Molecular Brain Research最新文献

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Immunocytochemical localization of a neuron-specific diacylglycerol kinase β and γ in the developing rat brain 发育中的大鼠脑中神经元特异性二酰基甘油激酶β和γ的免疫细胞化学定位
Molecular Brain Research Pub Date : 2005-10-03 DOI: 10.1016/j.molbrainres.2005.06.007
Naoko Adachi, Miho Oyasu, Taizo Taniguchi, Yasuto Yamaguchi, Rika Takenaka, Yasuhito Shirai, Naoaki Saito
{"title":"Immunocytochemical localization of a neuron-specific diacylglycerol kinase β and γ in the developing rat brain","authors":"Naoko Adachi,&nbsp;Miho Oyasu,&nbsp;Taizo Taniguchi,&nbsp;Yasuto Yamaguchi,&nbsp;Rika Takenaka,&nbsp;Yasuhito Shirai,&nbsp;Naoaki Saito","doi":"10.1016/j.molbrainres.2005.06.007","DOIUrl":"10.1016/j.molbrainres.2005.06.007","url":null,"abstract":"<div><p><span><span>Diacylglycerol kinase (DGK) phosphorylates </span>diacylglycerol<span><span><span> (DG) to produce phosphatidic acid (PA) and is, therefore, a potential terminator of DG signaling. DG and PA are important intracellular second messengers. DG directly binds </span>protein kinase C (PKC) then activates this </span>multifunctional enzyme. Ca</span></span><sup>2+</sup><span><span>-dependent and brain-specific DGKs, α, β, and γ, are suggested to play pivotal roles in the central nervous system. To elucidate the DGK function in neuronal development, we studied the developmental changes of DGKα, β, and γ in the postnatal rat brain. By immunoblot analysis, DGKα and γ subtypes were present at birth and then gradually increased, while DGKβ was not present at birth or postnatal day 3, then increased rapidly from day 14 to reach maximum at day 28. Immunohistochemically, DGKβ and γ were distributed in different brain regions. In most brain regions, DGKγ showed sustained expression throughout the postnatal developmental periods. Interestingly, a temporal expression of DGKγ was observed in the medial geniculate nucleus<span> during day 3 to 14, and a delay of DGKγ expression was seen in Purkinje cells, which was coincident with dendritic growth of Purkinje cells. In the hippocampal </span></span>pyramidal cell<span><span>, both DGKβ and γ were abundant but subcellular localization was different. DGKγ localized in the cytosol while DGKβ localized along the </span>membrane structure. These findings suggest that each DGK subtype has a spatio-temporally different function in the developmental neurons.</span></span></p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Pages 288-299"},"PeriodicalIF":0.0,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.06.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24899332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 36
Gender-specific association of insertion/deletion polymorphisms in the nogo gene and chronic schizophrenia nogo基因插入/缺失多态性与慢性精神分裂症的性别特异性关联
Molecular Brain Research Pub Date : 2005-10-03 DOI: 10.1016/j.molbrainres.2005.05.010
Ene-Choo Tan , Siow-Ann Chong , Hanhui Wang , Eileen Chew-Ping Lim , Yik-Ying Teo
{"title":"Gender-specific association of insertion/deletion polymorphisms in the nogo gene and chronic schizophrenia","authors":"Ene-Choo Tan ,&nbsp;Siow-Ann Chong ,&nbsp;Hanhui Wang ,&nbsp;Eileen Chew-Ping Lim ,&nbsp;Yik-Ying Teo","doi":"10.1016/j.molbrainres.2005.05.010","DOIUrl":"10.1016/j.molbrainres.2005.05.010","url":null,"abstract":"<div><p><span><span>Nogo is a myelin-associated protein associated with neurite outgrowth and regeneration. A previous study has reported an association between an insertion/deletion polymorphism in </span>schizophrenia<span>. We tested for the distribution of the polymorphism and haplotypes of this and another insertion/deletion polymorphism in our population. We have also developed an assay combining allele-specific polymerase chain reaction (AS-PCR) and restriction fragment length polymorphism (RFLP) to simultaneously type these two insertion/deletion polymorphisms. There was a statistically significant difference at the allelic level for both the CAA (</span></span><em>χ</em><sup>2</sup> = 4.378, <em>df</em> = 1, <em>P</em> value = 0.036) and TATC (<em>χ</em><sup>2</sup> = 5.807, <em>df</em> = 1, <em>P</em> = 0.016) polymorphisms in the female subgroup, but not in males. With our genotyping method, we also determined the molecular haplotype. Within the female gender, odds ratio is at 1.57 (95% CI 1.05–2.37) for CAACAA-TATC and 1.40 (95% CI 0.55–3.60) for CAA-TATC, the two at-risk haplotypes. Odds ratio is 0.63 (95% CI 0.42–0.93) for the protective wildtype haplotype CAA-TATCTATC. Further study of these two polymorphisms to investigate functional significance and confirm gender-specific association should be carried out.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Pages 212-216"},"PeriodicalIF":0.0,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25134298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 37
Regulation of cAMP-induced arylalkylamine N-acetyltransferase, Period1, and MKP-1 gene expression by mitogen-activated protein kinases in the rat pineal gland 丝裂原活化蛋白激酶对大鼠松果体中camp诱导的芳基烷基胺n-乙酰转移酶、Period1和MKP-1基因表达的调控
Molecular Brain Research Pub Date : 2005-10-03 DOI: 10.1016/j.molbrainres.2005.06.004
Mathieu Chansard, Eiko Iwahana, Jian Liang, Chiaki Fukuhara
{"title":"Regulation of cAMP-induced arylalkylamine N-acetyltransferase, Period1, and MKP-1 gene expression by mitogen-activated protein kinases in the rat pineal gland","authors":"Mathieu Chansard,&nbsp;Eiko Iwahana,&nbsp;Jian Liang,&nbsp;Chiaki Fukuhara","doi":"10.1016/j.molbrainres.2005.06.004","DOIUrl":"10.1016/j.molbrainres.2005.06.004","url":null,"abstract":"<div><p><span><span>In rodent pineal glands, sympathetic innervation, which leads to </span>norepinephrine release<span>, is a key process in the circadian regulation of physiology and certain gene expressions. It has been shown that gene expression of the rate-limiting enzyme in the melatonin synthesis arylalkylamine </span></span><em>N</em>-acetyltransferase (<em>Aa-Nat</em>), circadian clock gene <em>Period1</em>, and mitogen-activated protein kinase (MAPK) phosphtase-1 (<em>MKP-1</em><span>), is controlled mainly by a norepinephrine-beta-adrenergic receptor-cAMP signaling cascade in the rat pineal gland. To further dissect the signaling cascades that regulate those gene expressions, we examined whether MAPKs are involved in cAMP-induced gene expression. Western blot and immunohistochemical analyses showed that one of the three MAPKs, c-Jun N-terminal kinase (JNK), was expressed in the pineal, and was phosphorylated by cAMP analogue stimulation with a peak 20 min after start of the stimulation, in vitro. A specific JNK inhibitor SP600125 (Anthra[1,9-</span><em>cd</em>]pyrazol-6(2<em>H</em>)-one1,9-pyrazoloanthrone), but not its negative control (<em>N</em><sup>1</sup>-Methyl-1,9-pyrazoloanthrone), significantly reduced cAMP-stimulated <em>Aa-Nat</em>, <em>Period1</em>, and <em>MKP-1</em> mRNA levels. Although another MAPK, p38<sup>MAPK</sup>, has also been shown to be activated by cAMP stimulation, a p38<sup>MAPK</sup> inhibitor, SB203580 (4-(4-Fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1<em>H</em>-imidazole, HCl), showed no effect on cAMP-induced <em>Aa-Nat</em> and <em>Period1</em> mRNA levels; whereas SB203580, but not its negative analogue SB202474 (4-Ethyl-2(<em>p</em>-methoxyphenyl)-5-(4′-pyridyl)-I<em>H</em>-imidazole, DiHCl), significantly reduced cAMP-induced <em>MKP-1</em> mRNA levels. Taken together, our data suggest that cAMP-induced <em>Aa-Nat</em> and <em>Period1</em> are likely to be mediated by activation of JNK, whereas <em>MKP-1</em> may be mediated by both p38<sup>MAPK</sup> and JNK activations.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Pages 333-340"},"PeriodicalIF":0.0,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.06.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25194093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Adrenergic receptor characterization of CA1 hippocampal neurons using real time single cell RT-PCR CA1海马神经元肾上腺素能受体的实时单细胞RT-PCR表征
Molecular Brain Research Pub Date : 2005-10-03 DOI: 10.1016/j.molbrainres.2005.05.033
Kristin L. Hillman , Chris A. Knudson , Patrick A. Carr , Van A. Doze , James E. Porter
{"title":"Adrenergic receptor characterization of CA1 hippocampal neurons using real time single cell RT-PCR","authors":"Kristin L. Hillman ,&nbsp;Chris A. Knudson ,&nbsp;Patrick A. Carr ,&nbsp;Van A. Doze ,&nbsp;James E. Porter","doi":"10.1016/j.molbrainres.2005.05.033","DOIUrl":"10.1016/j.molbrainres.2005.05.033","url":null,"abstract":"<div><p><span><span>The CA1 region of the rat hippocampus exhibits both α and β adrenergic receptor (AR) responses, however, the specific AR subtypes involved and the neuronal expression patterns for these receptors are not well understood. We have employed single cell real time RT-PCR in conjunction with cell-specific immunohistochemical markers to determine the </span>AR expression patterns for hippocampal neurons located in CA1, a region often implicated in learning and memory processes. Cytoplasmic samples were taken from 55 individual cells located in stratum oriens, pyramidale, or radiatum and reverse transcribed. All successfully amplified pyramidal neuron samples (</span><em>n</em> = 17) expressed mRNA for the β<sub>2</sub>AR, with four cells additionally expressing mRNA for the β<sub>1</sub><span>AR subtype. Positive interneurons from stratum oriens (</span><em>n</em> = 10) and stratum radiatum (<em>n</em> = 8) expressed mRNA for the α<sub>1A</sub> and/or α<sub>1B</sub>AR (<em>n</em><span><span> = 9/18) only when coexpressing transcripts for somatostatin. Interneurons containing </span>neuropeptide Y<span> or cholecystokinin (</span></span><em>n</em> = 9/18) were not positive for any of the nine AR subtypes, suggesting that CA1 interneuron AR expression is limited to a subset of somatostatin-positive cells. These findings suggest that only a select number of AR subtypes are transcriptionally expressed in CA1 and that these receptors are selective to specific neuronal cell types.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Pages 267-276"},"PeriodicalIF":0.0,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25178949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Free colour illustrations in the online version of articles 免费彩色插图在文章的在线版本
Molecular Brain Research Pub Date : 2005-10-03 DOI: 10.1016/S0169-328X(05)00349-9
{"title":"Free colour illustrations in the online version of articles","authors":"","doi":"10.1016/S0169-328X(05)00349-9","DOIUrl":"https://doi.org/10.1016/S0169-328X(05)00349-9","url":null,"abstract":"","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 2","pages":"Page iv"},"PeriodicalIF":0.0,"publicationDate":"2005-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0169-328X(05)00349-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137090257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymorphism of the untranslated regions of the F3/contactin mRNA in the rat nervous system 大鼠神经系统F3/contactin mRNA非翻译区多态性
Molecular Brain Research Pub Date : 2005-09-13 DOI: 10.1016/j.molbrainres.2005.05.012
Claire Rome , Valérie Roullot, Franck Couillaud
{"title":"Polymorphism of the untranslated regions of the F3/contactin mRNA in the rat nervous system","authors":"Claire Rome ,&nbsp;Valérie Roullot,&nbsp;Franck Couillaud","doi":"10.1016/j.molbrainres.2005.05.012","DOIUrl":"10.1016/j.molbrainres.2005.05.012","url":null,"abstract":"<div><p><span><span><span>F3/contactin is a neural adhesion molecule implicated in various physiological processes. In </span>rat brain tissues, we cloned various mRNA with the same coding region but differing in 3′ and 5′UTR. The 3′UTR presents two </span>polyadenylation<span> signals. At the 5′ end, we identified two leader exons, multiple transcription initiation sites and splicing events, leading to at least 19 different 5′UTR. The </span></span><em>F3</em>/<em>contactin</em> rat gene differs from the mouse gene for two reasons: (1) it contains two additional untranslated exons that are alternatively spliced and (2) it lacks the homologue mouse untranslated exon 0.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 1","pages":"Pages 184-191"},"PeriodicalIF":0.0,"publicationDate":"2005-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25145965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Distribution kinetics of 18F-DOPA in weaver mutant mice 18F-DOPA在weaver突变小鼠体内的分布动力学
Molecular Brain Research Pub Date : 2005-09-13 DOI: 10.1016/j.molbrainres.2005.05.018
Sushil K. Sharma, Manuchair Ebadi
{"title":"Distribution kinetics of 18F-DOPA in weaver mutant mice","authors":"Sushil K. Sharma,&nbsp;Manuchair Ebadi","doi":"10.1016/j.molbrainres.2005.05.018","DOIUrl":"10.1016/j.molbrainres.2005.05.018","url":null,"abstract":"<div><p>Distribution kinetics of <sup>18</sup>F-fluoro-dihydroxy phenylalanine (<sup>18</sup>F-DOPA) were studied with high-resolution micro-positron emission tomography (microPET) imaging and conventional methods in control wild-type mice, heterozygous weaver mutant mice, and homozygous weaver mutant mice. <sup>18</sup>F-DOPA uptake was significantly increased in the CNS within 60 min in all the genotypes examined. Homozygous weaver mutant mice exhibited significantly reduced <sup>18</sup>F-DOPA uptake in the region of interest (striatum) as compared to heterozygous weaver mutant mice and control wild-type mice. <sup>18</sup>F-DOPA was de-localized in the kidneys of homozygous weaver mutant mice. The radioactivity was localized primarily in the liver and kidneys within 2 h and in the urinary bladder within 4 h. After 8 h, it could be detected neither by conventional nor by microPET imaging. Distribution kinetics of <sup>18</sup>F-DOPA with microPET imaging correlated and confirmed the conventional observations. These data are interpreted to suggest that microPET imaging may provide an efficient, noninvasive, cost-effective procedure to study distribution kinetics of PET radiopharmaceuticals in rare genetically altered animals. Furthermore, this unique and noninvasive approach may expedite quality control and drug development for human applications.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 1","pages":"Pages 23-30"},"PeriodicalIF":0.0,"publicationDate":"2005-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25156552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Cloning and characterization of rat importin 9: Implication for its neuronal function 大鼠输入蛋白9的克隆与表征及其对神经元功能的影响
Molecular Brain Research Pub Date : 2005-09-13 DOI: 10.1016/j.molbrainres.2005.05.021
Elod Kortvely, Peter Burkovics, Szilvia Varszegi, Karoly Gulya
{"title":"Cloning and characterization of rat importin 9: Implication for its neuronal function","authors":"Elod Kortvely,&nbsp;Peter Burkovics,&nbsp;Szilvia Varszegi,&nbsp;Karoly Gulya","doi":"10.1016/j.molbrainres.2005.05.021","DOIUrl":"10.1016/j.molbrainres.2005.05.021","url":null,"abstract":"<div><p>We describe the structure of the rat <span><em>importin</em><em> 9</em></span><span> gene, together with its transcripts and the encoded protein with its putative functional domains. The </span><em>importin 9</em><span> gene contains 24 exons in a genomic region spanning &gt;52,000 bp. It is transcribed into two mRNAs, generated by means of alternative polyadenylation<span> site usage arranged in tandem. Both transcripts possess the same noncanonical polyadenylation signal (AGUAAA) in rat, this hexamer being conserved in all vertebrates examined. Additionally, intron 8 is bordered by AT–AC dinucleotides. </span></span><em>Importin 9</em><span><span> is expressed throughout adult rat tissues, but the 114-kDa Importin 9 protein was detected only in the brain. The localization of the Importin 9 protein was examined by immunohistochemistry in both adult rat tissues and primary hippocampal cell cultures. The strongest labeling was detected in vivo in areas populated by neurons in high density and also in the </span>dendritic processes<span><span> emanating from these cells. This protein was clearly concentrated in the nuclei of these cells, although their cytoplasms too were heavily labeled. Strong cytoplasmic and very strong nuclear staining was found in a vast majority of the cells with neuronal morphology in vitro. Cultured cells with glial morphology generally exhibited a weaker cytoplasmic labeling. In these cells, the signal decorated the </span>nuclear envelope without nuclear staining and gradually dwindled toward the cell periphery. These results hint at the cell- or tissue-type specific functions of this type of importin protein.</span></span></p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 1","pages":"Pages 103-114"},"PeriodicalIF":0.0,"publicationDate":"2005-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25166943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Suppression of long-term facilitation by Rab3–effector protein interaction rab3效应蛋白相互作用对长期促进的抑制
Molecular Brain Research Pub Date : 2005-09-13 DOI: 10.1016/j.molbrainres.2005.05.004
Jin-Hee Han, Changhoon Lee, Yehwang Cheang, Bong-Kiun Kaang
{"title":"Suppression of long-term facilitation by Rab3–effector protein interaction","authors":"Jin-Hee Han,&nbsp;Changhoon Lee,&nbsp;Yehwang Cheang,&nbsp;Bong-Kiun Kaang","doi":"10.1016/j.molbrainres.2005.05.004","DOIUrl":"10.1016/j.molbrainres.2005.05.004","url":null,"abstract":"<div><p>Long-term facilitation (LTF) in <span><em>Aplysia</em></span><span> is achieved by the modulation of presynaptic release. However, the underlying mechanism that might be related with the regulation of synaptic vesicle<span> release remains unknown. Since Rab3, a neuronal GTP-binding protein, is known to be a key regulator of synaptic vesicle fusion, we investigated the involvement of Rab3 in LTF. To address this issue, we examined the effect of overexpression of wild type </span></span><em>Aplysia</em><span> Rab3 (apRab3) and its mutant forms on LTF. Overexpression of either apRab3 Q80L, a constitutively active apRab3 mutant, or wild type apRab3 completely inhibited LTF. This inhibitory role of apRab3 appears to be mediated by an interaction with an effector molecule(s), possibly Rim. Expression of apRab3 Q80L, V54E double mutant, which do not bind effector molecules such as Rim or Rabphilin, had no effect on LTF. Furthermore, expression of apRab3 Q80L, F18L, D19E triple mutant, which has reduced binding activity with Rim but normally binds with Rabphilin, enhanced evoked basal synaptic release, and the increase in synaptic strength occluded LTF. In conclusion, our data suggest that apRab3 may act as a negative clamp of LTF through the interaction with effector protein(s), possibly Rim.</span></p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 1","pages":"Pages 13-22"},"PeriodicalIF":0.0,"publicationDate":"2005-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40948118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Functional characterisation of missense variations in the Kir4.1 potassium channel (KCNJ10) associated with seizure susceptibility 与癫痫易感性相关的Kir4.1钾通道(KCNJ10)错义变异的功能特征
Molecular Brain Research Pub Date : 2005-09-13 DOI: 10.1016/j.molbrainres.2005.05.003
Lijun Shang , Christopher J. Lucchese , Shozeb Haider , Stephen J. Tucker
{"title":"Functional characterisation of missense variations in the Kir4.1 potassium channel (KCNJ10) associated with seizure susceptibility","authors":"Lijun Shang ,&nbsp;Christopher J. Lucchese ,&nbsp;Shozeb Haider ,&nbsp;Stephen J. Tucker","doi":"10.1016/j.molbrainres.2005.05.003","DOIUrl":"10.1016/j.molbrainres.2005.05.003","url":null,"abstract":"<div><p>Recent genetic linkage studies have identified an association between missense variations in the gene encoding the Kir4.1 potassium channel (KCNJ10) and seizure susceptibility phenotypes in both humans and mice. The results of this study demonstrate that these variations (T262S and R271C) do not produce any observable changes in channel function or in predicted channel structure. It is therefore unlikely that the seizure susceptibility phenotypes associated with these missense variations are caused by changes in the intrinsic functional properties of Kir4.1.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"139 1","pages":"Pages 178-183"},"PeriodicalIF":0.0,"publicationDate":"2005-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40948602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
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