Tropical biomedicine最新文献

{"title":"Violet-pigmented Burkholderia contaminans sequence type 922 bacteremia in an end-stage renal disease patient - a case report and review of the literature.","authors":"S Ahmad, N A Mohd Sukhaimi, S S Shahimi, N H Mahfodz, Y H Soh, S AbuBakar, K Y Choong, S K Loong","doi":"10.47665/tb.43.1.001","DOIUrl":"10.47665/tb.43.1.001","url":null,"abstract":"<p><p>Burkholderia cepacia complex (Bcc) is an emerging nosocomial pathogen, with violet-pigmented strains representing a rare and possibly neglected but clinically important subset. We report a case of a 62-year-old man with end-stage renal disease on dialysis who presented with fever and cough. Blood cultures from central and peripheral lines grew violet-pigmented, non-lactose fermenting colonies on MacConkey agar, later identified as Burkholderia contaminans sequence type 922 (ST922) via a multimodal approach. Initial phenotypic methods (VITEK-2, API20NE and MALDI-ToF) and 16S rDNA sequencing were inconclusive; however, multilocus sequence typing confirmed ST922, a strain previously reported in India and associated with nosocomial outbreaks. The isolate was susceptible to ceftazidime, trimethoprim/sulfamethoxazole, minocycline, and meropenem. The patient responded well to intravenous ceftazidime, with subsequent blood cultures yielding no growth. Notably, antimicrobial susceptibility varied geographically, with some ST922 isolates resistant to third-generation cephalosporins. This case illustrates the diagnostic challenges in Bcc speciation and highlights the value of molecular tools such as MLST. The detection of this strain in Malaysia raises concerns about global dissemination, potentially via contaminated medical devices or water systems. Early recognition of violet-pigmented Bcc in resource-limited settings can facilitate prompt, targeted treatment. This report underscores the need for environmental surveillance and antimicrobial stewardship to prevent spread, while reinforcing Bcc's ecological adaptability and clinical significance, especially in immunocompromised individuals.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella in Malaysia: Outbreaks, contamination sources, serovar distribution and antimicrobial resistance patterns.","authors":"S Mhd Jamal, N Philip","doi":"10.47665/tb.43.1.014","DOIUrl":"10.47665/tb.43.1.014","url":null,"abstract":"<p><p>Salmonella is one of the leading causes of foodborne illnesses worldwide, with the ability to contaminate a wide range of sources. The identification of the frequently contaminated sources is vital for improving the management of Salmonella infections and outbreaks. As there is growing concern on bacterial resistance to antimicrobial agents globally, it is crucial to monitor the resistance of Salmonella to antimicrobial agents for effective treatment of salmonellosis and prudent use of antibiotics. The epidemiology of Salmonella, including outbreak patterns, serovars prevalence and antimicrobial resistance profiles, could differ by region. Therefore, assessing the local status of Salmonella is crucial for implementing targeted interventions. This review provides an overview of foodborne disease and Salmonella-related outbreaks in Malaysia, focusing on Salmonella's sources of isolation, serovars and antimicrobial resistance patterns. In Malaysia, food poisoning outbreaks remain a recurring public health issue, and Salmonella is one of the main causative agents with fatal cases. However, the true burden of salmonellosis is difficult to determine as data on non-typhoidal salmonellosis are lacking. Salmonella was detected in both human and non-human samples, with S. Typhimurium and S. Enteritidis being the most common serovars. Salmonella isolates in Malaysia exhibited a wide range of antimicrobial susceptibility patterns, with notably high resistance to erythromycin and penicillin. The review highlights the need for continuous surveillance of Salmonella infections, encompassing both typhoidal and non-typhoidal salmonellosis, as well as systematic monitoring of contamination sources and antimicrobial resistance patterns to improve understanding and ensure effective management of salmonellosis in Malaysia.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 1","pages":"112-131"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of Human Entamoeba histolytica Intestinal Infection in Malaysia: A Systematic Review and Meta-Analysis.","authors":"D Genasan, A Rajamanikam, M Azzani, E Osman, V Subramaniyan, N N M Nazmi, N S A Rahim, N M A Khalip, N A A N Azman, V Kumarasamy","doi":"10.47665/tb.43.1.013","DOIUrl":"10.47665/tb.43.1.013","url":null,"abstract":"<p><p>Entamoeba histolytica (E. histolytica) is a protozoan parasite that causes amoebiasis in humans. It is prevalent in developing countries, particularly in areas with inadequate sanitation and limited access to clean water. While some data on the infection in the Malaysian population is available, comprehensive data on the overall prevalence is lacking. Our study aimed to determine the prevalence of E. histolytica in Malaysia through systematic review and meta-analysis using data published up to 2025. Fourteen studies covering diverse population groups from various states in Malaysia, including rural and urban residents, schoolchildren, indigenous communities, and high-risk populations were reviewed. We found an overall pooled prevalence of 7% with high heterogeneity (I² = 92.5%). Prevalence varied widely by state and population subgroup, with higher rates in Pahang (18%) and among aboriginal schoolchildren (16%). Lower prevalence was found among urban residents (2%) and patients with gastrointestinal disorders (2%). There was only a slight difference in prevalence between individuals with co-infections (8%) and those without (7%). Studies using microscopy showed higher prevalence (7%) than molecular methods (4%). This is likely due to the misidentification of non-pathogenic Entamoeba species as E. histolytica when using microscopy. These findings contribute to a better understanding of the epidemiology of E. histolytica intestinal infection in Malaysia. Although the overall prevalence is relatively low, the results highlight the need for continued surveillance and more accurate diagnostic approaches to support targeted control.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 1","pages":"103-111"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of pyriproxyfen against Aedes aegypti and Aedes albopictus from Penang, Malaysia.","authors":"N A Mohamad, I H Ishak, H A Hasan","doi":"10.47665/tb.43.1.012","DOIUrl":"10.47665/tb.43.1.012","url":null,"abstract":"<p><p>Aedes aegypti and Aedes albopictus mosquitoes are the primary vectors of tropical diseases, including dengue fever (DHF), chikungunya, and Zika, particularly in tropical regions such as Malaysia. Vector control efforts are now increasingly focused on biological and environmentally friendly approaches, one of which is using insect growth regulator (IGR)-based insecticides such as pyriproxyfen. Pyriproxyfen works by disrupting the metamorphosis process of mosquito larvae into adults without causing direct death, but it is effective in inhibiting the emergence of adult mosquitoes by more than 90%, depending on the dose and environmental conditions. This study evaluated the concentration-dependent effects of pyriproxyfen on laboratory and field populations of Aedes spp. Laboratory strains exhibited 100% adult mortality at 1.04 ppm (Ae. aegypti) and 1.08 ppm (Ae. albopictus), whereas field populations required 1.15 - 1.71 ppm depending on locality. LC₅₀ values were lowest in laboratory strains (0.267 ppm for Ae. aegypti and 0.236 ppm for Ae. albopictus), while field strains ranged from 0.326 to 0.571 ppm. LC₉₅ values followed a similar trend, with 1.026 ppm (Ae. aegypti) and 1.107 ppm (Ae. albopictus) in laboratory strains. Inhibition of emergence (IE%) was high in laboratory strains, reaching 97.89% at 0.50 ppm in Ae. aegypti and 63.54% at 0.50 ppm in Ae. albopictus. Field populations required higher concentrations for similar suppression, with IE% ranging from 17.68 - 98.23% in Ae. aegypti and 13.14- 98.24% in Ae. albopictus, peaking at 1.50 ppm. From this finding, it is suggested that pyriproxyfen effectively inhibits adult emergence in both laboratory and field populations, with laboratory strains more sensitive at lower concentrations and field strains responding at higher doses. These results provide strong evidence for pyriproxyfen as an effective, adaptive, and sustainable component of integrated vector management in Malaysia.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 1","pages":"90-102"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous leishmaniasis case caused by the Leishmania donovani complex in northern Cyprus.","authors":"E Ruh, H Ozkayalar, M Nalcaci, S Toz, G Mocan, A Taylan-Ozkan","doi":"10.47665/tb.43.1.008","DOIUrl":"10.47665/tb.43.1.008","url":null,"abstract":"<p><p>The island of Cyprus is located in the Eastern Mediterranean, where leishmaniasis is endemic. Although human visceral and cutaneous leishmaniasis (VL and CL) cases have already been documented on the island, there are limited data on the Leishmania species in northern Cyprus. In this report, we present a CL case diagnosed by both microscopic examination and quantitative real-time PCR (qPCR). The patient, a 79-year-old man residing in northern Cyprus, developed an ulcerative lesion on his left leg. The lesion was surgically excised for histopathological examination, and tissue sections were stained with hematoxylin and eosin (H&E). Microscopic examination of H&E-stained tissue sections revealed Leishmania amastigotes. To confirm the diagnosis and identify Leishmania species at the molecular level, DNA was extracted from the paraffin-embedded tissue sections. Following deparaffinization, qPCR targeting the Leishmania-specific Internal Transcribed Spacer 1 region (located between SSU and 5.8S rRNA genes) was performed. In the qPCR assay, the infecting agent was identified as a member of the L. donovani complex, presumptively L. infantum, based on the melting curve analysis. Our findings provide molecular evidence for the presence of leishmaniasis in northern Cyprus and contribute to addressing the lack of molecular data in the region. Our study also suggests that, due to the zoonotic nature of the identified pathogen, continuous vector and reservoir control programs should be implemented in the region to prevent the spread of the disease.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"43 1","pages":"60-64"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Serological Diagnosis of Strongyloides stercoralis Infection Among Cancer Patients at Hospital Universiti Sains Malaysia.","authors":"A Rahumatullah, N Samsudin, Z Mohamed, R Noordin, A D G Abdullah, Z Baharudeen, M Q A Bakar, N S Anuar, N Z Zakaria, H Hussain Ahmad","doi":"10.47665/tb.42.4.008","DOIUrl":"10.47665/tb.42.4.008","url":null,"abstract":"<p><p>Strongyloidiasis, caused by Strongyloides stercoralis, is a neglected disease with a worldwide prevalence, particularly in tropical and subtropical regions. Most people have chronic asymptomatic infections, which may transform into potentially fatal hyper- or disseminated infections when immunosuppressed. Cancer patients on corticosteroids are at an increased risk of developing severe forms of the infection due to their impaired immune status. The present study used molecular, serological, and parasitological methods to detect S. stercoralis infection in cancer patients on corticosteroids. Using faecal and serum samples from 99 individuals, real-time PCR demonstrated the highest detection rate (27.3%), followed by the Strongyloides IgG4 rapid test (IgG4-RDT or SsRapid) (22.2%) and a commercial IgG-ELISA (4.0%). Agar plate culture performed on 88 of 99 stool samples was negative. There was no significant difference in detection prevalence between the IgG4-RDT and real-time PCR (p = 0.413), and the agreement between them was slight (kappa coefficient, 0.108). Using a composite reference standard (CRS), 41 of 99 samples (41.4%) were classified as positive for Strongyloides infection. Based on the CRS, PCR demonstrated higher sensitivity (65.9%) than IgG4-RDT (53.7%), while both assays exhibited 100% specificity and positive predictive value (PPV). The negative predictive value (NPV) was greater for PCR (80.6%) than IgG4-RDT (75.3%). McNemar's test indicated no significant difference between the two assays (p = 0.49). Notably, combining results of the real-time PCR and IgG4-RDT increased the detection rate to 41%, which was significantly higher than that of PCR alone (27%, p = 0.036) or IgG4-RDT alone (22%, p = 0.0036). The combined results showed substantial agreement with PCR (k = 0.693) and moderate agreement with IgG4-RDT (k = 0.576). In conclusion, the combination of real-time PCR and IgG4-RDT offers a more reliable approach for detecting S. stercoralis in cancer patients undergoing corticosteroid therapy than either assay alone.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"42 4","pages":"422-429"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antiparasitic and synergic effects of camphene combined with pyrimethamine against Toxoplasma gondii.","authors":"H S Al-Aredhi, I A Marhoon, M S Alwan","doi":"10.47665/tb.42.4.004","DOIUrl":"10.47665/tb.42.4.004","url":null,"abstract":"<p><p>Currently, the standard therapeutic approach for the prevention and management of toxoplasmosis is the administration of chemical agents, which are associated with significant adverse side effects. Considering the elucidations presented and the biological properties of camphene (CP), the current research seeks to check the effects and the synergistic interactions of this natural monoterpene against Toxoplasma gondii. The anti-Toxoplsma and synergic effects of CP were examined against T. gondii tachyzoites and its intracellular forms. Furthermore, the impact of CP on the T lymphocytes- associated cytokines and DNA damage- related genes, as well as its effects on the plasma membrane and inducing apoptosis and the nitric oxide (NO) production, were evaluated. CP, principally in conjugation with pyrimethamine (PY), notably (p<0.001) dropped the tachyzoites viability, the intracellular forms, and the rate of infection in macrophages. The obtained FICI values of lower 0.5 for CP and PY indicate synergistic effects when CP is used in conjunction with PY. CP meaningfully upregulated tumor necrosis factor-alpha (TNF-a), interferon-gamma (IFN-g), and interleukin-12 (IL-12), and DNA damage-related genes, as well as NO releases in macrophage cells and the caspase-3 activity within the tachyzoites. The application of CP to tachyzoites displayed a dosage-dependent enhancement in plasma membrane permeability. The selectivity index values for CP, PY, and PY+CP exceeded 10, suggesting their safety for macrophages and specificity toward the parasite. We discovered that CP displayed promising in vitro antiparasitic properties against T. gondii. Additionally, the findings emphasized the potential of CP to eradicate tachyzoites through triggering apoptosis, NO production, and DNA damage, provoking cellular immunity cytokine, and increasing the plasma membrane permeability. Even so, advance evaluation is needed to clarify other mechanisms underlying its action and to evaluate its effectiveness in animal models and clinical trials.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"42 4","pages":"384-390"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal and enzyme-inhibitory effects of essential oils from Eucalyptus globulus and Eucalyptus radiata against Culex pipiens.","authors":"S Toubal, D El Haddad, N Sadaoui, H Aliboudhar, S Boumaza, N Benhabyles, N Chaabane, A Maleki, A Elkeria","doi":"10.47665/tb.42.4.011","DOIUrl":"10.47665/tb.42.4.011","url":null,"abstract":"<p><p>Culex pipiens is among the most abundant mosquitoes in Algeria. This mosquito poses a significant public health risk as a vector of various diseases. Developing efficient and eco-friendly pesticides has also become a highly important issue to reduce the risks associated with conventional insecticides. In this context, the present study aimed to evaluate the responses of fourth-instar larvae of Culex pipiens (C. pipiens) to the effects of two essential oils (EOs) Eucalyptus globulus (E. globulus) and Eucalyptus radiata (E. radiata), both known for their bioinsecticidal properties. The oils were obtained by steam distillation and their chemical composition was determined using gas chromatography coupled to mass spectrometry (GC-MS). Larvicidal bioassays were conducted under laboratory conditions, and in silico analyses were performed to evaluate interactions between the major essential oils constituents and two detoxification enzymes acetylcholinesterase (AChE; PDB ID: 5X61) and glutathione S-transferase (GST; PDB ID: 18GS). E. radiata yielded a higher oil content (1.25 ± 0.36% w/w) than E. globulus (0.92 ± 0.48% w/w). GC-MS profiling revealed marked compositional differences: E. globulus EO was dominated by 1,8-cineole (72.05%), whereas E. radiata contained o-cymene (32.23%) as the main compound. Both EOs showed significant larvicidal activity (p<0.001) against C. pipiens, with E. globulus demonstrating greater potency (LC⁵⁰ = 23.74 ppm) compared to E. radiata (LC⁵⁰ = 53.42 ppm). In silico studies demonstrated that the major constituents of both essential oils exhibit strong insecticidal potential through various interactions with acetylcholinesterase and glutathione S-transferase. Compounds from Eucalyptus globulus showed slightly better binding affinities overall. These results support the development of safe and eco-friendly insecticides, offering a sustainable strategy for protecting both public health and the environment, especially through natural larvicides derived from essential oils targeting Culex pipiens mosquitoes.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"42 4","pages":"446-458"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with melioidosis mortality in Pahang from 2020-2024: data analysis using melioidosis registry from Pahang State Health Department.","authors":"A A Md Yusof, Z I Azhar, A N Mat Rsulin, M F Sazali","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Melioidosis is a severe infection caused by Burkholderia pseudomallei and is endemic in tropical regions, such as Southeast Asia. Favourable climate and substantial agricultural sectors contribute to a high incidence of melioidosis in Pahang, Malaysia. This study aims to identify factors associated with melioidosis mortality in Pahang from 2020 to 2024, with an emphasis on sociodemographic, occupational, clinical, and environmental determinants. A retrospective cohort study was conducted using the state-driven melioidosis registry. All confirmed cases between 2020 and 2024 were included in the analysis. All variables were extracted and analysed using simple and multiple logistic regression to identify the risk factors for melioidosis mortality. Out of 466 confirmed cases, 68 (14.6%) died from melioidosis. The median age was 48 years (IQR: 34-60), with the majority being male (76%) and originating from rural areas (71%). The incidence, mortality and case fatality rates fluctuated over the study period. The final regression model analysis identified three risk factors for mortality which include non-Malaysian ethnicity (aOR 3.27; 95% CI: 1.43-7.51), chronic obstructive pulmonary disease (COPD) (aOR 8.71; 95% CI: 1.11-68.39), and intensive care unit (ICU) admission (aOR 3.44; 95% CI: 1.91-6.17). In contrast, diabetes mellitus, which is often associated with increased risk of mortality, was not found to be significant in this study, nor was the level of monthly rainfall. These study findings highlight the need to address social determinants of health among vulnerable populations and to prioritise early recognition and management, especially for patients with COPD.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"42 4","pages":"415-421"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric tetra-combo loop-mediated isothermal amplification assay for efficient SARS-CoV-2 detection.","authors":"M S Chow, B P Kee, P C Lee, K H Chua","doi":"10.47665/tb.42.4.002","DOIUrl":"10.47665/tb.42.4.002","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly and caused a pandemic in 2020. Both pharmaceutical approaches, such as vaccinations and non-pharmaceutical approaches such as social distancing through lockdown and international border restrictions had been implemented to control the outbreak and the transmission of SARS-CoV-2. However, the prolonged implementation of these measures negatively impacted the population and global economy, while the continuously emerging SARS-CoV-2 variants led to breakthrough infections among the vaccinated populations. Given the ongoing nature of COVID-19, an efficient detection method for SARS-CoV-2 that could enable mass screening and on-site screening is needed to manage the disease and prevent further outbreaks at large scale. Hence, we have developed a tetra-combo LAMP SARS-CoV-2 detection assay that targets four SARS-CoV-2 genes (RdRp, S, E and N), with an internal control (RNaseP). The LAMP assay was validated using 370 RNA samples extracted from nasopharyngeal and/or oropharyngeal swabs. The LAMP assay developed in this study has on-par performance as the qRT-PCR assay, which is the gold standard method for SARS-CoV-2 detection, with a shorter turnaround time (25 minutes). The LAMP assay possessed 98.13% sensitivity, 100% specificity, 100% positive predictive value (PPV), and 95.45% negative predictive value (NPV) for samples with Ct values <= 35.</p>","PeriodicalId":101343,"journal":{"name":"Tropical biomedicine","volume":"42 4","pages":"370-378"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}