Molecular Brain Research最新文献

Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus 尼罗罗非鱼中枢神经系统神经元型一氧化氮合酶cDNA的部分克隆及nNOS mRNA的区域分布

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.018

Loredana Bordieri , Maria Carmela Bonaccorsi di Patti , Rossella Miele , Carla Cioni

{"title":"Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus","authors":"Loredana Bordieri , Maria Carmela Bonaccorsi di Patti , Rossella Miele , Carla Cioni","doi":"10.1016/j.molbrainres.2005.09.018","DOIUrl":"10.1016/j.molbrainres.2005.09.018","url":null,"abstract":"<div>A constitutive NOS complementary DNA (cDNA) was partially cloned by RT-PCR from the brain of a teleost, the Nile tilapia (Oreochromis niloticus), using degenerate primers against conserved regions of NOS. The predicted 206-long amino acid sequence showed a high degree of identity with other vertebrate neuronal NOS (nNOS) protein sequences. In addition, phylogenetic analysis revealed that Nile tilapia NOS clustered with other known nNOS.Using the coupled reaction of semi-quantitative RT-PCR and Southern blotting, the basal tissue expression pattern of the cloned nNOS gene was investigated in discrete areas of the central nervous system (CNS) and in the heart and skeletal muscle tissue. As revealed, expression of nNOS transcripts was detected in all the CNS regions examined, whereas nNOS gene was not expressed in the heart and skeletal muscle. The distribution pattern of nNOS gene expression showed the highest expression levels in the forebrain followed by the optic tectum, the brainstem and the spinal cord, whereas scarce expression was detected in the cerebellum.Cellular expression of nNOS mRNA was analyzed in the CNS by means of in situ hybridization. According to the RT-PCR results, most nNOS mRNA expressing neurons are localized in the telencephalon and diencephalon, whereas in the mesencephalic optic tectum, the brainstem and the spinal cord, nNOS mRNA expressing neurons are relatively more scattered. A very low hybridization signal was detected in the cerebellar cortex.These results suggest that NO is involved in numerous brain functions in teleosts.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 123-133"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25690371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Tolloid-like 1 is negatively regulated by stress and glucocorticoids 应激和糖皮质激素负向调节类脂质1

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.016

Goichiro Tamura, Dawne Olson, Joel Miron, Timothy G. Clark

{"title":"Tolloid-like 1 is negatively regulated by stress and glucocorticoids","authors":"Goichiro Tamura, Dawne Olson, Joel Miron, Timothy G. Clark","doi":"10.1016/j.molbrainres.2005.09.016","DOIUrl":"10.1016/j.molbrainres.2005.09.016","url":null,"abstract":"<div>Glucocorticoids affect a variety of tissues to enable the organism to adapt to the stress. Hippocampal neurons contain glucocorticoid receptors and respond to elevated glucocorticoid levels by down-regulating the HPA axis. Chronically, however, stress is deleterious to hippocampal neurons. Chronically elevated levels of glucocorticoids result in a decrease in the number of dendritic spines, reduced axonal growth and synaptogenesis, and decreased neurogenesis in the hippocampus. Tolloid-like 1 (Tll-1) is a metalloprotease that potentiates the activity of the bone morphogenetic proteins (BMPs). Neurogenesis in the hippocampus of both developing and adult mammals requires BMPs. In this study, we demonstrate that Tll-1 expression is increased in mice that have increased neurogenesis. The Tll-1 promoter contains glucocorticoid response elements which are capable of binding to purified glucocorticoid receptor. Glucocorticoids decrease Tll-1 expression in vitro. Finally, prenatal stress leads to a decrease in Tll-1 mRNA expression in the hippocampus of adult female mice that is not observed in adult male mice indicating that Tll-1 expression is differentially regulated in males and females. The results of this study indicate that Tll-1 is responsive to glucocorticoids and this mechanism might influence neurogenesis in the hippocampus.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 81-90"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25690370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Molecular regulation of glutamate and GABA transporter proteins by clobazam during epileptogenesis in Fe+++-induced epileptic rats 氯巴唑对铁致癫痫大鼠癫痫发生过程中谷氨酸和GABA转运蛋白的分子调控

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.010

Taku Doi , Yuto Ueda , Jun Tokumaru , L. James Willmore

{"title":"Molecular regulation of glutamate and GABA transporter proteins by clobazam during epileptogenesis in Fe+++-induced epileptic rats","authors":"Taku Doi , Yuto Ueda , Jun Tokumaru , L. James Willmore","doi":"10.1016/j.molbrainres.2005.09.010","DOIUrl":"10.1016/j.molbrainres.2005.09.010","url":null,"abstract":"<div>To assess the molecular effects of the antiepileptic drug clobazam (CLB, 1,5-benzodiazepine), a benzodiazepine effective in the management of epilepsy, we performed a series of experiments using rats with chronic, spontaneous recurrent seizures induced by amygdalar injection of FeCl3. Experimental animals were treated for 14 days with CLB. We then measured the expression of glutamate and GABA transporter proteins and evaluated the changes that occurred in these proteins using both experimental and control animals. CLB treatment was associated with an increase in the production of GLT-1 in the contra-lateral hippocampus of animals receiving amygdalar FeCl3 and CLB treatment. CLB treatment up-regulated the GABA transporter GAT3 in the contra-lateral hippocampus of animals with chronic, recurrent seizures. In contrast, CLB had no effect on the expression of EAAC1 and GAT1 in the hippocampus or the cortex in control animal groups. Chronic epileptogenesis may be associated with down-regulation of the production of glial excitatory amino acid transporters, GLAST and GLT-1, proteins that cause increase in the basal extracellular concentrations of glutamate. Elevated GABA transporter expression results in increased reverse transport of GABA to the extracellular space during periods of excitation. In addition to allosteric activation of GABAA receptors, this study suggests that CLB might exhibit its antiepileptic action by increasing GLT-1 expression and GAT3 in the hippocampus of rats with chronic seizures.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 91-96"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25690372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Cocaine induction of ERK proteins in dorsal striatum of Fischer rats 可卡因对Fischer大鼠背纹状体ERK蛋白的诱导

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.08.015

Shirzad Jenab, Eugene D. Festa, Arbi Nazarian, Hui Bing K. Wu, Wei Lun Sun, Ruhal Hazim, Scott J. Russo, Vanya Quinones-Jenab

引用次数: 57

Adenoviral interneuronal transportation after retrograde gene transfer in mice 小鼠逆行基因转移后的腺病毒神经间转运

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.021

Li-Kai Tsai , Ming-Shiun Tsai , Song-Kun Shyue , Wuh-Liang Hwu , Hung Li

{"title":"Adenoviral interneuronal transportation after retrograde gene transfer in mice","authors":"Li-Kai Tsai , Ming-Shiun Tsai , Song-Kun Shyue , Wuh-Liang Hwu , Hung Li","doi":"10.1016/j.molbrainres.2005.09.021","DOIUrl":"10.1016/j.molbrainres.2005.09.021","url":null,"abstract":"<div>Although retrograde gene transfer from infected muscles to neurons by viral vectors has been known for years, it is still unknown whether interneuronal gene transportation of viral vectors occurs after retrograde gene transfer. To determine this, we injected adenoviral vectors carrying eGFP gene with or without a neural tracer into the right gastrocnemius muscles of mice. After 7 days, some spinal motor neurons were detected with green fluorescence but without the signal of neural tracer. In addition, nerves with green fluorescence could be noted in the right lumbosacral paraspinal muscles of viral-injected mice. The green fluorescence in the right lumbosacral paraspinal muscles might have resulted from retrograde gene transportation from the viral-injected gastrocnemius muscles to the spinal neurons, followed by interneuronal transfer and anterograde expression of eGFP in the axons belonging to neurons innervating the paraspinal muscles. This phenomenon of interneuronal transportation raises the possibility that we could treat motoneuron diseases by injection of viral vectors containing therapeutic genes into a few muscles resulting in widespread beneficial effects.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 151-155"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25696054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Scutellariae radix extracts suppress ethanol-induced caspase-11 expression and cell death in N2a cells 黄芩提取物抑制乙醇诱导的N2a细胞caspase-11表达和细胞死亡

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.006

Kyounglan Kang , Yeo Kyoung Oh , Ryowon Choue , Shin Jung Kang

{"title":"Scutellariae radix extracts suppress ethanol-induced caspase-11 expression and cell death in N2a cells","authors":"Kyounglan Kang , Yeo Kyoung Oh , Ryowon Choue , Shin Jung Kang","doi":"10.1016/j.molbrainres.2005.09.006","DOIUrl":"10.1016/j.molbrainres.2005.09.006","url":null,"abstract":"<div>Scutellariae radix is a Chinese herbal medicine that has been used to treat disease conditions accompanying inflammation and oxidative stress. In the present study, we examined the effect of Scutellariae radix extracts during acute ethanol exposure in N2a neuroblastoma. The Scutellariae radix extracts effectively inhibited ethanol-induced apoptosis and caspase-3/-7 activation. Ethanol induced the expression of caspase-11 that has been known as a dual regulator of pathological apoptosis and inflammatory response. The ethanol-induced caspase-11 expression was suppressed by pretreatment of the Scutellariae radix extracts. Furthermore, the activation of caspase-3/-7 and apoptosis were significantly inhibited in caspase-11−/− mouse embryonic fibroblasts following ethanol treatment. These results suggest that caspase-11 has a regulatory role in ethanol-induced apoptosis, and the suppression of caspase-11 may be a mechanism by which Scutellariae radix exerts its cytoprotective effect.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 139-145"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25692977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Brain Research Young Investigator Awards 脑研究青年研究者奖

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/S0169-328X(05)00432-8

引用次数: 0

Two estrogen replacement therapies differentially regulate expression of estrogen receptors alpha and beta in the hippocampus and cortex of ovariectomized rat 两种雌激素替代疗法对去卵巢大鼠海马和皮质中雌激素受体α和β表达的调节存在差异

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.013

Min Jin , Fan Jin , Li Zhang , Zhong Chen , Hefeng Huang

{"title":"Two estrogen replacement therapies differentially regulate expression of estrogen receptors alpha and beta in the hippocampus and cortex of ovariectomized rat","authors":"Min Jin , Fan Jin , Li Zhang , Zhong Chen , Hefeng Huang","doi":"10.1016/j.molbrainres.2005.09.013","DOIUrl":"10.1016/j.molbrainres.2005.09.013","url":null,"abstract":"<div>As estrogens have been implicated in altered cognitive function associated with menopause, the purpose of the present study was to determine the regulatory effects of different estrogen preparations on the expression of estrogen receptor subtypes in the hippocampus and cortex of ovariectomized rats. The expression of estrogen receptor mRNA and protein was determined with RT-PCR and immunohistochemistry, respectively. Two estrogen reagents, Premarin and Progynova, were used in the present study. Premarin, a conjugated equine estrogen, down-regulated ERα expression in the hippocampus and cortex of ovariectomized rats and had no effect on levels of ERβ expression in the same two regions. However, Progynova (valerate estradiol) was shown to up-regulate ERβ expression in the hippocampus and cortex and had no effect on the levels of ERα expression. Our present data suggest that different estrogen reagents used in estrogen replacement therapy could have different regulatory effects on the expression of estrogen receptor subtypes, which might, at least in part, explain why clinically, different estrogen preparations have distinct estrogenic effects on target organs.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 107-114"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25690700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Free colour illustrations in the online version of articles 免费彩色插图在文章的在线版本

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/S0169-328X(05)00431-6

引用次数: 0

IGF-I protects cortical neurons against ceramide-induced apoptosis via activation of the PI-3K/Akt and ERK pathways; is this protection independent of CREB and Bcl-2? IGF-I通过激活PI-3K/Akt和ERK通路保护皮质神经元免受神经酰胺诱导的凋亡;这种保护是否独立于CREB和Bcl-2?

Molecular Brain Research Pub Date : 2005-12-14 DOI: 10.1016/j.molbrainres.2005.09.020

Sandrine Willaime-Morawek, Nicolas Arbez, Jean Mariani, Bernard Brugg

{"title":"IGF-I protects cortical neurons against ceramide-induced apoptosis via activation of the PI-3K/Akt and ERK pathways; is this protection independent of CREB and Bcl-2?","authors":"Sandrine Willaime-Morawek, Nicolas Arbez, Jean Mariani, Bernard Brugg","doi":"10.1016/j.molbrainres.2005.09.020","DOIUrl":"10.1016/j.molbrainres.2005.09.020","url":null,"abstract":"<div>Current understanding of IGF-I-mediated neuroprotection implies the activation of phosphatidylinositol-3-kinase (PI-3K), which leads to the activation of Akt/Protein Kinase B. In non-neuronal cells, Akt phosphorylates and activates the transcription factor CREB, implicated in the transcription of the anti-apoptotic bcl-2 gene. This paper further analyses the anti-apoptotic IGF-I action in neurons. We show that IGF-I protects cortical neurons against ceramide-induced apoptosis. Ceramide decreases Akt phosphorylation during apoptotic process whereas a simultaneous treatment with IGF-I increases Akt phosphorylation. Analysis of the signal transduction pathways revealed that IGF-I induces CREB phosphorylation via PI-3K and ERK, whereas simultaneous ceramide and IGF-I treatment decreases CREB phosphorylation. Although an overexpression of Bcl-2 protects cortical neurons against ceramide-induced apoptosis, our data indicate that the Bcl-2 protein level is not modulated during IGF-I, ceramide and/or LY294002 treatment. In consequence, we demonstrated that IGF protects neurons against ceramide-induced apoptosis and that IGF-I protection involves the PI-3K/Akt and ERK pathways; this protection may be independent of CREB and Bcl-2.</div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 97-106"},"PeriodicalIF":0.0,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25693028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40