Mutation Research/DNA Repair Reports最新文献_第3页

A new mutagen-sensitive mutant in Neurospora, mus-16 神经孢子菌us-16一个新的诱变敏感突变体

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90051-X

Hirokazu Inoue , Alice L. Schroeder

引用次数: 6

Comparison of induction and repair of adducts and of alkali-labile sites in human lymphocytes and granulocytes after exposure to ethylating agents 暴露于乙基化剂后人淋巴细胞和粒细胞加合物和碱不稳定位点的诱导和修复比较

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90053-3

Henk H. Schutte, Govert P. van der Schans, Paul H.M. Lohman

{"title":"Comparison of induction and repair of adducts and of alkali-labile sites in human lymphocytes and granulocytes after exposure to ethylating agents","authors":"Henk H. Schutte, Govert P. van der Schans, Paul H.M. Lohman","doi":"10.1016/0167-8817(88)90053-3","DOIUrl":"10.1016/0167-8817(88)90053-3","url":null,"abstract":"<div>A comparative study has been made of the induction and repair of adducts and alkali-labile sites in the DNA of human lymphocytes and granulocytes exposed to the ethylating agents N-ethyl-N-nitrosourea (ENU) and diethyl sulphate (DES).To evaluate these damages, the human blood cells were treated with highly 3H-labelled ENU and DES, and the resulting 3H-ethyl adducts were analysed via HPLC. Alkali-labile sites introduced in the DNA during treatment with non-radioactive ENU and DES were detected by alkaline elution with fluorometric quantitation of the DNA in the eluted fractions.All known adducts induced by ENU and DES could be detected by the HPLC methods applied. Furthermore, these adducts were separated from a number of unidentified compounds, because of the improved resolution on the columns used. Most of the adducts were rather persistent during a subsequent incubation period of up to 20 h after treatment, but some partly disappeared (7-ethyladenine and 3-ethyladenine).The induction of alkali-labile sites in lymphocytes and granulocytes was very similar, but the kinetics of the removal of these sites appeared to be quite different. In granulocytes there was hardly any repair, whereas in lymphocytes, particularly after ENU treatment, a substantial and relatively fast repair was observed. Induction of alkali-labile sites in human lymphocytes and granulocytes occurred also at 0°C; the data suggest that this kind of damage is not a result of enzymic repair processes.A comparison of the induction and the repair of alkali-labile sites in lymphocytes and granulocytes with those of the various ethyl adducts did not give a clue as to the identity of the adduct that could be responsible for the lability towards alkali.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90053-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14513494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Intragenic suppression in the uvrD gene of Escherichia coli I. Temperature-sensitive uvrD mutations 大肠杆菌uvrD基因的基因内抑制ⅰ。温度敏感的uvrD突变

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90054-5

Yoshihiro Yamamoto, Miyako Yabuki, Jun-ichi Furuyama

引用次数: 2

Low levels of DNA excision repair in undamaged bovine lymphocytes 未受损牛淋巴细胞DNA切除修复低水平

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90057-0

T.J. Schrader, I.G. Walker

{"title":"Low levels of DNA excision repair in undamaged bovine lymphocytes","authors":"T.J. Schrader, I.G. Walker","doi":"10.1016/0167-8817(88)90057-0","DOIUrl":"10.1016/0167-8817(88)90057-0","url":null,"abstract":"<div>Quiescent and concanavalin A-stimulated bovine lymphocytes were subjected to a buoyant density analysis used in excision repair studies. Despite neutral and alkaline rebands to remove replicative contamination, the CsCl gradient profiles of DNA isolated from unstimulated lymphocytes given a 6-h labelling period revealed a small amount of radioactivity in the normal-density region which is indicative of an excision repair process. It amounted to the incorporation of 8,000–20,000 molecules of thymidine per lymphocyte. In a 12-h labelling period the extent of repair incorporation was twice that measured in a 6-h period. The extent of this repair incorporation was not altered significantly during the initial 6 or 12 h of lectin stimulation when DNA-strand breaks normally present in the unstimulated cells are repaired. The same amount of repair activity was found whether the measurements were made on the same day that the lymphocytes were isolated or on the next day following on overnight incubation of the cells in culture medium. These observations indicate that lymphocytes display a spontaneous excision repair activity that proceeds continuously and at a constant rate.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90057-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14513496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

DNA repair synthesis in primary culture of bovine bile duct epithelial cells induced by chemical agents in relation to bile duct cancer 化学制剂诱导原代培养牛胆管上皮细胞DNA修复合成与胆管癌的关系

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90058-2

Ming De Lü, Kohji Miyazaki, Soichi Yoshitomi, Fumio Nakayama

{"title":"DNA repair synthesis in primary culture of bovine bile duct epithelial cells induced by chemical agents in relation to bile duct cancer","authors":"Ming De Lü, Kohji Miyazaki, Soichi Yoshitomi, Fumio Nakayama","doi":"10.1016/0167-8817(88)90058-2","DOIUrl":"10.1016/0167-8817(88)90058-2","url":null,"abstract":"<div>Unscheduled DNA synthesis (UDS) was measured autoradiographically in a primary culture of extrahepatic bile duct epithelial cells of Holstein cows following exposure to chemicals known to be capable of developing bile duct cancers in experimental animals, i.e., N-methyl-N′-nitro-N-nitrosoguanidine (MNNG, CAS No. 70-25-7), N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG, CAS No. 4255-77-6), 20-methylcholanthrene (MCA, CAS No. 56-49-5), N-nitrosodimethylamine (DMN, CAS No. 62-75-9) and aflatoxin B1 (AFB1, CAS No. 1162-65-8). MNNG and ENNG induced UDS without addition of S9 mixture. MCA elicited UDS only if S9 mixture was added. Regardless of the presence or absence of S9 mixture, DMN failed to induce UDS. DNA repair by AFB1 was enhanced by the presence of S9 mixture. Therefore, for MNNG, ENNG and high doses of AFB1 activation by the liver is not necessary to exert genotoxic effects and they seem to be capable of direct action on bile duct epithelial cells in the presence of a bilioenteric fistula or anastomosis.</div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90058-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14512359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of 3-aminobenzamide on the rejoining of DNA-strand breaks in mammalian cells exposed to methyl methanesulphonate; role of poly(ADP-ribose) polymerase 3-氨基苯甲酰胺对甲基磺酸暴露的哺乳动物细胞dna链断裂重新连接的影响聚(adp -核糖)聚合酶的作用

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90052-1

Gunnar Ahnström, Mats Ljungman

引用次数: 18

Cellular responses to DNA damage in the yeast Saccharomyces cerevisiae 酵母对DNA损伤的细胞反应

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90050-8

Helmut J. Burtscher, A. Jane Cooper, Linda B. Couto

引用次数: 3

Inactivation by nitrogen mustard of plasmids introduced into normal and Fanconi's anaemia cells 引入正常和范可尼贫血细胞的质粒氮芥灭活

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90056-9

S.W. Dean, H.R. Sykes, A.R. Lehmann

引用次数: 9

Photorepair of ultraviolet radiation-induced pyrimidine dimers in corneal DNA 紫外线辐射诱导的角膜DNA中嘧啶二聚体的光修复

Mutation Research/DNA Repair Reports Pub Date : 1988-07-01 DOI: 10.1016/0167-8817(88)90055-7

Ronald D. Ley, Lee A. Applegate, Steven F. Freeman

引用次数: 16

The DNA-repair enzyme uracil-DNA glycosylase in the human hematopoietic system 人造血系统中的dna修复酶尿嘧啶- dna糖基化酶

Mutation Research/DNA Repair Reports Pub Date : 1988-05-01 DOI: 10.1016/0167-8817(88)90031-4

Juhani A. Vilpo

引用次数: 13