{"title":"Seasonal changes in the ovariuterus of female Scorpio maurus fuscus (Scorpiones; Scorpionidae), and the effect of higher temperature","authors":"Rivka Elias, M.R Warburg, Mira Rosenberg","doi":"10.1016/S0020-7322(99)00035-5","DOIUrl":"10.1016/S0020-7322(99)00035-5","url":null,"abstract":"<div><p>Changes in the ovariuterus of <em>Scorpio maurus fuscus</em> during the period of one year, and under stressful thermal conditions, are described. The ovariuteri of most females in the population contained 1 generation of large, embryonic diverticulae (Ed) and variable numbers of small, bud-like rudimentary diverticulae (Rd) belonging to 3–5 generations. Most females contained 1 or 2 generations of degenerated (Dd), post-partum diverticulae, but some, probably virgins, lacked them. The latter females were significantly (<em>P</em><0.05) smaller than the others. They also contained a significantly higher (<em>P</em><0.0001) number of Rd than is usually found in the other females. Embryonic diverticulae (Ed) of females with 1 generation of Dd (Dd<sub>1</sub>) were significantly longer (<em>P</em><0.05) than Ed of females with 2 generations of Dd (Dd<sub>1</sub>+Dd<sub>2</sub>). The numbers and dimensions of the various diverticulae changed during the year. Under stressful thermal condition, the total number of Dd increased, and these were considerably longer than those observed under normal ambient conditions.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 4","pages":"Pages 337-348"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00035-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86866221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
João A. da Rosa , José M.S. Barata , Mario Cilense , Francisco M. Belda Neto
{"title":"Head morphology of 1st and 5th instar nymphs of Triatoma circummaculata and Triatoma rubrovaria (Hemiptera, Reduviidae)","authors":"João A. da Rosa , José M.S. Barata , Mario Cilense , Francisco M. Belda Neto","doi":"10.1016/S0020-7322(99)00038-0","DOIUrl":"10.1016/S0020-7322(99)00038-0","url":null,"abstract":"<div><p>Morphological structures of the head of 1st and 5th instar nymphs of <em>Triatoma circummaculata</em> and <em>Triatoma rubrovaria</em> were revealed by analysis using scanning electron microscopy (SEM). Differences between 1st and 5th instar nymphs of these two species were observed in the postocular callosity, the number of ommatidia and tapered hair, the small segment between antennal segments, the rostrum third segment and slit lines. These slit lines were different only in the 5th instar. Similarities observed were the presence of tapered hairs in the joints, and the type of sensilla in the antennal segments. Only the 1st instar shows anteclypeus and gena sensilla. The antennal segments comprise the following types of sensilla: basiconica, bristles type I, bristles type II, bristles type III, campaniformia, coeloconica, chemosensilla, placodea, trichobothria and trichoidea. We describe here for the first time six (3+3) sensilla basiconica on the dorsal portion of the first segment of the rostrum.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 4","pages":"Pages 363-375"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00038-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89908384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abbey C. Brockhouse , Harry T. Horner , Tim F. Booth , Bryony C. Bonning
{"title":"Pericardial cell ultrastructure in the tobacco hornworm","authors":"Abbey C. Brockhouse , Harry T. Horner , Tim F. Booth , Bryony C. Bonning","doi":"10.1016/S0020-7322(99)00029-X","DOIUrl":"10.1016/S0020-7322(99)00029-X","url":null,"abstract":"<div><p>The ultrastructure of the pericardial athrocytes of fifth instar tobacco hornworm <em>Manduca sexta</em> L. (Lepidoptera: Sphingidae) was examined by transmission electron microscopy. These cells are highly specialized for the maintenance of hemolymph homeostasis by synthesis and secretion of some proteins, and uptake and degradation of others. We observed a striking radial zonation with numerous labyrinthine channels extending into the cell providing a large surface area for enhanced efficiency of endocytosis. Granular material was imported into the endosomal compartment by fusion of endocytotic vesicles from the basal region. Anatomical evidence supports the hypothesis that proteins are transported from the endosome to the lysosome, by maturation of the early endosome to form the late endosome/lysosome, as opposed to vesicular transport. The presence of catalase in athrocyte lysosomes is reported for the first time. Endocytosis in the athrocytes of insects is compared with endocytosis in vertebrate systems.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 4","pages":"Pages 261-271"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00029-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90345315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giovanna Del Bene , Vittoria Cavallo , Pietro Lupetti , Romano Dallai
{"title":"Fine structure of the salivary glands of Heliothrips haemorrhoidalis (Bouché) (Thysanoptera: Thripidae)","authors":"Giovanna Del Bene , Vittoria Cavallo , Pietro Lupetti , Romano Dallai","doi":"10.1016/S0020-7322(99)00033-1","DOIUrl":"10.1016/S0020-7322(99)00033-1","url":null,"abstract":"<div><p>Salivary glands of <em>Heliothrips haemorrhoidalis</em> consist of two parts: the tubular and the ovoidal glands. The tubular glands have microvillate cells, which are rich in mitochondria, associated with sinuous plasma membrane and produce a watery secretion. The ovoid glands have cells that are rich in rough endoplasmic reticulum and Golgi complexes and produce viscous secretions of proteinaceous material. It is hypothesized that the viscous secretion contains enzymes that could be activated in appropriate ionic conditions provided by the watery secretion. This mechanism could be realized when the two products are aspirated by the cibarial pump, mixing and flowing into the mouthcone before being injected into the plant cells.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 4","pages":"Pages 301-308"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00033-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87803434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Secretory products of the Malpighian tubules of Cicadellidae (Hemiptera, Membracoidea): an ultrastructural study","authors":"R.A Rakitov","doi":"10.1016/S0020-7322(99)00023-9","DOIUrl":"10.1016/S0020-7322(99)00023-9","url":null,"abstract":"<div><p>The middle segment of the Malpighian tubules (MT), specialized for secretion of proteins and lipids, was investigated by transmission electron microscopy in nymphs and adults of nine cicadellid species (Hemiptera, Cicadellidae). In addition, the external cuticle coated with secretory products of the MT was studied by scanning electron microscopy. The ultimate secretory product found in adult cicadellids, both in the MT and on the cuticle, were intricately structured particles known as brochosomes (BS). These showed significant differences in size, shape and internal structure among species studied. Common features of all BS were a bounding outer membrane and regular cell-like depressions on the surface. Nascent BS develop in numerous Golgi regions as secretory vesicles individually enclosed in larger vacuoles. Definitive structure of BS is acquired during the peculiar post-Golgian maturation. Two basic patterns of the secretion in the MT during ontogenesis were recognized. In <em>Cicadella viridis</em> (L.), <em>Bathysmatophorus reuteri</em> J. Shlb.<em>, Graphocraerus ventralis</em> (Fall.) and <em>Doratura impudica</em> Horv. BS are produced and applied onto the cuticle in both adults and nymphs. In contrast, in young nymphs of <em>Vilbasteana oculata</em> (Ldb.), <em>Populicerus nitidissimus</em> (H.-S.), <em>Oncopsis flavicollis</em> (L.) and <em>Ulopa reticulata</em> (F.) the MT are involved in production of various secretions other than BS. The secretory cells in these species undergo a change switching to BS production in the last nymphal instar, so that the BS are applied onto the cuticle for the first time only in freshly molted adults. The secretory patterns differ among subfamilies. The observed diversity of products suggests that the protective water- or feces-repellent function proposed for BS cannot completely explain the adaptive significance of the secretory specialization of the MT in cicadellids.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 179-193"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00023-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88365720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wolfgang Blenau , Manfred Schmidt , Daniel Faensen , Friedrich-Wilhelm Schürmann
{"title":"Neurons with dopamine-like immunoreactivity target mushroom body Kenyon cell somata in the brain of some hymenopteran insects","authors":"Wolfgang Blenau , Manfred Schmidt , Daniel Faensen , Friedrich-Wilhelm Schürmann","doi":"10.1016/S0020-7322(99)00025-2","DOIUrl":"10.1016/S0020-7322(99)00025-2","url":null,"abstract":"<div><p>The mushroom bodies of the insect brain are centers for olfactory and multimodal information processing and they are involved in associative olfactory learning. They are comprised of numerous (340,000 in the bee brain), small (3–8 μm soma diameter) local interneurons, the Kenyon cells. In the brain of honeybees (<em>Apis mellifera</em>) of all castes (worker bees, drones and queens), wasps (<em>Vespula germanica</em>) and hornets (<em>Vespa crabro</em>) immunostaining revealed fibers with dopamine-like immunoreactivity projecting from the pedunculus and the lip neuropil of the mushroom bodies into the Kenyon cell perikaryal layer. These fibers terminate with numerous varicosities, mainly around the border between medial and lateral Kenyon cell soma groups. Visualization of immunostained terminals in the transmission electron microscope showed that they directly contact the somata of the Kenyon cells and contain presynaptic elements. The somata of the Kenyon cells are clearly non-immunoreactive. Synaptic contacts at the somata are unusual for the central nervous systems of insects and other arthropods. This finding suggests that the somata of the Kenyon cells of Hymenoptera may serve an integrative role, and not merely a supportive function.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 203-210"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00025-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73149604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structure and function of seminal vesicles of Orthoptera Tettigonioidea","authors":"Renata Viscuso, Lucia Narcisi, Lorenzo Sottile","doi":"10.1016/S0020-7322(99)00022-7","DOIUrl":"10.1016/S0020-7322(99)00022-7","url":null,"abstract":"<div><p><span><span>The epithelium of seminal vesicles of seven species of Orthoptera </span>Tettigonioidea was examined. Results revealed an epithelium that is always monolayered and devoid of cuticular intima, thus reflecting its mesodermic origin. In some species epithelial secretory activity is intense and seems to be effected via both </span>apocrine and merocrine mechanisms. A peculiar characteristic observed in most of the species examined consists in the presence of spermiophagic activity, never previously recorded in insect seminal vesicles which have usually been attributed the function of synthesizing the substances that maintain sperm viable during their storage therein. The probable role of this spermiophagic activity in relation to the reproductive biology of Orthoptera Tettigonioidea is discussed.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 169-178"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00022-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123134363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ontogenesis of hemocytes in the stick insect Bacillus rossius (Rossi) (Phasmatodea, Bacillidae) studied with an anti-hemocyte monoclonal antibody","authors":"S Meloni, M Mazzini, G Scapigliati","doi":"10.1016/S0020-7322(99)00020-3","DOIUrl":"10.1016/S0020-7322(99)00020-3","url":null,"abstract":"<div><p>The monoclonal antibody BrH1, specific for hemocytes of the stick insect <em>Bacillus rossius</em>, was employed to study the appearance of hemocytes during embryogenesis. Laid eggs were collected for eight weeks, and cryosections were probed with the antibody. First positive cells were detected at the fifth week, and increased in number onwards. No peculiar differences were observed in the overall morphology between embryonic and non-embryonic hemocytes.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 247-252"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00020-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81146060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differentiation of primordial germ cells during embryogenesis of Allacma fusca (L.) (Collembola: Symphypleona)","authors":"Jerzy Klag, Piotr Świa̧tek","doi":"10.1016/S0020-7322(99)00021-5","DOIUrl":"10.1016/S0020-7322(99)00021-5","url":null,"abstract":"<div><p>The youngest primordial germ cells (PGCs) of <em>Allacma fusca</em> (L.) (Collembola: Sminthuridae) can be identified in embryos at the blastoderm stage as scattered in the yolk mass. They are arranged in pairs connected via intercellular bridges and dispersed among the yolk granules over a relatively small area but they never form multicellular clusters. With progressing development, the mesoderm of the germ band differentiates, the PGCs migrate to the abdominal part of the germ band and enter among mesoderm cells making two clusters of cells in the left and right parts of the abdomen. The mesoderm cells neighbouring the PGC cluster differentiate into a one-layered gonad envelope and produce a thin basal lamina separating the gonad from the rest of the mesoderm. The PGCs are still connected in pairs. At the end of the embryonic development, the gonads have regular spherical shapes and are enclosed within the envelope built up by a layer of flat somatic cells. Now, the PGCs do not occur only in pairs, but chains of cells connected with a sequence of intercellular bridges can also be seen.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 161-168"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00021-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75225770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tatiana G.T. Matos, Lilian G. Giugliano, Bergmann M. Ribeiro, Sônia N. Báo
{"title":"Structural and ultrastructural studies of Anticarsia gemmatalis midgut cells infected with the baculovirus A. gemmatalis nucleopolyhedrovirus","authors":"Tatiana G.T. Matos, Lilian G. Giugliano, Bergmann M. Ribeiro, Sônia N. Báo","doi":"10.1016/S0020-7322(99)00024-0","DOIUrl":"10.1016/S0020-7322(99)00024-0","url":null,"abstract":"<div><p><em>Anticarsia gemmatalis</em> is a lepidopteran insect susceptible to <em>A. gemmatalis</em> nucleopolyhedrovirus (AgNPV), which is being used in a large scale, in Brazil, as a biological control agent against this serious soybean pest. Baculovirus usually infects its insect host through the midgut epithelium. In the midgut, it replicates in the nuclei of epithelial cells, producing progeny virus and establishing systemic infection. The AgNPV infection of <em>A. gemmatalis</em> midgut was studied using light and electron microscopy. It was observed that AgNPV enters the midgut mainly through columnar cells. Although the virus was not found in the nuclei of columnar cells until late on infection, it is believed that these cells are the primary sites of infection and replication. This fact can be explained by the continuous regeneration of the midgut epithelium. Besides, the infection may be occurring in isolated cells, making it more difficult to be visualized by electron microscopy. At 48 h post infection, hemocytes and tracheoblasts are infected and polyhedra are formed later in these cells, which are the secondary sites of infection.</p></div>","PeriodicalId":100701,"journal":{"name":"International Journal of Insect Morphology and Embryology","volume":"28 3","pages":"Pages 195-201"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0020-7322(99)00024-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81584489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}