Revista del Laboratorio Clínico最新文献_第6页

Evaluación del comportamiento en términos de error total y 6Sigma y estimación de la incertidumbre de medida de 16 magnitudes de bioquímica clínica 根据总误差和6西格玛评估行为，并估计16个临床生化量的测量不确定度

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2019.01.001

Carolina Bignone, Eugenia Osinde, Maria Cecilia Pace, Irina Maggioni Blanco, Antonela Molfese, Viviana Osta, Sandra Ayuso

{"title":"Evaluación del comportamiento en términos de error total y 6Sigma y estimación de la incertidumbre de medida de 16 magnitudes de bioquímica clínica","authors":"Carolina Bignone, Eugenia Osinde, Maria Cecilia Pace, Irina Maggioni Blanco, Antonela Molfese, Viviana Osta, Sandra Ayuso","doi":"10.1016/j.labcli.2019.01.001","DOIUrl":"10.1016/j.labcli.2019.01.001","url":null,"abstract":"<div><p>Within the analytical quality system of the clinical analysis laboratories, it is usual to calculate Total Error (ET) and Six sigma (6Sigma). The estimation of the measurement uncertainty (U) is a parameter that should be incorporated as part of the quality management, and is a requirement of ISO 15189. The U provides a range of probable values where the true value of a measurement result can be obtained, providing a quantitative value of the level of doubt for each value. The objective of this work is to evaluate the performance of 16 analytical methods using the calculation of the ET and the 6Sigma, as well as the U, based on an approximation model of the Nordtest guide. Internal (CCI) and external quality control (EQA) data were used. Quality requirements (ETa) based on CLIA and biological variability (BV) were used to evaluate the performance of the methods. The 16 methods presented acceptable performance, with the ET values obtained being lower than the proposed ETa and the 6Sigma values≥3. Three methods have values of 6Sigma between 3 and 4, 2 methods between 4 and 5Sigma, five values between 5 and 6Sigma, and six had 6Sigmas greater than 6. The uncertainties associated with all measurements provide complementary information about the range of values in which the true value is found.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77163117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seudohiperfosfatemia analítica como punto de partida en el diagnóstico de una disproteinemia 分析性假高磷血症作为诊断蛋白血症的起点

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/J.LABCLI.2018.03.003

Carlos Castillo‐Pérez, Carmen Oana Minea, Marta Cebrián Ballesteros, L. R. Alonso, Blanca Torrubia Dodero, Inmaculada Martín-Mérida

引用次数: 0

¿Cuáles son las claves de la granulomatosis linfomatoide con afectación extrapulmonar? 淋巴样肉芽肿伴肺外受累的关键是什么?

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2018.07.002

Inmaculada Ruiz Molina , M. José Llamas Poyato , Eduardo Solís García

引用次数: 0

Citopatología y bioquímica del líquido pleural reumatoideo. A propósito de un caso 类风湿胸膜液的细胞病理学和生物化学。关于一个案例

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/J.LABCLI.2018.07.001

F. P. Codeso, María Pilar Pérez Soriano, E. Miranda, Antonio Dorado Galindo, M. Ojeda

引用次数: 1

Eficacia y optimización de la citometría de flujo en el cribado universal de la infección del tracto urinario 流式细胞术在尿路感染通用筛查中的有效性与优化

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2018.12.004

Patricia Gallego Anguí , Juan Cuadros González , Juan Romanyk , Peña Gómez Herruz , Rosa González , Teresa Arroyo , José Vicente Saz

{"title":"Eficacia y optimización de la citometría de flujo en el cribado universal de la infección del tracto urinario","authors":"Patricia Gallego Anguí , Juan Cuadros González , Juan Romanyk , Peña Gómez Herruz , Rosa González , Teresa Arroyo , José Vicente Saz","doi":"10.1016/j.labcli.2018.12.004","DOIUrl":"10.1016/j.labcli.2018.12.004","url":null,"abstract":"<div><h3>Introduction</h3><p>Flow cytometry has shown to be useful for ruling out urinary tract infection over the last few years. Its integration into the Microbiology Laboratories could avoid the urine culture of 60% of the samples. The aim of this study is to evaluate the usefulness of flow cytometry in the universal screening, as well as to improve its efficacy by using specific cut-off points in different groups.</p></div><div><h3>Material and methods</h3><p>A total of 1338 urine samples were analysed by flow cytometry (Sysmex UF-1000i), as well as a urine culture in CPS agar. Cultures with one or two pathogens and more than 10,000 CFU/ml, and special cases with less counts but just one pathogen, were considered as positive.</p></div><div><h3>Results</h3><p>A cut-off of >17.1 bacteria/μl or >29.5 leucocytes/μl resulted with a sensitivity of 95.15% and a screening yield of 32.14%. Eleven false negative were obtained, but six of them showed low counts, and another was due to <em>Candida glabrata.</em> On the other hand, statistically significant variations were found as regards gender and origin of the patients. The cut-off of male samples was lower than female ones. However, it remained stable in the samples from Primary Care, and it decreased notably in those from the hospital. The negative predictive value always remained over 95%.</p></div><div><h3>Conclusion</h3><p>Automated flow cytometry can avoid the culture of 32% of samples, even after applying tight positive criteria. In the study hospital, it would have avoided the culture of 13,705 urine samples in the year 2016. These results could improve by combining cut-off points, gender, and origin of patients.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75254668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recomendaciones conjuntas EFLM-COLABIOCLI para la extracción de muestras de sangre venosa; armonizar desde la base 联合EFLM-COLABIOCLI对静脉血样本提取的建议;从基础上协调

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2019.02.001

Mercedes Ibarz , Rubén Gómez-Rioja

引用次数: 0

Valoración del uso del índice ictérico como parámetro para la gestión de la determinación de la bilirrubina total 使用黄疸指数作为管理总胆红素测定的参数的评价

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2018.12.002

Ramiro Antonio Torrado Carrión, Luz del Mar Rivas Chacón, Verónica Cámara Hernández, Marta de Paula Ruiz, Tomás Pascual Durán

{"title":"Valoración del uso del índice ictérico como parámetro para la gestión de la determinación de la bilirrubina total","authors":"Ramiro Antonio Torrado Carrión, Luz del Mar Rivas Chacón, Verónica Cámara Hernández, Marta de Paula Ruiz, Tomás Pascual Durán","doi":"10.1016/j.labcli.2018.12.002","DOIUrl":"10.1016/j.labcli.2018.12.002","url":null,"abstract":"<div><h3>Introduction</h3><p>As too many laboratory tests may not represent significant improvements, their efficient use should be considered. Bilirubin is a classical biochemical marker of hepatic alterations, and the icteric index is an indirect measure for the semi-quantitative determination of jaundice.</p></div><div><h3>Objectives</h3><p>To evaluate the use of the icteric index in the identification of patients with serum bilirubin concentrations with values higher or lower than 1.20mg/dL, as well as to assess the determination of bilirubin, and to evaluate the savings that the application of this algorithm would represent.</p></div><div><h3>Material and methods</h3><p>A retrospective study was performed to determine the relationship between icteric index and total bilirubin. A regression analysis was also performed. The diagnostic efficiency of the index was studied using a <em>Receiver Operating Characteristic</em> curve to determine the cut-off value that would allow to distinguish bilirubin values higher and lower than 1.20mg/dL. The sensitivity and specificity, positive predictive value and negative predictive value were also calculated.</p></div><div><h3>Results and discussion</h3><p>The statistical analysis showed a high correlation between both variables. The study of diagnostic efficacy showed that to use an icteric index equal to 2 as a cut-off point yields a high negative predictive value, sensitivity and specificity.</p></div><div><h3>Conclusions</h3><p>Icteric index values are well correlated with bilirubin, which allows filtering for hyperbilirubinaemia. Therefore, 89.72% of bilirubin requests would not be needed. With those samples with icteric index ≤ 1 subsequently being reported as<1.20mg/dL, thus representing a savings to the laboratory.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82005465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Recomendaciones en el proceso preanalítico del análisis de semen ii. Recomendación 2016 对精液ii分析前过程的建议。建议2016

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2017.12.001

María Cristina Sánchez-Pozo , Silvia Izquierdo Álvarez , Isabel Sánchez Prieto , María Isabel Jiménez García

引用次数: 0

Procedimiento para la interpretación de un cambio entre dos valores consecutivos de una magnitud biológica 解释生物量级两个连续值之间变化的程序

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2018.01.002

Raúl Rigo Bonnin, Ruth Cano Corres, Núria Alonso Nieva, María Jesús Andrés Otero, Francesca Canalias Reverter, Sara Esteve Poblador, Francisco Javier Gella Tomás, Bernardino González de la Presa, Rosa López Martínez, Inmaculada Pérez de Algaba Fuentes

{"title":"Procedimiento para la interpretación de un cambio entre dos valores consecutivos de una magnitud biológica","authors":"Raúl Rigo Bonnin, Ruth Cano Corres, Núria Alonso Nieva, María Jesús Andrés Otero, Francesca Canalias Reverter, Sara Esteve Poblador, Francisco Javier Gella Tomás, Bernardino González de la Presa, Rosa López Martínez, Inmaculada Pérez de Algaba Fuentes","doi":"10.1016/j.labcli.2018.01.002","DOIUrl":"10.1016/j.labcli.2018.01.002","url":null,"abstract":"<div><p>Biological reference intervals do not provide sufficient information for the interpretation of a change between two consecutive measured values of a biological quantity because, for the vast majority of quantities, the intra-individual biological variability is smaller than the inter-individual biological variability. Taking into account this situation, the laboratory could provide, in conjunction with the reference intervals, additional information to objectively estimate the significance of a change in the values of a biological quantity. In this sense, the most adequate way to interpret the change must be made on the basis of the uncertainty concept, since it allows taking into account all the possible sources of variation to which the measured values are subjected. This document, based on national and international guidelines, describes a procedure for the interpretation of a change between two consecutive values of a biological quantity, based on the study of the various sources of uncertainty that affect it.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75341418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seudohiperfosfatemia analítica como punto de partida en el diagnóstico de una disproteinemia 分析性假高磷血症作为诊断蛋白血症的起点

Revista del Laboratorio Clínico Pub Date : 2019-04-01 DOI: 10.1016/j.labcli.2018.03.003

Carlos Castillo-Pérez , Carmen Oana Minea , Marta Cebrián Ballesteros , Laura Rodriguez Alonso , Blanca Torrubia Dodero , Inmaculada Martín-Mérida

引用次数: 0