中华检验医学杂志最新文献

{"title":"Evaluation of biochemical and immunological assembly line in emergency test","authors":"X. Ji, Yan Dai","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.01.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.01.002","url":null,"abstract":"Objective \u0000To explore the application value of biochemical and immunological assembly line in emergency test by comparatively analyzing the changes in sample test process and turnaround time (TAT) in emergency test before and after installation of the assembly line. \u0000 \u0000 \u0000Methods \u0000A retrospective study was performed with the run data of the emergency biochemical and immunodetection project in Department of Clinical Laboratory in Tongji Hospital Affiliated to Tongji University from July to September in 2017 and from July to September in 2018. (1)The changes in sample circulation mode and test process were compared in emergency test before and after installation of the assembly line.(2)The changes in TAT of sample test and distribution of time interval in emergency laboratory report were statistically analyzed before and after installation.(3)The number of samples tested between instruments and occurrence of instrument failure were statistically compared before and after installation. (4)Biosafety risk assessment was performed on the sample test process before and after installation to analyze the effects of the assembly line installation on biosafety control in emergency test. \u0000 \u0000 \u0000Results \u0000(1) By applying the assembly line, the sample test process was well controlled and the workload for laboratorians was eased greatly through reduction of manual work.(2) Report TAT was remarkably shortened after installation compared to that before installation; the TAT of dry biochemistry and myocardial zymogram report in emergency test was shorted from 39 min and 48 min to 34 min and 42 min respectively, with a reduction of 12.82% an 12.50% compared to those before installation; the TAT of dry biochemistry and myocardial zymogram report in inpatient emergency was shorted from 37 min and 43 min to 29 min and 35 min respectively, with a reduction of 21.61% an 18.60% compared to those before installation; distribution of report TAT was comparatively analyzed, the percentage of reports in 0-30 min and 31-45 min increased remarkably compared to that before installation, the percentage of TAT of dry biochemistry report falling into 0-45 min increased to 74.58% and 85.66% after installation from 57.91% and 78.28% before installation, respectively in emergency and inpatient emergency. (3)For the model VITROS5600, the percentage of samples tested changed from 30.29% and 69.71% before installation to 47.63% and 52.37% after installation, respectively; for the model DXI800, the percentage of samples tested changed from 28.77% and 71.23% before installation to 53.49% and 46.51% after installation, respectively. After installation, there was a tendency of balance in the number of samples tested between instruments. Instrument failure was significantly reduced: for the model VITROS5600, the frequency of instrument failure decreased from 3.5 times a month/instrument before installation to 2 times a month/instrument after installation on average; for the model DXI800, the freq","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"467 1","pages":"28-35"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91086327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a candidate reference method for serum calcium determination based on inductively coupled plasma mass spectrometry","authors":"Xiaoyu Fan, Y. Ju","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.006","url":null,"abstract":"Objective \u0000To establish a candidate reference method for serum calcium determination based on inductively coupled plasma mass spectrometry(ICP-MS). \u0000 \u0000 \u0000Methods \u0000Establishment of methodology: serum samples collected from the hospital were diluted 100 times by 0.3% ultrapure nitric acid. Calcium standard solutions with different concentrations were added to serum samples matrix solutions. Serum calcium concentration was calculated according to the standard addition method using germanium (Ge) as internal standard. The linearity, precision, trueness of the performance of the established candidate reference method were evaluated, and a comparison with the reference method was made. \u0000 \u0000 \u0000Results \u0000The linearity was good in serum calcium concentrations from 0.000 mmol/L to 20.400 mmol/L (the concentration after dilution from 0.000 mmol/L to 0.204 mmol/L) (R2>0.999 9);the within-run precision was from 0.22% to 0.47%, and the between-run precision was from 0.64% to 0.77%; the total precision was from 0.98% to 1.09%. The determination results of 3 concentrations of SRM 956d were all in the uncertainty range required bythe certificate. The biases were -0.16%, 0.04% and 0.23%, respectively. The established candidate reference method participated in and passed the 2017 External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine (RELA) comparison. The results of the established candidate reference method were consistent well with those of the reference method in the Joint Committee for Traceability in Laboratory Medicine (JCTLM) list. There was a good correlation between the established candidate reference method and the clinical routine electrode method. \u0000 \u0000 \u0000Conclusions \u0000The established candidate reference method for serum calcium determination based on ICP-MS is precise, accurate, with wide range of linearity.It is expected to play a role in tracing the quantities of serum calcium in clinical laboratories. \u0000 \u0000 \u0000Key words: \u0000Calcium; Mass spectrometry; Reference standards","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"24 1","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83949736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of thrombus molecular markers in patients with malignant tumor","authors":"Yangyang Zheng, Hairun Yan, Qi Li, H-G Ji, Zheng-bing Yang, Qinzhen Zhang","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.009","url":null,"abstract":"Objective \u0000To explore the early diagnostic value of thrombus molecular markers in thrombosis ofpatients with malignant tumors and to evaluate their risk factors. \u0000 \u0000 \u0000Methods \u0000Diagnostic research.A total of 1366 patients (including lung cancer, breast cancer and colorectal cancer,) were randomly selected in the Red Flag Hospital of Mudanjiang Medical College and Mudanjiang Cancer Hospitalfrom September 2009 to February 1919. Among them, 562 were males and 804 were females with average age (59.45±15.10) years old. The control group consisted of 70healthy donors (35 males and 35 females, with an average age of (49.60±19.12) years old), including 69 cases of venous thrombosis (thrombotic group, 32 males and37 females, with an average age of (61.20±15.71) years old).Chemoluminescent enzyme immunoassay was used to detect thromboregulatory proteins(TM), thrombin-antithrombin complexes(TAT), tissue plasminogen activators/inhibitors -1 complexes(t-PAIC), plasminase-anti-fibrinolysis complexes(PIC) in venous plasma. According to the sensitivity and specificity of each marker, the receiver′s work characteristic curve was drawn to evaluate its diagnostic performance. Cox regression analysis was used for single-factor and multi-factor risk analysis. \u0000 \u0000 \u0000Results \u0000The incidence of venous thromboembolism(VTE) in patients with different types of malignant tumors was statistically significant, with lung cancer being the highest, followed by colorectal cancer and breast cancer(P<0.05). The levels of TM, TAT, t-PAIC and PIC were significantly higher in the lung, breast and colorectal thrombosis group than in the control group. The differences were statistically significant(all P<0.05). The optimal cut-off level for TM is 10.57 IU/ml(sensitivity 50.30%, specificity 75.50%, AUC=0.671), and the optimal cut-off level for TAT is 4.16 ng/ml(sensitivity is 80.30%, specificity is 62.80%, AUC=0.757).The optimal truncation level for t-PAIC is 11.44 ng/ml(sensitivity 52.50%, specificity 84.00%, AUC=0.682), and the optimal truncation level for PIC is 1.18μg/ml(sensitivity 67.20%, specificity is 79.50%, AUC=0.790). The combined detection of the four molecular markers has the best sensitivity and diagnostic performance(86.90%, AUC=0.807). Age, stage, metastasis, surgery, tumor diameter, and PIC levels are independent factors that affect the occurrence of VTE in malignant tumors (all P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Different types of malignant tumors have different rates of thrombosis. The combined detection ofTM, TAT, t-PAIC and PIC have the best diagnostic performance, and can be used as a new early diagnosis method for VTE in malignant tumors. Age, stage, metastasis, surgery, and tumor diameter are risk factors for VTE in malignant tumors. PIC levels can be used as a reliable markerfor the risk of VTE in patients with malignant tumors within 6 months. \u0000 \u0000 \u0000Key words: \u0000Neoplasms; Venous thromboembolism; Biomarkers","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"281 1","pages":"78-84"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76803434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of Shanghai glycohemoglobin harmonization program","authors":"Beili Wang, Yiwen Zhou, Jiong Wu, Wenqi Shao, Wen Xu, W. Guo","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.003","url":null,"abstract":"Objective \u0000To review the results of inter-laboratory comparisons in Shanghai glycohemoglobin harmonization program from 2010 to 2018, and to analyze the evolution of quality levels of HbA1c determination, so as to provide the reference for improving the HbA1c determination quality in China. \u0000 \u0000 \u0000Methods \u0000Retrospective analysis. The comparison data of Shanghai Glycohemoglobin Harmonization Program from 2010 to 2018 was collected. And the change trend was analyzed about hospital and determination method distribution. The judgment criteria, quarterly and annual pass rate, bias and coefficient of variation of the results of the inter-laboratory comparison were analyzed retrospectively, and the results were compared with the results of External Quality Assessment Programme carried out by the National Center for Clinical Laboratories, Shanghai Center for Clinical Laboratories and College of American Pathologists (CAP). The data in the first quarter of 2019 was collected and the imprecision, bias and sigma were calculated, which were drew in the evaluation model of sigma combined with biomedical variation parameters. \u0000 \u0000 \u0000Results \u0000The number of participating laboratories increased from 9 in Shanghai to 192 in the whole country, with an average annual growth rate of 76.6%. The quarterly comparison criteria improved from ±8% to ±6% and the passing rate of participating laboratories increased from 39.1% to nearly 90%. The maximum CV of each instrument among laboratories decreased from 14.3% to 4.8%. In the first quarter of 2019, nearly 60% of the laboratories met 6σcriteria and more than 95% of the laboratories met the \"standard criteria\" in the model of biological variation parameters. \u0000 \u0000 \u0000Conclusion \u0000Shanghai Glycohemoglobin harmonization program has improved the harmonization of HbA1c test results among the participating laboratories. \u0000 \u0000 \u0000Key words: \u0000Diabetes mellitus; Glycated hemoglobin A; Reproducibility of results","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"255 1","pages":"36-43"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90601980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commutability assessment of 16 processed materials for 17-hydroxyprogesterone based on two approaches in China","authors":"Qichen Long, Tianjiao Zhang, Ying Yan, Weiyan Zhou, Xin Li, Shuijun Li, Haijian Zhao, Wenxiang Chen, Chuanbao Zhang","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.004","url":null,"abstract":"Objective \u0000The aim of this study is to evaluate the commutability of 16 processed materials for 17-hydroxyprogesterone by using 2 commutability assessment approaches. \u0000 \u0000 \u0000Methods \u000052 serum specimens were collected in Clinical Laboratory Department of Beijing Hospital from February 2018 to June 2019. According to the report of the Clinical and Laboratory Standards Institute (EP14-A3) document and the recommendations of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) working group on commutabilityassessment, serum 17-hydroxyprogesterone isotope diluent chromatogram tandem mass spectrometry (ID-LC/MS/MS) was used for comparison. Three clinical routine analysis systems (1 radioimmunoassay, 2 LC/MS analysis methods) were used to determine the concentration of 17-hydroxyprogesterone in 52 human serum samples and 16 processed materialsfor commutabilityassessment. \u0000 \u0000 \u0000Results \u0000Combined with the results of the two commutability assessment, all accuracy verification materials and national steroid hormone standards showed good commutability in the LC/MS analysis system, and 6/9 EQA materials showed commutability in the three routine analysis systems.All materials showed good commutability in the LC/MS analysis system of bias difference method. \u0000 \u0000 \u0000Conclusions \u0000The two kinds of commutability assessment results are different. Bias difference method has more clinical value, but it has certain application limitations. The use of fresh frozen human serum as a quality assessment materialfor serum 17-hydroxyprogesterone is meets the commutability requirement. \u0000 \u0000 \u0000Key words: \u000017-α-Hydroxyprogesterone; Quality control; Chromatography, liquid; Ttandem mass spectrometry","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"40 1","pages":"44-50"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86345710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of serum cytokine level in the evaluation of the efficacy of tocilizumab for the treatment of systemic-onset juvenile idiopathic arthritis","authors":"G. Su, XiaoLan Huang, Zhi-xuan Zhou, Feng-qi Wu, J. Lai","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.01.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.01.010","url":null,"abstract":"Objective \u0000To evaluate the value of serum cytokine level in the efficacy of tocilizumab for the treatment of systemic-onset juvenile idiopathic arthritis. \u0000 \u0000 \u0000Methods \u000030 cases with SoJIA hospitalized in Capital Institute of Paediatrics from June 2016 to October 2018 were treated with Interleukin-6 receptor antagonist(tocilizumab) injection. Among them, 20 were males(67%) and 10 were females(33%). The age at diagnosis was between 0.84 to 13years. Whiteblood cell, C-reactive protein, erythrocyte sedimentation rate, serum interleukin(IL-6, IL-2R, IL-8, IL-10, IL-1β) and tumor necrosis factor-alpha levels were observed before treatment, after the 2nd week, after the 6th week and after the 22nd week.Mann-Whitney nonparametric test and Chi-square test were used to analyze the data of cytokines pre and after-treatment. \u0000 \u0000 \u0000Results \u0000All of the 30 cases had fever before medication. The fever disappeared in 28 cases after using tocilizumab. One case stopped using tocilizumab because of allergic reaction and one case stopped because of poor efficacy. Among 28 cases with normal body temperature after medication, the arthritis and rash manifestations were significantly improved. WBC, AESR and CRP were all lower than those before medication. Within these 28 cases, the serum IL-6 level was168.50(67.40-589.25) pg/mL pre-treatment, 107.50(28.03-281.50) pg/mL after the 2nd week. There was no statistical difference between them(Z=-1.754, P>0.05). The serum IL-6 level was 64.05 (19.90-130.75) pg/mL after the 6th week and 24.80 (3.45-95.40) pg/mL after the 22nd week. Compared with pre-treatment, they were all lower than pre-treatment levels(Z=-2.942,-3.334,P 0.05,>0.05,>0.05). Serum IL-8 level was 200.85(95.43-364.00)pg/ml after the 2nd week, 194.50(50.75-433.00)pg/ml after the 6th week, 161.50 (38.98-308.00)pg/ml after the 22nd week. Compared with pre-treatment [96.20(59.75-371.75) pg/ml], there were no statistical difference between after and pre-treatment(Z=-0.86,-0.131,-0.186,P>0.05,>0.05,>0.05). There was no statistical difference between after the 2nd week and pre-treatment in the IL-10 level(χ2=2.33, P>0.05). The IL-10 level after 6th week and after 22nd week were all lower than pre-treatment levels(χ2=4.08, 4.08, P 0.05,>0.05,>0.05)in IL-1β level. \u0000 \u0000 \u0000Conclusion \u0000After treatment with tocilizumab, the levels of serum IL-6 and IL-2R are helpful to assess the activity of SoJIA and the efficacy of therapy. \u0000 \u0000 \u0000Key words: \u0000Cytokine; Tocilizumab; Systemic-onset juvenile idiopathic arthritis","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"33 1","pages":"85-90"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80783381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in diagnosis and treatment of DNA methylation and pulmonary fibrosis","authors":"Z. Xiang","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.01.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.01.011","url":null,"abstract":"Pulmonary fibrosis is the late stage of many lung diseases. It′s a pathological process with excessive deposition of extracellular matrix, tissue scar formation and lung tissue remodeling. Recently, DNA methylation has been demonstrated to involve in organ fibrosis, includingpulmonary, liver, renal fibrosis and so on. DNA methylation plays an important role in clinical diagnosis and treatment. This article reviews the role of DNA methylation in the development of pulmonary fibrosis and discusses thepathogenic mechanism of pulmonary fibrosis and new anti-fibrotic strategy. \u0000 \u0000 \u0000Key words: \u0000DNA methylation; Pulmonary fibrosis; Epigenetics","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"6 1","pages":"91-95"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78496038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of CYP2C9 and VKORC1 genetic polymorphisms in Chinese Han population on maintenance dosage of warfarin","authors":"Pengfei Liu, Z. Zhuo, Mingjie Su, Yan Long, Chang Liu","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.008","url":null,"abstract":"Objective \u0000To analyze CYP2C9 and VKORC1 gene polymorphisms in Chinese Han population and their correlation with the maintenance dosage of warfarin. \u0000 \u0000 \u0000Methods \u0000From October 2017 to April 2018, 458 Chinese Han patients (213 males and 245 females, aged from 26 to 94 years old) who underwent coagulation analysis in Peking University People′s Hospital were included in this retrospective study. PCR-Fluorescent probe method was applied to detect CYP2C9*3 and VKORC1-1639A>G gene polymorphisms in 458 patients, and among them, 130 patients who took warfarin for anticoagulant therapy and reached the international standard ratio of prothrombin time (INR) within the range of 2.0-3.0 were recorded. The basic information, dosage of warfarin and INR were also recorded. The statistical analysis data were compared with the reference table of recommended dosage of warfarin for different genotypes of patients recommended by FDA and the formula of predicted dosage of warfarin was simply verified by SPSS. \u0000 \u0000 \u0000Results \u0000Among the 458 patients who took anticoagulant therapy, the genotype frequencies of CYP2C9*1/*1(AA), CYP2C9*1/*3(AC) and CYP2C9*3/*3(CC) were 90.8%, 8.5%, and 0.7%; the genotype frequencies of VKORC1-1639GG and VKORC1-1639AG were 0.9% and 14.2%; the genotype frequencies of VKORC1-1639AA was 84.9%. After INR was reached, the results showed that the variant CYP2C9*1/*3 and CYP2C9*3/*3 required lower daily maintain dosage [(2.92±1.29) mg] than wild-type CYP2C9*1/*1 patients did [(3.91±1.63) mg], with statistically significant difference (P=0.018). And variant VKORC1-AA required lower daily maintain dosage [(3.68±1.64) mg] than variant VKORC1-AG patients did [(4.54±1.29) mg], with statistically significant difference (P=0.001). The application dosage of warfarin in patients with different VKORC1+CYP2C9 genotypes was consistent with the recommended dosage of the FDA reference table. The prediction accuracy of miao 2007 formula was lower than that of IWPC formula, and 94.1% of patients′ dosages of warfarin were underestimated. \u0000 \u0000 \u0000Conclusion \u0000Patients with CYP2C9*3 or VKORC1-AA genotype required lower warfarin dosage. The CYP2C9 and VKORC1 gene polymorphisms had a certain correlation with maintenance dosage of warfarin. \u0000 \u0000 \u0000Key words: \u0000Warfarin; Polymorphism, genetic; Cytochrome P-450 CYP2C9; Vitamin K epoxide reductases; Dose-respanse relationship,drug","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"55 1","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81278752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a predictive model for outcomes in patients with severe acute pancreatitis by nucleated red blood cells combined with Ranson score and APACHE II score","authors":"Jing Wang, X. Jin, G. Lu, Yuan Yuan","doi":"10.3760/CMA.J.ISSN.1009-8158.2020.01.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-8158.2020.01.007","url":null,"abstract":"Objective \u0000To explore a predictive model for outcomes of severe acute pancreatitis (SAP) patients. \u0000 \u0000 \u0000Methods \u0000A retrospective study was conducted of 102 SAP patients from January 1,2016 to April 30,2018 from Taizhou Hospital in this study. The participants were divided into survival group and death group according to the outcome of 90 days after admission,88 cases were in survival group including 57 males and 31 females, aged 55.5 (40.3-69.8) years; 14 cases were in death group including 5 males and 9 females, aged 63.0 (50.8-80.8) years. Clinical data and laboratory indicators were compared between the two groups. Statistical analyses were performed to compare categorical variables. Chi-square automatic interaction detector (CHAID) was used to construct the prediction model of SAP patients′ outcomes. The study cohort consisted of SAP patients from August 1st 2018 to July 1st 2019 were collected to validate the prediction model. \u0000 \u0000 \u0000Results \u0000(1)Statistical analyses were performed by chi square test and Mann-Whitney U test. There were statistically significant differences in the proportion of cardiovascular and cerebrovascular diseases, mechanical ventilation and septic shock, and also the Charlson complication index (CCI), the Ranson score and APACHEⅡscore(χ2=5.554, P=0.018; χ2=5.585,P=0.018;P=0.008;Z=-3.007,P=0.003;Z=-2.982, P=0.003; Z=-3.257, P=0.001), death group were higher than survival group. (2) The MPV, CRP, MCHC, pH, pCO2 and positive rates of NRBC were statistically different between survival group and death group(Z=-2.466,P=0.014;Z=-2.689,P=0.007;Z=-2.238,P=0.025;Z=-1.977,P=0.048;Z=-2.239, P=0.025;P=0.000).The NRBC-positive rate in the death group was higher than that in the survival group, while the other indexes were lower in the survival group.(3)The decision tree CHAID method obtains the prediction scheme: when it meets the Ranson score≤3, the SAP patients were judged to be alive; when it meets the Ranson score>3 and the NRBC in peripheral blood was negative, the SAP patients were judged to be alive; when it meets the Ranson score>3, the NRBC was positive and the APACHEⅡscore≤21, the SAP patients were judged to be alive; when it meets the Ranson score>3, NRBC was positive and APACHE Ⅱ>21, the SAP patients were adverse prognosis. (4) 50 SAP patients were collected in the validation group, with 43 actual survivors and 7 deaths. The accuracy rate of predicting the outcomes of SAP patients in validation group with the scheme was 94.0% (47/50). \u0000 \u0000 \u0000Conclusion \u0000The NRBC combined with Ranson score system and APACHE II score system can predict the outcomes of SAP patients. \u0000 \u0000 \u0000Key words: \u0000Acute disease; Pancreatitis; Erythroblasts; APACHE","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"1 1","pages":"63-70"},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87516035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of complement system in the diagnosis of systemic lupus erythematosus","authors":"Zhou Haizhou, Guan Xiu-ru","doi":"10.3760/CMA.J.ISSN.1009-9158.2019.12.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2019.12.006","url":null,"abstract":"The complement system plays a major role in systemic lupus erythematosus (SLE). At present, complement component 3(C3) and component 4(C4) are the diagnostic markers in the internationally recognized standard of SLE classification. Complement C3 and C4 can play a role in the diagnosis of SLE patients, but they are not very specific diagnostic markers. Therefore, it is important to find more better biomarkers of SLE. In this review, the latest findings in complement-focused research in SLE were explored. Complement level, cell-bound complement activation products and membrane-bound complement regulatory proteins are associated with the pathogenesis of SLE. They may become SLE biomarkers and aid the diagnosis and monitoring of patients with this disease. \u0000 \u0000 \u0000Key words: \u0000Lupus erythematosus, systemic; Complement system proteins; Complement activation; Biomarkers","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"315 1","pages":"1002-1006"},"PeriodicalIF":0.0,"publicationDate":"2019-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78283386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}