Se pu = Chinese journal of chromatography最新文献

{"title":"[Recent advances in porous materials based silica stationary phases for liquid chromatography].","authors":"Ze-Xin Huang, Sheng Tang","doi":"10.3724/SP.J.1123.2025.12022","DOIUrl":"10.3724/SP.J.1123.2025.12022","url":null,"abstract":"<p><p>Recent years have seen significant progress in nanoscience and materials chemistry， driving the development of novel porous materials such as metal organic frameworks （MOFs）， covalent organic frameworks （COFs）， and porous organic cages （POCs）. MOFs form through coordination bonds between metal ions and organic bridging ligands， COFs result from strong covalent linkages connecting predesigned organic building blocks into extended crystalline networks， and POCs are three-dimensional cage-like organic molecules with well-defined intrinsic cavities. Nowadays， the use of porous materials as stationary phases in high-performance liquid chromatography （HPLC） continues to draw sustained research interest. By precisely designing the building blocks of porous materials， researchers can flexibly modulate separation selectivity in terms of pore size， pore morphology， and interlayer stacking patterns， thus enabling the efficient separation of complex samples， particularly for positional isomers and chiral compounds. Despite their considerable promise for chromatographic applications， the direct use of porous materials as stationary phases is constrained by inherent limitations. A primary challenge lies in synthesizing pure porous materials with both regular morphology and uniform particle size. Irregular particle shapes combined with broad size distributions typically result in high column pressure and low column efficiency， which severely restricts the practical application of porous materials in HPLC. To overcome these limitations， combining porous materials with silica gel （SiO<sub>2</sub>） supports has become a widely adopted strategy. As the most mature stationary phase support in HPLC， SiO<sub>2</sub> offers excellent mechanical strength， chemical stability， and a readily functionalizable surface， thereby providing a robust structural platform for porous materials. By immobilizing porous materials onto the silica surfaces， their mechanical robustness and packing uniformity can be significantly improved while retaining their selective adsorption capability， thus promoting their reliable use in chromatographic applications. Furthermore， in order to further enhance separation efficiency and selectivity， researchers have proposed the co-modification of silica gel with porous materials and other functional components such as ionic liquids， hydrogels， and carbon dots， aiming to construct multifunctional composite stationary phases. The key advantage of this strategy lies in the synergistic integration of multiple separation mechanisms， including hydrophobic effects， hydrogen bonding， <i>π-π</i> interactions， and chiral recognition， through material hybridization， thereby achieving higher performance and selectivity in separating complex systems. Moreover， this composite approach effectively mitigates the limitations of single-component materials， for example， by improving the aqueous stability of MOFs or extending the applicability of COFs in the analys","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 10","pages":"1118-1128"},"PeriodicalIF":0.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recent progress in constructions and applications of chiral porous framework-based chromatographic stationary phases].","authors":"Shen Long, Jia-Yu Chen, Chen Yuan, Xian-Deng Hou","doi":"10.3724/SP.J.1123.2026.01009","DOIUrl":"10.3724/SP.J.1123.2026.01009","url":null,"abstract":"<p><p>Chirality， as an inherent property of nature， plays a central role in the complexity and precision of biological systems. The stereochemistry of molecules profoundly impacts their pharmacological activities， metabolic pathways， and toxicological effects. Enantiomers， which are mirror-image forms of the same compound， often exhibit significantly different behaviors， making the accurate separation and trace analysis of racemates crucial in modern pharmaceutical research， as well as in the production of high-value chiral intermediates. The precise enantioseparation of compounds has therefore become an essential component of drug development， environmental monitoring， and chemical synthesis. Among various separation methods， chiral chromatography has emerged as the most dominant technique due to its high efficiency， versatility， and rapid analytical capabilities. Conventional chiral stationary phases （CSPs） have been primarily based on materials such as polysaccharides， proteins， and synthetic polymers. However， recent advancements in materials science have led to the development of chiral porous frameworks， particularly metal-organic frameworks （MOFs） and covalent organic frameworks （COFs）， which offer remarkable advantages. These materials feature ultra-high surface areas， well-ordered porous structures， and tunable chiral microenvironments， making them ideal candidates for the next generation of chiral stationary phases in chromatography. This review provides a comprehensive summary of the recent progress made by domestic scholars from 2023 to 2025 on the application of MOFs and COFs in chiral separation， specifically in high performance liquid chromatography （HPLC）， gas chromatography （GC）， and capillary electrochromatography （CEC）. It begins by discussing the construction strategies for these chiral porous frameworks， highlighting their synthesis and functionalization methods. Next， it focuses on the preparation techniques for incorporating these materials into chromatographic stationary phases， emphasizing the challenges and innovations involved in optimizing their performance. The review then examines the practical applications of MOFs and COFs in the separation of racemates， showcasing their use in the enantioseparation of pharmaceuticals， agrochemicals， and natural products. The separation performance is evaluated based on key metrics such as resolution， selectivity， and enantioselectivity. Comparisons with traditional commercial columns highlight the superior performance of MOF- and COF-based stationary phases， including their higher efficiency， broader selectivity， and better adaptability to a wider range of compounds. Finally， the review discusses the future directions and challenges in the field. These include the need for scalable synthesis methods， enhancing the stability of MOFs and COFs under practical chromatographic conditions， and improving the selectivity of these materials. Future research may also focus on hybrid MOF-COF ","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 10","pages":"1105-1117"},"PeriodicalIF":0.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Structural features and regulation of adsorption/separation properties of photoresponsive metal-organic frameworks].","authors":"Li-Meng Liu, Wen-Zhe Xie, Yan-Ying Qu, Tian-Pei Cai, Dong-Hao Li, Hai-Bo Shang","doi":"10.3724/SP.J.1123.2026.01025","DOIUrl":"10.3724/SP.J.1123.2026.01025","url":null,"abstract":"<p><p>Metal-organic frameworks （MOFs） are attractive porous materials with periodic network structures， which are constructed via the self-assembly of metal ions （or metal clusters） and bridging organic ligands. MOFs have some unique advantages of designability， ordered pore structure， high specific surface area and modifiability. Photoisomerization refers to the phenomenon in which， under light of specific wavelengths， the molecular structures of certain compounds undergo reversible interconversion due to photoexcitation. Both isomeric forms can exist stably under certain conditions and exhibit distinct chemical and physical properties. On this basis， photoresponsive MOFs （PMOFs） are proposed as a class of smart materials constructed by integrating photoactive molecules into MOFs. PMOFs combine the inherent superior porous structure of MOFs with the dynamic tunability enabled by photo-induced isomerization and exhibit unique advantages in many fields， including catalysis， drug release， separation， anti-counterfeiting， etc. PMOFs， for instance， can precisely regulate adsorption/separation behaviors and enable green， energy-efficient recovery of adsorbents under photoinduction， which has attracted widespread attention. This review summarizes common types and properties of photoactive molecules， including azobenzene， spiropyran， diarylethene， spirooxazine， fulgide， Schiff base， etc.， as well as three main integration strategies of photosensitive units/molecules with MOFs frameworks. Backbone incorporation involves using the entire photoactive organic molecule as an organic ligand within the MOF framework. For side-group grafting， the photoactive moiety is attached as a side group or through post-synthetic modification of the organic ligand， effectively grafting it onto the MOF backbone. Guest encapsulation is referred to embedding photoactive molecules as independent guest species within the cavities or pores of the MOF. By analyzing the differences in isomerization of various photoactive groups and the structural features of different integration modes， the multidimensional modulations of MOF are illustrated in pore size， surface properties， host-guest interaction， \"hiding\" and \"exposure\" of adsorption active sites， and framework geometry via photoisomerization. Furthermore， from a structure-performance perspective and with representative case studies， this review discusses the regulatory mechanisms and application potential of PMOFs in the adsorption and separation of small-molecule gases， metal ions， and organic dyes. The key limitation of PMOFs lies in whether the MOF framework is robust enough to tolerate the molecular mechanical forces produced by photoswitch configurational rearrangements. The cyclic photo‑switching performance of PMOFs is often unsatisfactory， which is primarily attributed to the molecular mechanical forces generated during repeated photoisomerization， which may cause local microscopic distortion， phase transitions， or even","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 10","pages":"1150-1161"},"PeriodicalIF":0.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Simultaneous determination of three tryptamines in foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with hydrophilic column separation].","authors":"Xiao-Lei Liao, Qin Lin, Yue Mei, Hong-Shun Dou, Hui Xu, Yun He, Cheng Pan, Feng-Ling Jiang","doi":"10.3724/SP.J.1123.2025.08022","DOIUrl":"10.3724/SP.J.1123.2025.08022","url":null,"abstract":"<p><p>The illegal addition of substances in ordinary food poses a serious threat to food safety. Therefore， the detection and identification of such additives have long been important research areas in the field of food safety. Especially， illegal additives are showing new trends now， such as emerging functional varieties， new structural derivatives， and clandestine addition practices. Meanwhile， along with the development of online shopping and logistics， new threats pose a substantial challenge to social governance and food safety. In recent years， there are frequent cases of people consuming wild mushrooms containing hallucinogenic tryptamine toxins out of curiosity， and the abuse of tryptamine psychoactive substances also occurs from time to time. Therefore， the development of a robust and sensitive analytical method is necessary for accurately identifying the risks associated with tryptamine psychoactive substances.To address this issue， a novel method based on ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） combined with an isotopic internal standard and solid-phase extraction purification technique was developed for the simultaneous determination of three tryptamines （psilocin， bufotenine and psilocybin） in ten types of ordinary foods. Several optimization procedures were undertaken， involving the chromatographic conditions， mass spectrometric parameters， and sample extraction and purification protocols. The food samples were extracted and centrifuged， followed by purification using a strong cation exchange solid-phase extraction column. The separation was carried out using a Capcell PAK ST （S-5） hydrophilic interaction chromatographic column. The multi-reaction monitoring （MRM） mode was employed for data acquisition in the positive ion mode， whereas isotopic internal standards were used to quantify the three tryptamines. It was verified that the calibration curves of psilocin， bufotenine， and psilocybin exhibited good linearities （<i>R</i><sup>2</sup>>0.995） within the range of 0.025-10.0 ng/mL. The limits of detection （LODs） for psilocin， bufotenine and psilocybin were determined as 0.4， 0.5， and 0.6 µg/kg， respectively， while the limits of quantification （LOQs） were determined as 1.4， 1.7， and 1.9 µg/kg， respectively. The LODs and LOQs demonstrate that the method has the ability to detect minute quantities of tryptamines. The recoveries of the tryptamines were 94.3%-113.7% under three spiked levels， with relative standard deviations （RSDs） ranging from 0.4% to 4.9% （<i>n</i>=6）. The consistent and satisfactory recoveries of tryptamines indicate that this method meets the requirements to accurately quantify the target analytes even in a complex matrix. In summary， this newly developed method is rapid， sensitive， accurate， and compliant with relevant regulatory standards； hence， it is suitable for the determination of psilocin， bufotenine and psilocybin in food matrices. As part of the application of this met","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"1010-1018"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Teaching practice of instrumental analysis course based on inquiry-based learning and critical thinking： a case study of competency development for pharmacy students].","authors":"Ding Bo","doi":"10.3724/SP.J.1123.2025.09002","DOIUrl":"10.3724/SP.J.1123.2025.09002","url":null,"abstract":"<p><p>Instrumental analysis is a crucial foundational course for pharmacy majors， and its teaching effectiveness directly relates to the cultivation of students' analytical testing abilities， scientific research literacy， and innovative thinking. Current teaching commonly faces problems such as complex content systems， difficulty in understanding principles， and disconnection between theory and experiments， resulting in students' lack of active inquiry awareness and critical thinking. To address these issues， this study takes pharmacy students from 2019-2023 classes at the School of Pharmacy， Guangzhou Medical University as subjects， exploring a teaching model that combines inquiry-based teaching with the cultivation of critical thinking. By constructing a stepwise inquiry approach that progresses from imitative to comparative to case-based learning and designing problem-oriented learning tasks and reflective assessment mechanisms， the study aims to guide students in achieving knowledge construction and cognitive enhancement within authentic problem contexts. Teaching practice results indicate that this model effectively promoted students' transition from passive acceptance to active inquiry， significantly improving their analytical testing abilities， scientific research literacy， and innovative capabilities. However， some students still exhibited habitual thinking in knowledge transfer and analysis strategy selection， and the depth of critical reasoning needs to be further strengthened. This study provides a systematic pathway and empirical evidence for the teaching reform of instrumental analysis courses， and is of significant importance for cultivating high-quality pharmacy professionals with innovative capabilities.</p>","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"1090-1099"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Determination of glufosinate and its metabolite residues in tea by ultra-high performance liquid chromatography-tandem mass spectrometry coupled with precolumn derivatization].","authors":"Rui-Feng Bi, Shi-Hui Fu, Suo-Cheng Deng, Meng Fu, Wei-Wei Zhang, Yu-Hua Song","doi":"10.3724/SP.J.1123.2025.09022","DOIUrl":"10.3724/SP.J.1123.2025.09022","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Tea is an important cash crop in China， and pesticide residues in tea products are among the main issues affecting tea exports. Glufosinate （GLU） is a widely used herbicide in tea gardens and its residues in tea samples have raised health concerns. However， there are few reports on analytical methods for the simultaneous determination of GLU and its metabolite residues in tea. Therefore， the development of suitable analytical methods to monitor the content of GLU and its metabolite residues in tea is essential to ensure compliance with the export quality standards of tea products. In this study， a sensitive analytical method was developed for the determination of GLU and its two main metabolites， &lt;i&gt;N-&lt;/i&gt;acetyl-glufosinate （NAG） and 3-［hydroxy（methyl）phosphinoyl］propionic acid （MPP）， in tea. The method is based on ultra-high performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） coupled with precolumn derivatization. Tea samples （2.00 g） were extracted with 20 mL of ultrapure water by shaking， and followed by centrifugation. After freezing treatment， aliquots of the extract were centrifuged at 15 000 r/min. Then 200 μL of the supernatant was evaporated to complete dryness using nitrogen blowdown. The resulting residue was derivatized with triethyl orthoacetate （TEOA） at 80 ℃ for 1.5 h， in the presence of acetic acid as the catalyst. The derivatization mixture was evaporated to dryness and reconstituted with methanol. NAG was transformed into the same reaction product as GLU， and therefore quantified together with GLU. The derivatization products were separated on an Agilent ZORBAX Eclipse Plus C18 column （100 mm×2.1 mm， 1.8 μm）. The mobile phase consisted of an aqueous solution of 0.1% （&lt;i&gt;V/V&lt;/i&gt;） formic acid （containing 5 mmol/L ammonium formate） as phase A and acetonitrile as phase B. The gradient elution was carried out as follows： 0-0.5 min， 90%A； 0.5-7.5 min， 90%A-80%A； 7.5-8.0 min， 80%A-10%A； 8.0-10.0 min， 10%A； 10.0-10.1 min， 10%A-90%A； 10.1-12.0 min， 90%A. The separated compounds were detected by tandem mass spectrometry with positive electrospray ionization （ESI&lt;sup&gt;+&lt;/sup&gt;） in multiple reaction monitoring （MRM） mode， and stable isotope-labeled internal standards were used for quantitative analysis. Good linearity was obtained in the 0.5-20 μg/L range； the correlation coefficients （&lt;i&gt;r&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt;） were higher than 0.995， the limits of detection were 2.2-5.3 µg/kg and the limits of quantification were 25 µg/kg. For two representative matrices of green tea and black tea， the average recoveries of GLU and MPP spiked at four levels of 0.025， 0.05， 0.1， and 0.5 mg/kg were 83.9%-105.0%. The intra-day precisions （&lt;i&gt;n&lt;/i&gt;=5） ranged from 1.9% to 6.9% and the inter-day precisions （&lt;i&gt;n&lt;/i&gt;=10） ranged from 2.6% to 10.3%. Furthermore， the developed method was used to detect GLU and its metabolite residues in 34 tea samples， and the positive detection ratio was 26.5%. In summary， the proposed method is simple， reliabl","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"1019-1027"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Self-designed experiment in instrumental analysis： online solid phase extraction-high performance liquid chromatography- post column derivatization for the determination of trace pesticide residues in vegetables].","authors":"Yan Li, Yun-Tao Liu, Xien Huang, Zi-Yi Zhou, Zi-Qiu Wang, Jing Wang, Fei Ding, Jing Nan, Anna Tang","doi":"10.3724/SP.J.1123.2025.10020","DOIUrl":"10.3724/SP.J.1123.2025.10020","url":null,"abstract":"<p><p>In line with modern educational concepts and the demand for innovative talents， a course focused on self-designed experiments has been incorporated into the traditional instrumental analysis curriculum. This initiative aims to enhance students' ability to apply knowledge effectively. While mastering instrument operation， students are encouraged to anticipate potential challenges during experiments. Under instructor guidance， students constructed an online system for solid-phase extraction coupled with liquid chromatography and post-column derivatization with fluorescence detection. They addressed real analytical needs such as improving sensitivity and precision， minimizing interference， and conducting rapid analyses of trace carbamate pesticides in vegetable samples. This self-designed liquid chromatography experiment course， tailored to students' learning characteristics and interests， successfully engages their initiative and fosters independent innovation capabilities. As a result， it also improves teaching effectiveness and enhances learning efficiency.</p>","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"1074-1081"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recent advances on environmental occurrence， risk and analytical methodologies of synthetic phenolic antioxidants].","authors":"Si-Si Liu, Zi-Jun Li, Hong-Bo Wei, Piao-Li Xu, Li-Na Li, Chang-Er Chen, Guang-Guo Ying","doi":"10.3724/SP.J.1123.2026.02013","DOIUrl":"10.3724/SP.J.1123.2026.02013","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Synthetic phenolic antioxidants （SPAs） are a major class of chemical additives extensively employed in plastics， rubber， and food packaging to prevent polymer degradation and extend product lifespans. In recent years， the environmental and human health risks of SPAs have received great concern due to their widespread occurrence in various environmental media. Existing reviews primarily focus on environmental detection， human exposure， toxicity， and risk assessment of SPAs with limited discussion on recent advances in analytical methodologies. This review presents a systematic synthesis of research advances over the past five years， especially the state of the art analytical methodologies. This review systematically analyzes their occurrence in water， sludge， sediments， soils， dust， and biota tissues， revealing a clear pollution transition from conventional SPAs to emerging high-molecular-weight substitutes. Toxicological evidence demonstrates that SPAs exert adverse effects on rats， mice， and aquatic organisms， including genotoxicity， immunotoxicity， endocrine disruption， reproductive toxicity， etc. Notably， certain transformation products （TPs） of butylated hydroxytoluene （BHT） exhibit enhanced toxicity relative to their parent compounds. Methodological advances in sample preparation and analysis techniques of SPAs in different environmental matrices are reviewed for the first time. Recent applications of atmospheric pressure chemical ionization coupled with liquid chromatography-tandem mass spectrometry （APCI-LC-MS/MS）， as well as high-resolution mass spectrometry （HRMS） provide powerful approaches for target/non-target analysis of unknown SPA derivatives and TPs. Several critical research gaps have finally been identified. Environmental analytical methods of SPAs and their TPs face significant challenges due to their diverse physicochemical properties and chemical reactivity， particularly their susceptibility to oxidation. Another key issue is that current pretreatment approaches for SPAs in tissue samples overlook bound-SPAs， thereby underestimating their real body burdens. Moreover， existing studies mainly focus on traditional SPAs， while lacking systematic assessment of environmental fate and long-term ecological risks posed by emerging SPAs and potentially more toxic TPs. Additionally， ecotoxicological data of SPAs for terrestrial organisms are scarce compared to aquatic organisms and mammals， limiting comprehensive ecological risk assessment. Future research should prioritize： （1） developing oxidant-resistant pretreatment methodologies coupled with HRMS to overcome technical challenges in accurate quantification of trace SPAs and their bound species； （2） strengthening non-target screening and identification of emerging SPAs and TPs； （3） investigating long-term effects of SPAs in soil-plant-microorganism systems. This review advances our understanding of SPAs by bridging the knowledge gaps and providing updated insights into current ","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"991-1000"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research highlight in molecular imprinting recognition of phosphopeptide isomers].","authors":"Luxi Chen, Hailiang Hu","doi":"10.3724/SP.J.1123.2026.08007","DOIUrl":"10.3724/SP.J.1123.2026.08007","url":null,"abstract":"","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"989-990"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of chiral separation performance of （2-hydroxyethyl-<i>β</i>）-cyclodextrin bonded superficially porous and fully porous particle chiral stationary phases in supercritical fluid chromatography and reversed-phase liquid chromatography].","authors":"Guang-Yong Yang, Hai-Jiang Wu, Huang-Tao Zhang, Hong-Li Wang, Qiu-Yan Liang","doi":"10.3724/SP.J.1123.2025.12017","DOIUrl":"10.3724/SP.J.1123.2025.12017","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Chromatography remains a cornerstone technique in analyzing chiral compounds. The development and selection of chiral stationary phases （CSPs） are central to achieving high-resolution enantiomeric separation. This study presents a novel regeneration and functionalization strategy for the preparation of high-performance superficially porous particle （SPP）-CSP， offering a promising alternative to conventional fully porous particle （FPP）-CSP. First， bonded phases on the surface of spent chromatographic column silica packing materials were removed using ultraviolet light （&lt;i&gt;λ&lt;/i&gt;=254 nm） and hydrogen peroxide solution （3%， mass fraction）， thereby obtaining sub-3 μm regenerated SPP. The regenerated SPP were then amino-functionalized. Subsequently， under mild reaction conditions， carbonyldiimidazole-activated （2-hydroxyethyl）-&lt;i&gt;β&lt;/i&gt;-cyclodextrin （HE-&lt;i&gt;β&lt;/i&gt;-CD） was bonded onto the surface of the amino-functionalized SPP to prepare the SPP-CSP. For comparative evaluation， a CSP based on 3 μm commercial FPPs was prepared. Additionally， a commercial （2-hydroxypropyl）-&lt;i&gt;β&lt;/i&gt;-cyclodextrin （Hp-&lt;i&gt;β&lt;/i&gt;-CD） chiral column with identical particle size and structure was selected as a reference. This commercial column was used to investigate the selectivity differences among different cyclodextrin derivatives. The enantioseparation and overall chromatographic performance of the CSPs were systematically evaluated under both supercritical fluid chromatography （SFC） and reversed-phase liquid chromatography （RPLC） modes. The evaluation was performed using ten chiral molecular probes： zopiclone， equol， 2-phenylpropionic acid， dobutamine， flutriafol， miconazole， folic acid， catechin， valacyclovir， and chlorphenamine. Results revealed that， under identical chromatographic conditions， the SPP-CSP consistently outperformed the FPP-CSP in terms of enantiomeric resolution， peak sharpness， theoretical plate number， and retention time efficiency. More importantly， the FPP-CSP exhibited a significant loss in resolution when chromatographic conditions were altered to yield equivalent analysis times. In contrast， the SPP-CSP showed only minor adverse effects on resolution when the flow rate was increased to shorten analysis time， highlighting its inherent suitability for ultrafast separations. Moreover， SFC and RPLC modes displayed complementary chiral recognition ranges. Within the overlapping separation scope of both modes， SFC mode achieved higher enantiomeric resolution and superior chromatographic performance. Notably， under RPLC mode， catechin showed high enantiomeric resolution on both CSPs， underscoring system-dependent selectivity differences. Multiple factors-including mobile phase composition （particularly the additive type）， and the stereochemical structure of the analytes-were found to significantly influence enantioseparation outcomes. Under SFC mode， none of the analytes could be eluted when acetonitrile was used as the mobile phase modifier， whereas a","PeriodicalId":101336,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"44 9","pages":"1001-1009"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}