Redox Biochemistry and Chemistry最新文献_第2页

Small molecule probes for peroxynitrite detection 用于检测过亚硝酸盐的小分子探针

Redox Biochemistry and Chemistry Pub Date : 2024-07-26 DOI: 10.1016/j.rbc.2024.100034

Aleksandra Grzelakowska , Balaraman Kalyanaraman , Jacek Zielonka

{"title":"Small molecule probes for peroxynitrite detection","authors":"Aleksandra Grzelakowska , Balaraman Kalyanaraman , Jacek Zielonka","doi":"10.1016/j.rbc.2024.100034","DOIUrl":"10.1016/j.rbc.2024.100034","url":null,"abstract":"<div>Peroxynitrite (ONOO−/ONOOH) is a short-lived but highly reactive species that is formed in the diffusion-controlled reaction between nitric oxide and the superoxide radical anion. It can oxidize certain biomolecules and has been considered as a key cellular oxidant formed under various pathophysiological conditions. It is crucial to selectively detect and quantify ONOO− to determine its role in biological processes. In this review, we discuss various approaches used to detect ONOO− in cell-free and cellular systems with the major emphasis on small-molecule chemical probes. We review the chemical principles and mechanisms responsible for the formation of the detectable products, and plausible limitations of the probes. We recommend the use of boronate-based chemical probes for ONOO−, as they react directly and rapidly with ONOO−, they produce minor but ONOO−‒specific products, and the reaction kinetics and mechanism have been rigorously characterized. Specific experimental approaches and protocols for the detection of ONOO− in cell-free, cellular, and in vivo systems using boronate-based molecular probes are provided (as shown in Boxes 1-6).</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"10 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000154/pdfft?md5=a4723535d75e5476ac6136a1e8cdeac7&pid=1-s2.0-S2773176624000154-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141847473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diffusion of peroxynitrite, its precursors, and derived reactive species, and the effect of cell membranes 过亚硝酸盐、其前体和衍生活性物种的扩散以及细胞膜的影响

Redox Biochemistry and Chemistry Pub Date : 2024-07-05 DOI: 10.1016/j.rbc.2024.100033

Matías N. Möller, Ana Denicola

{"title":"Diffusion of peroxynitrite, its precursors, and derived reactive species, and the effect of cell membranes","authors":"Matías N. Möller, Ana Denicola","doi":"10.1016/j.rbc.2024.100033","DOIUrl":"10.1016/j.rbc.2024.100033","url":null,"abstract":"<div>Peroxynitrite is a powerful oxidant formed in vivo in sites where superoxide and nitric oxide coincide. Peroxynitrite is cytotoxic through oxidative modification of target biomolecules that can occur by direct or indirect reactions. Indirect reactions usually involve the generation of peroxynitrite-derived radicals that include nitrogen dioxide, hydroxyl radical, and carbonate radical. All these species have different behaviors in vivo, because of their intrinsic reactivity and how effectively they can be compartmentalized by cellular membranes. In this review, we analyze quantitative information on the estimated half-lives and the corresponding estimated diffusion distances of peroxynitrite, its precursors, and its derived reactive species in vivo. Furthermore, we discuss the permeability of cellular and synthetic lipid membranes to the different species and how effective compartmentalization is achieved for some of them, limiting the biological site of reactions.</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"9 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000142/pdfft?md5=330518d6b68fea24fff7146d2022ce23&pid=1-s2.0-S2773176624000142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chemistry of HNO-releasing compounds 释放 HNO 的化合物的化学性质

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 DOI: 10.1016/j.rbc.2024.100031

Renata Smulik-Izydorczyk , Jakub Pięta , Radosław Michalski , Monika Rola , Karol Kramkowski , Angelika Artelska , Jacek Zielonka , Adam Bartłomiej Sikora

引用次数: 0

Protein tyrosine nitration 蛋白质酪氨酸硝化

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 DOI: 10.1016/j.rbc.2024.100030

Harry Ischiropoulos

引用次数: 0

Role of sulfane sulfur species in elemental tellurium nanorod formation in mammalian cells 哺乳动物细胞中碲元素纳米棒形成过程中的硫磺物种作用

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 DOI: 10.1016/j.rbc.2024.100029

Yu-ki Tanaka , Hiroki Yanagi , Ayako Shiokawa , Akihiro Matsunaga , Mari Shimura , Satoshi Matsuyama , Yasunori Fukumoto , Noriyuki Suzuki , Yasumitsu Ogra

{"title":"Role of sulfane sulfur species in elemental tellurium nanorod formation in mammalian cells","authors":"Yu-ki Tanaka , Hiroki Yanagi , Ayako Shiokawa , Akihiro Matsunaga , Mari Shimura , Satoshi Matsuyama , Yasunori Fukumoto , Noriyuki Suzuki , Yasumitsu Ogra","doi":"10.1016/j.rbc.2024.100029","DOIUrl":"https://doi.org/10.1016/j.rbc.2024.100029","url":null,"abstract":"<div>Tellurium (Te) is an industrially useful element but its oxyanions, such as tellurite and tellurate, are naturally occurring chemical forms that can become a potential source of toxicity to humans and animals. As a means of mitigating the toxicity of Te oxyanions, the formation of less toxic zero-valent elemental Te (Te0) nanostructures has been observed in various species including bacteria, fungi, green algae, and higher plants. In this study, we investigated the formation of Te0 nanorods in human hepatoma HepG2 cells. We detected electron-dense Te nanorods in lysosomes after exposure to potassium tellurite. The amount of Te nanorods in the cells gradually increased with the exposure period. Interestingly, the amount of Te in the insoluble fraction of the culture supernatant was approximately 10 times higher than that in HepG2 cells, suggesting that extracellular reducing agents originating from HepG2 cells transformed tetravalent Te (TeO32−) into Te0 in the culture medium. As an extracellular reducing agent, sulfane sulfur species were considered responsible for the reduction of Te(IV). Then, by inhibiting cystathionine γ-lyase with propargylglycine (PPG), we were able to reduce the amount of sulfane sulfur species generated in the cells. In the presence of PPG, the amount of insoluble Te in the culture supernatant, which was possibly composed of Te0 nanorods, was significantly decreased. The results suggest that sulfane sulfur species are involved in the formation of Te0 nanorods from tellurite in mammalian cells and play a critical role in the amelioration of Te oxyanion toxicity.</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"8 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000105/pdfft?md5=361e503f29b34dd81191f837af98e0d8&pid=1-s2.0-S2773176624000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141244416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein nitration in the artery wall: A contributor to cardiovascular disease? 动脉壁中的蛋白质硝化：心血管疾病的诱因？

Redox Biochemistry and Chemistry Pub Date : 2024-06-01 DOI: 10.1016/j.rbc.2024.100032

Michael J. Davies

{"title":"Protein nitration in the artery wall: A contributor to cardiovascular disease?","authors":"Michael J. Davies","doi":"10.1016/j.rbc.2024.100032","DOIUrl":"10.1016/j.rbc.2024.100032","url":null,"abstract":"<div>Nitration is a well-established post-translational modification of selected free amino acids, as well as proteins, lipids and nucleic acids. Considerable evidence is now available for the formation of long-lived species containing an added –NO2 function on the aromatic rings of tyrosine (Tyr) and tryptophan (Trp) residues (both free and on proteins), to purine nucleobases (and particularly guanine), and to unsaturated lipids within biological systems. Multiple potential mechanisms that give rise to these nitrated species have been identified including reactions of the potent oxidant and nitrating species peroxynitrous acid/peroxynitrite (ONOOH/ONOO−) and via oxidative reactions of heme proteins/enzymes (e.g. peroxidases) with the biologically-relevant anion nitrite (NO2−). •NO2 is likely to be a key intermediate, though involvement of HNO2, NO2+ and NO2Cl has also been proposed. The resulting nitrated products have been widely employed as qualitative or quantitative biomarkers of nitration events in vitro and in vivo. Increasing evidence suggests that at least some of these products are not benign species, with evidence for pro-inflammatory actions. In this article the mechanisms and role of nitration, and particularly that on proteins within the artery wall, in cardiovascular disease is discussed, together with emerging data suggesting that low levels of nitration occur within biological systems in the absence of added oxidants. Both stimulated and endogenous nitration may play a role in modulating cell signaling, alter the structure and function of both cellular- and extracellular proteins, and contribute to various inflammatory pathologies, including atherosclerosis.</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"8 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000130/pdfft?md5=145e5634e1cd27124e29854f3c750e6b&pid=1-s2.0-S2773176624000130-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purine, nucleoside, and DNA nitration by peroxynitrite: Mechanistic considerations 过氧化亚硝酸对嘌呤、核苷和 DNA 的硝化作用：机理研究

Redox Biochemistry and Chemistry Pub Date : 2024-05-15 DOI: 10.1016/j.rbc.2024.100028

Ana G. Sánchez, Deborah J. Keszenman, R. Daniel Peluffo

引用次数: 0

Nitro-fatty acid signaling: Therapeutic potential in inflammatory diseases 硝基脂肪酸信号传导：炎症性疾病的治疗潜力

Redox Biochemistry and Chemistry Pub Date : 2024-05-14 DOI: 10.1016/j.rbc.2024.100027

Homero Rubbo, Andrés Trostchansky

引用次数: 0

The chemical biology of dinitrogen trioxide 三氧化二氮的化学生物学

Redox Biochemistry and Chemistry Pub Date : 2024-05-09 DOI: 10.1016/j.rbc.2024.100026

Matías N. Möller , Darío A. Vitturi

{"title":"The chemical biology of dinitrogen trioxide","authors":"Matías N. Möller , Darío A. Vitturi","doi":"10.1016/j.rbc.2024.100026","DOIUrl":"https://doi.org/10.1016/j.rbc.2024.100026","url":null,"abstract":"<div>Dinitrogen trioxide (N2O3) mediates low-molecular weight and protein S- and N-nitrosation, with recent reports suggesting a role in the formation of nitrating intermediates as well as in nitrite-dependent hypoxic vasodilatation. However, the reactivity of N2O3 in biological systems results in an extremely short half-life that renders this molecule essentially undetectable by currently available technologies. As a result, evidence for in vivo N2O3 formation derives from the detection of nitrosated products as well as from in vitro kinetic determinations, isotopic labeling studies, and spectroscopic analyses. This review will discuss mechanisms of N2O3 formation, reactivity and decomposition, as well as address the role of sub-cellular localization as a key determinant of its actions. Finally, evidence will be discussed supporting different roles for N2O3 as a biologically relevant signaling molecule.</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"8 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000075/pdfft?md5=a6dcbb5b09dfa3f7a85e72ede30c0872&pid=1-s2.0-S2773176624000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct fitting improves the accuracy of the horse radish peroxidase competition assay for peroxidase activity 直接拟合提高了萝卜过氧化物酶竞争测定法测定过氧化物酶活性的准确性

Redox Biochemistry and Chemistry Pub Date : 2024-04-26 DOI: 10.1016/j.rbc.2024.100025

Christopher J. Barry , Ché S. Pillay , Johann M. Rohwer

{"title":"Direct fitting improves the accuracy of the horse radish peroxidase competition assay for peroxidase activity","authors":"Christopher J. Barry , Ché S. Pillay , Johann M. Rohwer","doi":"10.1016/j.rbc.2024.100025","DOIUrl":"https://doi.org/10.1016/j.rbc.2024.100025","url":null,"abstract":"<div>The peroxiredoxins are an important antioxidant protein family and their ability to neutralise oxidants is regularly investigated using horse radish peroxidase in a competition assay system. In this method, the rate constant of a peroxiredoxin is calculated from the fractional inhibition of horse radish peroxidase activity caused by competition with the peroxiredoxin for an oxidant substrate. We developed a model capable of simulating this assay and, using this model, demonstrate that the fractional inhibition calculation significantly and systematically mis-estimates the rate constant under fairly common conditions. We go on to develop a method for fitting simulated assay time-courses to experimental data directly, which significantly outperforms the fractional inhibition method yielding more accurate results. Based on our findings, we recommend using the direct fitting approach to determine peroxidase rate constants from horseradish peroxidase experiments.</div>","PeriodicalId":101065,"journal":{"name":"Redox Biochemistry and Chemistry","volume":"8 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773176624000063/pdfft?md5=d1ae63fed576a6bb22d227fdefdd47ca&pid=1-s2.0-S2773176624000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140823817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0