Journal of Pharmaceutical and Biomedical Analysis Open最新文献

{"title":"Turning diverse analytical data into actionable knowledge for enzymatically driven polysorbate degradation risk assessment and control in biotherapeutic protein formulations","authors":"Alex Dow ,&nbsp;Divya Chandra ,&nbsp;Ximeng Dow ,&nbsp;Benjamin Adams ,&nbsp;Morgan Ayres ,&nbsp;Shannon Rivera ,&nbsp;Rong-Sheng Yang ,&nbsp;Anita P. Liu ,&nbsp;Xuanwen Li ,&nbsp;Tingting Jiang ,&nbsp;Fengqiang Wang ,&nbsp;Tony Pereira ,&nbsp;Rosalind Ang ,&nbsp;Chunyan Li ,&nbsp;Lei Zhang ,&nbsp;Jonathan Welch ,&nbsp;Lloyd Breunig","doi":"10.1016/j.jpbao.2025.100068","DOIUrl":"10.1016/j.jpbao.2025.100068","url":null,"abstract":"<div><div>In recent years polysorbate (PS) degradation in biotherapeutic protein formulations has become one of the most challenging topics for residual host cell protein control. With such focus, various assays have been showcased to help inform on enzymatically driven PS degradation risk assessment and control. Access to multiple, orthogonal data sets can improve understanding but also increases complexity in data interpretation. To highlight how multiple assays can work together to provide an aligned enzymatically driven PS degradation risk assessment and control, three cases studies are discussed. The case studies are diverse in the driver for performing these experiments along with if they are proactively or reactively addressing PS degradation. From these three case studies it becomes apparent that assays are consistent in their use and alignment regarding enzymatically driven PS degradation risk assessment and control. In general, an assay will fall into one of three categories: risk informing, characterization, and extended characterization. With this information future work focused on enzymatically driven PS degradation risk assessment and control has a blueprint for what assays can be used and what the data informs on.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"5 ","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spotlights of MIP-sensors for drugs and protein biomarkers","authors":"Aysu Yarman ,&nbsp;Aysel Oktay ,&nbsp;Melis Işık Toksoy ,&nbsp;Sivoney Ferreira de Souza ,&nbsp;João Ameixa ,&nbsp;Ilko Bald ,&nbsp;Cem Bulent Ustundag ,&nbsp;Frieder W. Scheller","doi":"10.1016/j.jpbao.2024.100048","DOIUrl":"10.1016/j.jpbao.2024.100048","url":null,"abstract":"<div><div>Molecularly Imprinted Polymers (MIPs) are potential tools in pharmaceutical analysis and for determining protein biomarkers. This review presents a comprehensive comparison of MIP synthesis concepts, i.e., segment <em>vs.</em> whole template imprinting and polymerization of the template/monomer mixture <em>vs.</em> “hierarchical (oriented) imprinting” and combinations of MIPs with enzymes, antibodies, and aptamers, respectively. For low-molecular-weight substances such as drugs and biomacromolecules, the hierarchical polymer synthesis around the oriented template results in MIPs with higher affinity than MIPs prepared by polymerizing a mixture of the template and functional monomers. Application of the target molecule fragments as the template, so-called epitopes, gives MIPs that possess comparable affinity towards the whole analyte as the “Whole-molecule”-MIPs, but the synthesis costs are considerably lower. The combination of MIPs with enzymes, antibodies, and aptamers allows the expansion of the analyte spectrum, amplifies the signal, and suppresses interfering substances. Catalytically active MIPs may, in the future, substitute enzymes and catalyze unnatural reactions.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"5 ","pages":"Article 100048"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle-supported electrochemical sensors for pesticide analysis in fruit juices","authors":"Ensar Piskin ,&nbsp;Zeynep Alakus ,&nbsp;Fatma Budak ,&nbsp;Ahmet Cetinkaya ,&nbsp;Sibel A. Ozkan","doi":"10.1016/j.jpbao.2025.100056","DOIUrl":"10.1016/j.jpbao.2025.100056","url":null,"abstract":"<div><div>Pesticides, used to destroy insects, diseases, and weeds in agricultural products, have increased agricultural activities in countries in recent years. While they have helped reduce the number of pests affecting crops and improved agricultural yields, they have also caused significant environmental hazards. Their toxicity damages the habitats of beneficial creatures, wild animals, and the targeted pests. Additionally, pesticides have a lengthy half-life in the environment, which allows them to build up in species' tissues and go up the food chain, a process known as bioaccumulation and biomagnification. This is a danger to both ecological balance and human health. While a certain amount of excessively used pesticides has a positive effect, increasing amounts remain in the soil and environment as residues. Pesticide residues in the soil pass into foods and fruits. Therefore, there has been a critical need to design electrochemical sensors for sensitive, selective, and rapid analysis of pesticides. Electrochemical sensors offer economical, cost-effective, easy to apply, environmentally friendly, sensitive, and selective advantages for quality and reliable analysis of foods. In addition, many functional nanomaterials are used to increase these sensors' innovative designs and selective and sensitive structures. In this review, the properties, structures, and recent developments regarding the use of the most commonly used nanomaterials in electrochemical sensors designed for pesticide analysis are examined in detail.</div><div>Furthermore, the requirements, structural features, and future development of electrochemical sensors that can be prepared cost-effectively and rapidly for sensitive and selective analysis of fruit pesticide residues have been examined and explained in detail. For this reason, the studies in the literature on pesticide analysis in fruits in the last 5 years are summarized, and some studies are explained in detail. Finally, this study will shed light on the studies on fruit pesticide analysis.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"5 ","pages":"Article 100056"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of experiments-assisted UPLC method for quantification of nitrosamine impurities in glimepiride and lobeglitazone sulfate: A green chemistry approach","authors":"KiranKumar Chagarlamudi ,&nbsp;Venkata Kanaka Srivani Maddala ,&nbsp;Kumaraswamy Gandla","doi":"10.1016/j.jpbao.2025.100078","DOIUrl":"10.1016/j.jpbao.2025.100078","url":null,"abstract":"<div><div>A robust ultra-performance liquid chromatography (UPLC) method was developed and systematically optimized using a Design of Experiments (DoE) approach for the simultaneous quantification of glimepiride, lobeglitazone sulfate, nitrosamine impurity-3 (IMP-3), and impurity-1 (IMP-1) in the marketed LOBG-G1 formulation. Critical method parameters, including organic phase composition, flow rate, and mobile phase pH, were identified through a comprehensive risk assessment and subsequently optimized using a Box-Behnken design. The final chromatographic conditions—50 % organic phase composition, a flow rate of 0.2 mL/min, and a mobile phase pH of 2.6—ensured efficient separation and quantification of all four analytes. The method was validated in accordance with ICH guidelines, demonstrating excellent linearity (r² &gt; 0.999), high accuracy, and precision, with low relative standard deviation values. Stability studies conducted under different stress conditions revealed significant degradation of all four compounds in acidic, alkaline, and oxidative environments. Degradation products were further characterized using LC-MS/MS analysis, confirming their structural identity. In addition to its analytical performance, the method's environmental sustainability was evaluated using multiple green analytical chemistry assessment tools. The DoE-guided UPLC method offers a highly sensitive, selective, and reproducible analytical platform for the detection of nitrosamine impurities in antidiabetic drugs, providing enhanced method understanding while aligning with sustainability principles.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"5 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional high-performance liquid chromatographic determination of serine, threonine and allothreonine enantiomers in mouse tissues and physiological fluids","authors":"Mai Oyaide ,&nbsp;Takeyuki Akita ,&nbsp;Chiharu Ishii ,&nbsp;Masashi Mita ,&nbsp;Kenji Hamase","doi":"10.1016/j.jpbao.2025.100053","DOIUrl":"10.1016/j.jpbao.2025.100053","url":null,"abstract":"<div><div>The amounts of serine (Ser), threonine (Thr) and allothreonine (aThr) enantiomers in 6 tissues (cerebrum, cerebellum, medulla oblongata, pancreas, liver and kidney) and 2 physiological fluids (plasma and urine) of mice were determined using a highly-selective three-dimensional high-performance liquid chromatographic (3D-HPLC) system. The 3D-HPLC system was composed of reversed-phase (Singularity RP18), anion-exchange (Singularity AX) and chiral separation (Singularity CSP-013S) columns, and the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole were determined. To analyze the mouse tissues and physiological fluids, the separation conditions were re-investigated from those reported in our previous study, and the method was validated using the tissue/physiological fluid samples. For Ser, the presence of the <span>d</span>-form could be determined in all the tissues and physiological fluids (1.77 ± 0.09–266.5 ± 11.3 nmol/g or mL). For <span>d</span>-Thr and <span>d</span>-aThr, the presence in the cerebrum and urine was clearly demonstrated (0.28 ± 0.01–1.44 ± 0.56 for <span>d</span>-Thr and 4.63 ± 1.44–6.44 ± 0.36 nmol/g or mL for <span>d</span>-aThr). Trace levels of <span>d</span>-Thr and <span>d</span>-aThr were detected in the other tissues and plasma. The results indicate that the target 3 hydroxy <span>d</span>-amino acids are widely present in mammalian tissues and physiological fluids, and further investigations focusing on their physiological significance are expected.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"5 ","pages":"Article 100053"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New screening and quantification method by UHPLC-MS/MS of 36 new synthetic cathinones in hair. Application to real cases","authors":"Alfredo Fabrizio Lo Faro ,&nbsp;Anastasio Tini ,&nbsp;Giorgi Kobidze ,&nbsp;Giulio Mannocchi ,&nbsp;Massimo Gottardi ,&nbsp;Francesco Paolo Busardò ,&nbsp;Giuseppe Basile ,&nbsp;Bezhan Chakvetadze","doi":"10.1016/j.jpbao.2024.100040","DOIUrl":"10.1016/j.jpbao.2024.100040","url":null,"abstract":"<div><p>A class of β-keto analogs of phenethylamine known as synthetic cathinones has been identified as the most emergent new class of psychoactive chemicals in the last ten years. Synthetic cathinones (SC) are becoming more varied, which represents a serious danger to social security and public health worldwide. In this work, an analytical technique based on UHPLC-MS/MS was developed for the simultaneously measurement of 36 synthetic cathinones and two metabolites in hair keratin samples. The separation was conducted by Atlantis Premier BEH C18 AX (2.5 μm, 2.1 × 100 mm) column using elution with 5 mM ammonium acetate in water with 0.1 % formic acid (v/v) (mobile phase A) and 2 mM ammonium formate in MeOH/Acetonitrile 50/50, +0.1 % formic acid (v/v) (mobile phase B). The analysis time was 11 min. Mass spectrometer was equipped with electrospray ionization (ESI). Multiple reaction monitoring (MRM) mode was used for the detection and quantification of the studied compounds. The methodology was successfully validated according to the Organization Scientific Area Committee guidelines, with linearity (r² ≥ 0.99) from LOQ to 500 pg/mg concentrations for all the compounds investigated. This method was subsequently applied to nine hair samples positive for ten different synthetic cathinones. The most common SCs identified were 3,4-methylenedioxypyrovalerone (3,4-MDPV), in a concentration range 6.0–1000.0 pg/mg, along with alpha-pyrrolidinopentiophenone (α-PHP (54.0 and 554.0 pg/mg, respectively)), followed by the two positional isomers 3-MMC (556.0 and 5000.0 pg/mg) and 4-MMC (11.5 and 448.0 pg/mg). In conclusion, a validated LC-MS/MS method with high specificity was developed offering an easy and affordable sample preparation, and a run time that makes it suitable for use in a high throughput forensic laboratory for the multi-analyte quantification of 36 novel synthetic cathinones and 2 metabolites in hair.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"4 ","pages":"Article 100040"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949771X24000161/pdfft?md5=958a929ad13f40e45bf901f3a1708ff7&pid=1-s2.0-S2949771X24000161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-assisted synthesis of molecularly imprinted polymers for smartphone-based luminescent hemoglobin one-pot detection","authors":"Khadija Karim ,&nbsp;Aziz Amine ,&nbsp;Fabiana Arduini ,&nbsp;Viviana Scognamiglio","doi":"10.1016/j.jpbao.2024.100033","DOIUrl":"10.1016/j.jpbao.2024.100033","url":null,"abstract":"<div><p>This work introduces a novel strategy to selectively and sensitively determine Hemoglobin (Hb) using a microwave-synthesized molecularly imprinted polymer (MIP) and chemiluminescence (CL) as the detection method. Studies conducted on both MIP and non-imprinted polymer (NIP) evidenced that the MIP material exhibits a high adsorption capacity (48 µg/mg) and an imprinted factor of about 3, in addition to a selective adsorption property towards Hb in the presence of other proteins, whose structures are very similar to that of Hb. The high selectivity and sensitivity of the proposed biosensor were also assured by the CL detection, whose mechanism is based on Hb's ability to enhance luminol oxidation in an alkaline medium in the presence of hydrogen peroxide, emitting a blue light whose intensity is related to the Hb concentration. The emitted light intensities from the CL reaction, directly proportional to Hb concentration, were captured and analyzed using a smartphone and RGB Color application, avoiding the use of sophisticated equipment and facilitating the measurement process. In order to streamline and speed up the detection process, all the experimental steps including Hb adsorption on MIP_Hb, supernatant removal, CL reaction, and smartphone readout occur in a single 8-well strip. Therefore, the proposed approach offers one-pot detection for the sensitive (LOD = 0.03 µg.mL⁻¹, equivalent to 1.5 nmol.L⁻¹) and selective determination of Hb in blood samples, for biomedical or forensic applications, through a rapid, low-cost, simple, and affordable approach.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"4 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949771X24000094/pdfft?md5=47c1d8edb35a873e29005abbd8dae6a6&pid=1-s2.0-S2949771X24000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green analytical chemistry and quality by design: A combined approach towards simultaneous determination of Letrozole with its co-administered Zoledronic Acid for cancer patients","authors":"Nourhan A. Abd El-Fatah ,&nbsp;Heba T. Elbalkiny ,&nbsp;Maha A. Hegazy ,&nbsp;Manal Mohammed Fouad ,&nbsp;Ghada M. El-Sayed","doi":"10.1016/j.jpbao.2024.100036","DOIUrl":"10.1016/j.jpbao.2024.100036","url":null,"abstract":"<div><p>Nowadays, breast cancer is the most affecting and globally diagnosed malignancy among women, yet Letrozole (LTZ) was considered the first-line treatment as hormonal anticancer drug. Unfortunately, LTZ develops osteoporosis as a main side effect which was overcome by using the co-administered; Zoledronic Acid (ZDA). Thus, there was a crucial need for this simultaneous quantification innovation, especially there were no any previously reported methods regarding both drugs together. In this study, an integrated framework was conducted between the experimental <em>analytical quality-by-design</em> (AQbD) approach and green analytical chemistry (GAC), emerging sensitive and robust RP-HPLC method. <em>Box-Behnken Design</em> was the developed model for optimizing an isocratic chromatographic separation on C₁₈ Equisil® ODS (4.6 × 250 mm, 5.0 μm) column at ambient temperature, using the mobile phase of 0.1 % aqueous trifluroacetic acid (pH 2.8): acetonitrile (54.5:45.5, v/v), at 1.0 mL/min flow rate with PDA detection at 254.0 nm and 210.0 nm for LTZ and ZDA, respectively. Model statistical and residual plots analysis was significant and normally distributed. Method was fully validated as per ICH guidelines, where good linearity was 0.20–10.00 µg/mL for both drugs in presence of Tadalafil (TDF) as an internal standard, obtaining adequate correlation coefficients <strong>(r)</strong> values. Calculated LOD results were 0.058 and 0.040 µg/mL while calculated LOQ results were 0.175 and 0.122 µg/mL for LTZ and ZDA, respectively. The proposed method was effectively applied on bulk, pharmaceutical dosage forms, and spiked human plasma. Statistical comparison of the anticipated results with the reported ones was performed. Greenness assessment was evaluated using Green Analytical Procedure Index (GAPI) and Analytical Greenness (AGREE) tools; where superiority results were achieved relative to other reported methods. Finally, an EVG method evaluation tool was assessed, and the attained results were represented through its radar chart.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"4 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949771X24000124/pdfft?md5=56ca2229de65e15d44a24040017a532d&pid=1-s2.0-S2949771X24000124-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability indicating RP-HPLC method development and validation for the determination of pyrimethamine in an oral paediatric suspension","authors":"Sahra Muhammad,&nbsp;Zoé Ribier,&nbsp;Guy Benoit,&nbsp;Joelle Bordenave","doi":"10.1016/j.jpbao.2024.100035","DOIUrl":"10.1016/j.jpbao.2024.100035","url":null,"abstract":"<div><p>The aim of this study is to validate a high-performance liquid chromatography (HPLC) – UV assay method for a stability assay of an oral suspension of PYR developed for newborns. Analyses were performed by HPLC with a Kinetex Coreshell C18 column, thermostated at 40°C, coupled to a diode array (λ=230 nm, 280 nm, scan from 190 to 400 nm) with an acetonitrile/methanol/KH₂PO₄ buffer gradient (pH 3, 10 mM). A forced degradation study was performed to validate the stability-indicating character. PYR was subjected to acidic (pH 2) and basic (pH 12) hydrolysis stress, thermal hydrolysis stress at native pH (60°C and 4°C), H₂O₂ oxidation stress (3 % and 15 %) and photolysis stress (UV and natural light) at day 0, 3 and 16. Linearity, specificity, precision, and accuracy of this assay method have been tested. Forced degradation tests demonstrated the stability indicator character of the method and showed that PYR was very sensitive to oxidation, sensitive to light (UV) and insensitive to hydrolysis (thermal and pH). The method of quantification was linear, specific, precise, and accurate according to ICH recommendations. A stability study on the formulation under development will be carried out with this HPLC – UV method.</p></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"4 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949771X24000112/pdfft?md5=2dd1b3f5a2c40d98abdcf2000d2370e3&pid=1-s2.0-S2949771X24000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polymers for pharmaceutical and biomedical analysis","authors":"Mamajan Ovezova ,&nbsp;Fatma Yılmaz ,&nbsp;Ilgım Göktürk ,&nbsp;Kıvılcım Çaktü Güler ,&nbsp;Adil Denizli","doi":"10.1016/j.jpbao.2024.100038","DOIUrl":"10.1016/j.jpbao.2024.100038","url":null,"abstract":"<div><div>Molecularly imprinted polymers (MIPs) are prepared using a technique that allows the formation of binding sites specific to the chosen target molecule. MIPs are materials that can remain stable against changing physical and chemical environmental conditions, possess void structures specific to the target molecule, are easily synthesized, and have a long shelf life. Although initially used primarily for purification and removal processes, MIPs have recently found applications in the medical field as well. In addition to the aforementioned characteristics, their low cost, ease of use, and high accuracy have made them a preferred choice in industrial sectors. MIPs have been widely applied in disease diagnosis and treatment, drug delivery systems, tissue engineering, and industrial drug analysis. Moreover, by integrating with rapidly evolving sensor technologies in addition to traditional methods, MIPs have shortened analysis times, reduced costs, and increased specificity in all conducted analyses. This compilation aims to explain recent studies and advancements in the pharmaceutical field utilizing MIPs, including immunoassays, bioimaging, drug delivery, tissue engineering, cell recognition, and pharmaceutical analyses. Briefly, the scope of this review is to present important scientific and technological advances in molecularly imprinted polymers used for pharmaceutical and biomedical analyses. Although the vast majority of the review focuses on the years 2020–2024, it also addresses current issues and proposed solutions for the use of highly stable molecularly imprinted polymers in pharmaceutical and biomedical analyses since 2010.</div></div>","PeriodicalId":100822,"journal":{"name":"Journal of Pharmaceutical and Biomedical Analysis Open","volume":"4 ","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949771X24000148/pdfft?md5=26145da75313a743edfc24732d240b8d&pid=1-s2.0-S2949771X24000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}