Medicine in Omics最新文献

{"title":"The omics technologies and liquid biopsies: Advantages, limitations, applications","authors":"Daniele Magro,&nbsp;Marika Venezia,&nbsp;Carmela Rita Balistreri","doi":"10.1016/j.meomic.2024.100039","DOIUrl":"10.1016/j.meomic.2024.100039","url":null,"abstract":"<div><p>In the recent years, the development of so-called omics technologies has greatly contributed to the discovery of new biomarkers and targets, spanning different areas from diagnosis to therapy, and helping to accelerate the progress of precision and personalized medicine. In addition to classic omics, including genomics, transcriptomics, proteomics, and metabolomics, newer-generation omics technologies and related platforms, such as microbiomics and nutrigenomics, are emerging. At the same time, the use of liquid biopsies is becoming established as optimal biological samples, consisting in biological fluids (i.e. blood, saliva, and urine), that are easy to collect, and whose components (cells, nucleic acids, exosome) can be analysed using throughput techniques. In addition, it is becoming attractive, because it consents the extrapolation of big data via multi-omics technologies. Here, we report a brief description and discussion of such technologies, highlighting applications and possible limitations.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100039"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000087/pdfft?md5=f30f9d627c62fdbe645f3283504dd6d2&pid=1-s2.0-S2590124924000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141135404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological storm and multiple organ damage by COVID-19","authors":"A. Yaser Arafath ,&nbsp;S. Aifa Fathima ,&nbsp;Anushara Prabhakaran ,&nbsp;Saqib Hassan ,&nbsp;Ragothaman Prathiviraj ,&nbsp;George Seghal Kiran ,&nbsp;Joseph Selvin","doi":"10.1016/j.meomic.2024.100034","DOIUrl":"10.1016/j.meomic.2024.100034","url":null,"abstract":"<div><p>The official recording outbreak COVID-19 virus was in Dec 2019. When it affects humans, it affects almost all age groups, especially aged people. COVID-19 becomes a Global pandemic within a short period. The primary consequence of this infection is that it targets the individual's respiratory system and causes severe acute respiratory syndrome (SARS-CoV-2). Research efforts were made internationally to find a proper vaccine. Here, with the mechanism of action, this review provides the infection mechanism, Immunological changes, and associated organ damage.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000038/pdfft?md5=0c89c281ca2a2de2fa36c1ddf2040e2d&pid=1-s2.0-S2590124924000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing of multi-Epitope vaccine against spike glycoprotein of SARS-CoV-2 using immunoinformatics approach","authors":"Deepak Kumar Jha ,&nbsp;Niti Yashvardhini ,&nbsp;Amit Kumar ,&nbsp;Manjush Gaurav ,&nbsp;Kumar Sayrav","doi":"10.1016/j.meomic.2024.100036","DOIUrl":"10.1016/j.meomic.2024.100036","url":null,"abstract":"<div><h3>Background</h3><p>COVID-19 caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome) has created an alarming situation worldwide. The surface (S) glycoprotein of novel coronavirus, encoded by the genome of SARS-CoV-2, plays an role in attachment, fusion as well as entry into the host cell. The spike glycoprotein plays vital role in not only infection but pathogenesis and adaptive immunity, and, therefore, the S glycoprotein is considered as the main target for the development of effective and durable vaccine against SARS-CoV-2. Present study aims to compare the SARS-CoV-2 spike sequence obtained from first Wuhan virus with those of Asian SARS-CoV-2 isolates.</p></div><div><h3>Result</h3><p>A total of 1165 mutations from 657 sequences of Asia submitted in the month of November 2020 to February 2021 were detected. Further, secondary structure prediction followed by protein modeling analysis was performed which revealed, these mutations, considerably altered the stability of Spike protein. Additionally, Physiochemical properties, toxicity, allergenicity and stability of spike glycoprotein were estimated to demonstrate the specificity of the epitope candidates. Subsequently, we identified a total of 34B-cell and 10 T-cell immune epitopes. Among the predicted epitopes, 26 B-cell and 9T-cell epitopes showed non-allergenic, non-toxic and highly antigenic properties.</p></div><div><h3>Conclusion</h3><p>Taken together, our study showed spike glycoprotein of SARS-CoV-2 can be a potentially good candidate for the development of vaccine to curb SARS-CoV-2 infections.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000051/pdfft?md5=789563bd81cf8146de57a8c567f2aead&pid=1-s2.0-S2590124924000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systems medicine framework for repurposable drug combinations for COVID-19 comorbidities","authors":"S. Saranya,&nbsp;L. Thamanna,&nbsp;P. Chellapandi","doi":"10.1016/j.meomic.2024.100038","DOIUrl":"10.1016/j.meomic.2024.100038","url":null,"abstract":"<div><p>Currently, vaccines have shown efficacy against new SARS-CoV-2 variants. This study aimed to develop a systems medicine framework that can predict and validate drug combinations that can be repurposed for treating COVID-19 and its comorbidities, specifically type 2 diabetes and hypertension. This study found that gut microbes could potentially influence the action of drugs, innate immune response, intestinal dysfunction, and susceptibility to the virus in individuals with these comorbidities. It was also discovered that the spike protein of the virus interacts with 57 human genes, many of which are linked to food-borne bacteria. An analysis of disease enrichment showed that arthritis and hypertension were frequently observed as comorbidities in patients infected with SARS-CoV-2. Several drugs, including Fluvoxamine, Donepezil, and Ifenprodil, have been identified as potentially repurposable drugs for treating COVID-19 in individuals with hypertension. Moreover, nitazoxanide and tocilizumab (antivirals), bacitracin (antibacterial), and gliclazide (antidiabetics) were also identified as potential repurposable drugs. Tocilizumab and gliclazide are effective drug combinations for treating COVID-19 in individuals with type 2 diabetes. A combination of tocilizumab and lidocaine has also been suggested for treating COVID-19 in individuals infected with food-borne bacteria.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000075/pdfft?md5=c548377f45b6d4562ed5e05c6f4c1cc5&pid=1-s2.0-S2590124924000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141142783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological and histomorphometric evaluation of the therapeutic role of sulforaphane on the hypothalamic-pituitary-ovarian axis in polycystic ovary syndrome rats via its potential anti-inflammatory and anti-oxidant activities","authors":"Sunday Aderemi Adelakun ,&nbsp;Ogunlade Babatunde ,&nbsp;Chukwunenye Chidi Chinazo ,&nbsp;Olukayode Abimbola Arowosegbe ,&nbsp;Damilola Obanijesu Adisa","doi":"10.1016/j.meomic.2023.100029","DOIUrl":"10.1016/j.meomic.2023.100029","url":null,"abstract":"<div><p>Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism and insulin resistance. Sulforaphane (SFN) is a widely distributed isothiocyanate found in cruciferous vegetables. This study investigated the role of SFN on the hypothalamic-pituitary-ovarian axis in PCOS rats. Twenty-four female rats randomized into group A control received 2 ml of normal saline. Group B received 4 mg/kg body weight (bwt) I.P of estradiol valerate (EV) on the first day, groups C received 100 mg/kg bwt of SFN orally, and Group D received I.P 4 mg/kg bwt of EV follow by 100 mg/kg bwt of SFN orally. All administrations were done once daily for 30 days. Final body weight, estrous cycle, ovarian weight, blood glucose, serum sex hormones levels, serum lipid profile, ovarian antioxidants assay, ovarian inflammatory cytokines, and ovary and pituitary histology were assessed.</p><p>The PCOS rats exhibited the characteristic features of PCOS. Disturbed ovarian cyclicity, histopathological alterations, decreased the number of healthy follicles and corpora lutea and increased degenerated and cystic follicles were detected by light microscopic studies. A significant increase in final body weight, ovarian weight, blood glucose, sex steroids hormone levels, and antioxidant assays in PCOS rats. Sulforaphane reduces the elevated level of serum sex steroids hormone, lipid profile, ovarian diameter, and cysts and restores healthy follicles in PCOS rats. SFN reduces the upraised levels of ovarian oxidant and inflammatory cytokines and increases the suppressed antioxidant enzymes.</p><p>Sulforaphane thus ameliorates the disturbed hormonal levels, lipid profile, and antioxidant status in PCOS rats through its antioxidant and anti-inflammatory capacity.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259012492300010X/pdfft?md5=0287a765b122edb77d6f064071b38b83&pid=1-s2.0-S259012492300010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136092829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic insights into heart development and cardiovascular diseases","authors":"Sona Charles ,&nbsp;Jeyakumar Natarajan","doi":"10.1016/j.meomic.2023.100026","DOIUrl":"10.1016/j.meomic.2023.100026","url":null,"abstract":"<div><p>The heart is a complex organ that starts to function by the end of the third week of gestation. Cardiovascular diseases attribute to the leading causes of death globally. The explosion of technological advances and computational tools for the analysis of next generation sequencing data has opened new avenues for comprehending the underlying regulatory mechanisms of complex disorders. The transcriptomic and genomic aspects of genes, non-coding RNAs and transcription factors have largely assisted understanding cardiogenesis and cardiovascular disease. The widespread application of transcriptomic methods has assisted in the screening of huge number of differentially expressed non-coding RNAs and their projected role as therapeutic targets and biomarkers. Transcriptomic profiling, including techniques such as RNA sequencing (RNA-Seq) has enabled the comprehensive characterization of gene expression patterns at various stages of embryonic and postnatal heart development. In the context of cardiac diseases, transcriptomic analyses have provided unprecedented insights into the molecular alterations associated with conditions such as myocardial infarction, heart failure, arrhythmias, and congenital heart defects. This review presents an overview of recent advancements in transcriptomic research aimed at unraveling the intricate genetic and molecular networks governing heart development, as well as in cardiac diseases. In this review, we attempted to systematically review the transcriptomic experiments performed to identify significant genes, miRNAs and lncRNAs with biomarkers therapeutic potential, <em>meta</em>-analysis of multiple samples, and ceRNA networks in heart diseases.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259012492300007X/pdfft?md5=75604034cce55490ebdf16d0530d043d&pid=1-s2.0-S259012492300007X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of effect of gene mutations related to telomere maintenance in gastric cancer patients","authors":"Anju R. Nath,&nbsp;Jeyakumar Natarajan","doi":"10.1016/j.meomic.2023.100025","DOIUrl":"10.1016/j.meomic.2023.100025","url":null,"abstract":"<div><h3>Background</h3><p>Mutations in gastric cancer may remain harmless despite mutations that can turn oncogenic. Although omics technologies have supplied a clear view of mutations and driver genes in cancers, the pathology of gastric cancer still is unknown.</p></div><div><h3>Objective</h3><p>This study aims to understand mutations of genes that relate to telomere maintenance in gastric cancer.</p></div><div><h3>Methods</h3><p>We used transcriptomic and proteomic data from normal and gastric cancer patients to decide the relationship between genes and proteins. We ranked the gene-protein pairs with the Spearman correlation and subjected the differently ranked genes for functional analysis. We performed the co-expression analysis to select the genes from functional analysis. Then we assessed the selected genes for mutations and thereafter neoantigen analysis.</p></div><div><h3>Results</h3><p>We identified 20 genes through differential ranking based on Spearman correlation of gene-protein pairs. Among these, ERCC1, MRE11A, RAD50, TP53BP1, and YWHAE were linked to critical functions like DNA repair, apoptosis, and stress response. Gene prioritization focused on these five genes, and from them through gene co-expression, RAD50, TP53BP1, and YWHAE were selected. Mutational analysis revealed prevalent C &gt; T and G &gt; A mutations. These genes were associated with elderly patients, specific histologic grades, and types of gastric cancer. Importantly, these mutations generated neoantigens with potential for immunotherapeutic targeting.</p></div><div><h3>Conclusion</h3><p>Our extensive research shows the complex genetic makeup of gastric cancer and its potential effects on diagnosis and treatment. The identified genes and the mutations linked to them offer important information on the molecular processes behind gastric cancer progression. These results set the foundation for additional research and immunotherapeutic applications in the field of gastric cancer.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000068/pdfft?md5=9d115915f82ec87beae4dbf57cf84944&pid=1-s2.0-S2590124923000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135654955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1H NMR metabolomics techniques demonstrated the effectiveness of methoxy substituted 2-benzylidene-1-indanone, A1 and A2A Adenosine Receptor antagonists, in modulating glucose production in the liver of diabetic rats","authors":"Olakunle Sanni ,&nbsp;David D. N'Da ,&nbsp;Jeremie Z. Lindeque ,&nbsp;Gisella. Terre'Blanche","doi":"10.1016/j.meomic.2023.100028","DOIUrl":"10.1016/j.meomic.2023.100028","url":null,"abstract":"<div><p>The physiological function of the liver in the overall disposal of postprandial glucose is important in the management of diabetes. Our recent research showed that benzylidene indanone derivative 2-(3,4-dihydroxybenzylidene)-4-methoxy-2,3-dihydro-1H-inden-1-one (2-BI) showed antihyperglycemic activity, but its role in glucose homeostasis in the liver is unknown. ¹H NMR metabolomics approach was used to unravel the effectiveness of 2-BI, on hepatic glucose production in fructose-streptozotocin (STZ) diabetics. Diabetes was induced in Sprague–Dawley rats using fructose-streptozotocin. Metabolites were extracted from the liver tissue and analyzed by ¹H NMR spectroscopy. Pathway analysis was performed on the identified metabolites. The unsupervised principal components analysis score plot showed clear differentiation. The control group (NC) was clearly separated from the diabetic group (DBC) and was clustered near the treated diabetic groups (DBI and DGT). There is a significant (p &lt; 0.01) increased level of 12 metabolites in diabetes rats that are crucial in liver glucose homeostasis. Treatment with 2-BI was able to lower the level of these metabolites. Detailed pathway analysis of the spiked metabolite levels shows an effect on the glycolysis/gluconeogenesis, butanoate, amino acid, nitrogen, and nucleotide (pyrimidine) metabolism. 2-BI reduced hyperglycemia in diabetic rats via the attenuation of hepatic glucose production and advancement of liver insulin sensitivity.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000093/pdfft?md5=bb8dbc5470a647fbf9b8f716cb174fba&pid=1-s2.0-S2590124923000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136059260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inferring molecular mechanisms of host-microbe-drug interactions in the human gastrointestinal tract","authors":"B. Roja,&nbsp;S. Saranya,&nbsp;L. Thamanna,&nbsp;P. Chellapandi","doi":"10.1016/j.meomic.2023.100027","DOIUrl":"10.1016/j.meomic.2023.100027","url":null,"abstract":"<div><p>This study aimed to elucidate the metabolic interplay between food-borne bacteria, gut methanogens, and probiotic bacteria using the host-microbe-drug interactome. This study also aimed to identify suitable drug combinations that could effectively combat food-borne infections without adversely affecting the normal gut microbes. In this study, the system medicine framework comprised 2654 edges and 1609 nodes, with 1370 interacting human genes. Through network modeling analysis, we identified interactions among 39 human target genes for food-borne bacteria, 11 targets for gut methanogens, and nine targets for probiotic bacteria. Gut methanogens target common human genes for their pathophysiological functions. Linoleic acid has emerged as a crosstalk metabolite that determines the abundance of foodborne bacteria. Gut microbes commonly share butyric acid, CO₃, and formaldehyde as metabolic precursors. Most antibiotics interact with human genes that target the gut methanogens and probiotic bacteria. Our study identified fusidic acid, nabiximol, oxacillin, ampicillin, phenoxymethylpenicillin, and cefdinir as repurposable antibiotics against foodborne bacterial infections. Chloroquine, an antimalarial drug, has been suggested as a potential repurposable drug for foodborne infections, owing to its indirect association with many foodborne bacteria via host-mediated interactions. Thus, our system medicine framework could potentially aid in suggesting repurposable antibiotics against food-borne bacterial infections, without affecting beneficial microbes in the human gastrointestinal tract.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000081/pdfft?md5=94bb8f9882f44d3d6830b27127295383&pid=1-s2.0-S2590124923000081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136056282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the genotypic variants and proteomic mutations in Turkish SARS-CoV-2 driving evolution and virulence","authors":"Ragothaman Prathiviraj ,&nbsp;K. Prinsha ,&nbsp;Saqib Hassan ,&nbsp;S. Hari Krishna Kumar ,&nbsp;George Seghal Kiran ,&nbsp;Joseph Selvin","doi":"10.1016/j.meomic.2023.100024","DOIUrl":"https://doi.org/10.1016/j.meomic.2023.100024","url":null,"abstract":"<div><p>Turkey is a transcontinental nation with much of its territory in western Asia and southeast Europe, and it is the 6th most affected country by SARS-CoV-2 globally, so far, many mutations have occurred within this short period of time. The present study mainly focused on identifying genotypic variants and proteomic mutations, along with the folding rate and virulence action of Turkey SARS-CoV-2 isolates. The phylogenomic study reveals that the first origination of SARS-CoV-2 isolates (2020) in Turkey has more chance of an occurrence of mutation rate during evolution/new origination. 53 genotypic- and 45 proteomic variants among 83 isolates were found. The genotypic and proteomic variants from 2022 isolates have six times higher mutation rates than 2020 isolates. The highest number of mutations occurred in the 2022 isolate (ERAGEM-OM-1104/2022), with 60 mutations in the G53 variant, followed by 44 mutations in the G49 variant (ERAGEM-DEL-1103/2021) and 42 mutations in the G50 variant (Ank_DLT/2021). Also, we identified 38 novel mutations among 45 proteomic variants. The predicted virulence mechanism of some ORFs such as Nsp1, Nsp3, Nsp4, Nsp5, Nsp8, 2′O-ribose, Spike, Orf3a, Envelope, Orf7b, Orf8, and Nucleocapsid shows high virulence and transmission rate. Hence, our findings would help the researchers to develop new anti-virals against new variants from Turkey’s SARS-CoV-2 isolates.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"9 ","pages":"Article 100024"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50188321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}