A new mutagen-sensitive mutant in Neurospora, mus-16

Hirokazu Inoue , Alice L. Schroeder
{"title":"A new mutagen-sensitive mutant in Neurospora, mus-16","authors":"Hirokazu Inoue ,&nbsp;Alice L. Schroeder","doi":"10.1016/0167-8817(88)90051-X","DOIUrl":null,"url":null,"abstract":"<div><p>A new gene, <em>mus-16</em>, is determined by the nitrogen mustard-sensitive Neurospora mutant of Baker, Parish and Curtis (1984) which is defective in the removal of DNA-DNA and DNA-protein crosslinks. This gene is on the left arm of linkage group V between <em>caf-1</em> and <em>lys-1</em>. The <em>mus-16</em>(JMB) mutant is sensitive to the alkylating agents methyl methanesulfonate (MMS) [dose reduction factor (drf) 8–10 ×], <em>N</em>-methyl-<em>N</em>′-nitro-<em>N</em>-nitrosoguanidine (drf 5–6 ×), the amino acid histidine and the drug hydroxyurea. It is not sensitive to ultraviolet-light, γ-irradiation, or mitomycin C (MMC). It shows normal spontaneous mutation rates but increased induction of mutation by MMS. Homozygous crosses are barren, showing no signs of sporulation. Mitotic spontaneous chromosome instability is increased. The <em>mus-6</em> mutation is similar to several non-excision repair-defective mutants in Neurospora. Some of these may be defective in repair of alkylation damage. The MMC data supports earlier data that in fungi MMC is incapable of forming DNA-DNA crosslinks.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90051-X","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/016788178890051X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6

Abstract

A new gene, mus-16, is determined by the nitrogen mustard-sensitive Neurospora mutant of Baker, Parish and Curtis (1984) which is defective in the removal of DNA-DNA and DNA-protein crosslinks. This gene is on the left arm of linkage group V between caf-1 and lys-1. The mus-16(JMB) mutant is sensitive to the alkylating agents methyl methanesulfonate (MMS) [dose reduction factor (drf) 8–10 ×], N-methyl-N′-nitro-N-nitrosoguanidine (drf 5–6 ×), the amino acid histidine and the drug hydroxyurea. It is not sensitive to ultraviolet-light, γ-irradiation, or mitomycin C (MMC). It shows normal spontaneous mutation rates but increased induction of mutation by MMS. Homozygous crosses are barren, showing no signs of sporulation. Mitotic spontaneous chromosome instability is increased. The mus-6 mutation is similar to several non-excision repair-defective mutants in Neurospora. Some of these may be defective in repair of alkylation damage. The MMC data supports earlier data that in fungi MMC is incapable of forming DNA-DNA crosslinks.

神经孢子菌us-16一个新的诱变敏感突变体
一个新的基因,mus-16,是由Baker, Parish和Curtis(1984)的氮芥菜敏感神经孢子菌突变体确定的,该突变体在去除DNA-DNA和dna -蛋白质交联方面存在缺陷。该基因位于ca -1和lys-1之间的连锁组V的左臂上。突变体mu -16(JMB)对烷基化剂甲基磺酸甲酯(MMS)[剂量减少因子(drf) 8-10倍]、n -甲基-n′-硝基-n -亚硝基胍(drf) 5-6倍)、氨基酸组氨酸和药物羟基脲敏感。它对紫外线、γ辐射或丝裂霉素C (MMC)不敏感。它显示正常的自发突变率,但增加诱变的MMS。纯合子杂交是不育的,没有产孢的迹象。有丝分裂自发性染色体不稳定性增加。mus-6突变类似于神经孢子虫的几种非切除修复缺陷突变。其中一些在修复烷基化损伤时可能存在缺陷。MMC数据支持了真菌中MMC不能形成DNA-DNA交联的早期数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信