Medicine in Omics最新文献

{"title":"Designing of multi-Epitope vaccine against spike glycoprotein of SARS-CoV-2 using immunoinformatics approach","authors":"Deepak Kumar Jha ,&nbsp;Niti Yashvardhini ,&nbsp;Amit Kumar ,&nbsp;Manjush Gaurav ,&nbsp;Kumar Sayrav","doi":"10.1016/j.meomic.2024.100036","DOIUrl":"10.1016/j.meomic.2024.100036","url":null,"abstract":"<div><h3>Background</h3><p>COVID-19 caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome) has created an alarming situation worldwide. The surface (S) glycoprotein of novel coronavirus, encoded by the genome of SARS-CoV-2, plays an role in attachment, fusion as well as entry into the host cell. The spike glycoprotein plays vital role in not only infection but pathogenesis and adaptive immunity, and, therefore, the S glycoprotein is considered as the main target for the development of effective and durable vaccine against SARS-CoV-2. Present study aims to compare the SARS-CoV-2 spike sequence obtained from first Wuhan virus with those of Asian SARS-CoV-2 isolates.</p></div><div><h3>Result</h3><p>A total of 1165 mutations from 657 sequences of Asia submitted in the month of November 2020 to February 2021 were detected. Further, secondary structure prediction followed by protein modeling analysis was performed which revealed, these mutations, considerably altered the stability of Spike protein. Additionally, Physiochemical properties, toxicity, allergenicity and stability of spike glycoprotein were estimated to demonstrate the specificity of the epitope candidates. Subsequently, we identified a total of 34B-cell and 10 T-cell immune epitopes. Among the predicted epitopes, 26 B-cell and 9T-cell epitopes showed non-allergenic, non-toxic and highly antigenic properties.</p></div><div><h3>Conclusion</h3><p>Taken together, our study showed spike glycoprotein of SARS-CoV-2 can be a potentially good candidate for the development of vaccine to curb SARS-CoV-2 infections.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000051/pdfft?md5=789563bd81cf8146de57a8c567f2aead&pid=1-s2.0-S2590124924000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of nickel nanoparticles of Lactobacillus plantarum on VDR/LncRNA EIF3J-DT in Colorectal cancer","authors":"","doi":"10.1016/j.meomic.2024.100032","DOIUrl":"10.1016/j.meomic.2024.100032","url":null,"abstract":"<div><p>Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Vitamin D receptor (VDR) gene mutations and Vitamin D deficiency contribute to CRC development and progression. Certain long non-coding RNAs (lncRNAs) directly inhibit VDR gene transcription, leading to VDR mutation. Thus, targeting oncogenic lncRNAs and VDR expression is a promising strategy for effective cancer treatment. Here, we green-synthesized <em>Lactobacillus plantarum</em> loaded nickel oxide nanoparticles (LpNiONPs) to assess their anticancer potential in CRC by targeting long non-coding RNA EIF3J- divergent transcript (lncRNA EIF3J-DT) and VDR. The potent bioactive component present in <em>L. plantarum</em> was identified via gas chromatography-mass spectrometry (GC–MS) analysis, and its interaction with VDR, as well as the functional interaction with lncRNA EIF3J-DT, were evaluated using the PyRx program and RPISeq-software, respectively. The LpNiONPs were characterized using UV–Vis spectroscopy, Zeta Potential, dynamic light scattering (DLS), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) techniques. The anticancer potential of LpNiONPs against HT-29 cells was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, scratch assay, 4′,6-diamidino-2-phenylindole (DAPI)/ acridine orange-ethidium bromide (AO-EtBr) staining experiments, and reverse transcriptase-PCR to evaluate the expression of lncRNA EIF3J-DT/VDR and apoptotic-related genes. The potent bioactive compound Pyrrolo (1,2-a) pyrazine-1,4-dione in <em>L. plantarum</em> strongly interacts with VDR, highlighting its drug design potential. The formation of LpNiONPs was confirmed via UV–Vis spectroscopy with an absorption peak at 394 nm. LpNiONPs were positively charged, monodispersed, and stable square-shaped nanoparticles. LpNiONPs show dose-dependent cytotoxicity and induced apoptosis, confirmed by staining images in HT-29 cells. Moreover, LpNiONPs downregulated lncRNA EIF3J-DT, CYP24-A1 and BCL2 genes while upregulating VDR, cas-3, cas-9 and BAX in HT-29 cells. These findings suggest that LpNiONPs exhibit anticancer activity by promoting VDR-associated apoptosis by inhibiting lncRNA EIF3J-DT in CRC cells.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"12 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000014/pdfft?md5=de8b44eec910b4b98c932f9d9ae122af&pid=1-s2.0-S2590124924000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139874261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological storm and multiple organ damage by COVID-19","authors":"A. Yaser Arafath ,&nbsp;S. Aifa Fathima ,&nbsp;Anushara Prabhakaran ,&nbsp;Saqib Hassan ,&nbsp;Ragothaman Prathiviraj ,&nbsp;George Seghal Kiran ,&nbsp;Joseph Selvin","doi":"10.1016/j.meomic.2024.100034","DOIUrl":"10.1016/j.meomic.2024.100034","url":null,"abstract":"<div><p>The official recording outbreak COVID-19 virus was in Dec 2019. When it affects humans, it affects almost all age groups, especially aged people. COVID-19 becomes a Global pandemic within a short period. The primary consequence of this infection is that it targets the individual's respiratory system and causes severe acute respiratory syndrome (SARS-CoV-2). Research efforts were made internationally to find a proper vaccine. Here, with the mechanism of action, this review provides the infection mechanism, Immunological changes, and associated organ damage.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"11 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000038/pdfft?md5=0c89c281ca2a2de2fa36c1ddf2040e2d&pid=1-s2.0-S2590124924000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The integration of omics: A promising approach to personalized tuberculosis treatment","authors":"","doi":"10.1016/j.meomic.2024.100033","DOIUrl":"10.1016/j.meomic.2024.100033","url":null,"abstract":"<div><p>Tuberculosis (TB) continues to be a global health problem due to its high morbidity and death rates. Standardized regimens have been used in traditional TB treatment methods, frequently leading to less-than-ideal results and the establishment of drug-resistant strains. The development of personalized medicine provides a potentially effective remedy to individual patients' by adjusting therapeutic approaches to particular genotypic and phenotypic traits. Detecting TB strains, drug resistance indicators, and host genetic variants that affect treatment results is made possible by genomic and molecular diagnostic approaches. These developments offer helpful information for predicting therapy outcomes and choosing the best treatment plan for each individual.</p><p>Integrating phenotypic data, such as clinical characteristics, immunological state, and comorbidities, improves diagnostic and treatment decision-making accuracy. The use of targeted drug therapies, such as innovative anti-TB medicines and repurposed medications, which have the potential to overcome drug resistance and boost treatment effectiveness, can be guided by personalized therapy. Personalized interventions based on genetic and phenotypic factors can improve patient outcomes by identifying those at risk of treatment failure or disease progression. This article discusses the importance of personalized therapy for TB patients. It specifically highlights the benefits of using “omics” data to enhance therapeutic results and decrease the risk of drug resistance.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"12 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124924000026/pdfft?md5=f9154df8ec391a91cdbae2ebca0ed387&pid=1-s2.0-S2590124924000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139540750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated network pharmacology and molecular modelling study of phytoconstituents targeting Alzheimer's disease","authors":"Saumya Khanna,&nbsp;Divakar Selvaraj,&nbsp;Mehak Tyagi,&nbsp;Devadharshini,&nbsp;Saravanan Jayaram","doi":"10.1016/j.meomic.2023.100031","DOIUrl":"10.1016/j.meomic.2023.100031","url":null,"abstract":"<div><p>The present study involves the use of combined network pharmacology and molecular modelling approach for identifying important phytoconstituents that could modulate the functions of multiple therapeutic targets in Alzheimer’s disease. A list of botanicals reported in the literature for their efficacy in Alzheimer’s disease, the phytochemicals present in the botanicals were identified with the help of network pharmacology approach. The pharmacokinetic properties like blood brain barrier penetration and Lipinski’s rule of five for the selected phytoconstituents were analyzed. The major targets involved in the pathogenesis of Alzheimer’s disease were collected from the DisGeNET database. The selected proteins were subjected to topological analysis using Cytoscape software to identify the important targets in the network. The top 7 phytoconstituents and 5 proteins were subjected to molecular docking, MM-GBSA and molecular dynamics studies. A total of 15 plants and 1443 phytoconstituents were identified through a literature survey and from several databases. The pharmacokinetics study revealed that 7 phytoconstituents - glycyrrhisoflavone, eugenol, ferulic acid, methyl jasmonate, geranyl formate, formononetin, and elemicin- exhibited favourable pharmacokinetic properties. Five targets, HMOX1, CNR1, STAT3, HDAC2, and MAOB were found to be important in the network of 3300 proteins based on degree centrality and betweenness centrality. Among the seven phytoconstituents, glycyrrhisoflavone exhibited good dock scores and free energy value. Based on this, the stability of glycyrrhisoflavone with the five selected targets were analyzed using molecular dynamics study. Glycyrrhisoflavone showed good stability with most of the selected therapeutic targets. The current study reveals that the selected phytoconstituents i.e glycyrrhisoflavone, eugenol, ferulic acid, methyl jasmonate, geranyl formate, formononetin, and elemicin could serve as good lead molecules in treatment and management of Alzheimer’s disease through modulation of multiple targets.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"12 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000123/pdfft?md5=b4cdff257164ce9133a94cc20e16a137&pid=1-s2.0-S2590124923000123-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roasted cashew nut supplement inhibits MCP-1 and inflammatory mediators to correct hypertension related liver failure induced by mixed-fractionated petroleum products via NOS-cAMP-PKA signaling pathway in male rats","authors":"","doi":"10.1016/j.meomic.2023.100030","DOIUrl":"10.1016/j.meomic.2023.100030","url":null,"abstract":"<div><p>People who expose to mixture of crude oil products had been reported to show several diseased-symptoms including hypertension, liver failure and chronic complications. The drugs for treating multiple-complex diseases are scarcely available. Cashew nut snack is known to check multiple diseases and easily accessible by indigenous-patients with no lethal-effects. Here, we examined the corrective-measures of roasted-cashew-nut (RCN) against hypertension co-morbidity with liver-failure in male rats on exposure to mixture of fractionated-petroleum-products (MFPP). Seventy (70) male-albino-rats (n = 10) were randomly exposed to MFPP for 14 days. Group 1: control rats were given basal-diet. Group 2 was given basal diet + 0.2 ml/day-MFPP. Group 3 was given 0.2 ml/day-MFPP + 50 mg/kg Atenolol. Group 4 was given 0.2 ml/day-MFPP + 10 % RCN. Group 5 was given 0.2 ml/day-MFPP + 20 % RCN. Group 6 was given 10 % RCN_. Group 7 was given 20 % RCN. We found that high activities of liver-arginase, MAO-A, AChE, ADA, PDE-5¹ and ATP-hydrolytic-enzymes with low-bioavailability of NO-level on exposure to MFPP implicated liver-failure and hypertension. Also, up-regulation of HIF-1, p53, TNF-α, and MCP-1 with reduced-level of 1L-10 were connected to liver-failure and hypertension. However, post-treatment with 10 % RCN and 20 % RCN for 14-days corrected liver-failure and hypertension by inhibiting liver-arginase, MAO-A, AChE, ADA, PDE-5¹ and ATP-hydrolytic-enzymes. Also, liver-failure characterized by vascular-congestion and sinusoidal-dilation on exposure to 0.2 ml/day-MFPP was reversed by 10 % RCN and 20 % RCN via down-regulation of liver HIF-1, p53, TNF-α, MCP-1 with increased 1L-10. We conclude that 10 % RCN and 20 % RCN may be an innovative-snacks against hepatocellular damage co-morbidity with hypertension.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"12 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000111/pdfft?md5=0149f23ad6c9a902d3efb155b11fdefe&pid=1-s2.0-S2590124923000111-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138616682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological and histomorphometric evaluation of the therapeutic role of sulforaphane on the hypothalamic-pituitary-ovarian axis in polycystic ovary syndrome rats via its potential anti-inflammatory and anti-oxidant activities","authors":"Sunday Aderemi Adelakun ,&nbsp;Ogunlade Babatunde ,&nbsp;Chukwunenye Chidi Chinazo ,&nbsp;Olukayode Abimbola Arowosegbe ,&nbsp;Damilola Obanijesu Adisa","doi":"10.1016/j.meomic.2023.100029","DOIUrl":"10.1016/j.meomic.2023.100029","url":null,"abstract":"<div><p>Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism and insulin resistance. Sulforaphane (SFN) is a widely distributed isothiocyanate found in cruciferous vegetables. This study investigated the role of SFN on the hypothalamic-pituitary-ovarian axis in PCOS rats. Twenty-four female rats randomized into group A control received 2 ml of normal saline. Group B received 4 mg/kg body weight (bwt) I.P of estradiol valerate (EV) on the first day, groups C received 100 mg/kg bwt of SFN orally, and Group D received I.P 4 mg/kg bwt of EV follow by 100 mg/kg bwt of SFN orally. All administrations were done once daily for 30 days. Final body weight, estrous cycle, ovarian weight, blood glucose, serum sex hormones levels, serum lipid profile, ovarian antioxidants assay, ovarian inflammatory cytokines, and ovary and pituitary histology were assessed.</p><p>The PCOS rats exhibited the characteristic features of PCOS. Disturbed ovarian cyclicity, histopathological alterations, decreased the number of healthy follicles and corpora lutea and increased degenerated and cystic follicles were detected by light microscopic studies. A significant increase in final body weight, ovarian weight, blood glucose, sex steroids hormone levels, and antioxidant assays in PCOS rats. Sulforaphane reduces the elevated level of serum sex steroids hormone, lipid profile, ovarian diameter, and cysts and restores healthy follicles in PCOS rats. SFN reduces the upraised levels of ovarian oxidant and inflammatory cytokines and increases the suppressed antioxidant enzymes.</p><p>Sulforaphane thus ameliorates the disturbed hormonal levels, lipid profile, and antioxidant status in PCOS rats through its antioxidant and anti-inflammatory capacity.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259012492300010X/pdfft?md5=0287a765b122edb77d6f064071b38b83&pid=1-s2.0-S259012492300010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136092829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1H NMR metabolomics techniques demonstrated the effectiveness of methoxy substituted 2-benzylidene-1-indanone, A1 and A2A Adenosine Receptor antagonists, in modulating glucose production in the liver of diabetic rats","authors":"Olakunle Sanni ,&nbsp;David D. N'Da ,&nbsp;Jeremie Z. Lindeque ,&nbsp;Gisella. Terre'Blanche","doi":"10.1016/j.meomic.2023.100028","DOIUrl":"10.1016/j.meomic.2023.100028","url":null,"abstract":"<div><p>The physiological function of the liver in the overall disposal of postprandial glucose is important in the management of diabetes. Our recent research showed that benzylidene indanone derivative 2-(3,4-dihydroxybenzylidene)-4-methoxy-2,3-dihydro-1H-inden-1-one (2-BI) showed antihyperglycemic activity, but its role in glucose homeostasis in the liver is unknown. ¹H NMR metabolomics approach was used to unravel the effectiveness of 2-BI, on hepatic glucose production in fructose-streptozotocin (STZ) diabetics. Diabetes was induced in Sprague–Dawley rats using fructose-streptozotocin. Metabolites were extracted from the liver tissue and analyzed by ¹H NMR spectroscopy. Pathway analysis was performed on the identified metabolites. The unsupervised principal components analysis score plot showed clear differentiation. The control group (NC) was clearly separated from the diabetic group (DBC) and was clustered near the treated diabetic groups (DBI and DGT). There is a significant (p &lt; 0.01) increased level of 12 metabolites in diabetes rats that are crucial in liver glucose homeostasis. Treatment with 2-BI was able to lower the level of these metabolites. Detailed pathway analysis of the spiked metabolite levels shows an effect on the glycolysis/gluconeogenesis, butanoate, amino acid, nitrogen, and nucleotide (pyrimidine) metabolism. 2-BI reduced hyperglycemia in diabetic rats via the attenuation of hepatic glucose production and advancement of liver insulin sensitivity.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000093/pdfft?md5=bb8dbc5470a647fbf9b8f716cb174fba&pid=1-s2.0-S2590124923000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136059260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inferring molecular mechanisms of host-microbe-drug interactions in the human gastrointestinal tract","authors":"B. Roja,&nbsp;S. Saranya,&nbsp;L. Thamanna,&nbsp;P. Chellapandi","doi":"10.1016/j.meomic.2023.100027","DOIUrl":"10.1016/j.meomic.2023.100027","url":null,"abstract":"<div><p>This study aimed to elucidate the metabolic interplay between food-borne bacteria, gut methanogens, and probiotic bacteria using the host-microbe-drug interactome. This study also aimed to identify suitable drug combinations that could effectively combat food-borne infections without adversely affecting the normal gut microbes. In this study, the system medicine framework comprised 2654 edges and 1609 nodes, with 1370 interacting human genes. Through network modeling analysis, we identified interactions among 39 human target genes for food-borne bacteria, 11 targets for gut methanogens, and nine targets for probiotic bacteria. Gut methanogens target common human genes for their pathophysiological functions. Linoleic acid has emerged as a crosstalk metabolite that determines the abundance of foodborne bacteria. Gut microbes commonly share butyric acid, CO₃, and formaldehyde as metabolic precursors. Most antibiotics interact with human genes that target the gut methanogens and probiotic bacteria. Our study identified fusidic acid, nabiximol, oxacillin, ampicillin, phenoxymethylpenicillin, and cefdinir as repurposable antibiotics against foodborne bacterial infections. Chloroquine, an antimalarial drug, has been suggested as a potential repurposable drug for foodborne infections, owing to its indirect association with many foodborne bacteria via host-mediated interactions. Thus, our system medicine framework could potentially aid in suggesting repurposable antibiotics against food-borne bacterial infections, without affecting beneficial microbes in the human gastrointestinal tract.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590124923000081/pdfft?md5=94bb8f9882f44d3d6830b27127295383&pid=1-s2.0-S2590124923000081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136056282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic insights into heart development and cardiovascular diseases","authors":"Sona Charles ,&nbsp;Jeyakumar Natarajan","doi":"10.1016/j.meomic.2023.100026","DOIUrl":"10.1016/j.meomic.2023.100026","url":null,"abstract":"<div><p>The heart is a complex organ that starts to function by the end of the third week of gestation. Cardiovascular diseases attribute to the leading causes of death globally. The explosion of technological advances and computational tools for the analysis of next generation sequencing data has opened new avenues for comprehending the underlying regulatory mechanisms of complex disorders. The transcriptomic and genomic aspects of genes, non-coding RNAs and transcription factors have largely assisted understanding cardiogenesis and cardiovascular disease. The widespread application of transcriptomic methods has assisted in the screening of huge number of differentially expressed non-coding RNAs and their projected role as therapeutic targets and biomarkers. Transcriptomic profiling, including techniques such as RNA sequencing (RNA-Seq) has enabled the comprehensive characterization of gene expression patterns at various stages of embryonic and postnatal heart development. In the context of cardiac diseases, transcriptomic analyses have provided unprecedented insights into the molecular alterations associated with conditions such as myocardial infarction, heart failure, arrhythmias, and congenital heart defects. This review presents an overview of recent advancements in transcriptomic research aimed at unraveling the intricate genetic and molecular networks governing heart development, as well as in cardiac diseases. In this review, we attempted to systematically review the transcriptomic experiments performed to identify significant genes, miRNAs and lncRNAs with biomarkers therapeutic potential, <em>meta</em>-analysis of multiple samples, and ceRNA networks in heart diseases.</p></div>","PeriodicalId":100914,"journal":{"name":"Medicine in Omics","volume":"10 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259012492300007X/pdfft?md5=75604034cce55490ebdf16d0530d043d&pid=1-s2.0-S259012492300007X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}