showing higher sCD8 levels. In conclusion, the adaptive T-cell-derived response, rather than systemic IFN-a levels, is more closely associated with the clinical severity of herpes zoster. The paradoxical upregulation of TLR7 during active disease suggests a complex host-virus interaction involving potential viral evasion mechanisms. These preliminary findings, although limited by sample size, suggest that the sCD4/sCD8 balance and sex-specific immune profiles warrant further investigation as potential prognostic indicators in larger validation cohorts.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 2","pages":"223-230"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148665456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}