Neuroprotocols最新文献_第8页

Grafting Immortalized Hippocampal Cells into the Brain of the Adult and the Newborn Rat 成年和新生大鼠脑内移植永生海马细胞

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/ncmn.1993.1061

Cunningham Miles G., Nikkhah Guido, McKay Ronald D.G.

引用次数: 13

Isolation and Characterization of Glial Cell Lines from Xenopus Neuroepithelium and Retinal Pigment Epithelium 爪蟾神经上皮和视网膜色素上皮胶质细胞系的分离与鉴定

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/NCMN.1993.1060

D. Sakaguchi, E. Henderson

{"title":"Isolation and Characterization of Glial Cell Lines from Xenopus Neuroepithelium and Retinal Pigment Epithelium","authors":"D. Sakaguchi, E. Henderson","doi":"10.1006/NCMN.1993.1060","DOIUrl":"https://doi.org/10.1006/NCMN.1993.1060","url":null,"abstract":"Abstract We have isolated several immortal cell lines from Xenopus neuroepithelium and retinal pigment epithelium. These cell lines were initially isolated from primary cultures by serial passaging of proliferating cells, followed by subcloning with limiting dilution techniques. Several morphologically distinct cell lines have been isolated using these procedures. On the basis of immunocytochemical characterization using specific antibodies, we have established that three of these cell lines, the XR1, XRpe1, and XRpe2 cell lines, are glial-like in nature. These cell lines were extensively labeled by antibodies against glial fibrillary acidic protein and vimentin, markers used to identify glial cells. Mono-layers of these cell lines served as useful substrates for axon outgrowth from developing retinal ganglion cells. In addition, analysis of cell-free substrates, prepared by treatment of cell line monolayers with Triton X- 100, revealed that the XR1, XRpe1, and XRpe2 cell lines produce an extracellular matrix (ECM) with potent neurite outgrowth-promoting activity. In contrast, other established retinal and nonretinal Xenopus cell lines were relatively ineffective and did not support axon outgrowth. We propose that neurite outgrowth-promoting activity produced by these cell lines is associated with their ECM and may be glial cell specific. In addition, to further characterize these cell lines, we have recently imaged live cells, using the atomic force microscope (AFM). The use of AFM on living, cultured cells provides a new, high-resolution method for examining dynamic cytoskeletal and morphological events.","PeriodicalId":100951,"journal":{"name":"Neuroprotocols","volume":"164 8 1","pages":"249-259"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84185666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Immortalized Neuronal and Neuroendocrine Cell Lines by Targeted Oncogenesis in Transgenic Mice Using the PNMT Promoter 利用PNMT启动子在转基因小鼠中靶向肿瘤发生使神经元和神经内分泌细胞系永生

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/ncmn.1993.1052

Hammang Joseph P., Baetge E.Edward, Messing Albee

引用次数: 1

Grafting Immortalized Hippocampal Cells into the Brain of the Adult and the Newborn Rat 将永生化海马细胞移植到成年和新生大鼠的大脑中

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/NCMN.1993.1061

M. Cunningham, G. Nikkhah, R. McKay

引用次数: 13

Avian Retroviruses: Tools for Generating Immortalized Neural Cells and Studying Neural Differentiation 禽逆转录病毒：产生永生神经细胞和研究神经分化的工具

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/ncmn.1993.1058

Saule Simon, Fauquet Mireille

引用次数: 0

Cumulative Subject Index for Volumes 2–3 第2-3卷的累积主题索引

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/ncmn.1993.1063

引用次数: 0

Immortalization of Neural Cells with the c-myc and N-myc Proto-oncogenes c-myc和N-myc原癌基因对神经细胞的永生作用

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/ncmn.1993.1055

Bernard Ora

{"title":"Immortalization of Neural Cells with the c-myc and N-myc Proto-oncogenes","authors":"Bernard Ora","doi":"10.1006/ncmn.1993.1055","DOIUrl":"https://doi.org/10.1006/ncmn.1993.1055","url":null,"abstract":"<div><p>The c-<em>myc</em> and the N-<em>myc</em> proto-oncogenes were employed to immortalize neural progenitor cells. Infection of neural precursors isolated from the mouse at the 10th day of embryonic development (E10) with <em>myc</em>-containing retroviruses resulted in immortalized cell lines representing bipotential E10 neuroepithelial cells. These cell lines have the capacity to differentiate into both glial and neuronal cells either spontaneously in the case of the Zen(<em>myc</em>) cell lines or after addition of fibroblast growth factor to the Dol(<em>myc</em>) cell lines. Infection of migrating neural crest cells with the <em>myc</em> retroviruses gave rise to three different types of immortalized cell lines: (i) cell lines resembling freshly isolated neural crest cells; (ii) cell lines that can differentiate into cells expressing Schwann cell markers when grown at high cell concentrations; and (iii) cell lines that have the ability to differentiate in culture to process-bearing cells which expressed neuronal markers or have the characteristics of Schwann cells. Olfactory epithelial cell lines were generated by infection with Zen retrovirus bearing the N-<em>myc</em> proto-oncogene. Some of the cell lines resemble basal cells and others grow as bipolar cells resembling neurons and expressing the neuronal marker neurofilaments.</p></div>","PeriodicalId":100951,"journal":{"name":"Neuroprotocols","volume":"3 3","pages":"Pages 200-213"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/ncmn.1993.1055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72112749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Immortalization of Spinal Cord Motor Neurons by Fusion of Primary Neurons with Neuroblastoma Cell Lines 原代神经元与神经母细胞瘤细胞系融合的脊髓运动神经元永生化研究

Neuroprotocols Pub Date : 1993-12-01 DOI: 10.1006/NCMN.1993.1059

E. Salazar-Grueso

引用次数: 0

Optimizing Biological Activity of Lipophilic Compounds in Cultured Cells by Improving Delivery: Theoretical and Practical Considerations 通过改善递送优化培养细胞中亲脂化合物的生物活性：理论和实践考虑

Neuroprotocols Pub Date : 1993-10-01 DOI: 10.1006/ncmn.1993.1050

Buxser Stephen

{"title":"Optimizing Biological Activity of Lipophilic Compounds in Cultured Cells by Improving Delivery: Theoretical and Practical Considerations","authors":"Buxser Stephen","doi":"10.1006/ncmn.1993.1050","DOIUrl":"https://doi.org/10.1006/ncmn.1993.1050","url":null,"abstract":"<div><p>The use of lipophilic compounds in the study of signal transduction and other cellular processes is often complicated by the need to deliver compounds that are minimally soluble in water to cells in culture. A typical approach is to dissolve the lipophilic compound at high concentration in organic solvent and then to dilute the solvent in aqueous medium. However, such an approach usually results in precipitation of the compound in the aqueous medium and may minimize delivery of the compound to cells. Three techniques to keep the lipophilic compound In a nonprecipitated and stable form that will be readily available to the cells are described. They involve the use of protein carriers, liposomes, and emulsions. Particular attention is given to the use of emulsions, since this technique combines the advantages of a high capacity for lipophilic compounds, ease of assembly, and minimum contact between the lipophilic compound and water. Although the techniques differ with respect to the mechanics of combining the compound and the carrier, they all consist of a two-phase system dependent on partitioning between the carrier and the cells. Due to the need to take partitioning into account, all of these techniques differ from homogeneous solution-phase delivery. Therefore, in addition to descriptions of the techniques, criteria for both experimental design and analysis of data generated using two-phase systems are presented. In combination, the use of methods appropriate for delivery of lipophilic compounds to cells and the application of relevant calculations and analytical procedures provide the means necessary for design and Interpretation of experiments using lipophilic compounds.</p></div>","PeriodicalId":100951,"journal":{"name":"Neuroprotocols","volume":"3 2","pages":"Pages 165-174"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/ncmn.1993.1050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72082854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Regulation of Glycosyl-phosphoinositides in Mammalian Cells 哺乳动物细胞中糖基磷酸肌苷的调控

Neuroprotocols Pub Date : 1993-10-01 DOI: 10.1006/NCMN.1993.1045

C. Pearl, A. Saltiel

引用次数: 0