Laboratory Robotics and Automation最新文献_第5页

Studies concerning the biomonitoring of the degree of environmental pollution using a urea-sensitive enzymatic sensor 脲敏感酶传感器对环境污染程度的生物监测研究

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<27::AID-LRA5>3.0.CO;2-U

Fiammetta Kormos, Adrienne Naumescu Lengauer

{"title":"Studies concerning the biomonitoring of the degree of environmental pollution using a urea-sensitive enzymatic sensor","authors":"Fiammetta Kormos, Adrienne Naumescu Lengauer","doi":"10.1002/(SICI)1098-2728(2000)12:1<27::AID-LRA5>3.0.CO;2-U","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<27::AID-LRA5>3.0.CO;2-U","url":null,"abstract":"The biosensor, which determines the variation of the pH as a consequence of the hydrolysis process of urea, is composed of a biological receiver (the urease) and a potentiometric sensor (a metallic pH electrode, tungsten). Keeping in mind that the sensitivity of the sensor changes depending with the inhibitor concentration for urease (which is present in the water or in the soil), this could be used to determine the degree of pollution of the surface waters and of the soil. In this way it would be possible to establish the quantities of the urea utilized as chemical fertilizer.In the present work, we present the investigation of the inhibition effect of the ions Pb2+, Fe2+, Sn2+, Zn2+ and Hg2+ toward the urea-sensitive biosensor. The determinations with samples of surface waters and soils in the presence of 10−1 M urea and one-minute contact time has demonstrated the possibility of monitoring the concentrations of these ions in the following domains: 10−2–10−6 M Fe2+; 10−2–10−6 M Zn2+; 10−4–10−6 M Pb2+. These limiting values are found if the cation is individually present. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:27–30, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<27::AID-LRA5>3.0.CO;2-U","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82405673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Slurry analysis by multichannel arc/spark emission spectrometry using a monosegmented system: Determination of aluminium in sediments 使用单节段系统的多通道电弧/火花发射光谱法进行浆液分析:沉积物中铝的测定

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<46::AID-LRA9>3.0.CO;2-J

C. E. C. Magalhães, C. Pasquini, M. A. Z. Arruda

{"title":"Slurry analysis by multichannel arc/spark emission spectrometry using a monosegmented system: Determination of aluminium in sediments","authors":"C. E. C. Magalhães, C. Pasquini, M. A. Z. Arruda","doi":"10.1002/(SICI)1098-2728(2000)12:1<46::AID-LRA9>3.0.CO;2-J","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<46::AID-LRA9>3.0.CO;2-J","url":null,"abstract":"This work proposes a new approach for sediment analysis in which the sample is introduced as a slurry in an arc/spark emission spectrometer using a monosegmented system. The spectrometer has been modified to employ a 1024 photodiode-array detector capable of collecting intensities in a 18.7 nm wide wavelength window. Sediment slurries were prepared with 8.5–50 mg of the samples, grounded to a particle size less than 38 μm, transferred to a 50 mL volumetric flask in which 1 mL of conc. HNO3 and 300 μL of 10% m/v La (as La2O3; used as internal standard) were added. The slurry (25 μL) was introduced into the monosegmented system and transported by an air stream to a graphite rod in the existing arc/spark source. About 40 samples can be analyzed per hour. Results for reference materials show acceptable accuracy and precision for the proposed method. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:46–52, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<46::AID-LRA9>3.0.CO;2-J","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80815852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Automatic multicommutated flow system for ethanol determination in alcoholic beverages by spectrophotometry 用分光光度法测定酒精饮料中乙醇的自动多换向流系统

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<31::AID-LRA6>3.0.CO;2-7

Ana Lucia D. Comitre, Boaventura F. Reis

{"title":"Automatic multicommutated flow system for ethanol determination in alcoholic beverages by spectrophotometry","authors":"Ana Lucia D. Comitre, Boaventura F. Reis","doi":"10.1002/(SICI)1098-2728(2000)12:1<31::AID-LRA6>3.0.CO;2-7","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<31::AID-LRA6>3.0.CO;2-7","url":null,"abstract":"In this work, we describe a automatic spectrophotometric procedure for ethanol determination in alcoholic beverages by employing a flow system based on a multicommutation and binary sampling approach. The method was based on ethanol oxidation by dichromate in sulfuric acid medium and detection by spectrophotometry. The procedure was applied to ethanol determination in sugar-cane spirit, whisky, and brandy. Accuracy was assessed by comparing the results with those obtained by densimetry for sugar-cane-spirit samples and by the standard-addition technique for whisky and brandy samples. In the first case, no significant difference at the 95% confidence level was observed and in the second case, recovery ranging from 95 to 109% was achieved. Other profitable features such as an analytical throughput of 40 determinations per hour, a relative standard deviation of 1.6% (n = 11) for a typical sample presenting 42.1% (v/v) ethanol, and a reagent consumption of 5.4 mg K2Cr2O7 per determination generating a low volume of waste per determination (3.3 mL) were also achieved. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:31–36, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<31::AID-LRA6>3.0.CO;2-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84915112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Linear relationship between potential and concentration in flow potentiometry 流动电位法中电位与浓度的线性关系

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<41::AID-LRA8>3.0.CO;2-0

José Anchieta Gomes Neto, Elias Ayres Guidetti Zagatto

{"title":"Linear relationship between potential and concentration in flow potentiometry","authors":"José Anchieta Gomes Neto, Elias Ayres Guidetti Zagatto","doi":"10.1002/(SICI)1098-2728(2000)12:1<41::AID-LRA8>3.0.CO;2-0","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<41::AID-LRA8>3.0.CO;2-0","url":null,"abstract":"In potentiometric-flow systems, linear-potential responses for logarithmic concentrations can be attained for first-(or pseudo-first-) order reactions in which the monitored chemical species react with the analyte during a fixed time interval. To demonstrate this property, the determination of glycerol based on its oxidation by periodate and potentiometric monitoring of the remaining periodate was selected. Influence of reagent concentration and timing on the linearity of the analytical curve were investigated. A mathematical treatment was derived, and potentialities/limitations of the approach were outlined. The system was applied to analysis of soap and lixivia samples. The analytical curve within 200 and 2000 mg L−1 (r = 0.99975; n = 5) was described as E = 8.166 + 0.0478 [glycerol]. The sample throughput was 100 h−1, and a measurement repeatability within 0.5 mV was always observed. By applying a t-test, there was no statistical difference between the results obtained by the proposed procedure and by iodimetric titration at the 95% confidence level. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:41–45, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"41-45"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<41::AID-LRA8>3.0.CO;2-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79193286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A computer-controlled battery-powered micropotentiostat 一种由计算机控制的电池供电的微型电位器

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<53::AID-LRA10>3.0.CO;2-U

Mario R. Romero, Juan M. Marioli

引用次数: 1

Design of a robotic workstation for automated organic synthesis 自动化有机合成机器人工作站的设计

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<2::AID-LRA2>3.0.CO;2-K

Hideho Okamoto, Kohji Deuchi

{"title":"Design of a robotic workstation for automated organic synthesis","authors":"Hideho Okamoto, Kohji Deuchi","doi":"10.1002/(SICI)1098-2728(2000)12:1<2::AID-LRA2>3.0.CO;2-K","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<2::AID-LRA2>3.0.CO;2-K","url":null,"abstract":"A robotic workstation for automated organic synthesis was developed. The system consists of a computer, robot, and several devices (reaction device, separation device, dispense device, etc.). The reaction device has four temperature-regulation units, each of which can freely change its set reaction temperature. Since each of the four can handle four reaction containers, 16 reactions under different conditions can be done simultaneously. The range of reaction temperature is from −30°C to 160°C. Reactions beyond the boiling points of solvents are possible by using reflux condensers. The separation processing device automatically performs solution extraction operations based on the detected liquid levels and interface positions. The dispensing device automatically pumps various kinds and amounts of reagents and/or solvents at various speeds into the reaction containers by switching valves through the digital syringe pump. This system can automate all synthesis and analysis process and can perform at least 3000 experiments (3000 containers) per year. Since its completion in 1996, it has conducted over 1500 experiments of various kinds. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:2–11, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"2-11"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<2::AID-LRA2>3.0.CO;2-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82162301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Editorial notes 社论指出

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<1::AID-LRA1>3.0.CO;2-Q

W. Jeffrey Hurst Ph.D., FAIC

引用次数: 0

On the DZ molecular descriptors 在DZ分子描述符上

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<37::AID-LRA7>3.0.CO;2-N

D. Zakarya, M. Nohair, H. Nyassi

引用次数: 0

A comprehensive and flexible approach to the automated-dissolution testing of pharmaceutical drug products incorporating direct UV–vis fiber-optic analysis, on-line fluorescence analysis, and off-line storage options 一种全面灵活的药物产品自动溶出测试方法，包括直接紫外-可见光纤分析、在线荧光分析和离线存储选项

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<12::AID-LRA3>3.0.CO;2-F

P. Rogers, P. A. Hailey, G. A. Johnson, V. A. Dight, C. Read, A. Shingler, P. Savage, T. Roche, J. Mondry

引用次数: 8

Study of hemolysis interference with a peak rate nephelometric assay of lipoprotein(a) 峰值速率浊度法测定脂蛋白溶血干扰的研究(a)

Laboratory Robotics and Automation Pub Date : 2000-02-25 DOI: 10.1002/(SICI)1098-2728(2000)12:1<23::AID-LRA4>3.0.CO;2-8

Fabrice Lefèvre, Philippe Gillery

{"title":"Study of hemolysis interference with a peak rate nephelometric assay of lipoprotein(a)","authors":"Fabrice Lefèvre, Philippe Gillery","doi":"10.1002/(SICI)1098-2728(2000)12:1<23::AID-LRA4>3.0.CO;2-8","DOIUrl":"10.1002/(SICI)1098-2728(2000)12:1<23::AID-LRA4>3.0.CO;2-8","url":null,"abstract":"Lipoprotein(a) [Lp(a)] is considered an independent risk factor in atherosclerosis and thrombosis. Its measurement in clinical laboratories necessitates the use of reliable methods and a strict control of preanalytical conditions. We studied here the potential interference of hemolysis on a peak rate nephelometric method. Serum samples were spiked with up to 500 μmol/L hemoglobin (Hb), and Lp(a) was measured with a Beckman Array 360 analyzer. Hb supplementation induced a significant decrease of Lp(a) values: −15% and −25% (p < 0.01) for Hb concentrations 200 and 500 μmol/L, respectively. Similar results were obtained when Hb addition was done before and after the manual step of sample predilution (1/5, v/v) in PEG-containing buffer. No significant difference of Hb interference was noticed according to apo(a) phenotypes. Our results indicate that hemolysis may significantly alter Lp(a) values obtained with this method, probably because of the low-dilution rate of samples. Even if this interference is of limited extent, it should be taken into account when interpretating results close to decisional values used for risk assessment. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:23–26, 2000","PeriodicalId":100863,"journal":{"name":"Laboratory Robotics and Automation","volume":"12 1","pages":"23-26"},"PeriodicalIF":0.0,"publicationDate":"2000-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1098-2728(2000)12:1<23::AID-LRA4>3.0.CO;2-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86618850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0