Redox Biochemistry and Chemistry最新文献

Editorial: Special issue celebrating the work of Prof. Christine C. Winterbourn

Redox Biochemistry and Chemistry Pub Date : 2024-12-01 DOI: 10.1016/j.rbc.2024.100045

Michael J. Davies (Joint Editor-in-Chief), Rafael Radi (Joint Editor-in-Chief)

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Special issue on “Peroxynitrite and Reactive Nitrogen Species” dedicated to the 25th anniversary of the nitric oxide Nobel Prize

Redox Biochemistry and Chemistry Pub Date : 2024-12-01 DOI: 10.1016/j.rbc.2024.100044

Ari Zeida, Jacek Zielonka, Madia Trujillo

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Dysfunctional copper homeostasis in Caenorhabditis elegans affects genomic and neuronal stability 秀丽隐杆线虫体内铜平衡失调会影响基因组和神经元的稳定性

Redox Biochemistry and Chemistry Pub Date : 2024-10-15 DOI: 10.1016/j.rbc.2024.100043

Ann-Kathrin Weishaupt , Anna Gremme , Torben Meiners , Vera Schwantes , Karsten Sarnow , Alicia Thiel , Tanja Schwerdtle , Michael Aschner , Heiko Hayen , Julia Bornhorst

{"title":"Dysfunctional copper homeostasis in Caenorhabditis elegans affects genomic and neuronal stability","authors":"Ann-Kathrin Weishaupt , Anna Gremme , Torben Meiners , Vera Schwantes , Karsten Sarnow , Alicia Thiel , Tanja Schwerdtle , Michael Aschner , Heiko Hayen , Julia Bornhorst","doi":"10.1016/j.rbc.2024.100043","DOIUrl":"10.1016/j.rbc.2024.100043","url":null,"abstract":"<div><div>While copper (Cu) is an essential trace element for biological systems due to its redox properties, excess levels may lead to adverse effects partly due to overproduction of reactive species. Thus, a tightly regulated Cu homeostasis is crucial for health. Cu dyshomeostasis and elevated labile Cu levels are associated with oxidative stress and neurodegenerative disorders, but the underlying mechanisms have yet to be fully characterized. Here, we used <em>Caenorhabditis elegans</em> loss-of-function mutants of the Cu chaperone ortholog atox-1 and the Cu binding protein ortholog ceruloplasmin to model Cu dyshomeostasis, as they display a shifted ratio of total Cu towards labile Cu. We applied highly selective and sensitive techniques to quantify metabolites associated to oxidative stress with focus on mitochondrial integrity, oxidative DNA damage and neurodegeneration all in the context of a disrupted Cu homeostasis. Our novel data reveal elevated oxidative stress, compromised mitochondria displaying reduced ATP levels and cardiolipin content. Cu dyshomeostasis further induced oxidative DNA damage and impaired DNA damage response as well as neurodegeneration characterized by behavior and neurotransmitter analysis. Our study underscores the essentiality of a tightly regulated Cu homeostasis as well as mitochondrial integrity for both genomic and neuronal stability.</div></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"10 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Perceptions of peroxynitrite reactivity – Then and now 对过氧化亚硝酸盐反应性的认识--过去和现在

Redox Biochemistry and Chemistry Pub Date : 2024-08-20 DOI: 10.1016/j.rbc.2024.100041

Sergei V. Lymar , James K. Hurst

{"title":"Perceptions of peroxynitrite reactivity – Then and now","authors":"Sergei V. Lymar , James K. Hurst","doi":"10.1016/j.rbc.2024.100041","DOIUrl":"10.1016/j.rbc.2024.100041","url":null,"abstract":"<div><div>Many chemical and biological reactions involving peroxynitrite<span><span><sup>3</sup></span></span> occur by unusual rate laws that are independent of the identity of the reacting partner. The true nature of these reactions and the identities of actual reactive species have been the subject of considerable debate ever since the notion that peroxynitrite is an important component of oxidative stress was first introduced in the early 1990s. We present herein a succinct historical review of this topic written from the perspective that intermediary inorganic free radicals are the causative agents in these reactions. This viewpoint provides a complete self-consistent rationalization of all verified data from multiple laboratories, whereas other explanations have been unable to do so. Recognition of the radical nature of peroxynitrite decomposition has also allowed a reassessment of the quantitative mechanism of CO<sub>2</sub>-catalyzed peroxynitrite decomposition. Detailed analyses indicate that the constant for rate-limiting formation of the putative reactive carbon dioxide adduct (<span><math><msup><mrow><msub><mtext>ONOOCO</mtext><mn>2</mn></msub></mrow><mo>−</mo></msup></math></span>)<span><span><sup>3</sup></span></span> is actually ∼20% less than previously recognized and CO<sub>2</sub> turnover numbers for catalysis (that is, the number of reaction cycles that CO<sub>2</sub> undergoes before being removed as bicarbonate) are relatively large and dependent upon the [CO<sub>2</sub>]/[ONOO<sup>−</sup>] ratio in the reaction environment.</div></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"10 ","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Boronate-based bioactive compounds activated by peroxynitrite and hydrogen peroxide 过亚硝酸盐和过氧化氢激活的硼酸盐生物活性化合物

Redox Biochemistry and Chemistry Pub Date : 2024-08-14 DOI: 10.1016/j.rbc.2024.100040

Monika Rola , Jacek Zielonka , Renata Smulik-Izydorczyk , Jakub Pięta , Karolina Pierzchała , Adam Sikora , Radosław Michalski

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Reaction of peroxynitrite with thiols, hydrogen sulfide and persulfides 过亚硝酸与硫醇、硫化氢和过硫化物的反应

Redox Biochemistry and Chemistry Pub Date : 2024-08-08 DOI: 10.1016/j.rbc.2024.100039

Madia Trujillo , Ernesto Cuevasanta , Lucía Turell , Dayana Benchoam , Gerardo Ferrer-Sueta , Ari Zeida , Celia Quijano , Sebastián Carballal , Rafael Radi , Beatriz Alvarez

{"title":"Reaction of peroxynitrite with thiols, hydrogen sulfide and persulfides","authors":"Madia Trujillo , Ernesto Cuevasanta , Lucía Turell , Dayana Benchoam , Gerardo Ferrer-Sueta , Ari Zeida , Celia Quijano , Sebastián Carballal , Rafael Radi , Beatriz Alvarez","doi":"10.1016/j.rbc.2024.100039","DOIUrl":"10.1016/j.rbc.2024.100039","url":null,"abstract":"<div><p>Three decades of research on the biochemistry of peroxynitrite (ONOOH/ONOO<sup>−</sup>) have established that this stealthy oxidant is formed in biological systems, and that its main targets are carbon dioxide (CO<sub>2</sub>), metalloproteins and thiols (RSH). Peroxynitrous acid reacts directly with thiols (precisely, with thiolates, RS<sup>−</sup>), forming sulfenic acids (RSOH). In addition, the free radicals derived from peroxynitrite, mainly carbonate radical anion (<span><math><msup><msub><mi>CO</mi><mn>3</mn></msub><mrow><mo>•</mo><mo>−</mo></mrow></msup></math></span>) and nitrogen dioxide (<span><math><msup><msub><mi>NO</mi><mn>2</mn></msub><mrow><mo>•</mo></mrow></msup></math></span>) formed from the reaction of peroxynitrite anion with CO<sub>2</sub>, oxidize thiols to thiyl radicals (RS<sup>•</sup>). These two pathways are under kinetic competition. The primary products of thiol oxidation can follow different decay routes; sulfenic acids usually react with other thiols forming disulfides, while thiyl radicals can react with oxygen, with other thiols and with other reductants such as ascorbic acid. Peroxynitrite is also able to oxidize hydrogen sulfide (H<sub>2</sub>S/HS<sup>−</sup>) and persulfides (RSSH/RSS<sup>−</sup>). Among the different biological thiols, peroxiredoxins stand out as main peroxynitrite reductases due to their very high rate constants of reaction with peroxynitrite together with their abundance. Rooted in kinetic concepts, evidence is emerging for the role of peroxiredoxins in peroxynitrite detoxification, with potential implications in diseases in which peroxynitrite is involved.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"10 ","pages":"Article 100039"},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000208/pdfft?md5=d3ba6f796dbd6aef9cd2c5262abce81f&pid=1-s2.0-S2773176624000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Peroxynitrite: A tale of two radicals 亚硝酸过氧化物两个自由基的故事

Redox Biochemistry and Chemistry Pub Date : 2024-08-03 DOI: 10.1016/j.rbc.2024.100038

Patricia L. Bounds , Willem H. Koppenol

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NADPH oxidase 5: Where are we now and which way to proceed? NADPH 氧化酶 5：我们现在在哪里？

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 DOI: 10.1016/j.rbc.2024.100036

Gábor L. Petheő, Zsolt Szeles, Miklós Geiszt

{"title":"NADPH oxidase 5: Where are we now and which way to proceed?","authors":"Gábor L. Petheő, Zsolt Szeles, Miklós Geiszt","doi":"10.1016/j.rbc.2024.100036","DOIUrl":"10.1016/j.rbc.2024.100036","url":null,"abstract":"<div><p>Since the incorporation of mitochondria in early eukaryotes cells struggle to keep the deleterious effects of reactive oxygen species (ROS), mainly originating from the respiratory chain, at bay. Evolutionary adaptation to ROS burden went so far that by acting as messenger and effector molecules, ROS became important in maintaining homeostasis. The evolutionary success of this phenomenon is underscored by the arising of professional ROS-generating enzymes, namely the family of NADPH oxidases (NOXes). NOXes, by shaping ROS levels at different subcellular locations and in extracellular space, are involved in such fundamental functions as proliferation, differentiation, apoptosis, host defense, fertilization, and hormone biosynthesis. NOX5, being a calcium-regulated professional ROS source exerts its function at the crossroad of these two fundamental but potentially deleterious intracellular signaling pathways (i.e. Ca<sup>2+</sup> and ROS). The expression of NOX5 in the adult human body under unchallenged conditions is restricted to very few sites, among which the two major tissue groups are genital organs (mainly testis) and immune tissues (mainly spleen). In cases of increased cellular proliferation and protein synthesis (e.g., diverse tumors, cultured primary cells, or sites of tissue damage) the expression and activity of NOX5 is often upregulated in various tissues. This and the evolutionary conserved nature of NOX5 would imply a very fundamental role for this enzyme, but intriguingly the genomes of rodents essentially lack the NOX5 gene. The latter fact had been a major obstacle in determining the physiological roles of NOX5 in normal tissues until the very recent generation of a NOX5-deficient rabbit model.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000178/pdfft?md5=2687164805394d26bf3476b55ab647bc&pid=1-s2.0-S2773176624000178-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Comparing thiol and selenol reactivity towards peroxynitrite by computer simulation 通过计算机模拟比较硫醇和硒醇对过硫酸盐的反应性

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 DOI: 10.1016/j.rbc.2024.100035

Andresa Messias , Aníbal Rauber , Sofía Vuletich , Ari Zeida , Jonathan A. Semelak , Darío A. Estrin

{"title":"Comparing thiol and selenol reactivity towards peroxynitrite by computer simulation","authors":"Andresa Messias , Aníbal Rauber , Sofía Vuletich , Ari Zeida , Jonathan A. Semelak , Darío A. Estrin","doi":"10.1016/j.rbc.2024.100035","DOIUrl":"10.1016/j.rbc.2024.100035","url":null,"abstract":"<div><p>Peroxynitrite is a very reactive species implicated in a variety of pathophysiological cellular processes. Particularly, peroxynitrite-mediated oxidation of cellular thiol-containing compounds such as cysteine residues is a key process which has been extensively studied. Cysteine plays roles in many redox biochemistry pathways. In contrast, selenocysteine, the 21st amino acid, is only present in 25 human proteins. Investigating the molecular basis of selenocysteine's reactivity may provide insights into its unique role in these selenocysteine-containing proteins. The two-electron oxidation of thiols or selenols by peroxynitrite is a process that is carried out by the thiolate/selenate forms and peroxynitrous acid.</p><p>In this work, we shed light on the molecular basis of the differential reactivity of both species towards peroxynitrite by means of state-of-the-art computer simulations. We performed electronic structure calculations of the reaction in the methanethiolate and methaneselenolate model systems with peroxynitrous acid at different levels of theory using an implicit solvent scheme. In addition, we employed a multi-scale quantum mechanics/molecular mechanics approach for obtaining free energy profiles of these chemical reactions in aqueous solution, which enabled the comparison between the simulations and the available experimental data. Our results suggest that the larger reactivity observed in the selenocysteine case at physiological pH is mainly due to the lower pKa, which affords a larger fraction of the reactive anionic species in these conditions, and in a second place to a slightly enhanced intrinsic reactivity of the selenate form due to its larger nucleophilicity.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000166/pdfft?md5=b9af632f0685ee493c0ed788423b63f7&pid=1-s2.0-S2773176624000166-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The specificity of endogenous fatty acid nitration: only conjugated substrates support the in vivo formation of nitro-fatty acids 内源性脂肪酸硝化的特异性：只有共轭底物才支持体内硝基脂肪酸的形成

Redox Biochemistry and Chemistry Pub Date : 2024-08-01 DOI: 10.1016/j.rbc.2024.100037

Nicole Colussi , Fei Chang , Francisco J. Schopfer

{"title":"The specificity of endogenous fatty acid nitration: only conjugated substrates support the in vivo formation of nitro-fatty acids","authors":"Nicole Colussi , Fei Chang , Francisco J. Schopfer","doi":"10.1016/j.rbc.2024.100037","DOIUrl":"10.1016/j.rbc.2024.100037","url":null,"abstract":"<div><p>Through multiple pathways, nitrogen dioxide (•NO<sub>2</sub>) is the main species involved in endogenous nitration reactions. Early studies in the field primarily explored tyrosine nitration, a dominant reaction in the field. It was later shown that lipids are also nitration targets and generate an array of reaction products. Conjugated fatty acids are the preferential substrates of lipid nitration in vivo, generating electrophilic nitro-fatty acids (NO<sub>2</sub>–FAs), which serve as pleiotropic signaling modulators. In contrast, exposure of bisallylic fatty acids, including linoleic, linolenic and arachidonic acid, to •NO<sub>2</sub> does not lead, under biological conditions, to the formation of nitrated species. This review focuses on the reaction mechanisms and products of lipid nitration and substrate specificity, focusing on the differential reactivity of conjugated dienes and bisallylic alkenes.</p></div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277317662400018X/pdfft?md5=96e201513036acdcd04293b5d3e95639&pid=1-s2.0-S277317662400018X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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