中华微生物学和免疫学杂志最新文献

{"title":"Immunogenicity of quadrivalence recombinant human papillomavirus vaccine (6, 11, 16 and 18 types) (Hansenulapolymorpha): results from phaseI clinical trial","authors":"Yun-Jung Kang, Q. Lu, Ge Qu, Z. Jing, Chengfeng Zhao, Lifang Du, Junkai Liu, Qiang Liu, J. Nie, Y. Bai, Fengji Luo, Qiming Li","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.12.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.12.006","url":null,"abstract":"Objective \u0000To make a preliminary assessment on the immunogenicity of a quadrivalence recombinant human papillomavirus (HPV) vaccine (6, 11, 16 and 18 types) (Hansenulapolymorpha) in healthy women aged 18-45 years in phaseⅠclinical study. \u0000 \u0000 \u0000Methods \u0000It was a single-center, double-blind, randomized, placebo-controlled phaseⅠ clinical study. Women aged 18-45 years were randomized (2∶1) to receive HPV vaccine (n=60) or placebo control (n=30) at months 0, 2 and 6. Antibodies against HPV6/11/16/18 were detected by pseudovirus-based neutralisation assay in serum samples collected at 0 d, 180 d and 210 d. Seroconversion rates and geometric mean titres (GMT) of antibodies against the four types of antigens were calculated. \u0000 \u0000 \u0000Results \u0000Seroconversion rates of the vaccination group at 180 d (before the third dose) and 210 d (one month after the third dose) were generally similar and between 85%-100% for all types of antibodies. The GMT of antibodies at one month after the last dose improved significantly compared with those before immunization. \u0000 \u0000 \u0000Conclusions \u0000These results showed that the HPV vaccine had good immunogenicity in the population of healthy women aged 18-45 years. Higher antibody titers were elicited by the vaccine compare with the tites before the first dose and in the placebo control group. \u0000 \u0000 \u0000Key words: \u0000Quadrivalence recombinant human papillomavirus vaccine (6, 11, 16 and 18 types) (Hansenulapolymorpha); Immunogenicity; Seroconversion rate; Geometric mean titer","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"916-920"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47106108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology analysis of hand, foot and mouth disease cases and genetic characteristics of coxsackievirus A6 in six prefectures/cities of Yunnan Province in 2018","authors":"Yong Zhang, Xiaoqing Fu, Bing-jun Tian, Wen Xu, Lili Jiang, Jianping Cun, Xiaofang Zhou","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.12.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.12.001","url":null,"abstract":"Objective \u0000To detect the enterovirus VP4 and VP1 genes in 510 stool samples collected from hand, foot and mouth disease (HFMD) cases and analyze the phylogenetic characteristics of the entire VP1 genes of coxsackievirus A6 (CV-A6) strains in six prefectures/cities of Yunnan Province in 2018. \u0000 \u0000 \u0000Methods \u0000Viral RNA was abstracted from the stool samples. VP4 gene sequences were amplified by RT-PCR and sequenced using the MD91/OL68-1 primer pair to identify viral genotypes. Whole VP1 gene sequences were amplified and sequenced using appropriate primer pairs. The whole VP1 gene sequences of CV-A6 reference strains were downloaded from GenBank. MEGA5.2 software was used to analyze the similarity in nucleotide and amino acid sequences between different strains and phylogenetic tree was constructed for analysis of genetic characteristics and molecular epidemiology. \u0000 \u0000 \u0000Results \u0000VP4 and VP1 gene sequences were obtained from 57 out of 510 stool samples with a positive rate of 11.17% (57/510). There were 43 CV-A6 (8.43%, 43/510), six CV-A10 (1.17%, 6/510), two enterovirus A71 (EV-A71, 0.39%, 2/510) and two CV-A9 (0.39%, 2/510) strains. The other four strains were CV-A4 (0.19%, 1/510), CV-A5 (0.19%, 1/510), CV-B1 (0.19%, 1/510) and E11 (0.19%, 1/510). The phylogenetic analysis showed that all 43 CV-A6 strains belonged to sub-genotype D3. \u0000 \u0000 \u0000Conclusions \u0000In the 510 HFMD samples, CV-A6 strains were mostly detected with a detection rate of 8.43% and accounted for 75.44% (43/57) of all isolates, followed by CV-A10 (1.17%, 6/510) and EV-A71 (0.39%, 2/510). There was a large HFMD outbreak mainly caused by CV-A6 in Yunnan Province in 2018. The outbreak was caused by CV-A6 of sub-genotype D3, as was the case with pervious outbreaks in China. \u0000 \u0000 \u0000Key words: \u0000Hand, foot and mouth disease; Etiology; Coxsackievirus A6; VP4 gene; VP1 gene; Genetic characteristics analysis","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"885-891"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49667846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Schlafen gene family on HBV replication","authors":"Binli Mao, Xingru Zhou, Sidie Pi","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.12.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.12.002","url":null,"abstract":"Objective \u0000To investigate the regulatory effects of interferon-stimulated gene Schlafen (SLFN) on hepatitis B virus (HBV) replication. \u0000 \u0000 \u0000Methods \u0000Firstly, HepG2 cells were treated with IFN-α at different concentrations for 48 h or the same concentration for different periods of time. Expression of SLFN family genes was detected by quantitative real-time PCR (q-PCR). Secondly, the expression of SLFN gene family at mRNA level in HBV-infected tumor tissues and non-tumor tissues recorded in The Cancer Genome Atlas (TCGA) database was compared using t test. Furthermore, changes in the HBV DNA levels after the interference of small interfering RNA (siRNA)-mediated knockdown and overexpression of SLFN5 and SLFN11 genes were analyzed by q-PCR, Western blot and Southern blot. \u0000 \u0000 \u0000Results \u0000Among the SLFN gene family, only SLFN5 expression was induced by IFN-α in a concentration-dependent manner. TCGA database analysis showed that the expression of both SLFN5 and SLFN11 in HBV-infected tumor tissues and non-tumor tissues was significantly different. In HepG2 cells, knocking down the expression of SLFN5 had no obvious effect on the levels of intracellular HBV DNA. It was observed that the level of intracellular HBV DNA increased 1.79 folds in Huh7 cells after knockdown of SLFN11 expression, while no significant change in HBc protein was detected. Conversely, overexpression of SLFN11 induced a 34.67% decrease in intracellular HBV DNA in HepG2 cells. \u0000 \u0000 \u0000Conclusions \u0000In HepG2 cells, SLFN5 expression was induced by IFN-α, but had no effect on HBV replication. However, SLFN11 could inhibit the HBV DNA replication in nucleocapsid. \u0000 \u0000 \u0000Key words: \u0000SLFN; IFN-α; Hepatitis B virus; Antivirus","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"892-897"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43924263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress in immunoregulatory effects of adipose mesenchymal stem cells","authors":"Huiqing Hu, X. Hou","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.12.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.12.012","url":null,"abstract":"Adipose derived mesenchymal stromal cells (ADSC) are pluripotent stem cells isolated from adipose tissue and have great application potential in regenerative medicine for their rich sources of derivation, relatively less trauma when sampling and few ethical problems. ADSC have preferable immunomodulatory capacity, and can regulate the immunologic functions of dendritic cells, macrophages, NK cells, NKT cells, B lymphocytes and T lymphocytes. In order to better understand the role of ADSC in immunoregulation, this paper systematically reviewed the immunomodulatory effects of ADSC on several representative immune cells. \u0000 \u0000 \u0000Key words: \u0000ADSC; Immunocyte; Immunoregulation","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"958-964"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48082531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing, virulence genes and drug resistance of Salmonella typhimurium in Longyan city","authors":"Qian-jin Chen, Meihua Li, Chunyuan Cao, L. Liao, Haibin Chen, Xiao-dong Chen","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.001","url":null,"abstract":"Objective \u0000To analyze the molecular typing characteristics by pulsed field gel electrophoresis (PFGE), drug resistance and virulence genes of Salmonella typhimurium in Longyan city in order to provide reference for the prevention and control. \u0000 \u0000 \u0000Methods \u0000A total of 79 Salmonella typhimurium strains were isolated from sporadic cases of diarrhea and food poisoning and raw poultry meat samples during 2010 to 2017. PFGE was performed to measure the minimum inhibitory concentrations (MIC) of 15 commonly used drugs against them. PCR was used to detect nine virulence genes (sopB, invA, sifA, sscA, sseE, spvB, pefA, spvR, spvC) in 55 strains. \u0000 \u0000 \u0000Results \u0000The 79 Salmonella typhimurium strains belonged to 61 PFGE types. There were 10, three and four strains of P1, P3 and P21 types, respectively. Seven P1 type strains were isolate from one food poisoning event. According to the 85% classification standard, 79 Salmonella typhimurium strains could be divided into five predominant gene clusters (G1-G5). Drug susceptibility test showed that the 79 strains had the highest resistance rate to ampicillin (88.61%), followed by that to tetracycline (87.34%) and streptomycin (73.41%). Multidrug resistant bacteria resistant to three or more antibacterial drugs accounted for 84.81% (67/79). All of the 55 strains carried invA, sopB, sseE and sscA genes. The other five genes, sifA, spvC, spvB, spvR and pefA, were detected in 54, 31. 10, 11 and 12 strains, respectively. There were 76.4% (42/55) of the strains carrying five or six virulence genes and all were positive for invA, sopB, sseE, sscA and sifA, and negative for spvB, spvR and pefA. The strains carrying all of the nine virulence genes accounted for 18.2% (10/55). \u0000 \u0000 \u0000Conclusions \u0000Salmonella typhimurium strains isolated in Longyan city had a diverse PFGE type. P1, P3 and P21 types were the three predominant PFGE types. In the food poisoning event, PFGE molecular typing could quickly alert the outbreak and traceability of Salmonella typhimurium. Attention should be paid to the multidrug resistance in Salmonella typhimurium. Monitoring of multidrug-resistant strains and supervision on antibacterial drug usage should be strengthened. Salmonella typhimurium had high virulence as it carried many virulence genes. \u0000 \u0000 \u0000Key words: \u0000Salmonella typhimurium; PFGE molecular typing; Cluster analysis; Minimum inhibitory concentration; Multidrug resistance; Virulence gene; Salmonella virulence island; Salmonella plasmid virulence gene","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"805-811"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44308928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiological characteristics of Streptococcus pyogenes causing scarlet fever and angina in children","authors":"Yinghua Zhang, Hui Yu, Xiao-guang Wang, Yingying He, Hongjing Yan","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.003","url":null,"abstract":"Objective \u0000To investigate the antimicrobial resistance, macrolide-resistance genes, virulence genes and emm types in Streptococcus pyogenes isolates. \u0000 \u0000 \u0000Methods \u0000A total of 247 oropharyngeal swab specimens were collected from pediatric outpatients (aged 2-11 years) who were clinically diagnosed as scarlet fever in Children′s Hospital of Fudan University from January to December, 2018. These specimens were timely sent to the Microbiology Laboratory for isolation and identification of Streptococcus pyogenes strains were isolate after culturing and identified with bacitracin susceptibility test. Moreover, the diameter of bacitracin inhibition zone was measured by vernier caliper. Their susceptibility to seven antibiotics, including erythromycin, clarithromycin, clindamycin, ampicillin, ceftriaxone, norfloxacin and chloramphenicol, were measured using KB method. Macrolide-resistance genes (mefA, ermA, ermB and Tn916 transposon) and virulence genes (speA, speB, speC, speG, speH, speI, speJ and speK) were detected by PCR. Amplification and sequencing of emm gene were conducted according to the protocol in the website of Centre for Disease Control and Prevention (CDC). \u0000 \u0000 \u0000Results \u0000A total of 86 strains of Streptococcus pyogenes were isolated from the 247 specimens. Their resistance rates to erythromycin, clarithromycin and clindamycin were 89.5%, 95.3% and 96.5%, respectively. However, these isolates showed high susceptibility to ampicillin (100.0%), ceftriaxone (100.0%), norfloxacin (90.7%) and chloramphenicol (95.3%). The positive rates of mefA, ermA, ermB and Tn916 genes were 20.9%, 24.4%, 98.8% and 97.7%. There was significant difference in the mefA-carrying rates between patients with scarlet fever and angina. The positive rates of virulence genes of speA, speB, speC, speG, speH, speI, speJ, speK, speL and speM were 8.1%, 100.0%, 95.3%, 100.0%, 80.2%, 90.7%, 10.5%, 100.0%, 5.8% and 5.8%. Seven emm types were identified and the predominant types were emm12.0 (75.6%), emm12.19 (9.3%) and emm1.0 (8.1%). The diameter of bacitracin inhibition zone was smaller in isolates of emm1.0 type than in emm12.0 type strains. The profile of virulence genes varied in the strains of different emm types. All types of strains carried speB, speG and speK genes. The isolates of emm1.0 type carried speA virulence gene, while speB, speC, speG, speH, speI and speK genes were more often identified in emm12.0 type isolates. \u0000 \u0000 \u0000Conclusions \u0000This study showed that emm types were associated with the profile of virulence genes and the diameter of bacitracin inhibition zone. It was recommended that the diameter of bacitracin inhibition zone should be measured in bacitracin inhibition susceptibility test apart from only observing the formation of inhibition zone. \u0000 \u0000 \u0000Key words: \u0000Streptococcus pyogenes; Antimicrobial resistance; Virulence gene; emm type; Bacitracin susceptibility test","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"821-826"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46813047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement and mechanism of fecal microbiota transplantation on endotoxic acute lung injury in rats","authors":"Bo Li, G. Yin","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.002","url":null,"abstract":"Objective To observe the effects of fecal microbiota transplantation (FMT) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats and to investigate the possible mechanism in order to provide new thoughts for the treatment of acute lung injury. Methods Forty-five adult healthy male SPF level SD rats were randomly divided into three groups of normal saline (NS), LPS and FMT with 15 in each group. ALI was induced by intraperitoneal injection of rats with 5 mg/kg of LPS. The FMT group was given 10 ml/kg of fecal microbiota solution intervention (twice a day for two consecutive days) after ALI induction. Five rats in each group were randomly selected and sacrificed at 24 h, 48 h and 72 h after intervention. Pathological changes in lung tissues were examined with hematoxylin and eosin (HE) staining and pathological scores were assessed. Right lung samples were weighed to measure wet/dry (W/D) ratios. Abdominal aorta blood samples were collected for PaO2 analysis. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6) in bronchoalveolar lavage fluid (BALF) were detected by enzyme linked immunosorbent assay (ELISA). Expression of transforming growth factor-β (TGF-β) and intracellular signal transduction protein Smads (Smad3 and Smad7) at mRNA level was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Expression of ERK1/2 and p-ERK1/2 at protein level was detected by Western blot (WB). Rat fecal samples were collected to extract DNA and the PCR products of V3 and V4 regions were sequenced by Illumina MiSeq. Bioinformatics analysis on microbiota was conducted with Illumina MiSeq based on operational taxonomic unit (OTU) clustering. Results Compared with the NS group, the LPS group showed significantly widened alveolar septa, massive inflammatory cell infiltration and some alveolar collapse in lung tissues. Compared with the LPS group, the FMT group showed significantly alleviated inflammatory cell infiltration, reduced swelling in pulmonary interstitial tissues, and less damage to pulmonary structure. Compared with the NS group, the W/D lung ratio, PaO2 concentration and TNF-α, IL-1 and IL-6 levels in BALF in the LPS group significantly increased at 24 h after intervention and then gradually decreased, but still higher than those in the NS group (P<0.05). The W/D lung ratio, PaO2 concentration and TNF-α, IL-1 and IL-6 levels in BALF of the FMT group were significantly lower than those of the LPS group (P<0.05). Compared with the NS group, the expression of TGF-β1 and Smad3 at mRNA level in lung tissues increased at all time points in the LPS group, while the expression of Smad7 at mRNA level decreased (P<0.05). The expression of TGF-β1 and Smad3 at mRNA level in the FMT group were significantly lower than that in the LPS group (P<0.05), while the expression of Smad7 at mRNA level was higher (P<0.05). Compared with the NS group, p-ERK expression significantly","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"812-820"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46849808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-emulsion as a vaccine adjuvant can enhance the humoral immunity against influenza in aged and young mice","authors":"Pu Shan, Zhibiao Wang, Duoqian Wei, Shaojie Hao, Dexiang Chen, Jing Xu","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.010","url":null,"abstract":"Objective \u0000To evaluate the immunogenicity of split influenza H1N1 vaccine formulated with an oil-in-water nano-emulsion adjuvant in aged mice and young mice. \u0000 \u0000 \u0000Methods \u0000A nano-emulsion adjuvant formulated split influenza H1N1 vaccine was used to immunize aged and young mice through intramuscular injection. Each mouse was immunized with 0.012 μg of hemagglutinin (HA) twice with an interval of 28 d. Hemagglutination inhibition (HI) titers in serum were measured 27 d after first immunization. Serum HI, IgG1 and IgG2a titers were detected 14 d after the last immunization. No adjuvant-formulated vaccine and normal saline (NS) were used to set up control groups. Virus challenge test was carried out using 10 times the median lethal dose (LD50) of A/Puerto Rico/8/34 (H1N1) strain two weeks after the last immunization and the protective effects were assessed through measuring the dynamic changes in body weight and survival rate. \u0000 \u0000 \u0000Results \u0000Higher levels of serum HI, IgG1 and IgG2a antibodies and higher HI antibody conversion rates were induced in the adjuvant groups, especially in the aged mice group, than in the control groups. Nano-emulsion adjuvant improved the immunogenicity of HA and mouse immunity to A/Puerto Rico/8/34 (H1N1). \u0000 \u0000 \u0000Conclusions \u0000Nano-emulsion adjuvant could enhance the immunogenicity of influenza antigens, especially in aged mice. \u0000 \u0000 \u0000Key words: \u0000Adjuvant; Influenza; Nano-emulsion adjuvant; Aged mice; Immunogenicity","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"869-874"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47980102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the detection and clearance of hepatitis B virus covalently closed circular DNA (cccDNA)","authors":"Yuan Tian","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.011","url":null,"abstract":"Chronic hepatitis B (CHB) is a worldwide public health problem and current therapeutic drugs cannot completely eliminate the covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) in the liver cell nucleus. The presence of cccDNA leads to the persistent HBV infection, which is hard to be completely eliminated. At present, the research on cccDNA is getting increasing attention. Only by comprehensively understanding it can we find a better way to cure HBV infection. This article reviewed the structure, regulation, detection and elimination of HBV cccDNA. \u0000 \u0000 \u0000Key words: \u0000HBV; cccDNA; Detection; Elimination","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"875-879"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48602256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of TET2-targeting miR-27b on oxidized low-density lipoprotein-induced inflammatory responses and apoptosis in endothelial cells","authors":"J. Bao, Bei Sun, P. Ma, Hui Xu, Lili Ji","doi":"10.3760/CMA.J.ISSN.0254-5101.2019.11.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-5101.2019.11.008","url":null,"abstract":"Objective \u0000To investigate the effects of miR-27b targeting ten-eleven translocation methylcytosine dioxygenase 2 (TET2) on oxidized low-density lipoprotein (ox-LDL) induced inflammatory responses and apoptosis in endothelial cells. \u0000 \u0000 \u0000Methods \u0000Double luciferase reporter gene analysis verified the targeting effect of miR-27b on TET2. Human umbilical vein endothelial cells (HUVECs) were induced by ox-LDL in vitro. Eight groups were set up including control group, ox-LDL group, ox-LDL+ anti-miR-con group, ox-LDL+ anti-miR-27b group, ox-LDL+ pcDNA group, ox-LDL+ pcDNA-TET2 group, anti-miR-27b+ si-con group and anti-miR-27b+ si-TET2 group. qRT-PCR was used to detect the expression of miR-27b and TET2 at mRNA level. Cell viability was detected by MTT assay. Cell apoptosis rate was detected by flow cytometry. Western blot was used to detect the expression of TET2, Cyclin D1 and caspase-3 at protein level. \u0000 \u0000 \u0000Results \u0000TET2 was the target gene of miR-27b. TET2 expression could be negatively regulated by miR-27b. ox-LDL increased the expression of miR-27b and reduced the expression of TET2 in HUVECs. The secretion of inflammatory factors and apoptosis rates of HUVECs in the control, ox-LDL+ anti-miR-27b, ox-LDL+ pcDNA-TET2 and anti-miR-27b+ si-con groups were significantly lower than those in the ox-LDL, ox-LDL+ anti-miR-con, ox-LDL+ pcDNA and anti-miR-27b+ si-TET2 groups, respectively (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000miR-27b promoted ox-LDL-induced inflammatory responses and apoptosis in endothelial cells through down-regulating the expression of TET2. \u0000 \u0000 \u0000Key words: \u0000miR-27b; ox-LDL; TET2; HUVEC; Inflammatory response; Apoptosis","PeriodicalId":10089,"journal":{"name":"Chinese journal of microbiology and immunology","volume":"39 1","pages":"856-863"},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44430046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}