MedComm – Oncology最新文献_第8页

Big data and single-cell sequencing in acute myeloid leukemia research 大数据和单细胞测序在急性粒细胞白血病研究中的应用

MedComm – Oncology Pub Date : 2023-08-28 DOI: 10.1002/mog2.47

Yuxuan Zou, Huiyuan Zhang, Hongbo Hu

{"title":"Big data and single-cell sequencing in acute myeloid leukemia research","authors":"Yuxuan Zou, Huiyuan Zhang, Hongbo Hu","doi":"10.1002/mog2.47","DOIUrl":"https://doi.org/10.1002/mog2.47","url":null,"abstract":"The advancement of diverse technologies has led to a substantial increase in valuable biomedical data, particularly in the field of acute myeloid leukemia (AML). Effective utilization of this wealth of data is crucial for attaining a comprehensive and in-depth understanding of AML, thereby facilitating optimal diagnosis, treatment, and prognosis. Among the various approaches to data acquisition, single-cell sequencing has emerged as an impressive tool. The developments of single-cell sequencing methods have empowered researchers to analyze the genome, transcriptome, proteome, and epigenome data at the single-cell level. It also offers a means to uncover fine information, providing unique prognostic insights and aiding in the identification of therapeutic targets. Furthermore, it enhances our understanding of AML heterogeneity, clonal evolution, and resistance mechanisms, ultimately leading to the development of better treatment strategies. In this review, we present an overview of AML as well as single-cell sequencing technologies, then explore their potential contributions to AML research in different aspects, and provide some information about resources and data processing.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.47","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50146476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cGAS-STING signaling in cancer: Regulation and therapeutic targeting 癌症中的cGAS-STING信号：调节和治疗靶向

MedComm – Oncology Pub Date : 2023-08-21 DOI: 10.1002/mog2.49

Xinzou Fan, Xiaoshuang Song, Wenjing Chen, Hantian Liang, Hiroko Nakatsukasa, Dunfang Zhang

{"title":"cGAS-STING signaling in cancer: Regulation and therapeutic targeting","authors":"Xinzou Fan, Xiaoshuang Song, Wenjing Chen, Hantian Liang, Hiroko Nakatsukasa, Dunfang Zhang","doi":"10.1002/mog2.49","DOIUrl":"https://doi.org/10.1002/mog2.49","url":null,"abstract":"Immunotherapy has revolutionized antitumor therapy. Since the discovery of stimulators of interferon genes (STING), efforts have been made to elucidate their mechanisms and physiological functions and explore the potential of STING as a therapeutic target in immune-related diseases and malignant tumors. In recent years, STING agonists have become a popular research topic. Activation of the cyclic GMP–AMP synthase (cGAS)-STING pathway produces large amounts of type I interferons, which play key roles in activating innate and acquired immune responses. The cGAS-STING pathway influences almost all aspects of tumorigenesis and has great antitumor potential. In addition, the activation of the cGAS-STING pathway is associated with tumor regression, prolonged survival of patients with cancer, and enhanced immunotherapy. Given the positive role of STING in antitumor immunity, the development of STING-targeted drugs is important. In this review, we summarize the activation and potential mechanisms of the cGAS-STING pathway, discuss the association of the cGAS-STING pathway with tumors and autoimmune diseases, and highlight research progress, clinical applications, and combination drug strategies for STING agonists.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50139724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule-targeting agents for cancer treatment: Seven binding sites and three strategies 用于癌症治疗的微管靶向剂：七个结合位点和三种策略

MedComm – Oncology Pub Date : 2023-08-16 DOI: 10.1002/mog2.46

Xingyu Wang, Benoît Gigant, Xi Zheng, Qiang Chen

{"title":"Microtubule-targeting agents for cancer treatment: Seven binding sites and three strategies","authors":"Xingyu Wang, Benoît Gigant, Xi Zheng, Qiang Chen","doi":"10.1002/mog2.46","DOIUrl":"https://doi.org/10.1002/mog2.46","url":null,"abstract":"Microtubules are pivotal in diverse cellular functions encompassing cell signaling, morphology, intracellular trafficking, and cell mitosis/division. They are validated targets for disease treatment, notably hematological cancers and solid tumors. Microtubule-targeting agents (MTAs) exert their effects by modulating microtubule dynamics, impeding cell proliferation, and promoting cell death. Recent advances in structural biology have unveiled novel perspectives for investigating multiple binding sites and mechanisms of action used by MTAs. In this review, we first provide an overview of the intricate structure and dynamics of microtubules. Then we explore the seven binding sites and the three primary strategies (stabilization, destabilization, and degradation) harnessed by MTAs. Furthermore, we introduce the emerging domain of microtubule-targeting degraders, exemplified by PROteolysis TArgeting Chimeras and small-molecule degraders, which enable precise degradation of specific microtubule-associated proteins implicated in cancer pathogenesis. Additionally, we discuss the promising realm of precision-targeted approaches, including antibody–drug conjugates and the utilization of photopharmacology in MTAs. Lastly, we provide a comprehensive overview of the clinical applications of microtubule-targeting therapies, assessing their efficacy and current challenges. We aim to provide a global picture of MTAs development as well as insights into the microtubule-targeting drug discovery for cancer treatment.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.46","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Billroth II with Braun and Roux-en-Y reconstructions after distal gastrectomy for gastric cancer: A meta-analysis 癌症远端胃切除术后Billroth II与Braun和Roux-en-Y重建术的比较：荟萃分析

MedComm – Oncology Pub Date : 2023-08-09 DOI: 10.1002/mog2.48

Defei Chen, Chenglin Tang, Fan He, Fuyu Yang, Saed Woraikat, Kun Qian

{"title":"Comparison of Billroth II with Braun and Roux-en-Y reconstructions after distal gastrectomy for gastric cancer: A meta-analysis","authors":"Defei Chen, Chenglin Tang, Fan He, Fuyu Yang, Saed Woraikat, Kun Qian","doi":"10.1002/mog2.48","DOIUrl":"https://doi.org/10.1002/mog2.48","url":null,"abstract":"Roux-en-Y (RY) and Billroth II with Braun (BB) reconstruction are two similar methods of reconstruction after distal gastrectomy (DG). Currently, it is unclear which method is superior. This meta-analysis was performed to compare the safety and efficacy of BB and RY reconstruction after DG for gastric cancer. A literature search of Pubmed, Embase, Web of Science, and Cochrane Library was conducted to identify studies comparing BB with RY after DG for gastric cancer until the end of October 2022. Main outcomes assessed were perioperative outcomes, postoperative complications, functional findings, and nutritional status. Meta-analyses were performed using RevMan 5.4 software. Finally, eight studies with a total of 910 patients were considered for the meta-analysis. The meta-analysis results revealed that operation time, anastomosis time, incidence of total complications, and delayed gastric emptying were reduced, and the incidence of bile reflux was increased in patients undergoing BB compared to RY reconstruction. In conclusion, BB has the advantage of reducing operative time, anastomotic time, intraoperative blood loss, overall postoperative complications, and delayed gastric emptying. RY has the advantage of preventing bile reflux and gastritis after surgery.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.48","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50142080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracorporeal bladder vibration assay enables early detection and recurrence monitoring for bladder cancer 体外膀胱振动检测可实现癌症的早期检测和复发监测

MedComm – Oncology Pub Date : 2023-07-23 DOI: 10.1002/mog2.45

Junqi Cui, Xia Wang, Jiali Lin, Yun Zou, Hongkang Wang, Wenyan Jiang, Jiajia Chen, Yamin Rao, Bin Xu, Long Li

{"title":"Extracorporeal bladder vibration assay enables early detection and recurrence monitoring for bladder cancer","authors":"Junqi Cui, Xia Wang, Jiali Lin, Yun Zou, Hongkang Wang, Wenyan Jiang, Jiajia Chen, Yamin Rao, Bin Xu, Long Li","doi":"10.1002/mog2.45","DOIUrl":"https://doi.org/10.1002/mog2.45","url":null,"abstract":"Cystoscopy is the current gold standard for diagnosing bladder cancer (Bca), but its invasive nature often results in patient discomfort. This study aims to debise a new strategy to enhance BCa detection. We collected data from 30 BCa patients between January and June 2022. We obtained spontaneously voided urine specimens from these patients before and after administering extracorporeal bladder vibration. These specimens underwent routine cytologic examination and fluorescence in situ hybridization (FISH). Furthermore, we conducted a follow-up 3 months postoperation. We recollected urine specimens before and after extracorporeal bladder vibration, repeating the cytologic examination and FISH. Our findings indicated an increase in the number of exfoliated cells in patients' urine postvibration compared to previbration. The liquid-based cell staining results showed an increased detection rate of abnormal cells in the urinary sediment of patients with both low and high-grade urothelial carcinoma postvibration. Similarly, the FISH results revealed an increased detection rate of CEP3 and CEP7 positive cells postvibration. Additionally, 3 months postoperation, we found abnormal cells in the urine of one patient previbration and in three patients postvibration. Further cystoscopic biopsy confirmed that these three patients had developed tumor recurrence. Our study substantiates that the extracorporeal bladder vibration assay significantly enhances BCa detection and the prediction of tumor recurrence. This method is simple, quick, and cost-effective, making it a promising approach worthy of widespread clinical application.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.45","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50153629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PTK2 promotes uveal melanoma metastasis by activating epithelial-to-mesenchymal transition PTK2通过激活上皮-间质转化促进葡萄膜黑色素瘤转移

MedComm – Oncology Pub Date : 2023-07-18 DOI: 10.1002/mog2.44

Pu Luo, Jingjing Duan, Qiong Chen, Ling Shao, Wen Xiao, Xunqi Liu, Gengwei Zhang, Xiaohua Tan, Zhongyi Fan

{"title":"PTK2 promotes uveal melanoma metastasis by activating epithelial-to-mesenchymal transition","authors":"Pu Luo, Jingjing Duan, Qiong Chen, Ling Shao, Wen Xiao, Xunqi Liu, Gengwei Zhang, Xiaohua Tan, Zhongyi Fan","doi":"10.1002/mog2.44","DOIUrl":"https://doi.org/10.1002/mog2.44","url":null,"abstract":"Uveal melanoma (UM) is the most common primary intraocular malignant tumor in adults. More than half of UM cases develop distant metastasis to the liver, lung, bone, and other organs, which frequently results in patient death. However, the mechanisms underlying UM metastasis remain largely unknown. Here, we report that protein tyrosine kinase 2 (PTK2), a nonreceptor protein tyrosine kinase also known as focal adhesion kinase (FAK), was overexpressed in most examined UM specimens. Furthermore, we identified PTK2 expression as a novel independent risk factor that predicts poor prognosis of UM patients. Mechanistic studies revealed that PTK2 promotes the EMT phenotype, leading to metastasis of UM cells. We showed that PTK2, located on chromosome 8q, is a functional gene of chromosome 8q gain to UM metastasis, which may represent the molecular mechanism for the aberrant expression and activation of PTK2 in UM. Our data reveal a novel role and mechanism of PTK2 in the metastatic process of UM, suggesting PTK2 may be a potential prognostic biomarker for UM metastasis and a promising therapeutic target for UM treatment.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.44","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50136893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of m1A modification gene polymorphisms with glioma risk in Chinese children m1A修饰基因多态性与中国儿童脑胶质瘤风险的关系

MedComm – Oncology Pub Date : 2023-07-16 DOI: 10.1002/mog2.43

Fan Liao, Rui-Xi Hua, Xingyu Jia, Yuxiang Liao, Li Yuan, Jichen Ruan, Tianfeng Li, Zhenjian Zhuo, Jing He

{"title":"Association of m1A modification gene polymorphisms with glioma risk in Chinese children","authors":"Fan Liao, Rui-Xi Hua, Xingyu Jia, Yuxiang Liao, Li Yuan, Jichen Ruan, Tianfeng Li, Zhenjian Zhuo, Jing He","doi":"10.1002/mog2.43","DOIUrl":"https://doi.org/10.1002/mog2.43","url":null,"abstract":"Glioma is a highly heterogeneous malignancy with a high mortality rate and poor prognosis. m1A methylation modifications are associated with gliomagenesis. However, whether single nucleotide polymorphisms (SNPs) of m1A modification genes are associated with glioma risk is unclear. We successfully genotyped 20 SNPs of m1A-modified genes TRMT10C, TRMT61B, TRMT6, TRM61, ALKBH1, YTHDC1, YTHDF1, and YTHDF2 in 314 pediatric glioma patients and 380 cancer-free controls using TaqMan probes. Associations of polymorphisms with glioma risk were assessed by the odds ratios and 95% confidence intervals generated by logistic regression models. Stratified analysis was performed by age, gender, tumor subtype, and clinical stage. The results showed that TRMT10C rs2303476, TRMT10C rs4257518, TRM61 rs2296484, and YTHDF2 rs3738067 polymorphisms were significantly associated with an increased risk of glioma, TRMT61B rs4563180, YTHDC1 rs2293595, and YTHDC1 rs3813832 polymorphisms were significantly associated with a reduced risk of glioma. In addition, analysis of the expression quantitative trait loci-showed that the TRM61 rs2296484 T allele significantly increased TRM61 messenger RNA (mRNA) expression, the YTHDF2 rs3738067 G allele significantly increased YTHDF2 mRNA expression, and the TRMT61B rs4563180 C allele significantly decreased TRMT61B mRNA expression. Overall, we identified several promising candidates for m1A modification gene polymorphisms as biomarkers of glioma risk.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.43","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50134566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of cuproptosis-related gene expression and positive correlations with CD4+ T cell infiltration in head and neck squamous cell carcinoma 头颈部鳞状细胞癌铜中毒相关基因表达及与CD4+T细胞浸润的相关性综合分析

MedComm – Oncology Pub Date : 2023-07-07 DOI: 10.1002/mog2.42

Zhangwei Hu, Wenbin Lei, Weiping Wen

{"title":"Comprehensive analysis of cuproptosis-related gene expression and positive correlations with CD4+ T cell infiltration in head and neck squamous cell carcinoma","authors":"Zhangwei Hu, Wenbin Lei, Weiping Wen","doi":"10.1002/mog2.42","DOIUrl":"https://doi.org/10.1002/mog2.42","url":null,"abstract":"Cuproptosis is a novel form of copper-induced programmed cell death. Here, we investigate the role of cuproptosis-related genes in head and neck squamous cell carcinoma (HNSCC), and the correlations between cuproptosis-related genes and tumor-infiltrating immune cells in the microenvironment. Gene expression data were downloaded from TCGA and analyzed using R. Protein–protein interaction analysis was conducted using STRING and GeneMANIA, while survival analyses examined the correlations between genes and overall survival. In addition, analyses of methylation and mutation sites were performed using Illumina HumanMethylation450 data and the cBioPortal data set, respectively. The correlations between gene expression level and infiltrating immune cells and predictive values of PD-1/PD-L1 blockade responses were also analyzed. Cuproptosis-promoting genes were downregulated, while two out of three cuproptosis-inhibiting genes, GLS and CDKN2A, showed upregulated expression. The CDKN2A expression level was identified as an independent prognostic factor for HNSCC through the multivariate Cox survival analysis. Meanwhile, levels of all cuproptosis-related genes correlated positively with CD4+ T cell infiltration. Furthermore, expression of the cuproptosis-promoting gene DLD was negatively correlated with immune score. All cuproptosis-related genes were expressed aberrantly and correlated positively with CD4+ T cell infiltration in HNSCC. Thus, copper homeostasis and cuproptosis could be targeted therapeutically as a potential means of enhancing the efficacy of immunotherapy.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.42","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50123771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sertindole inhibits autophagic flux and glioma progression Sertindole抑制自噬流量和神经胶质瘤进展

MedComm – Oncology Pub Date : 2023-06-26 DOI: 10.1002/mog2.41

Baiyang Liu, Yulin Shi, Bo Qin, Dating Cheng, Zhi Nie, Haoqing Zhai, Yao Li, Zhixian Jin, Jun Pu, Dongming Yan, Yongbin Chen, Cuiping Yang

{"title":"Sertindole inhibits autophagic flux and glioma progression","authors":"Baiyang Liu, Yulin Shi, Bo Qin, Dating Cheng, Zhi Nie, Haoqing Zhai, Yao Li, Zhixian Jin, Jun Pu, Dongming Yan, Yongbin Chen, Cuiping Yang","doi":"10.1002/mog2.41","DOIUrl":"https://doi.org/10.1002/mog2.41","url":null,"abstract":"Gliomas, the most lethal brain tumors, often exhibit resistance to conventional chemotherapy and/or radiotherapy. This study reveals that sertindole, a potent dopamine D2 receptor antagonist primarily designed as an antipsychotic medication for schizophrenia, effectively inhibits glioma progression. Our findings demonstrate that sertindole suppresses the proliferation of U251 and U87 tumor cells, impedes cell cycle progression in vitro, and curtails xenograft tumor growth in vivo. Moreover, we present compelling evidence demonstrating the ability of sertindole to enhance the cellular response to the chemotherapeutic agent temozolomide both in vitro and in vivo. Additionally, our findings reveal that sertindole effectively suppresses the self-renewal capacity and expression of stemness-associated genes, such as Nanog and Sox2, in glioma tumor cells and glioma stem cells. A mechanistic investigation demonstrated that sertindole enhances the formation of autophagosome–lysosome complexes while concurrently impeding autophagic flux through the inhibition of lysosomal hydrolytic enzymes CTSD and CTSB, ultimately resulting in decreased growth of tumor cells. In conclusion, our findings suggest that sertindole has the potential to develop into a potent antiglioma therapeutic agent.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.41","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50144909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA circGPRC5A(e2) facilitates gastric cancer progression and metastasis via modulating miR-665/LASP1 and activating PI3K/AKT pathway 环状RNA circGPRC5A（e2）通过调节miR-665/LASP1和激活PI3K/AKT途径促进癌症的进展和转移

MedComm – Oncology Pub Date : 2023-06-20 DOI: 10.1002/mog2.39

Yixun Lu, Guoxiao Liu, Yanjun Wang, Kai Li, Xinxin Xu, Benlong Zhang, Lin Chen, Hongqing Xi, Xinxin Wang

{"title":"Circular RNA circGPRC5A(e2) facilitates gastric cancer progression and metastasis via modulating miR-665/LASP1 and activating PI3K/AKT pathway","authors":"Yixun Lu, Guoxiao Liu, Yanjun Wang, Kai Li, Xinxin Xu, Benlong Zhang, Lin Chen, Hongqing Xi, Xinxin Wang","doi":"10.1002/mog2.39","DOIUrl":"https://doi.org/10.1002/mog2.39","url":null,"abstract":"Gastric cancer (GC) is one of the most commonly diagnosed malignancies worldwide. Compelling evidence indicates that circular RNA (circRNA) played critical roles in multiple cancers. However, the role and mechanisms of circRNAs in GC remains unclear. Here we first identified a notably overexpressed circular RNA hsa_circ_0025506 in GC by human circRNA microarray, designated as circGPRC5A(e2). Next, we found circGPRC5A(e2) was overexpressed in GC cell lines and clinical samples as well. Then, we confirmed that circGPRC5A(e2) was primarily located in the cytoplasm of GC cells and colocation phenomenon was observed with miR-665 via fluorescence in situ hybridization. Functionally, Cell Counting Kit-8, 5-Ethynyl-2′-deoxyuridine, clone formation assay, Transwell invasion assay, wound-healing assay, and animal experiments showed that circGPRC5A(e2) promoted GC proliferation, migration, and invasion in vitro, and tumorigenesis and metastasis in vivo. Mechanistically, we showed that circGPRC5A(e2) could serve as miR-665 sponges and facilitate GC growth and metastasis via modulating miR-665/LIM and SH3 protein 1 (LASP1) axis and activating phosphatidylinositol 3-kinase/AKT pathway. Taken together, this study revealed that circGPRC5A(e2) functioned as an oncogene in GC. The circGPRC5A(e2)/miR-665/LASP1 axis revealed by current research might provide novel biomarkers and promising therapeutic targets for GC.","PeriodicalId":100902,"journal":{"name":"MedComm – Oncology","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mog2.39","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50138931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0