Redox Biochemistry and Chemistry最新文献_第4页

Special issue on “Peroxynitrite and Reactive Nitrogen Species” dedicated to the 25th anniversary of the nitric oxide Nobel Prize 诺贝尔一氧化氮奖25周年特刊《过氧亚硝酸盐与活性氮

Redox Biochemistry and Chemistry Pub Date : 2024-12-01 Epub Date: 2024-11-06 DOI: 10.1016/j.rbc.2024.100044

Ari Zeida, Jacek Zielonka, Madia Trujillo

引用次数: 0

Boronate-based bioactive compounds activated by peroxynitrite and hydrogen peroxide 过亚硝酸盐和过氧化氢激活的硼酸盐生物活性化合物

Redox Biochemistry and Chemistry Pub Date : 2024-12-01 Epub Date: 2024-08-14 DOI: 10.1016/j.rbc.2024.100040

Monika Rola , Jacek Zielonka , Renata Smulik-Izydorczyk , Jakub Pięta , Karolina Pierzchała , Adam Sikora , Radosław Michalski

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Dysfunctional copper homeostasis in Caenorhabditis elegans affects genomic and neuronal stability 秀丽隐杆线虫体内铜平衡失调会影响基因组和神经元的稳定性

Redox Biochemistry and Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-15 DOI: 10.1016/j.rbc.2024.100043

Ann-Kathrin Weishaupt , Anna Gremme , Torben Meiners , Vera Schwantes , Karsten Sarnow , Alicia Thiel , Tanja Schwerdtle , Michael Aschner , Heiko Hayen , Julia Bornhorst

{"title":"Dysfunctional copper homeostasis in Caenorhabditis elegans affects genomic and neuronal stability","authors":"Ann-Kathrin Weishaupt , Anna Gremme , Torben Meiners , Vera Schwantes , Karsten Sarnow , Alicia Thiel , Tanja Schwerdtle , Michael Aschner , Heiko Hayen , Julia Bornhorst","doi":"10.1016/j.rbc.2024.100043","DOIUrl":"10.1016/j.rbc.2024.100043","url":null,"abstract":"<div><div>While copper (Cu) is an essential trace element for biological systems due to its redox properties, excess levels may lead to adverse effects partly due to overproduction of reactive species. Thus, a tightly regulated Cu homeostasis is crucial for health. Cu dyshomeostasis and elevated labile Cu levels are associated with oxidative stress and neurodegenerative disorders, but the underlying mechanisms have yet to be fully characterized. Here, we used <em>Caenorhabditis elegans</em> loss-of-function mutants of the Cu chaperone ortholog atox-1 and the Cu binding protein ortholog ceruloplasmin to model Cu dyshomeostasis, as they display a shifted ratio of total Cu towards labile Cu. We applied highly selective and sensitive techniques to quantify metabolites associated to oxidative stress with focus on mitochondrial integrity, oxidative DNA damage and neurodegeneration all in the context of a disrupted Cu homeostasis. Our novel data reveal elevated oxidative stress, compromised mitochondria displaying reduced ATP levels and cardiolipin content. Cu dyshomeostasis further induced oxidative DNA damage and impaired DNA damage response as well as neurodegeneration characterized by behavior and neurotransmitter analysis. Our study underscores the essentiality of a tightly regulated Cu homeostasis as well as mitochondrial integrity for both genomic and neuronal stability.</div></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"10 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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NADPH oxidase 5: Where are we now and which way to proceed? NADPH 氧化酶 5：我们现在在哪里？

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 Epub Date: 2024-07-24 DOI: 10.1016/j.rbc.2024.100036

Gábor L. Petheő, Zsolt Szeles, Miklós Geiszt

{"title":"NADPH oxidase 5: Where are we now and which way to proceed?","authors":"Gábor L. Petheő, Zsolt Szeles, Miklós Geiszt","doi":"10.1016/j.rbc.2024.100036","DOIUrl":"10.1016/j.rbc.2024.100036","url":null,"abstract":"<div><p>Since the incorporation of mitochondria in early eukaryotes cells struggle to keep the deleterious effects of reactive oxygen species (ROS), mainly originating from the respiratory chain, at bay. Evolutionary adaptation to ROS burden went so far that by acting as messenger and effector molecules, ROS became important in maintaining homeostasis. The evolutionary success of this phenomenon is underscored by the arising of professional ROS-generating enzymes, namely the family of NADPH oxidases (NOXes). NOXes, by shaping ROS levels at different subcellular locations and in extracellular space, are involved in such fundamental functions as proliferation, differentiation, apoptosis, host defense, fertilization, and hormone biosynthesis. NOX5, being a calcium-regulated professional ROS source exerts its function at the crossroad of these two fundamental but potentially deleterious intracellular signaling pathways (i.e. Ca<sup>2+</sup> and ROS). The expression of NOX5 in the adult human body under unchallenged conditions is restricted to very few sites, among which the two major tissue groups are genital organs (mainly testis) and immune tissues (mainly spleen). In cases of increased cellular proliferation and protein synthesis (e.g., diverse tumors, cultured primary cells, or sites of tissue damage) the expression and activity of NOX5 is often upregulated in various tissues. This and the evolutionary conserved nature of NOX5 would imply a very fundamental role for this enzyme, but intriguingly the genomes of rodents essentially lack the NOX5 gene. The latter fact had been a major obstacle in determining the physiological roles of NOX5 in normal tissues until the very recent generation of a NOX5-deficient rabbit model.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000178/pdfft?md5=2687164805394d26bf3476b55ab647bc&pid=1-s2.0-S2773176624000178-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Comparing thiol and selenol reactivity towards peroxynitrite by computer simulation 通过计算机模拟比较硫醇和硒醇对过硫酸盐的反应性

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 Epub Date: 2024-07-23 DOI: 10.1016/j.rbc.2024.100035

Andresa Messias , Aníbal Rauber , Sofía Vuletich , Ari Zeida , Jonathan A. Semelak , Darío A. Estrin

{"title":"Comparing thiol and selenol reactivity towards peroxynitrite by computer simulation","authors":"Andresa Messias , Aníbal Rauber , Sofía Vuletich , Ari Zeida , Jonathan A. Semelak , Darío A. Estrin","doi":"10.1016/j.rbc.2024.100035","DOIUrl":"10.1016/j.rbc.2024.100035","url":null,"abstract":"<div><p>Peroxynitrite is a very reactive species implicated in a variety of pathophysiological cellular processes. Particularly, peroxynitrite-mediated oxidation of cellular thiol-containing compounds such as cysteine residues is a key process which has been extensively studied. Cysteine plays roles in many redox biochemistry pathways. In contrast, selenocysteine, the 21st amino acid, is only present in 25 human proteins. Investigating the molecular basis of selenocysteine's reactivity may provide insights into its unique role in these selenocysteine-containing proteins. The two-electron oxidation of thiols or selenols by peroxynitrite is a process that is carried out by the thiolate/selenate forms and peroxynitrous acid.</p><p>In this work, we shed light on the molecular basis of the differential reactivity of both species towards peroxynitrite by means of state-of-the-art computer simulations. We performed electronic structure calculations of the reaction in the methanethiolate and methaneselenolate model systems with peroxynitrous acid at different levels of theory using an implicit solvent scheme. In addition, we employed a multi-scale quantum mechanics/molecular mechanics approach for obtaining free energy profiles of these chemical reactions in aqueous solution, which enabled the comparison between the simulations and the available experimental data. Our results suggest that the larger reactivity observed in the selenocysteine case at physiological pH is mainly due to the lower pKa, which affords a larger fraction of the reactive anionic species in these conditions, and in a second place to a slightly enhanced intrinsic reactivity of the selenate form due to its larger nucleophilicity.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000166/pdfft?md5=b9af632f0685ee493c0ed788423b63f7&pid=1-s2.0-S2773176624000166-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The specificity of endogenous fatty acid nitration: only conjugated substrates support the in vivo formation of nitro-fatty acids 内源性脂肪酸硝化的特异性：只有共轭底物才支持体内硝基脂肪酸的形成

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 Epub Date: 2024-07-24 DOI: 10.1016/j.rbc.2024.100037

Nicole Colussi , Fei Chang , Francisco J. Schopfer

{"title":"The specificity of endogenous fatty acid nitration: only conjugated substrates support the in vivo formation of nitro-fatty acids","authors":"Nicole Colussi , Fei Chang , Francisco J. Schopfer","doi":"10.1016/j.rbc.2024.100037","DOIUrl":"10.1016/j.rbc.2024.100037","url":null,"abstract":"<div><p>Through multiple pathways, nitrogen dioxide (•NO<sub>2</sub>) is the main species involved in endogenous nitration reactions. Early studies in the field primarily explored tyrosine nitration, a dominant reaction in the field. It was later shown that lipids are also nitration targets and generate an array of reaction products. Conjugated fatty acids are the preferential substrates of lipid nitration in vivo, generating electrophilic nitro-fatty acids (NO<sub>2</sub>–FAs), which serve as pleiotropic signaling modulators. In contrast, exposure of bisallylic fatty acids, including linoleic, linolenic and arachidonic acid, to •NO<sub>2</sub> does not lead, under biological conditions, to the formation of nitrated species. This review focuses on the reaction mechanisms and products of lipid nitration and substrate specificity, focusing on the differential reactivity of conjugated dienes and bisallylic alkenes.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277317662400018X/pdfft?md5=96e201513036acdcd04293b5d3e95639&pid=1-s2.0-S277317662400018X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diffusion of peroxynitrite, its precursors, and derived reactive species, and the effect of cell membranes 过亚硝酸盐、其前体和衍生活性物种的扩散以及细胞膜的影响

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 Epub Date: 2024-07-05 DOI: 10.1016/j.rbc.2024.100033

Matías N. Möller, Ana Denicola

{"title":"Diffusion of peroxynitrite, its precursors, and derived reactive species, and the effect of cell membranes","authors":"Matías N. Möller, Ana Denicola","doi":"10.1016/j.rbc.2024.100033","DOIUrl":"10.1016/j.rbc.2024.100033","url":null,"abstract":"<div><p>Peroxynitrite is a powerful oxidant formed in vivo in sites where superoxide and nitric oxide coincide. Peroxynitrite is cytotoxic through oxidative modification of target biomolecules that can occur by direct or indirect reactions. Indirect reactions usually involve the generation of peroxynitrite-derived radicals that include nitrogen dioxide, hydroxyl radical, and carbonate radical. All these species have different behaviors in vivo, because of their intrinsic reactivity and how effectively they can be compartmentalized by cellular membranes. In this review, we analyze quantitative information on the estimated half-lives and the corresponding estimated diffusion distances of peroxynitrite, its precursors, and its derived reactive species in vivo. Furthermore, we discuss the permeability of cellular and synthetic lipid membranes to the different species and how effective compartmentalization is achieved for some of them, limiting the biological site of reactions.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000142/pdfft?md5=330518d6b68fea24fff7146d2022ce23&pid=1-s2.0-S2773176624000142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The chemistry of HNO-releasing compounds 释放 HNO 的化合物的化学性质

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 DOI: 10.1016/j.rbc.2024.100031

Renata Smulik-Izydorczyk , Jakub Pięta , Radosław Michalski , Monika Rola , Karol Kramkowski , Angelika Artelska , Jacek Zielonka , Adam Bartłomiej Sikora

引用次数: 0

Protein tyrosine nitration 蛋白质酪氨酸硝化

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 Epub Date: 2024-05-31 DOI: 10.1016/j.rbc.2024.100030

Harry Ischiropoulos

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Purine, nucleoside, and DNA nitration by peroxynitrite: Mechanistic considerations 过氧化亚硝酸对嘌呤、核苷和 DNA 的硝化作用：机理研究

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 Epub Date: 2024-05-15 DOI: 10.1016/j.rbc.2024.100028

Ana G. Sánchez, Deborah J. Keszenman, R. Daniel Peluffo

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